IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Chromogranin-mediated secretion of mutant superoxide dismutase proteins linked to amyotrophic lateral sclerosis. Chromogranin-mediated secretion of mutant superoxide dismutase proteins linked to amyotrophic lateral sclerosis. Nat. Neurosci. (1097-6256) 2006 imex curation Urushitani M., Sik A., Sakurai T., Nukina N., Takahashi R., Julien JP. BioCreative - Critical Assessment of Information Extraction systems in Biology Synapse - Interactions of proteins with an established role in the presynapse. The gly93ala, ala4val,gly37arg and gly85arg SOD1 mutations have been entered using sequence as stated in UniProt ID= P00441, as gly94ala, ala5val,gly38arg and gly86arg; assuming the N-terminal methionine was not included in the numbering used in this publication. julien@crchul.ulaval.ca 24-JAN-2007: Contacted by JYOTI. Mouse cDNA library from transgenic mouse transformed with mutant SOD (G39A) protein. Only protein-protein interactions mouse-neuro_2_a Mus musculus albino neuroblastoma cell line neuro 2 a (N2a) neuro_2_a Mouse Albino neuroblastoma neuro 2 a cell line fluorescence imaging fluorescence microscopy fluorescence imaging antibody detection identification by antibody antibody detection Urushitani M., Sik A., Sakurai T., Nukina N., Takahashi R., Julien JP. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells from spinal neurons of transgenic (expressing SOD1 gly37arg) or wild type mice were used in this assay. julien@crchul.ulaval.ca Accepted 2006-SEP-13 by JYOTI. 24-JAN-2007: Contacted by JYOTI. Mouse cDNA library from transgenic mouse transformed with mutant SOD (G39A) protein. Nat. Neurosci. (1097-6256) 2006 Only protein-protein interactions imex curation Chromogranin-mediated secretion of mutant superoxide dismutase proteins linked to amyotrophic lateral sclerosis. Chromogranin-mediated secretion of mutant superoxide dismutase proteins linked to amyotrophic lateral sclerosis. Nat. Neurosci. (1097-6256) 2006 imex curation Urushitani M., Sik A., Sakurai T., Nukina N., Takahashi R., Julien JP. BioCreative - Critical Assessment of Information Extraction systems in Biology Synapse - Interactions of proteins with an established role in the presynapse. The gly93ala, ala4val,gly37arg and gly85arg SOD1 mutations have been entered using sequence as stated in UniProt ID= P00441, as gly94ala, ala5val,gly38arg and gly86arg; assuming the N-terminal methionine was not included in the numbering used in this publication. julien@crchul.ulaval.ca 24-JAN-2007: Contacted by JYOTI. Mouse cDNA library from transgenic mouse transformed with mutant SOD (G39A) protein. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Urushitani M., Sik A., Sakurai T., Nukina N., Takahashi R., Julien JP. BioCreative - Critical Assessment of Information Extraction systems in Biology julien@crchul.ulaval.ca Accepted 2006-SEP-13 by JYOTI. Mouse cDNA library from transgenic mouse spinal cord cell, carrying human mutant SOD (G39A) protein. Nat. Neurosci. (1097-6256) 2006 Only protein-protein interactions imex curation Chromogranin-mediated secretion of mutant superoxide dismutase proteins linked to amyotrophic lateral sclerosis. Chromogranin-mediated secretion of mutant superoxide dismutase proteins linked to amyotrophic lateral sclerosis. Nat. Neurosci. (1097-6256) 2006 imex curation Urushitani M., Sik A., Sakurai T., Nukina N., Takahashi R., Julien JP. BioCreative - Critical Assessment of Information Extraction systems in Biology Synapse - Interactions of proteins with an established role in the presynapse. The gly93ala, ala4val,gly37arg and gly85arg SOD1 mutations have been entered using sequence as stated in UniProt ID= P00441, as gly94ala, ala5val,gly38arg and gly86arg; assuming the N-terminal methionine was not included in the numbering used in this publication. julien@crchul.ulaval.ca 24-JAN-2007: Contacted by JYOTI. Mouse cDNA library from transgenic mouse transformed with mutant SOD (G39A) protein. Only protein-protein interactions mouse-neuro_2_a Mus musculus albino neuroblastoma cell line neuro 2 a (N2a) neuro_2_a Mouse Albino neuroblastoma neuro 2 a cell line anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Urushitani M., Sik A., Sakurai T., Nukina N., Takahashi R., Julien JP. BioCreative - Critical Assessment of Information Extraction systems in Biology julien@crchul.ulaval.ca Accepted 2006-SEP-13 by JYOTI. 24-JAN-2007: Contacted by JYOTI. Nat. Neurosci. (1097-6256) 2006 Only protein-protein interactions imex curation Chromogranin-mediated secretion of mutant superoxide dismutase proteins linked to amyotrophic lateral sclerosis. Chromogranin-mediated secretion of mutant superoxide dismutase proteins linked to amyotrophic lateral sclerosis. Nat. Neurosci. (1097-6256) 2006 imex curation Urushitani M., Sik A., Sakurai T., Nukina N., Takahashi R., Julien JP. BioCreative - Critical Assessment of Information Extraction systems in Biology Synapse - Interactions of proteins with an established role in the presynapse. The gly93ala, ala4val,gly37arg and gly85arg SOD1 mutations have been entered using sequence as stated in UniProt ID= P00441, as gly94ala, ala5val,gly38arg and gly86arg; assuming the N-terminal methionine was not included in the numbering used in this publication. julien@crchul.ulaval.ca 24-JAN-2007: Contacted by JYOTI. Mouse cDNA library from transgenic mouse transformed with mutant SOD (G39A) protein. Only protein-protein interactions mouse Mus musculus Mouse electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy antibody detection identification by antibody antibody detection Urushitani M., Sik A., Sakurai T., Nukina N., Takahashi R., Julien JP. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells from spinal neurons of transgenic (expressing SOD1 gly37arg) or wild type mice were used in this assay. julien@crchul.ulaval.ca Accepted 2006-SEP-13 by JYOTI. 24-JAN-2007: Contacted by JYOTI. Mouse cDNA library from transgenic mouse transformed with mutant SOD (G39A) protein. Nat. Neurosci. (1097-6256) 2006 Only protein-protein interactions imex curation Chromogranin-mediated secretion of mutant superoxide dismutase proteins linked to amyotrophic lateral sclerosis. Chromogranin-mediated secretion of mutant superoxide dismutase proteins linked to amyotrophic lateral sclerosis. Nat. Neurosci. (1097-6256) 2006 imex curation Urushitani M., Sik A., Sakurai T., Nukina N., Takahashi R., Julien JP. BioCreative - Critical Assessment of Information Extraction systems in Biology Synapse - Interactions of proteins with an established role in the presynapse. The gly93ala, ala4val,gly37arg and gly85arg SOD1 mutations have been entered using sequence as stated in UniProt ID= P00441, as gly94ala, ala5val,gly38arg and gly86arg; assuming the N-terminal methionine was not included in the numbering used in this publication. julien@crchul.ulaval.ca 24-JAN-2007: Contacted by JYOTI. Mouse cDNA library from transgenic mouse transformed with mutant SOD (G39A) protein. Only protein-protein interactions mouse-neuron Mus musculus neuronal cell neuron Neuron [Neuronal cell; Neural cell fluorescence imaging fluorescence microscopy fluorescence imaging antibody detection identification by antibody antibody detection Urushitani M., Sik A., Sakurai T., Nukina N., Takahashi R., Julien JP. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells from spinal neurons of transgenic (expressing SOD1 gly37arg) or wild type mice were used in this assay. julien@crchul.ulaval.ca Accepted 2006-SEP-13 by JYOTI. 24-JAN-2007: Contacted by JYOTI. Mouse cDNA library from transgenic mouse transformed with mutant SOD (G39A) protein. Nat. Neurosci. (1097-6256) 2006 Only protein-protein interactions imex curation Chromogranin-mediated secretion of mutant superoxide dismutase proteins linked to amyotrophic lateral sclerosis. Chromogranin-mediated secretion of mutant superoxide dismutase proteins linked to amyotrophic lateral sclerosis. Nat. Neurosci. (1097-6256) 2006 imex curation Urushitani M., Sik A., Sakurai T., Nukina N., Takahashi R., Julien JP. BioCreative - Critical Assessment of Information Extraction systems in Biology Synapse - Interactions of proteins with an established role in the presynapse. The gly93ala, ala4val,gly37arg and gly85arg SOD1 mutations have been entered using sequence as stated in UniProt ID= P00441, as gly94ala, ala5val,gly38arg and gly86arg; assuming the N-terminal methionine was not included in the numbering used in this publication. julien@crchul.ulaval.ca 24-JAN-2007: Contacted by JYOTI. Mouse cDNA library from transgenic mouse transformed with mutant SOD (G39A) protein. Only protein-protein interactions mouse Mus musculus Mouse density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Urushitani M., Sik A., Sakurai T., Nukina N., Takahashi R., Julien JP. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells from spinal neurons of transgenic (expressing SOD1 gly37arg) or wild type mice were used in this assay. julien@crchul.ulaval.ca Accepted 2006-SEP-13 by JYOTI. 24-JAN-2007: Contacted by JYOTI. Mouse cDNA library from transgenic mouse transformed with mutant SOD (G39A) protein. Nat. Neurosci. (1097-6256) 2006 Only protein-protein interactions imex curation Chromogranin-mediated secretion of mutant superoxide dismutase proteins linked to amyotrophic lateral sclerosis. Chromogranin-mediated secretion of mutant superoxide dismutase proteins linked to amyotrophic lateral sclerosis. Nat. Neurosci. (1097-6256) 2006 imex curation Urushitani M., Sik A., Sakurai T., Nukina N., Takahashi R., Julien JP. BioCreative - Critical Assessment of Information Extraction systems in Biology Synapse - Interactions of proteins with an established role in the presynapse. The gly93ala, ala4val,gly37arg and gly85arg SOD1 mutations have been entered using sequence as stated in UniProt ID= P00441, as gly94ala, ala5val,gly38arg and gly86arg; assuming the N-terminal methionine was not included in the numbering used in this publication. julien@crchul.ulaval.ca 24-JAN-2007: Contacted by JYOTI. Mouse cDNA library from transgenic mouse transformed with mutant SOD (G39A) protein. Only protein-protein interactions mouse Mus musculus Mouse anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Urushitani M., Sik A., Sakurai T., Nukina N., Takahashi R., Julien JP. BioCreative - Critical Assessment of Information Extraction systems in Biology Cells from spinal neurons of transgenic (expressing SOD1 gly37arg) or wild type mice were used in this assay. julien@crchul.ulaval.ca Accepted 2006-SEP-13 by JYOTI. 24-JAN-2007: Contacted by JYOTI. Mouse cDNA library from transgenic mouse transformed with mutant SOD (G39A) protein. Nat. Neurosci. (1097-6256) 2006 Only protein-protein interactions imex curation tgon1_mouse Trans-Golgi network integral membrane protein 1 TGN38A Tgoln1 Ttgn1 protein protein mouse Mus musculus Mouse MRFQVALLLLSVAVARALPSVYKRDADSGDSQNPPNQPSKQSSTPLPSSNQVKTTRPTDGQGQKSDKKDQDKTTLAAVSSKAESGPRTAATDHSLGDSRRQPEKTDAELNETARPLSPVNPKLEKSDQSSTEDSGKPTGGNSGKPTGGDSGKPTEAGSNKATEDDSGKSTKVDLDKPTSKISPDTETSKTDKVQPTEKGQKPTLTSKTESGETLAGDSDFSLKPEKGDKSSEPTEDVETKEIEEGDTEPEEGSPLEEENEKVPGPSSSENQEGTLTDSMKNEKDDLYKDSSGNTSAESSHFFAYLVTAAVLVAVLYIAYHNKRKIIAFALEGKRSKVTRRPKASDYQRLNLKL 95C340C2F4A21EB3 scg1_mouse Secretogranin-1 Secretogranin I Chromogranin-B Chgb Scg1 Scg-1 protein protein mouse Mus musculus Mouse MQPAMLLGLLGAAALAAVSSAPVDNRDHNEEMVTRCIIEVLSNALSKSSVPTITPECRQVLKKSGKEVKGEEKGENQNSKFEVRLLRDPADASGTRWASSREDAGAPVEDSQGQTKVGNEKWTEGGGHSREGVDDQESLRPSNQQASKEAKIYHSEERVGKEREKEEGKIYPMGEHREDAGEEKKHIEDSGEKPNTFSNKRSEASAKKKDESVARADAHSMELEEKTHSREQSSQESGEETRRQEKPQELTDQDQSQEESQEGEEGEEGEEGEEGEEDSASEVTKRRPRHHHGRSGSNKSSYEGHPLSEERRPSPKESKEADVATVRLGEKRSHHLAHYRASEEEPEYGEESRSYRGLQYRGRGSEEDRAPRPRSEESQEREYKRNHPDSELESTANRHGEETEEERSYEGANGRQHRGRGREPGAHSALDTREEKRLLDEGHYPVRESPIDTAKRYPQSKWQEQEKNYLNYGEEGDQGRWWQQEEQLGPEESREEVRFPDRQYEPYPITEKRKRLGALFNPYFDPLQWKNSDFEKRGNPDDSFLEDEGEDRNGVTLTEKNSFPEYNYDWWERRPFSEDVNWGYEKRSFARAPQLDLKRQYDGVAELDQLLHYRKKADEFPDFYDSEEQMGPHQEANDEKARADQRVLTAEEKKELENLAAMDLELQKIAEKFSQRG C73291E781E4F9B9 stx1a_mouse Syntaxin-1A Stx1a Neuron-specific antigen HPC-1 protein protein mouse Mus musculus Mouse MKDRTQELRTAKDSDDDDDVTVTVDRDRFMDEFFEQVEEIRGFIDKIAENVEEVKRKHSAILASPNPDEKTKEELEELMSDIKKTANKVRSKLKSIEQSIEQEEGLNRSSADLRIRKTQHSTLSRKFVEVMSEYNATQSDYRERCKGRIQRQLEITGRTTTSEELEDMLESGNPAIFASGIIMDSSISKQALSEIETRHSEIIKLETSIRELHDMFMDMAMLVESQGEMIDRIEYNVEHAVDYVERAVSDTKKAVKYQSKARRKKIMIIICCVILGIIIASTIGGIFG DIP protein O35526 original sequence version: 122 DIP protein O35526 original sequence version: 124 DIP protein O35526 original sequence version: 139 8F8255F2EEF886CA cmga_mouse Chromogranin-A Chga protein protein mouse Mus musculus Mouse MRSTAVLALLLCAGQVFALPVNSPMTKGDTKVMKCVLEVISDSLSKPSPMPVSPECLETLQGDERILSILRHQNLLKELQDLALQGAKERAQQPLKQQQPPKQQQQQQQQQQQEQQHSSFEDELSEVFENQSPDAKHRDAAAEVPSRDTMEKRKDSDKGQQDGFEATTEGPRPQAFPEPNQESPMMGDSESPGEDTATNTQSPTSLPSQEHVDPQATGDSERGLSAQQQARKAKQEEKEEEEEEEAVAREKAGPEEVPTAASSSHFHAGYKAIQKDDGQSDSQAVDGDGKTEASEALPSEGKGELEHSQQEEDGEEAMVGTPQGLFPQGGKGRELEHKQEEEEEEEERLSREWEDKRWSRMDQLAKELTAEKRLEGEDDPDRSMKLSFRTRAYGFRDPGPQLRRGWRPSSREDSVEARSDFEEKKEEEGSANRRAEDQELESLSAIEAELEKVAHQLQALRRG 1AB3C5FF433C39E4 sodc_human Superoxide dismutase [Cu-Zn] Superoxide dismutase 1 SOD1 protein protein human Homo sapiens Human MATKAVCVLKGDGPVQGIINFEQKESNGPVKVWGSIKGLTEGLHGFHVHEFGDNTAGCTSAGPHFNPLSRKHGGPKDEERHVGDLGNVTADKDGVADVSIEDSVISLSGDHCIIGRTLVVHEKADDLGKGGNEESTKTGNAGSRLACGVIGIAQ DIP protein P00441 original sequence version: 194 DIP protein P00441 original sequence version: 189 DIP protein P00441 original sequence version: 186 DIP protein P00441 original sequence version: 147 DIP protein P00441 original sequence version: 148 DIP protein P00441 original sequence version: 145 DIP protein P00441 original sequence version: 169 25CA38DA8D564483 sod1-cgb-1 2 9 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait P00441:p.Gly94Ala mutation mutation certain certain sequence position certain certain certain sequence position certain G A Thu Jul 07 12:18:11 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was gly94ala Wed May 02 14:40:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly94ala' Tue Jun 30 15:56:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly94Ala' physical association physical association Interaction of CgA and CgB with mutant SOD in cultured cell lines. The authors state that a 76 amino acid region of the CgB protein interacted with the mutant SOD1 bait, but do not specify its coordinates. cgb-sod1-2 2 12 unspecified role unspecified role prey prey P00441:p.Gly94Ala mutation mutation certain certain sequence position certain certain certain sequence position certain G A Wed May 02 17:49:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly94ala' Tue Jun 30 20:08:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly94Ala' 9 unspecified role unspecified role bait bait physical association physical association Interaction of CgA and CgB with mutant SOD in cultured cell lines. sod1-cgb-2 3 12 unspecified role unspecified role bait bait P00441:p.Gly94Ala mutation mutation certain certain sequence position certain certain certain sequence position certain G A Wed May 02 18:11:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly94ala' Tue Jun 30 18:00:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly94Ala' n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P00441:p.Gly86Arg mutation mutation certain certain sequence position certain certain certain sequence position certain G R Wed May 02 14:40:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly86arg' Tue Jun 30 17:45:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly86Arg' P00441:p.Ala5Val mutation mutation certain certain sequence position certain certain certain sequence position certain A V Wed May 02 15:21:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala5val' Tue Jun 30 19:25:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala5Val' 9 unspecified role unspecified role prey prey c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Selective interactions of chromogranins with mutant SOD1 species but not with wild-type SOD1. Figure 1a sod1-cga-1 3 11 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role bait bait P00441:p.Gly94Ala mutation mutation certain certain sequence position certain certain certain sequence position certain G A Wed May 02 15:21:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly94ala' Tue Jun 30 17:20:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly94Ala' n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range P00441:p.Gly86Arg mutation mutation certain certain sequence position certain certain certain sequence position certain G R Wed May 02 18:11:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly86arg' Tue Jun 30 19:58:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly86Arg' P00441:p.Ala5Val mutation mutation certain certain sequence position certain certain certain sequence position certain A V Wed May 02 18:39:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala5val' Tue Jun 30 18:05:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala5Val' physical association physical association Selective interactions of chromogranins with mutant SOD1 species but not with wild-type SOD1. Figure 1b sod1-cgb-3 3 9 unspecified role unspecified role prey prey c terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 12 unspecified role unspecified role bait bait n-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association Selective interactions of chromogranins with mutant SOD1 species but not with wild-type SOD1. Figure 1c sod1-cgb-4 1 12 unspecified role unspecified role neutral component neutral component P00441:p.Gly86Arg mutation mutation certain certain sequence position certain certain certain sequence position certain G R Wed May 02 14:54:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly86arg' Tue Jun 30 19:05:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly86Arg' P00441:p.Gly94Ala mutation mutation certain certain sequence position certain certain certain sequence position certain G A Thu Jul 07 12:16:55 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was gly94ala Fri Jul 22 11:15:29 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gly93ala' Wed May 02 15:27:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly94ala' Tue Jun 30 18:30:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly94Ala' 9 unspecified role unspecified role neutral component neutral component c terminal egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization Interaction of CgA and CgB with mutant SOD in cultured cell lines. Figure 1j A total colocalisation of mutant SOD and CgB proteins was observed in roughly 10 percent of transfected cells. sod1-cgb-cga 5 11 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component P00441:p.Gly38Arg mutation mutation certain certain sequence position certain certain certain sequence position certain G R Wed May 02 18:44:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly38arg' Tue Jun 30 19:10:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly38Arg' 9 unspecified role unspecified role neutral component neutral component colocalization colocalization We also detected partial colocalisation of mutant SOD1 with CgA and CgB in irregular and large vesicular struuctures of spinal neurons from G37R transgenic mice. Figure 2c Partial colocalisation of mutant SOD1 with CgA and CgB was detected in in irregular and large vesicular struuctures of spinal neurons from G37R transgenic mice sod1-tgn38a 7 12 unspecified role unspecified role prey prey P00441:p.Gly38Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain G R Wed May 02 19:11:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly38arg' Tue Jun 30 19:45:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly38Arg' P00441:p.Gly94Ala mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain G A Wed May 02 20:31:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly94ala' Tue Jun 30 16:43:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly94Ala' 8 unspecified role unspecified role bait bait physical association physical association Western analysis of the immunoprecipitates demonstrated that both G37R and G93A SOD1 co-precipitated with TNG38 Figure 1a Interaction between mutated forms of SOD1 with TN38 protein was much greater than with SOD1 wild type and TN38 protein. sod1-cgb-cga-1 6 11 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component P00441:p.Gly38Arg mutation mutation certain certain sequence position certain certain certain sequence position certain G R Wed May 02 20:17:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly38arg' Tue Jun 30 17:21:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly38Arg' 9 unspecified role unspecified role neutral component neutral component physical association physical association Western blotting revealed that mutant SOD1 had a distribution pattern similar to chromogranins, the trans golgi marker adaptin and the SNARE protein syntaxin-1. Figure 3b Could not idetify adaptin protein. cga-sod1 7 12 unspecified role unspecified role prey prey P00441:p.Gly94Ala mutation mutation certain certain sequence position certain certain certain sequence position certain G A Wed May 02 19:05:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly94ala' Tue Jun 30 16:42:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly94Ala' P00441:p.Gly38Arg mutation mutation certain certain sequence position certain certain certain sequence position certain G R Wed May 02 19:10:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly38arg' Tue Jun 30 17:44:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly38Arg' 11 unspecified role unspecified role bait bait physical association physical association Pull down showing CgA and CgB interacted with mutant SOD1 Figure 3d cgb-sod1-1 7 9 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey P00441:p.Gly38Arg mutation mutation certain certain sequence position certain certain certain sequence position certain G R Wed May 02 16:10:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly38arg' Tue Jun 30 16:03:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly38Arg' P00441:p.Gly94Ala mutation mutation certain certain sequence position certain certain certain sequence position certain G A Wed May 02 15:22:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly94ala' Tue Jun 30 16:10:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly94Ala' physical association physical association Pull down showing CgA and CgB interacted with mutant SOD1 Figure 3d sod1-cgb-cga-2 4 11 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component P00441:p.Gly38Arg mutation mutation certain certain sequence position certain certain certain sequence position certain G R Wed May 02 14:51:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly38arg' Tue Jun 30 17:50:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly38Arg' colocalization colocalization No significant colocalisation with wild type (endogenous) SOD1 and chromogranins was observed in this study. Double immunohistochemistry using secondary antibodies conjugated with different gold particles (5nm and 10nm)provided frequent detection of cluster complexes comprising SOD1 and chromogranins. Figure 4