IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Maximal aryl hydrocarbon receptor activity depends on an interaction with the retinoblastoma protein. Maximal aryl hydrocarbon receptor activity depends on an interaction with the retinoblastoma protein. Mol. Pharmacol. (0026-895X) 2001 imex curation Elferink CJ., Ge NL., Levine A. Cancer - Interactions investigated in the context of cancer cornelis_elferink@wayne.edu 27-NOV-2006: Contacted by JYOTI. Returned mail. Only protein-protein interactions imex curation yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast two hybrid array two hybrid array predetermined predetermined participant predetermined Elferink CJ., Ge NL., Levine A. Cancer - Interactions investigated in the context of cancer cornelis_elferink@wayne.edu Accepted 2006-NOV-16 by JYOTI. 27-NOV-2006: Contacted by JYOTI. Returned mail. Mol. Pharmacol. (0026-895X) 2001 IMEx Maximal aryl hydrocarbon receptor activity depends on an interaction with the retinoblastoma protein. Maximal aryl hydrocarbon receptor activity depends on an interaction with the retinoblastoma protein. Mol. Pharmacol. (0026-895X) 2001 imex curation Elferink CJ., Ge NL., Levine A. Cancer - Interactions investigated in the context of cancer cornelis_elferink@wayne.edu 27-NOV-2006: Contacted by JYOTI. Returned mail. Only protein-protein interactions imex curation rat-bp8 Rattus norvegicus AhR defective hepatoma L5 cells bp8 rat hepatoma AhR defective L5 cells These cells are aryl hydrocarbon receptor (AhR) defective rat hepatoma L5 cells. These cells are rat hepatoma L5 cells that are defective in the Aryl hydrocarbon receptor (AhR). anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Elferink CJ., Ge NL., Levine A. Cancer - Interactions investigated in the context of cancer cornelis_elferink@wayne.edu Accepted 2006-NOV-16 by JYOTI. 27-NOV-2006: Contacted by JYOTI. Returned mail. Mol. Pharmacol. (0026-895X) 2001 IMEx rb_human Retinoblastoma-associated protein pRb pp110 p105-Rb RB1 p110-RB1 protein protein human Homo sapiens Human MPPKTPRKTAATAAAAAAEPPAPPPPPPPEEDPEQDSGPEDLPLVRLEFEETEEPDFTALCQKLKIPDHVRERAWLTWEKVSSVDGVLGGYIQKKKELWGICIFIAAVDLDEMSFTFTELQKNIEISVHKFFNLLKEIDTSTKVDNAMSRLLKKYDVLFALFSKLERTCELIYLTQPSSSISTEINSALVLKVSWITFLLAKGEVLQMEDDLVISFQLMLCVLDYFIKLSPPMLLKEPYKTAVIPINGSPRTPRRGQNRSARIAKQLENDTRIIEVLCKEHECNIDEVKNVYFKNFIPFMNSLGLVTSNGLPEVENLSKRYEEIYLKNKDLDARLFLDHDKTLQTDSIDSFETQRTPRKSNLDEEVNVIPPHTPVRTVMNTIQQLMMILNSASDQPSENLISYFNNCTVNPKESILKRVKDIGYIFKEKFAKAVGQGCVEIGSQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSTSQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKAEGNLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDREGPTDHLESACPLNLPLQNNHTAADMYLSPVRSPKKKGSTTRVNSTANAETQATSAFQTQKPLKSTSLSLFYKKVYRLAYLRLNTLCERLLSEHPELEHIIWTLFQHTLQNEYELMRDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAVQETFKRVLIKEEEYDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFPSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGSNPPKPLKKLRFDIEGSDEADGSKHLPGESKFQQKLAEMTSTRTRMQKQKMNDSMDTSNKEEK DIP protein P06400 original sequence version: 102 DIP protein P06400 original sequence version: 195 DIP protein P06400 original sequence version: 204 DIP protein P06400 original sequence version: 126 DIP protein P06400 original sequence version: 151 DIP protein P06400 original sequence version: 153 DIP protein P06400 original sequence version: 181 DIP protein P06400 original sequence version: 184 DIP protein P06400 original sequence version: 213 DIP protein P06400 original sequence version: 208 DIP protein P06400 original sequence version: 174 DIP protein P06400 original sequence version: 212 DIP protein P06400 original sequence version: 179 DIP protein P06400 original sequence version: 146 C8E746111E19CC32 ahr_rat Aryl hydrocarbon receptor Ahr protein protein rat Rattus norvegicus (Rat) Rat MSSGANITYASRKRRKPVQKTVKPVPAEGIKSNPSKRHRDRLNTELDRLASLLPFPQDVINKLDKLSVLRLSVSYLRAKSFFDVALKSTPADRSRGQDQCRAQVRDWQDLQEGEFLLQALNGFVLVVTADALVFYASSTIQDYLGFQQSDVIHQSVYELIHTEDRAEFQRQLHWALNPSQCTDSAQGVDETHGLPQPAVYYTPDQLPPENTAFMERCFRCRLRCLLDNSSGFLAMNFQGRLKYLHGQNKKGKDGALLPPQLALFAIATPLQPPSILEIRTKNFIFRTKHKLDFTPIGCDAKGQLILGYTEVELCNKGSGYQFIHAADMLHCAESHIRMIKTGESGMTVFRLLAKHSRWRWVQSNARLIYRNGRPDYIIATQRPLTDEEGREHLQKRSMTLPFMFATGEAVLYEISSPFSPIMDPLPIRTKSNTSRKDWAPQSTPSKDSFHPNSLMSALIQQDESIYLCPPSSPAPLDSHFLMDSMSECGSWQGSFAVASNEALLKHEEIRHTQDVNLTLSGGPSELFPDNKNNDLYSIMRNLGIDFEDIRSMQNEEFFRTDSSGEVDFKDIDITDEILTYVQDSLNNSTLLNSACQQQPVSQHLSCMLQERLQLEQQQQLQQQHPTQTLEPQRQLCQVEVPQHELGQKTKHMQVNGMFASWNPAPPVSFSCPQQERKHYSLFSGLQGTAQEFPYKSEVDSMPYTQNFAPCNQSLLPEHSKGTQLDFPGRDFERSLHPNASNLEDFVSCLQVPENQRHGINSQSAMVSPQAYYAGAMSMYQCQAGPQHTPVDQMQYSPEIPGSQAFLSKFQSPSILNEAYSADLSSIGHLQTAAHLPRLAEAQPLPDITPSGFL 4EED0EE795FF1327 arnt_rat Aryl hydrocarbon receptor nuclear translocator Dioxin receptor, nuclear translocator Hypoxia-inducible factor 1-beta Arnt protein protein rat Rattus norvegicus (Rat) Rat MAATTANPEMTSDVPSLGPTIASGNPGPGIQGGGAVVQRAIKRRSGLDFDDEGEVNSKFLRCDDEQMCNDKERFARSDDEQSSADKERLARENHSEIERRRRNKMTAYITELSDMVPTCSALARKPDKLTILRMAVSHMKSLRGTGNTSTDGSYKPSFLTDQELKHLILEAADGFLFIVSCETGRVVYVSDSVTPVLNQPQSEWFGSTLYDQVHPDDVDKLREQLSTSENALTGRILDLKTGTVKKEGQQSSMRMCMGSRRSFICRMRCGTSSVDPVSMNRLSFLRNRCRNGLGSVKEGEPHFVVVHCTGYIKAWPPAGVSLPDDDPEAGQGSKFCLVAIGRLQVTSSPNCTDMSNICQPTEFISRHNIEGIFTFVDHRCVATVGYQPQELLGKNIVEFCHPEDQQLLRDSFQQVVKLKGQVLSVMFRFRAKNREWLWMRTSSFTFQNPYSDEMSIFICTNTNVKNSSQEPRPTLSNTIQRSQLGPTTNLSLEMGTGVLASRQQQQQQQQQQQQQQQQTELDMVPGRDGLASYSHSQVSVQPVATAGSEHSKPLEKSEGLFVQDRDPRFSEIYPNISADQSKGLSSSTVPATQQLFSQGSSFPPNPRPAENFRNSGLTPPVTIVQPSSSAGQILAQISRHSNLTQGSAPTWTSSTRPGFSAQLPTQATAKTRSSQFGVNNFQTSSSFSAMSLPGAPTASPSTAAYPTLPNRGSNFPPETGQTTGQFQTRTAEGVGVWPQWQGQQPHHRSSSNEQHVQPTSAQPSSQPEVFQEMLSMLGDQSNTYNNEEFPDLTMFPPFSE CD71FCDF2C23BF34 ahr_human Aryl hydrocarbon receptor AHR BHLHE76 Class E basic helix-loop-helix protein 76 protein protein human Homo sapiens Human MNSSSANITYASRKRRKPVQKTVKPIPAEGIKSNPSKRHRDRLNTELDRLASLLPFPQDVINKLDKLSVLRLSVSYLRAKSFFDVALKSSPTERNGGQDNCRAANFREGLNLQEGEFLLQALNGFVLVVTTDALVFYASSTIQDYLGFQQSDVIHQSVYELIHTEDRAEFQRQLHWALNPSQCTESGQGIEEATGLPQTVVCYNPDQIPPENSPLMERCFICRLRCLLDNSSGFLAMNFQGKLKYLHGQKKKGKDGSILPPQLALFAIATPLQPPSILEIRTKNFIFRTKHKLDFTPIGCDAKGRIVLGYTEAELCTRGSGYQFIHAADMLYCAESHIRMIKTGESGMIVFRLLTKNNRWTWVQSNARLLYKNGRPDYIIVTQRPLTDEEGTEHLRKRNTKLPFMFTTGEAVLYEATNPFPAIMDPLPLRTKNGTSGKDSATTSTLSKDSLNPSSLLAAMMQQDESIYLYPASSTSSTAPFENNFFNESMNECRNWQDNTAPMGNDTILKHEQIDQPQDVNSFAGGHPGLFQDSKNSDLYSIMKNLGIDFEDIRHMQNEKFFRNDFSGEVDFRDIDLTDEILTYVQDSLSKSPFIPSDYQQQQSLALNSSCMVQEHLHLEQQQQHHQKQVVVEPQQQLCQKMKHMQVNGMFENWNSNQFVPFNCPQQDPQQYNVFTDLHGISQEFPYKSEMDSMPYTQNFISCNQPVLPQHSKCTELDYPMGSFEPSPYPTTSSLEDFVTCLQLPENQKHGLNPQSAIITPQTCYAGAVSMYQCQPEPQHTHVGQMQYNPVLPGQQAFLNKFQNGVLNETYPAELNNINNTQTTTHLQPLHHPSEARPFPDLTSSGFL DIP protein P35869 original sequence version: 109 1BFE022871B7B028 rb_rat Retinoblastoma-associated protein pRb pp105 Rb1 Rb-1 protein protein rat Rattus norvegicus (Rat) Rat MPPKAPRRTAAAEPPPPPPPPPEDDPAQDSDPEELPLIRLEFEKIEEPEFIALCQKLKVPDHVRERAWLTWEKVSSVDGILEGYIQKKKELWGICIFIAAVDLDEMPFTFTELQKSIETSVYKFFDLLKEIDTSTKVDNAVSRLLKKYNVLCALYSKLERTCGLIYLTQPSSGLSTEINSMLVLKVSWITFLLAKGEVVQMEDDLVISFQLMLCVLDYFIKLSPPALLREPYKTAATPINGSPRTPRRGQNRSARIAKQLESDTRTIEVLCKEHECNVDEVKNVYFKNFIPFISSLGIVSSNGLPELESLSKRYEEVYLKSKDLDARLFLDHDKTLQTDTIDSFETERTPRKSNPDEEANMVTPHTPVRTVMNTIQQLMVILNSASDQPSENLISYFSNCTVNPKENILKRVKDVGHIFKEKFASAVGQGCIDIGAQRYKLGVRLYYRVMESMLKSEEERLSIQNFSKLLNDNIFHMSLLACALEVVMATYSRSMLQNLDSGTDLSFPWILNVLNLKAFDFYKVIESFIKVEANLTREMIKHLERCEHRIMESLAWLSDSPLFDLIKQSKDGEGPDHLESACSLSLPLQSNHTAADMYLSPIRSPKKRTSTTRVNSAANTETQAASAFHTQKPLKSTSLSLFYKKVYRLAYLRLNTLCARLLSDHPELEHIIWTLFQHTLENEYELMKDRHLDQIMMCSMYGICKVKNIDLKFKIIVTAYKDLPHAAQETFKRVLIREEEFDSIIVFYNSVFMQRLKTNILQYASTRPPTLSPIPHIPRSPYKFSSSPLRIPGGNIYISPLKSPYKISEGLPTPTKMTPRSRILVSIGESFGTSEKFQKINQMVCNSDRVLKRSAEGGNPPKPLKKLRFDIEGSDEADGSKHLPAESKFQQKLAEMTSTRTRMQKQKLNDSMEISNKEEK 3BA735EA59927301 ahr-rb1-2 1 3 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 12 10 physical association physical association 1 Growth of cells 666 Elferink et al. containing the C-terminal truncated AhR constructs required cotransformation with pB42ADpRb These results indicate that the bHLH domain of AhR is not required for interaction with pRb. ahr-rb1-1 1 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P35869:p.Leu331Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L A Wed May 02 19:02:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu331ala' Tue Jun 30 17:35:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu331Ala' P35869:p.Cys333Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain C A Tue Jun 30 18:27:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys333Ala' P35869:p.Glu335Ala mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain E A Wed May 02 14:46:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu335ala' Tue Jun 30 19:16:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu335Ala' 3 unspecified role unspecified role prey prey c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 15 20 physical association physical association 2 coexpression of pRb fails to support cell growth of cells containing the mutated AhR constructs implying that the AhR-pRb interaction requires each of the conserved amino acids within the LXCXE motif. These mutations in pRb also stopped growth when the AhR117-672 construct was used. These results indicate the pRb interaction with AhR requires the mutated residues which are conserved residues in the LXCXE motif (a consensus pRb binding sequence). ahr-arnt-7 2 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait P41738:p.Glu333Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A Wed May 02 15:44:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu333ala' Tue Jun 30 19:13:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu333Ala' association association We show here that an anti-AhR antibody can coprecipitate pRb and the Arnt protein in a TCDD-dependent manner from the virally infected BP8 cells (compare lanes 3 and 4). In contrast, although the mutant AhR in BP8-E333A cells binds the Arnt protein, it failed to associate with pRb. 7 P41738 endogenous The interaction is dependent upon the presence of TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin)(CHEBI:28119). ahr-arnt-6 2 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait P41738:p.Glu333Ala mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A Wed May 02 19:15:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu333ala' Tue Jun 30 19:52:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu333Ala' physical association physical association We show here that an anti-AhR antibody can coprecipitate pRb and the Arnt protein in a TCDD-dependent manner from the virally infected BP8 cells (compare lanes 3 and 4). In contrast, although the mutant AhR in BP8-E333A cells binds the Arnt protein, it failed to associate with pRb. 7 P41738 endogenous The interaction is dependent upon the presence of TCDD (2,3,7,8-tetrachlorodibenzo-p-dioxin)(CHEBI:28119).