IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. J. Biol. Chem. (0021-9258) 2000 imex curation Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology Cancer - Interactions investigated in the context of cancer A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. pklinken@cyllene.uwa.edu.au 07-NOV-2006: Contacted by JYOTI. Only protein-protein interactions mouse-me17 Murine erythroid cell line me17 Murine erythroid cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. 2A pklinken@cyllene.uwa.edu.au Accepted 2006-MAY-10 by JYOTI. 07-NOV-2006: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. J. Biol. Chem. (0021-9258) 2000 imex curation Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology Cancer - Interactions investigated in the context of cancer A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. pklinken@cyllene.uwa.edu.au 07-NOV-2006: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid full dna sequence full identification by DNA sequencing full dna sequence Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. pklinken@cyllene.uwa.edu.au Accepted 2006-MAY-10 by JYOTI. 07-NOV-2006: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. J. Biol. Chem. (0021-9258) 2000 imex curation Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology Cancer - Interactions investigated in the context of cancer A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. pklinken@cyllene.uwa.edu.au 07-NOV-2006: Contacted by JYOTI. Only protein-protein interactions mouse-c11 p53-/- mouse fetal liver cells c11 p53-/- mouse fetal liver cells Cells were p53 negative and were transformed with a myc-raf virus. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. 2A pklinken@cyllene.uwa.edu.au Accepted 2006-MAY-10 by JYOTI. 07-NOV-2006: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. J. Biol. Chem. (0021-9258) 2000 imex curation Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology Cancer - Interactions investigated in the context of cancer A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. pklinken@cyllene.uwa.edu.au 07-NOV-2006: Contacted by JYOTI. Only protein-protein interactions mouse-erythroleukemia Mus musculus me17-1 Murine erythroid cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. 2A pklinken@cyllene.uwa.edu.au Accepted 2006-MAY-10 by JYOTI. 07-NOV-2006: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. J. Biol. Chem. (0021-9258) 2000 imex curation Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology Cancer - Interactions investigated in the context of cancer A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. pklinken@cyllene.uwa.edu.au 07-NOV-2006: Contacted by JYOTI. Only protein-protein interactions mouse-j2e Mouse fetal liver cells j2e Murine fetal liver cell line In vitro infection of murine fetal liver cells with a retrovirus containing v-raf and v-myc oncogenes has produced continuous lines of immature erythroid cells that are leukemogenic. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. 2 pklinken@cyllene.uwa.edu.au Accepted 2006-MAY-10 by JYOTI. 07-NOV-2006: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. J. Biol. Chem. (0021-9258) 2000 imex curation Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology Cancer - Interactions investigated in the context of cancer A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. pklinken@cyllene.uwa.edu.au 07-NOV-2006: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. 1D,E pklinken@cyllene.uwa.edu.au Accepted 2006-MAY-10 by JYOTI. 07-NOV-2006: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. J. Biol. Chem. (0021-9258) 2000 imex curation Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology Cancer - Interactions investigated in the context of cancer A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. pklinken@cyllene.uwa.edu.au 07-NOV-2006: Contacted by JYOTI. Only protein-protein interactions mouse-j2e Mouse fetal liver cells j2e Murine fetal liver cell line In vitro infection of murine fetal liver cells with a retrovirus containing v-raf and v-myc oncogenes has produced continuous lines of immature erythroid cells that are leukemogenic. imaging technique imaging technique microscopy immunostaining immunostaining Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. 2D pklinken@cyllene.uwa.edu.au Accepted 2006-MAY-10 by JYOTI. 07-NOV-2006: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. J. Biol. Chem. (0021-9258) 2000 imex curation Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology Cancer - Interactions investigated in the context of cancer A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. pklinken@cyllene.uwa.edu.au 07-NOV-2006: Contacted by JYOTI. Only protein-protein interactions mouse-c24 p53-/- mouse fetal liver cells c24 p53-/- mouse fetal liver cells Cells were p53 negative and were transformed with a myc-raf virus. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. 2A pklinken@cyllene.uwa.edu.au Accepted 2006-MAY-10 by JYOTI. 07-NOV-2006: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. HS1 interacts with Lyn and is critical for erythropoietin-induced differentiation of erythroid cells. J. Biol. Chem. (0021-9258) 2000 imex curation Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology Cancer - Interactions investigated in the context of cancer A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. pklinken@cyllene.uwa.edu.au 07-NOV-2006: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Ingley E., Sarna MK., Beaumont JG., Tilbrook PA., Tsai S., Takemoto Y., Williams JH., Klinken SP. BioCreative - Critical Assessment of Information Extraction systems in Biology A cDNA library from lymphohemopoietic progenitor cell line EML C.1 was used. 1B,C pklinken@cyllene.uwa.edu.au Accepted 2006-MAY-10 by JYOTI. 07-NOV-2006: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2000 Only protein-protein interactions imex curation lck_mouse Proto-oncogene tyrosine-protein kinase LCK Lck Lsk-t Lymphocyte cell-specific protein-tyrosine kinase p56-LCK Leukocyte C-terminal Src kinase protein protein mouse Mus musculus Mouse MGCVCSSNPEDDWMENIDVCENCHYPIVPLDSKISLPIRNGSEVRDPLVTYEGSLPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHDLVRHYTNASDGLCTKLSRPCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHPRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLNVNKLLDMAAQIAEGMAFIEEQNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYHLMMLCWKERPEDRPTFDYLRSVLDDFFTATEGQYQPQP 3513102F49A7FD0B hcls1_mouse Hematopoietic lineage cell-specific protein Hcls1 Hs1 Hematopoietic cell-specific LYN substrate 1 LckBP1 protein protein mouse Mus musculus Mouse MWKSVVGHDVSVSVETQGDDWDTDPDFVNDISEKEQRWGAKTIEGSGRTEHINIHQLRNKVSEEHDILKKKELESGPKASHGYGGRFGVERDRMDKSAVGHEYVADVEKHSSQTDAARGFGGKYGVERDRADKSAVGFDYKGEVEKHASQKDYSHGFGGRYGVEKDKRDKAALGYDYKGETEKHESQRDYAKGFGGQYGIQKDRVDKSAVGFNEMEAPTTAYKKTTPIEAASSGARGLKAKFESLAEEKRKREEEEKAQQMARQQQERKAVVKMSREVQQPSMPVEEPAAPAQLPKKISSEVWPPAESHLPPESQPVRSRREYPVPSLPTRQSPLQNHLEDNEEPPALPPRTPEGLQVVEEPVYEAAPELEPEPEPDYEPEPETEPDYEDVGELDRQDEDAEGDYEDVLEPEDTPSLSYQAGPSAGAGGAGISAIALYDYQGEGSDELSFDPDDIITDIEMVDEGWWRGQCRGHFGLFPANYVKLL 0EE14FEFA0A31412 lyn_mouse Tyrosine-protein kinase Lyn Lyn V-yes-1 Yamaguchi sarcoma viral related oncogene homolog p53Lyn p56Lyn protein protein mouse Mus musculus Mouse MGCIKSKRKDNLNDDEVDSKTQPVRNTDRTIYVRDPTSNKQQRPVPEFHLLPGQRFQTKDPEEQGDIVVALYPYDGIHPDDLSFKKGEKMKVLEEHGEWWKAKSLSSKREGFIPSNYVAKVNTLETEEWFFKDITRKDAERQLLAPGNSAGAFLIRESETLKGSFSLSVRDYDPMHGDVIKHYKIRSLDNGGYYISPRITFPCISDMIKHYQKQSDGLCRRLEKACISPKPQKPWDKDAWEIPRESIKLVKKLGAGQFGEVWMGYYNNSTKVAVKTLKPGTMSVQAFLEEANLMKTLQHDKLVRLYAVVTKEEPIYIITEFMAKGSLLDFLKSDEGGKVLLPKLIDFSAQIAEGMAYIERKNYIHRDLRAANVLVSESLMCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGCFTIKSDVWSFGILLYEIVTYGKIPYPGRTNADVMSALSQGYRMPRMENCPDELYDIMKMCWKEKAEERPTFDYLQSVLDDFYTATEGQYQQQP DIP protein P25911 original sequence version: 163 DIP protein P25911 original sequence version: 118 FDA1D21CC90C50EC fyn_mouse Tyrosine-protein kinase Fyn p59-Fyn Fyn Proto-oncogene c-Fyn protein protein mouse Mus musculus Mouse MGCVQCKDKEAAKLTEERDGSLNQSSGYRYGTDPTPQHYPSFGVTSIPNYNNFHAAGGQGLTVFGGVNSSSHTGTLRTRGGTGVTLFVALYDYEARTEDDLSFHKGEKFQILNSSEGDWWEARSLTTGETGYIPSNYVAPVDSIQAEEWYFGKLGRKDAERQLLSFGNPRGTFLIRESETTKGAYSLSIRDWDDMKGDHVKHYKIRKLDNGGYYITTRAQFETLQQLVQHYSERAAGLCCRLVVPCHKGMPRLTDLSVKTKDVWEIPRESLQLIKRLGNGQFGEVWMGTWNGNTKVAIKTLKPGTMSPESFLEEAQIMKKLKHDKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKDGEGRALKLPNLVDMAAQVAAGMAYIERMNYIHRDLRSANILVGNGLICKIADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELVTKGRVPYPGMNNREVLEQVERGYRMPCPQDCPISLHELMIHCWKKDPEERPTFEYLQGFLEDYFTATEPQYQPGENL 99558702596DAEE0 lyn-hs1-2 2 11 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role bait bait physical association physical association hs1-lyn-2 9 12 unspecified role unspecified role prey prey ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction lyn-hs1-7 6 12 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association lyn-hs1-11 5 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association hs1-lyn-3 5 12 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait physical association physical association Maximal coimmunoprecipitation of Lyn occurred between 15 and 30 minutes of erythropoietin stimulation. Erythropoietin. hs1-lck-1 5 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association hs1-fyn-1 5 13 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association lyn-hs1-12 3 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association lyn-hs1-13 8 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association lyn-hs1-14 4 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association lyn-hs1-15 1 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association hs1-lyn-4 7 12 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization