IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Histone demethylation by a family of JmjC domain-containing proteins. Histone demethylation by a family of JmjC domain-containing proteins. Nature (0028-0836) 2006 imex curation Tsukada Y., Fang J., Erdjument-Bromage H., Warren ME., Borchers CH., Tempst P., Zhang Y. BioCreative - Critical Assessment of Information Extraction systems in Biology Chromatin - Epigenetic interactions resulting in chromatin modulation yi_zhang@med.unc.edu 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro enzymatic study enzymatic study western blot western blot Immuno blot Immuno blot Tsukada Y., Fang J., Erdjument-Bromage H., Warren ME., Borchers CH., Tempst P., Zhang Y. BioCreative - Critical Assessment of Information Extraction systems in Biology yi_zhang@med.unc.edu Accepted 2006-SEP-14 by DAVET. Nature (0028-0836) 2006 Only protein-protein interactions imex curation Histone demethylation by a family of JmjC domain-containing proteins. Histone demethylation by a family of JmjC domain-containing proteins. Nature (0028-0836) 2006 imex curation Tsukada Y., Fang J., Erdjument-Bromage H., Warren ME., Borchers CH., Tempst P., Zhang Y. BioCreative - Critical Assessment of Information Extraction systems in Biology Chromatin - Epigenetic interactions resulting in chromatin modulation yi_zhang@med.unc.edu 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro enzymatic study enzymatic study ms participant Identification by mass spectrometry ms participant Tsukada Y., Fang J., Erdjument-Bromage H., Warren ME., Borchers CH., Tempst P., Zhang Y. yi_zhang@med.unc.edu Accepted 2006-SEP-14 by DAVET. Nature (0028-0836) 2006 kdm2a_human Lysine-specific demethylase 2A CXXC8 [Histone-H3]-lysine-36 demethylase 1A F-box/LRR-repeat protein 11 F-box and leucine-rich repeat protein 11 F-box protein FBL7 F-box protein Lilina CXXC-type zinc finger protein 8 KDM2A FBL7 KIAA1004 JHDM1A FBXL11 JmjC domain-containing histone demethylation protein 1A FBL11 protein protein human Homo sapiens Human MEPEEERIRYSQRLRGTMRRRYEDDGISDDEIEGKRTFDLEEKLHTNKYNANFVTFMEGKDFNVEYIQRGGLRDPLIFKNSDGLGIKMPDPDFTVNDVKMCVGSRRMVDVMDVNTQKGIEMTMAQWTRYYETPEEEREKLYNVISLEFSHTRLENMVQRPSTVDFIDWVDNMWPRHLKESQTESTNAILEMQYPKVQKYCLMSVRGCYTDFHVDFGGTSVWYHIHQGGKVFWLIPPTAHNLELYENWLLSGKQGDIFLGDRVSDCQRIELKQGYTFVIPSGWIHAVYTPTDTLVFGGNFLHSFNIPMQLKIYNIEDRTRVPNKFRYPFYYEMCWYVLERYVYCITNRSHLTKEFQKESLSMDLELNGLESGNGDEEAVDREPRRLSSRRSVLTSPVANGVNLDYDGLGKTCRSLPSLKKTLAGDSSSDCSRGSHNGQVWDPQCAPRKDRQVHLTHFELEGLRCLVDKLESLPLHKKCVPTGIEDEDALIADVKILLEELANSDPKLALTGVPIVQWPKRDKLKFPTRPKVRVPTIPITKPHTMKPAPRLTPVRPAAASPIVSGARRRRVRCRKCKACVQGECGVCHYCRDMKKFGGPGRMKQSCVLRQCLAPRLPHSVTCSLCGEVDQNEETQDFEKKLMECCICNEIVHPGCLQMDGEGLLNEELPNCWECPKCYQEDSSEKAQKRKMEESDEEAVQAKVLRPLRSCDEPLTPPPHSPTSMLQLIHDPVSPRGMVTRSSPGAGPSDHHSASRDERFKRRQLLRLQATERTMVREKENNPSGKKELSEVEKAKIRGSYLTVTLQRPTKELHGTSIVPKLQAITASSANLRHSPRVLVQHCPARTPQRGDEEGLGGEEEEEEEEEEEDDSAEEGGAARLNGRGSWAQDGDESWMQREVWMSVFRYLSRRELCECMRVCKTWYKWCCDKRLWTKIDLSRCKAIVPQALSGIIKRQPVSLDLSWTNISKKQLTWLVNRLPGLKDLLLAGCSWSAVSALSTSSCPLLRTLDLRWAVGIKDPQIRDLLTPPADKPGQDNRSKLRNMTDFRLAGLDITDATLRLIIRHMPLLSRLDLSHCSHLTDQSSNLLTAVGSSTRYSLTELNMAGCNKLTDQTLIYLRRIANVTLIDLRGCKQITRKACEHFISDLSINSLYCLSDEKLIQKIS DIP protein Q9Y2K7 original sequence version: 89 88620A363A5C5842 h3_peptide_31-41 consensus H3 peptide AA 31- 41 peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) STGGVKKPHRY An amino acid sequence which is not represented in UniProt Region of the peptide corresponding to the AA 31-41. Similar to P84244 Similar to P84243 379F4328633322C8 fbxlii-h3 1 4 enzyme target enzyme target substrate substrate unspecified role unspecified role region n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction Histone demethylation activity checked. Lysine 6 in the peptide corresponds to Lysine 36 in the conserved histone H3 N-terminal tail. 3C, 3D, S6 We analysed its site specificity using histone substrates radiolabelled at all known methylated sites in histones H3 (K4, K9, K27, K36, K79) and H4 (K20, R3). Of the seven substrates, only H3-K36 methylated by Set2 was a substrate for JHDM1A Thus, we conclude that JHDM1A is an H3-K36-specific demethylase. fbxlii-h3-1 2 4 enzyme target enzyme target substrate substrate unspecified role unspecified role lys6 n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 3 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction Histone demethylation activity checked. Lys6 in synthetic peptide corresponds to Lys36 in consensus H3 N-terminal tail. 3E We analysed its site specificity using histone substrates radiolabelled at all known methylated sites in histones H3 (K4, K9, K27, K36, K79) and H4 (K20, R3). Of the seven substrates, only H3-K36 methylated by Set2 was a substrate for JHDM1A Thus, we conclude that JHDM1A is an H3-K36-specific demethylase.