IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Apoptosis signal-regulating kinase 1 controls the proapoptotic function of death-associated protein (Daxx) in the cytoplasm. Apoptosis signal-regulating kinase 1 controls the proapoptotic function of death-associated protein (Daxx) in the cytoplasm. J. Biol. Chem. (0021-9258) 2001 Ko YG., Kang YS., Park H., Seol W., Kim J., Kim T., Park HS., Choi EJ., Kim S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer The source of protein Fas taken from PMID 9990010. The source of protein Daxx taken from PMID 9743501. The source of protein ASK1 taken from PMID 10411906. sungkim@snu.ac.kr 22-Jan-2008: Contacted by JYOTI. davet human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy immunostaining immunostaining Ko YG., Kang YS., Park H., Seol W., Kim J., Kim T., Park HS., Choi EJ., Kim S. Apoptosis - Interactions involving proteins with a function related to apoptosis The source of protein Fas taken from PMID 9990010. The source of protein Daxx taken from PMID 9743501. The source of protein ASK1 taken from PMID 10411906. sungkim@snu.ac.kr Accepted 2007-Not a month!-5 by DAVET. J. Biol. Chem. (0021-9258) 2001 davet Apoptosis signal-regulating kinase 1 controls the proapoptotic function of death-associated protein (Daxx) in the cytoplasm. Apoptosis signal-regulating kinase 1 controls the proapoptotic function of death-associated protein (Daxx) in the cytoplasm. J. Biol. Chem. (0021-9258) 2001 Ko YG., Kang YS., Park H., Seol W., Kim J., Kim T., Park HS., Choi EJ., Kim S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer The source of protein Fas taken from PMID 9990010. The source of protein Daxx taken from PMID 9743501. The source of protein ASK1 taken from PMID 10411906. sungkim@snu.ac.kr 22-Jan-2008: Contacted by JYOTI. davet human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Ko YG., Kang YS., Park H., Seol W., Kim J., Kim T., Park HS., Choi EJ., Kim S. Apoptosis - Interactions involving proteins with a function related to apoptosis The source of protein Fas taken from PMID 9990010. The source of protein Daxx taken from PMID 9743501. The source of protein ASK1 taken from PMID 10411906. sungkim@snu.ac.kr Accepted 2007-Not a month!-5 by DAVET. 22-Jan-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2001 davet Apoptosis signal-regulating kinase 1 controls the proapoptotic function of death-associated protein (Daxx) in the cytoplasm. Apoptosis signal-regulating kinase 1 controls the proapoptotic function of death-associated protein (Daxx) in the cytoplasm. J. Biol. Chem. (0021-9258) 2001 Ko YG., Kang YS., Park H., Seol W., Kim J., Kim T., Park HS., Choi EJ., Kim S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer The source of protein Fas taken from PMID 9990010. The source of protein Daxx taken from PMID 9743501. The source of protein ASK1 taken from PMID 10411906. sungkim@snu.ac.kr 22-Jan-2008: Contacted by JYOTI. davet human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ko YG., Kang YS., Park H., Seol W., Kim J., Kim T., Park HS., Choi EJ., Kim S. Apoptosis - Interactions involving proteins with a function related to apoptosis The source of protein Fas taken from PMID 9990010. The source of protein Daxx taken from PMID 9743501. The source of protein ASK1 taken from PMID 10411906. sungkim@snu.ac.kr Accepted 2007-Not a month!-5 by DAVET. 22-Jan-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2001 davet Apoptosis signal-regulating kinase 1 controls the proapoptotic function of death-associated protein (Daxx) in the cytoplasm. Apoptosis signal-regulating kinase 1 controls the proapoptotic function of death-associated protein (Daxx) in the cytoplasm. J. Biol. Chem. (0021-9258) 2001 Ko YG., Kang YS., Park H., Seol W., Kim J., Kim T., Park HS., Choi EJ., Kim S. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer The source of protein Fas taken from PMID 9990010. The source of protein Daxx taken from PMID 9743501. The source of protein ASK1 taken from PMID 10411906. sungkim@snu.ac.kr 22-Jan-2008: Contacted by JYOTI. davet human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ko YG., Kang YS., Park H., Seol W., Kim J., Kim T., Park HS., Choi EJ., Kim S. Apoptosis - Interactions involving proteins with a function related to apoptosis The source of protein Fas taken from PMID 9990010. The source of protein Daxx taken from PMID 9743501. The source of protein ASK1 taken from PMID 10411906. sungkim@snu.ac.kr Accepted 2007-Not a month!-5 by DAVET. 22-Jan-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2001 davet m3k5_human Mitogen-activated protein kinase kinase kinase 5 MAPK/ERK kinase kinase 5 Apoptosis signal-regulating kinase 1 MAP3K5 ASK1 MAPKKK5 MEKK5 protein protein human Homo sapiens Human MSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKQT DIP protein Q99683 original sequence version: 83 DIP protein Q99683 original sequence version: 151 DIP protein Q99683 original sequence version: 113 265BDC65968AF985 daxx_human Death domain-associated protein 6 DAXX BING2 DAP6 Daxx Fas death domain-associated protein ETS1-associated protein 1 protein protein human Homo sapiens Human MATANSIIVLDDDDEDEAAAQPGPSHPLPNAASPGAEAPSSSEPHGARGSSSSGGKKCYKLENEKLFEEFLELCKMQTADHPEVVPFLYNRQQRAHSLFLASAEFCNILSRVLSRARSRPAKLYVYINELCTVLKAHSAKKKLNLAPAATTSNEPSGNNPPTHLSLDPTNAENTASQSPRTRGSRRQIQRLEQLLALYVAEIRRLQEKELDLSELDDPDSAYLQEARLKRKLIRLFGRLCELKDCSSLTGRVIEQRIPYRGTRYPEVNRRIERLINKPGPDTFPDYGDVLRAVEKAAARHSLGLPRQQLQLMAQDAFRDVGIRLQERRHLDLIYNFGCHLTDDYRPGVDPALSDPVLARRLRENRSLAMSRLDEVISKYAMLQDKSEEGERKKRRARLQGTSSHSADTPEASLDSGEGPSGMASQGCPSASRAETDDEDDEESDEEEEEEEEEEEEEATDSEEEEDLEQMQEGQEDDEEEDEEEEAAAGKDGDKSPMSSLQISNEKNLEPGKQISRSSGEQQNKGRIVSPSLLSEEPLAPSSIDAESNGEQPEELTLEEESPVSQLFELEIEALPLDTPSSVETDISSSRKQSEEPFTTVLENGAGMVSSTSFNGGVSPHNWGDSGPPCKKSRKEKKQTGSGPLGNSYVERQRSVHEKNGKKICTLPSPPSPLASLAPVADSSTRVDSPSHGLVTSSLCIPSPARLSQTPHSQPPRPGTCKTSVATQCDPEEIIVLSDSD 1B309ADDAA878040 tnr6_human Tumor necrosis factor receptor superfamily member 6 FASLG receptor Apoptosis-mediating surface antigen FAS Apo-1 antigen FAS APT1 TNFRSF6 FAS1 protein protein human Homo sapiens Human MLGIWTLLPLVLTSVARLSSKSVNAQVTDINSKGLELRKTVTTVETQNLEGLHHDGQFCHKPCPPGERKARDCTVNGDEPDCVPCQEGKEYTDKAHFSSKCRRCRLCDEGHGLEVEINCTRTQNTKCRCKPNFFCNSTVCEHCDPCTKCEHGIIKECTLTSNTKCKEEGSRSNLGWLCLLLLPIPLIVWVKRKEVQKTCRKHRKENQGSHESPTLNPETVAINLSDVDLSKYITTIAGVMTLSQVKGFVRKNGVNEAKIDEIKNDNVQDTAEQKVQLLRNWHQLHGKKEAYDTLIKDLKKANLCTLAEKIQTIILKDITSDSENSNFRNEIQSLV 0139942535111410 daxx-map3k5-1 1 5 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 1 A Q9UER7; Q99683 transfection daxx-map3k5-2 2 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait physical association physical association 1 B ASK1 was associated with Daxx. map3k5-daxx 3 5 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey physical association physical association 1 B ASK1 was associated with Daxx. fas-daxx-5 4 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 3 Daxx was recruited to Fas in the cells co-expressing both of Daxx and ASK1 whereas it was not in the cells expressing either one of the two proteins, suggesting that the molecular interaction between Daxx and Fas requires ASK1. ASK1 - Daxx was recruited to Fas in cells co-expressing both Daxx and ASK1, whereas it was not in cells only expressing Daxx. Q9UER7 transfection fas-map3k5 2 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait physical association physical association 3 more ASK1 was recruited to Fas after Fas ligation in the cells with Daxx and ASK1 than in the cells with ASK1 alone, implying that Daxx is a mediator for helping the recruitment of ASK1 to Fas after Fas ligation. Daxx - More ASK1 was recruited to Fas in cells transfected with Daxx and ASK1, than in the cells with ASK1 alone. Q99683 transfection These results indicate that Daxx is a mediator for helping the recruitment of ASK1 to Fas.