IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ IkappaB kinase-alpha is critical for interferon-alpha production induced by Toll-like receptors 7 and 9. IkappaB kinase-alpha is critical for interferon-alpha production induced by Toll-like receptors 7 and 9. Nature (0028-0836) 2006 imex curation Hoshino K., Sugiyama T., Matsumoto M., Tanaka T., Saito M., Hemmi H., Ohara O., Akira S., Kaisho T. BioCreative - Critical Assessment of Information Extraction systems in Biology tkaisho@rcai.riken.jp 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Hoshino K., Sugiyama T., Matsumoto M., Tanaka T., Saito M., Hemmi H., Ohara O., Akira S., Kaisho T. BioCreative - Critical Assessment of Information Extraction systems in Biology tkaisho@rcai.riken.jp Accepted 2006-SEP-13 by JYOTI. Nature (0028-0836) 2006 Only protein-protein interactions imex curation IkappaB kinase-alpha is critical for interferon-alpha production induced by Toll-like receptors 7 and 9. IkappaB kinase-alpha is critical for interferon-alpha production induced by Toll-like receptors 7 and 9. Nature (0028-0836) 2006 imex curation Hoshino K., Sugiyama T., Matsumoto M., Tanaka T., Saito M., Hemmi H., Ohara O., Akira S., Kaisho T. BioCreative - Critical Assessment of Information Extraction systems in Biology tkaisho@rcai.riken.jp 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions mouse-bmdc Mus musculus bone marrow-derived dendritic cells bmdm-1 bone marrow-derived macrophage anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hoshino K., Sugiyama T., Matsumoto M., Tanaka T., Saito M., Hemmi H., Ohara O., Akira S., Kaisho T. BioCreative - Critical Assessment of Information Extraction systems in Biology tkaisho@rcai.riken.jp Accepted 2006-SEP-13 by JYOTI. 24-JAN-2007: Contacted by JYOTI. Nature (0028-0836) 2006 Only protein-protein interactions imex curation ikkb_mouse Inhibitor of nuclear factor kappa-B kinase subunit beta I-kappa-B kinase 2 Nuclear factor NF-kappa-B inhibitor kinase beta Ikbkb Ikkb Serine/threonine protein kinase IKBKB protein protein mouse Mus musculus Mouse MSWSPSLPTQTCGAWEMKERLGTGGFGNVIRWHNQATGEQIAIKQCRQELSPKNRNRWCLEIQIMRRLNHPNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRRYLNQFENCCGLREGAVLTLLSDIASALRYLHENRIIHRDLKPENIVLQQGEKRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHSKVRQKSEVDIVVSEDLNGAVKFSSSLPFPNNLNSVLAERLEKWLQLMLMWHPRQRGTDPQYGPNGCFRALDDILNLKLVHVLNMVTGTVHTYPVTEDESLQSLKTRIQENTGILETDQELLQKAGLVLLPDKPATQCISDSKTNEGLTLDMDLVFLLDNSKINYETQITPRPPPESVSCILQEPKRNLSFFQLRKVWGQVWHSIQTLKEDCNRLQQGQRAAMMSLLRNNSCLSKMKNAMASTAQQLKAKLDFFKTSIQIDLEKYKEQTEFGITSDKLLLAWREMEQAVEQCGRENDVKHLVERMMALQTDIVDLQRSPMGRKQGGTLDDLEEQARELYRKLREKPRDQRTEGDSQEMVRLLLQAIQSFEKKVRVIYTQLSKTVVCKQKALELLPKVEEVVSLMNEDERTVVRLQEKRQKELWNLLKIACSKVRGPVSGSPDSMNVSRLSHPGQLMSQPSSACDSLPESDKKSEELVAEAHALCSRLESALQDTVKEQDRSFTTLDWSWLQMEDEERCSLEQACD DIP protein O88351 original sequence version: 126 DIP protein O88351 original sequence version: 73 DIP protein O88351 original sequence version: 99 DIP protein O88351 original sequence version: 114 DIP protein O88351 original sequence version: 149 FED962F095449C5E ikka_mouse Inhibitor of nuclear factor kappa-B kinase subunit alpha I-kappa-B kinase 1 Conserved helix-loop-helix ubiquitous kinase Nuclear factor NF-kappa-B inhibitor kinase alpha Chuk Ikka protein protein mouse Mus musculus Mouse MERPPGLRPGAGGPWEMRERLGTGGFGNVSLYQHRELDLKIAIKSCRLELSSKNRERWCHEIQIMKKLDHANVVKACDVPEELNFLINDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKTIHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMTGEVRFSSHLPQPNSLCSLIVEPMESWLQLMLNWDPQQRGGPIDLTLKQPRCFALMDHILNLKIVHILNMTSAKIISFLLPCDESLHSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLEFFRKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYSEVGVIGYLEDQIMSLHTEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPPDHLYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGTVTPPVSAWLPPTLADREHPLTCVVTPQDGETLAQMIEENLNCLGHLSTIIREANEDQSSSLMSLDWSWLAE DIP protein Q60680 original sequence version: 82 DIP protein Q60680 original sequence version: 86 DIP protein Q60680 original sequence version: 113 DIP protein Q60680 original sequence version: 126 DIP protein Q60680 original sequence version: 158 3FEF5582AFF92233 irf7_mouse Interferon regulatory factor 7 Irf7 protein protein mouse Mus musculus Mouse MAEVRGVQRVLFGDWLLGEVSSGQYEGLQWLNEARTVFRVPWKHFGRRDLDEEDAQIFKAWAVARGRWPPSGVNLPPPEAEAAERRERRGWKTNFRCALHSTGRFILRQDNSGDPVDPHKVYELSRELGSTVGPATENREEVSLSNALPTQGVSPGSFLARENAGLQTPSPLLSSDAGDLLLQVLQYSHILESESGADPVPPQAPGQEQDRVYEEPYAAWQVEAVPSPRPQQPALTERSLGFLDVTIMYKGRTVLQAVVGHPRCVFLYSPMAPAVRTSEPQPVIFPSPAELPDQKQLHYTETLLQHVSPGLQLELRGPSLWALRMGKCKVYWEVGSPMGTTGPSTPPQLLERNRHTPIFDFSTFFRELEEFRARRRQGSPHYTIYLGFGQDLSAGRPKEKTLILVKLEPWVCKAYLEGVQREGVSSLDSSSLGLCLSSTNSLYEDIEHFLMDLGQWP DIP protein P70434 original sequence version: 118 30B102C668F56142 nemo_mouse NF-kappa-B essential modulator NF-kappa-B essential modifier Inhibitor of nuclear factor kappa-B kinase subunit gamma IkB kinase-associated protein 1 Ikbkg Nemo protein protein mouse Mus musculus Mouse MNKHPWKNQLSEMVQPSGGPAEDQDMLGEESSLGKPAMLHLPSEQGTPETLQRCLEENQELRDAIRQSNQMLRERCEELLHFQVSQREEKEFLMCKFQEARKLVERLSLEKLDLRSQREQALKELEQLKKCQQQMAEDKASVKAQVTSLLGELQESQSRLEAATKDRQALEGRIRAVSEQVRQLESEREVLQQQHSVQVDQLRMQNQSVEAALRMERQAASEEKRKLAQLQAAYHQLFQDYDSHIKSSKGMQLEDLRQQLQQAEEALVAKQELIDKLKEEAEQHKIVMETVPVLKAQADIYKADFQAERHAREKLVEKKEYLQEQLEQLQREFNKLKVGCHESARIEDMRKRHVETPQPPLLPAPAHHSFHLALSNQRRSPPEEPPDFCCPKCQYQAPDMDTLQIHVMECIE DIP protein O88522 original sequence version: 115 DIP protein O88522 original sequence version: 63 DIP protein O88522 original sequence version: 91 DIP protein O88522 original sequence version: 90 DIP protein O88522 original sequence version: 104 DIP protein O88522 original sequence version: 138 66C693C857A2D5E6 chuk-irf7 1 5 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 C In 293T cells, IKK-a co-immunoprecipitated with IRF-7 This association was significantly enhanced by coexpression of MyD88 or TRAF6. chuk-irf7-1 1 4 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 C In 293T cells, IKK-a co-immunoprecipitated with IRF-7 This association was significantly enhanced by coexpression of MyD88 or TRAF6. chuk-irf7-2 2 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association 3 D, E, Supp. Fig. 5 B in Flt3L-induced BMDCs and IRF-7- expressing BMDCs induced with granulocyte�macrophage colonystimulating factor (GM-CSF), IKK-a was detected in IRF-7 immunoprecipitates, but not in control immunoprecipitates (Fig. 3d and Supplementary Fig. 5a). This association was observed before stimulation; after stimulation with TLR7/9 agonists, the amount of co-immunoprecipitated IKK-a increased, and was accompanied by an increase in IRF-7 protein levels This association was observed before stimulation; after stimulation with PolyU or D19, the amount of co-immunoprecipitated IKK-alpha increased, and was accompanied by an increase in IRF-7 protein levels. ikbkb-irf7-3 2 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 3 D, E, Supp. Fig. 5 B IKK-b was also co-immunoprecipitatied in anti-IRF-7 immunoprecipitates, and the co-immunoprecipitated amount increased after stimulation The interaction was stimulated by PolyU or D19. The interaction is very weak without stimulation of cells with PolyU or D19. ikbkg-irf7 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 3 D, E IKK-b was also co-immunoprecipitatied in anti-IRF-7 immunoprecipitates, and the co-immunoprecipitated amount increased after stimulation; in contrast, IKK-g was hardly detected. IKK-gamma did immunoprecipitate with IRF-7 before stimulation to a larger extent than IKK-beta. IKK-gamma also immunoprecipitated after stimulation of the cells with PolyU and D19 but the amount decreased with time.