MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Activation of tyrosine kinase c-Abl contributes to α-synuclein-induced neurodegeneration. Activation of tyrosine kinase c-Abl contributes to α-synuclein-induced neurodegeneration. The Journal of clinical investigation 2016 imex curation Brahmachari S., Ge P., Lee SH., Kim D., Karuppagounder SS., Kumar M., Mao X., Shin JH., Lee Y., Pletnikova O., Troncoso JC., Dawson VL., Dawson TM., Ko HS. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Brain Disease - Neurological Diseases & Disorders From PMID: 16899465 c-ABL-KA and c-ABL-KD are inferred to be of human origin. recombinant c-ABL is inferred to be be human according to datasheet. hko3@jhmi.edu Only protein-protein interactions imex curation mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Brahmachari S., Ge P., Lee SH., Kim D., Karuppagounder SS., Kumar M., Mao X., Shin JH., Lee Y., Pletnikova O., Troncoso JC., Dawson VL., Dawson TM., Ko HS. Parkinsons - Interactions investigated in the context of Parkinsons disease From PMID: 16899465 c-ABL-KA and c-ABL-KD are inferred to be of human origin. recombinant c-ABL is inferred to be be human according to datasheet. hko3@jhmi.edu Accepted 2021-OCT-18 AT 11:27 BST AT 11:27 BST by MGT The Journal of clinical investigation 2016 Only protein-protein interactions imex curation imex curation Activation of tyrosine kinase c-Abl contributes to α-synuclein-induced neurodegeneration. Activation of tyrosine kinase c-Abl contributes to α-synuclein-induced neurodegeneration. The Journal of clinical investigation 2016 imex curation Brahmachari S., Ge P., Lee SH., Kim D., Karuppagounder SS., Kumar M., Mao X., Shin JH., Lee Y., Pletnikova O., Troncoso JC., Dawson VL., Dawson TM., Ko HS. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Brain Disease - Neurological Diseases & Disorders From PMID: 16899465 c-ABL-KA and c-ABL-KD are inferred to be of human origin. recombinant c-ABL is inferred to be be human according to datasheet. hko3@jhmi.edu Only protein-protein interactions imex curation mouse-brainstem mouse-brainstem brainstem brainstem confocal microscopy confocal microscopy immunohistochemistry immunohistochemistry Brahmachari S., Ge P., Lee SH., Kim D., Karuppagounder SS., Kumar M., Mao X., Shin JH., Lee Y., Pletnikova O., Troncoso JC., Dawson VL., Dawson TM., Ko HS. Parkinsons - Interactions investigated in the context of Parkinsons disease From PMID: 16899465 c-ABL-KA and c-ABL-KD are inferred to be of human origin. recombinant c-ABL is inferred to be be human according to datasheet. hko3@jhmi.edu Accepted 2021-OCT-18 AT 11:27 BST AT 11:27 BST by MGT The Journal of clinical investigation 2016 Only protein-protein interactions imex curation imex curation Activation of tyrosine kinase c-Abl contributes to α-synuclein-induced neurodegeneration. Activation of tyrosine kinase c-Abl contributes to α-synuclein-induced neurodegeneration. The Journal of clinical investigation 2016 imex curation Brahmachari S., Ge P., Lee SH., Kim D., Karuppagounder SS., Kumar M., Mao X., Shin JH., Lee Y., Pletnikova O., Troncoso JC., Dawson VL., Dawson TM., Ko HS. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Brain Disease - Neurological Diseases & Disorders From PMID: 16899465 c-ABL-KA and c-ABL-KD are inferred to be of human origin. recombinant c-ABL is inferred to be be human according to datasheet. hko3@jhmi.edu Only protein-protein interactions imex curation mouse-brainstem mouse-brainstem brainstem brainstem anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Brahmachari S., Ge P., Lee SH., Kim D., Karuppagounder SS., Kumar M., Mao X., Shin JH., Lee Y., Pletnikova O., Troncoso JC., Dawson VL., Dawson TM., Ko HS. Parkinsons - Interactions investigated in the context of Parkinsons disease From PMID: 16899465 c-ABL-KA and c-ABL-KD are inferred to be of human origin. recombinant c-ABL is inferred to be be human according to datasheet. hko3@jhmi.edu Accepted 2021-OCT-18 AT 11:27 BST AT 11:27 BST by MGT The Journal of clinical investigation 2016 Only protein-protein interactions imex curation imex curation Activation of tyrosine kinase c-Abl contributes to α-synuclein-induced neurodegeneration. Activation of tyrosine kinase c-Abl contributes to α-synuclein-induced neurodegeneration. The Journal of clinical investigation 2016 imex curation Brahmachari S., Ge P., Lee SH., Kim D., Karuppagounder SS., Kumar M., Mao X., Shin JH., Lee Y., Pletnikova O., Troncoso JC., Dawson VL., Dawson TM., Ko HS. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Brain Disease - Neurological Diseases & Disorders From PMID: 16899465 c-ABL-KA and c-ABL-KD are inferred to be of human origin. recombinant c-ABL is inferred to be be human according to datasheet. hko3@jhmi.edu Only protein-protein interactions imex curation human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Brahmachari S., Ge P., Lee SH., Kim D., Karuppagounder SS., Kumar M., Mao X., Shin JH., Lee Y., Pletnikova O., Troncoso JC., Dawson VL., Dawson TM., Ko HS. Parkinsons - Interactions investigated in the context of Parkinsons disease From PMID: 16899465 c-ABL-KA and c-ABL-KD are inferred to be of human origin. recombinant c-ABL is inferred to be be human according to datasheet. hko3@jhmi.edu Accepted 2021-OCT-18 AT 11:27 BST AT 11:27 BST by MGT The Journal of clinical investigation 2016 Only protein-protein interactions imex curation imex curation Activation of tyrosine kinase c-Abl contributes to α-synuclein-induced neurodegeneration. Activation of tyrosine kinase c-Abl contributes to α-synuclein-induced neurodegeneration. The Journal of clinical investigation 2016 imex curation Brahmachari S., Ge P., Lee SH., Kim D., Karuppagounder SS., Kumar M., Mao X., Shin JH., Lee Y., Pletnikova O., Troncoso JC., Dawson VL., Dawson TM., Ko HS. Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Brain Disease - Neurological Diseases & Disorders From PMID: 16899465 c-ABL-KA and c-ABL-KD are inferred to be of human origin. recombinant c-ABL is inferred to be be human according to datasheet. hko3@jhmi.edu Only protein-protein interactions imex curation in vitro In vitro in gel kinase assay in-gel kinase assay in gel kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Brahmachari S., Ge P., Lee SH., Kim D., Karuppagounder SS., Kumar M., Mao X., Shin JH., Lee Y., Pletnikova O., Troncoso JC., Dawson VL., Dawson TM., Ko HS. Parkinsons - Interactions investigated in the context of Parkinsons disease From PMID: 16899465 c-ABL-KA and c-ABL-KD are inferred to be of human origin. recombinant c-ABL is inferred to be be human according to datasheet. hko3@jhmi.edu Accepted 2021-OCT-18 AT 11:27 BST AT 11:27 BST by MGT The Journal of clinical investigation 2016 Only protein-protein interactions imex curation imex curation syua_mouse Alpha-synuclein Snca Syn Non-A beta component of AD amyloid Non-A4 component of amyloid precursor protein protein mouse Mus musculus Mouse MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVTTVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQMGKGEEGYPQEGILEDMPVDPGSEAYEMPSEEGYQDYEPEA 1FFD19D7E15E636C syua_human Alpha-synuclein Non-A beta component of AD amyloid Non-A4 component of amyloid precursor SNCA NACP PARK1 protein protein human Homo sapiens Human MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA DIP protein P37840 original sequence version: 124 DIP protein P37840 original sequence version: 175 DIP protein P37840 original sequence version: 178 6BB2F12128931663 abl1_mouse Tyrosine-protein kinase ABL1 Abl1 Abl Abelson murine leukemia viral oncogene homolog 1 Proto-oncogene c-Abl p150 Abelson tyrosine-protein kinase 1 protein protein mouse Mus musculus Mouse MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTIYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVSAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKRGTRGGAGSMLQAPELPTKTRTCRRAAEQKDAPDTPELLHTKGLGESDALDSEPAVSPLLPRKERGPPDGSLNEDERLLPRDRKTNLFSALIKKKKKMAPTPPKRSSSFREMDGQPDRRGASEDDSRELCNGPPALTSDAAEPTKSPKASNGAGVPNGAFREPGNSGFRSPHMWKKSSTLTGSRLAAAEEESGMSSSKRFLRSCSASCMPHGARDTEWRSVTLPRDLPSAGKQFDSSTFGGHKSEKPALPRKRTSESRSEQVAKSTAMPPPRLVKKNEEAAEEGFKDTESSPGSSPPSLTPKLLRRQVTASPSSGLSHKEEATKGSASGMGTPATAEPAPPSNKVGLSKASSEEMRVRRHKHSSESPGRDKGRLAKLKPAPPPPPACTGKAGKPAQSPSQEAGEAGGPTKTKCTSLAMDAVNTDPTKAGPPGEGLRKPVPPSVPKPQSTAKPPGTPTSPVSTPSTAPAPSPLAGDQQPSSAAFIPLISTRVSLRKTRQPPERIASGTITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLESNLRELQICPATASSGPAATQDFSKLLSSVKEISDIVRR BD48ADE8557AE95C abl1_human Tyrosine-protein kinase ABL1 Abelson tyrosine-protein kinase 1 ABL1 ABL JTK7 Abelson murine leukemia viral oncogene homolog 1 Proto-oncogene c-Abl p150 protein protein human Homo sapiens Human MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTSRRAAEHRDTTDVPEMPHSKGQGESDPLDHEPAVSPLLPRKERGPPEGGLNEDERLLPKDKKTNLFSALIKKKKKTAPTPPKRSSSFREMDGQPERRGAGEEEGRDISNGALAFTPLDTADPAKSPKPSNGAGVPNGALRESGGSGFRSPHLWKKSSTLTSSRLATGEEEGGGSSSKRFLRSCSASCVPHGAKDTEWRSVTLPRDLQSTGRQFDSSTFGGHKSEKPALPRKRAGENRSDQVTRGTVTPPPRLVKKNEEAADEVFKDIMESSPGSSPPNLTPKPLRRQVTVAPASGLPHKEEAGKGSALGTPAAAEPVTPTSKAGSGAPGGTSKGPAEESRVRRHKHSSESPGRDKGKLSRLKPAPPPPPAASAGKAGGKPSQSPSQEAAGEAVLGAKTKATSLVDAVNSDAAKPSQPGEGLKKPVLPATPKPQSAKPSGTPISPAPVPSTLPSASSALAGDQPSSTAFIPLISTRVSLRKTRQPPERIASGAITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLENNLRELQICPATAGSGPAATQDFSKLLSSVKEISDIVQR DIP protein P00519 original sequence version: 114 DIP protein P00519 original sequence version: 137 DIP protein P00519 original sequence version: 161 DIP protein P00519 original sequence version: 220 DIP protein P00519 original sequence version: 219 85FE6C1C0E483EA2 syn-abl-1 4 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P00519:p.Lys291Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K R 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed Myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 6A snca-abl1-2 1 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed P00519:p.Lys291Arg mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K R 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P37840:p.Ala53Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A T physical association physical association Fig. 6C snca-abl1-3 5 9 enzyme enzyme neutral component neutral component purified purified 7 enzyme target enzyme target substrate substrate neutral component neutral component purified purified phosphorylated tyrosine optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy autoradiography autoradiography modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain resulting-ptm resulting-ptm resulting ptm resulting-ptm phosphorylation phosphorylation reaction phosphorylation Fig. 6D-6E snca-abl1-4 3 6 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 6B snca-abl1-5 2 7 unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell P37840:p.Ala53Thr mutation mutation certain certain sequence position certain certain certain sequence position certain A T 8 unspecified role unspecified role neutral component neutral component physiological level physiological level endogenous level endogenous endogenous endogenous level fixed cell fixed cell phospho- Tyr optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 1H snca-abl1-6 1 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed P37840:p.Ala53Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A T 8 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association Fig. 6C