IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Evi-1 transforming and repressor activities are mediated by CtBP co-repressor proteins. Evi-1 transforming and repressor activities are mediated by CtBP co-repressor proteins. J. Biol. Chem. (0021-9258) 2001 imex curation Palmer S., Brouillet JP., Kilbey A., Fulton R., Walker M., Crossley M., Bartholomew C. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer c.bartholomew@gcal.ac.uk 07-Sept-2007: Contacted by JYOTI. orchard Only protein-protein interactions human-bosc_23 Homo sapiens embryonic kidney cell line BOSC-23 bosc_23 Human embryonic kidney cell line BOSC-23 The BOSC-23 cell line are a derivative of the 293T cell line. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Palmer S., Brouillet JP., Kilbey A., Fulton R., Walker M., Crossley M., Bartholomew C. Apoptosis - Interactions involving proteins with a function related to apoptosis c.bartholomew@gcal.ac.uk Accepted 2007-SEP-30 by ORCHARD. 07-Sept-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2001 Only protein-protein interactions imex curation Evi-1 transforming and repressor activities are mediated by CtBP co-repressor proteins. Evi-1 transforming and repressor activities are mediated by CtBP co-repressor proteins. J. Biol. Chem. (0021-9258) 2001 imex curation Palmer S., Brouillet JP., Kilbey A., Fulton R., Walker M., Crossley M., Bartholomew C. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer c.bartholomew@gcal.ac.uk 07-Sept-2007: Contacted by JYOTI. orchard Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Palmer S., Brouillet JP., Kilbey A., Fulton R., Walker M., Crossley M., Bartholomew C. Apoptosis - Interactions involving proteins with a function related to apoptosis c.bartholomew@gcal.ac.uk Accepted 2007-SEP-31 by ORCHARD. 07-Sept-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2001 orchard Only protein-protein interactions imex curation ctbp2_mouse C-terminal-binding protein 2 Ctbp2 protein protein mouse Mus musculus Mouse MALVDKHKVKRQRLDRICEGIRPQIMNGPLHPRPLVALLDGRDCTVEMPILKDLATVAFCDAQSTQEIHEKVLNEAVGAMMYHTITLTREDLEKFKALRVIVRIGSGYDNVDIKAAGELGIAVCNIPSAAVEETADSTVCHILNLYRRNTWLYQALREGTRVQSVEQIREVASGAARIRGETLGLIGFGRTGQAVAVRAKAFGFSVIFYDPYLQDGIERSLGVQRVYTLQDLLYQSDCVSLHCNLNEHNHHLINDFTIKQMRQGAFLVNAARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFAQGPLKDAPNLICTPHTAWYSEQASLEMREAAATEIRRAITGRIPESLRNCVNKEFFVTSAPWSVIDQQAIHPELNGATYRYPPGIVGVAPGGLPPAMEGIIPGGIPVTHNLPTVAHPSQAPSPNQPTKHGDNREHPNEQ 5A81B9D75A9E493B mecom_human Histone-lysine N-methyltransferase MECOM MECOM EVI1 Ecotropic virus integration site 1 protein homolog MDS1 Myelodysplasia syndrome 1 protein Myelodysplasia syndrome-associated protein 1 MDS1 and EVI1 complex locus protein PRDM3 protein protein human Homo sapiens Human MRSKGRARKLATNNECVYGNYPEIPLEEMPDADGVASTPSLNIQEPCSPATSSEAFTPKEGSPYKAPIYIPDDIPIPAEFELRESNMPGAGLGIWTKRKIEVGEKFGPYVGEQRSNLKDPSYGWEILDEFYNVKFCIDASQPDVGSWLKYIRFAGCYDQHNLVACQINDQIFYRVVADIAPGEELLLFMKSEDYPHETMAPDIHEERQYRCEDCDQLFESKAELADHQKFPCSTPHSAFSMVEEDFQQKLESENDLQEIHTIQECKECDQVFPDLQSLEKHMLSHTEEREYKCDQCPKAFNWKSNLIRHQMSHDSGKHYECENCAKVFTDPSNLQRHIRSQHVGARAHACPECGKTFATSSGLKQHKHIHSSVKPFICEVCHKSYTQFSNLCRHKRMHADCRTQIKCKDCGQMFSTTSSLNKHRRFCEGKNHFAAGGFFGQGISLPGTPAMDKTSMVNMSHANPGLADYFGANRHPAGLTFPTAPGFSFSFPGLFPSGLYHRPPLIPASSPVKGLSSTEQTNKSQSPLMTHPQILPATQDILKALSKHPSVGDNKPVELQPERSSEERPFEKISDQSESSDLDDVSTPSGSDLETTSGSDLESDIESDKEKFKENGKMFKDKVSPLQNLASINNKKEYSNHSIFSPSLEEQTAVSGAVNDSIKAIASIAEKYFGSTGLVGLQDKKVGALPYPSMFPLPFFPAFSQSMYPFPDRDLRSLPLKMEPQSPGEVKKLQKGSSESPFDLTTKRKDEKPLTPVPSKPPVTPATSQDQPLDLSMGSRSRASGTKLTEPRKNHVFGGKKGSNVESRPASDGSLQHARPTPFFMDPIYRVEKRKLTDPLEALKEKYLRPSPGFLFHPQMSAIENMAEKLESFSALKPEASELLQSVPSMFNFRAPPNALPENLLRKGKERYTCRYCGKIFPRSANLTRHLRTHTGEQPYRCKYCDRSFSISSNLQRHVRNIHNKEKPFKCHLCDRCFGQQTNLDRHLKKHENGNMSGTATSSPHSELESTGAILDDKEDAYFTEIRNFIGNSNHGSQSPRNVEERMNGSHFKDEKALVTSQNSDLLDDEEVEDEVLLDEEDEDNDITGKTGKEPVTSNLHEGNPEDDYEETSALEMSCKTSPVRYKEEEYKSGLSALDHIRHFTDSLKMRKMEDNQYSEAELSSFSTSHVPEELKQPLHRKSKSQAYAMMLSLSDKESLHSTSHSSSNVWHSMARAAAESSAIQSISHV DIP protein Q03112 original sequence version: 129 49E9B7CBCD422042 evi1-ctbp2 2 3 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait region Rp required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region site a binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region site b binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association 1 A, B, C, 4 A, B pKGIRp produces a GAL4 DNA-binding domain (DBD)Rp protein which interacts with mCtBP2 in yeast AH109 cells Deletion of site a of Evi-1 (aa 553-559) impairs this interaction but deletion of site b of Evi-1 (aa 584-590) abolishes this interaction. ctbp2-evi1 1 3 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 2 B Western blot analysis of anti-FLAG M2-immunoprecipitated cell extracts, with anti-myc 9E10, detects the Evi1myc fusion protein only in cell extracts containing both Evi1myc and FLAGmCtBP2 epitope-tagged proteins (Fig. 2B), demonstrating the two proteins can interact in mammalian cells. P56546; Q03112 transfection