IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Intracellular association of a mutant insulin-like growth factor receptor with endogenous receptors. Intracellular association of a mutant insulin-like growth factor receptor with endogenous receptors. Clin. Cancer Res. (1078-0432) 2001 Reiss K., Tu X., Romano G., Peruzzi F., Wang JY., Baserga R. Cancer - Interactions investigated in the context of cancer The ranges for the IGF-IR proreceptor and, the alpha and beta subunits were found in the UniProtKB entry P08069. The IGF-IR construct is of human origin as stated in reference 30 (PMID 11320406). r_baserga@lac.jci.tju.edu 13-Nov-2007: Contacted by JYOTI. mouse-p6 Mus musculus P6 cells p6 Mouse P6 cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Reiss K., Tu X., Romano G., Peruzzi F., Wang JY., Baserga R. Cancer - Interactions investigated in the context of cancer The ranges for the IGF-IR proreceptor and, the alpha and beta subunits were found in the UniProtKB entry P08069. The IGF-IR construct is of human origin as stated in reference 30 (PMID 11320406). r_baserga@lac.jci.tju.edu Accepted 2007-NOV-12 by JYOTI. Clin. Cancer Res. (1078-0432) 2001 Intracellular association of a mutant insulin-like growth factor receptor with endogenous receptors. Intracellular association of a mutant insulin-like growth factor receptor with endogenous receptors. Clin. Cancer Res. (1078-0432) 2001 Reiss K., Tu X., Romano G., Peruzzi F., Wang JY., Baserga R. Cancer - Interactions investigated in the context of cancer The ranges for the IGF-IR proreceptor and, the alpha and beta subunits were found in the UniProtKB entry P08069. The IGF-IR construct is of human origin as stated in reference 30 (PMID 11320406). r_baserga@lac.jci.tju.edu 13-Nov-2007: Contacted by JYOTI. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Reiss K., Tu X., Romano G., Peruzzi F., Wang JY., Baserga R. Cancer - Interactions investigated in the context of cancer The ranges for the IGF-IR proreceptor and, the alpha and beta subunits were found in the UniProtKB entry P08069. The IGF-IR construct is of human origin as stated in reference 30 (PMID 11320406). r_baserga@lac.jci.tju.edu Accepted 2007-NOV-12 by JYOTI. 13-Nov-2007: Contacted by JYOTI. Clin. Cancer Res. (1078-0432) 2001 igf1r_human Insulin-like growth factor 1 receptor Insulin-like growth factor I receptor IGF1R protein protein human Homo sapiens Human MKSGSGGGSPTSLWGLLFLSAALSLWPTSGEICGPGIDIRNDYQQLKRLENCTVIEGYLHILLISKAEDYRSYRFPKLTVITEYLLLFRVAGLESLGDLFPNLTVIRGWKLFYNYALVIFEMTNLKDIGLYNLRNITRGAIRIEKNADLCYLSTVDWSLILDAVSNNYIVGNKPPKECGDLCPGTMEEKPMCEKTTINNEYNYRCWTTNRCQKMCPSTCGKRACTENNECCHPECLGSCSAPDNDTACVACRHYYYAGVCVPACPPNTYRFEGWRCVDRDFCANILSAESSDSEGFVIHDGECMQECPSGFIRNGSQSMYCIPCEGPCPKVCEEEKKTKTIDSVTSAQMLQGCTIFKGNLLINIRRGNNIASELENFMGLIEVVTGYVKIRHSHALVSLSFLKNLRLILGEEQLEGNYSFYVLDNQNLQQLWDWDHRNLTIKAGKMYFAFNPKLCVSEIYRMEEVTGTKGRQSKGDINTRNNGERASCESDVLHFTSTTTSKNRIIITWHRYRPPDYRDLISFTVYYKEAPFKNVTEYDGQDACGSNSWNMVDVDLPPNKDVEPGILLHGLKPWTQYAVYVKAVTLTMVENDHIRGAKSEILYIRTNASVPSIPLDVLSASNSSSQLIVKWNPPSLPNGNLSYYIVRWQRQPQDGYLYRHNYCSKDKIPIRKYADGTIDIEEVTENPKTEVCGGEKGPCCACPKTEAEKQAEKEEAEYRKVFENFLHNSIFVPRPERKRRDVMQVANTTMSSRSRNTTAADTYNITDPEELETEYPFFESRVDNKERTVISNLRPFTLYRIDIHSCNHEAEKLGCSASNFVFARTMPAEGADDIPGPVTWEPRPENSIFLKWPEPENPNGLILMYEIKYGSQVEDQRECVSRQEYRKYGGAKLNRLNPGNYTARIQATSLSGNGSWTDPVFFYVQAKTGYENFIHLIIALPVAVLLIVGGLVIMLYVFHRKRNNSRLGNGVLYASVNPEYFSAADVYVPDEWEVAREKITMSRELGQGSFGMVYEGVAKGVVKDEPETRVAIKTVNEAASMRERIEFLNEASVMKEFNCHHVVRLLGVVSQGQPTLVIMELMTRGDLKSYLRSLRPEMENNPVLAPPSLSKMIQMAGEIADGMAYLNANKFVHRDLAARNCMVAEDFTVKIGDFGMTRDIYETDYYRKGGKGLLPVRWMSPESLKDGVFTTYSDVWSFGVVLWEIATLAEQPYQGLSNEQVLRFVMEGGLLDKPDNCPDMLFELMRMCWQYNPKMRPSFLEIISSIKEEMEPGFREVSFYYSEENKLPEPEELDLEPENMESVPLDPSASSSSLPLPDRHSGHKAENGPGPGVLVLRASFDERQPYAHMNGGRKNERALPLPQSSTC DIP protein P08069 original sequence version: 170 DIP protein P08069 original sequence version: 109 DIP protein P08069 original sequence version: 139 DIP protein P08069 original sequence version: 185 AE8A735F87F745C8 igf1r-igf1r 1 3 unspecified role unspecified role prey prey c-terminal IGF-IRbeta sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey region IGF-IRalpha sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 3 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait region 486/STOP sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association 3 A, B P6 cells constitutively overexpress P08069. P08069 transfection The fraction of IGF-IR that coprecipitates with 486/STOP is very small. P6 cells, which overecpress IGF-IR, have about 5 E5 receptors/cell. Densitometric measurements of blots under different conditions indicate that the fraction of IGF-IR coprecipitating with 486/STOP is less than 5%. igf1r-igf1r-1 2 3 unspecified role unspecified role prey prey c-terminal IGF-IRbeta sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain mouse-p6 Mus musculus P6 cells p6 Mouse P6 cells 3 unspecified role unspecified role bait bait region 486/STOP sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mouse-r- Mouse R- cells (embryonic fibroblasts lacking type-1 IGF receptor) r- Mouse R- cells (embryonic fibroblasts lacking the type-1 IGF receptor ) Mouse embryonic fibroblasts lacking the type-1 IGF receptor. physical association physical association 8 P6 cells constitutively overexpress P08069. P08069 transfection In these experiments, p6 cells were incubated for 6 h with CM containing 486/STOP as described for Fig. 7. Cell lysates were made and immunoprecipitated with anti-FLAG antibody, and the blots were developed with an antibody to the b subunit of the IGF-IR. Lanes 2 and 3 show that the b subunit of the IGF-IR coprecipitates with an anti-FLAG antibody, and the amount precipitated is proportional to the amount of 486/STOP in the CM