IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Release of the soluble transferrin receptor is directly regulated by binding of its ligand ferritransferrin. Release of the soluble transferrin receptor is directly regulated by binding of its ligand ferritransferrin. J. Biol. Chem. (0021-9258) 2006 imex curation Dassler K., Zydek M., Wandzik K., Kaup M., Fuchs H. BioCreative - Critical Assessment of Information Extraction systems in Biology hendrik.fuchs@charite.de 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro facs fluorescence-activated cell sorting FACS Flow cytometry facs elisa enzyme linked immunosorbent assay ELISA ELISA elisa Dassler K., Zydek M., Wandzik K., Kaup M., Fuchs H. BioCreative - Critical Assessment of Information Extraction systems in Biology hendrik.fuchs@charite.de Accepted 2006-SEP-15 by DAVET. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation trfe_human Serotransferrin Siderophilin Beta-1 metal-binding globulin TF PRO1400 protein protein human Homo sapiens Human MRLAVGALLVCAVLGLCLAVPDKTVRWCAVSEHEATKCQSFRDHMKSVIPSDGPSVACVKKASYLDCIRAIAANEADAVTLDAGLVYDAYLAPNNLKPVVAEFYGSKEDPQTFYYAVAVVKKDSGFQMNQLRGKKSCHTGLGRSAGWNIPIGLLYCDLPEPRKPLEKAVANFFSGSCAPCADGTDFPQLCQLCPGCGCSTLNQYFGYSGAFKCLKDGAGDVAFVKHSTIFENLANKADRDQYELLCLDNTRKPVDEYKDCHLAQVPSHTVVARSMGGKEDLIWELLNQAQEHFGKDKSKEFQLFSSPHGKDLLFKDSAHGFLKVPPRMDAKMYLGYEYVTAIRNLREGTCPEAPTDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAIAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP DIP protein P02787 original sequence version: 123 DIP protein P02787 original sequence version: 144 DIP protein P02787 original sequence version: 156 DIP protein P02787 original sequence version: 165 DIP protein P02787 original sequence version: 161 DIP protein P02787 original sequence version: 170 9A73B90D8C5671E9 tfr1_human Transferrin receptor protein 1 TFRC T9 p90 protein protein human Homo sapiens Human MMDQARSAFSNLFGGEPLSYTRFSLARQVDGDNSHVEMKLAVDEEENADNNTKANVTKPKRCSGSICYGTIAVIVFFLIGFMIGYLGYCKGVEPKTECERLAGTESPVREEPGEDFPAARRLYWDDLKRKLSEKLDSTDFTGTIKLLNENSYVPREAGSQKDENLALYVENQFREFKLSKVWRDQHFVKIQVKDSAQNSVIIVDKNGRLVYLVENPGGYVAYSKAATVTGKLVHANFGTKKDFEDLYTPVNGSIVIVRAGKITFAEKVANAESLNAIGVLIYMDQTKFPIVNAELSFFGHAHLGTGDPYTPGFPSFNHTQFPPSRSSGLPNIPVQTISRAAAEKLFGNMEGDCPSDWKTDSTCRMVTSESKNVKLTVSNVLKEIKILNIFGVIKGFVEPDHYVVVGAQRDAWGPGAAKSGVGTALLLKLAQMFSDMVLKDGFQPSRSIIFASWSAGDFGSVGATEWLEGYLSSLHLKAFTYINLDKAVLGTSNFKVSASPLLYTLIEKTMQNVKHPVTGQFLYQDSNWASKVEKLTLDNAAFPFLAYSGIPAVSFCFCEDTDYPYLGTTMDTYKELIERIPELNKVARAAAEVAGQFVIKLTHDVELNLDYERYNSQLLSFVRDLNQYRADIKEMGLSLQWLYSARGDFFRATSRLTTDFGNAEKTDRFVMKKLNDRVMRVEYHFLSPYVSPKESPFRHVFWGSGSHTLPALLENLKLRKQNNGAFNETLFRNQLALATWTIQGAANALSGDVWDIDNEF DIP protein P02786 original sequence version: 191 DIP protein P02786 original sequence version: 117 DIP protein P02786 original sequence version: 139 DIP protein P02786 original sequence version: 146 DIP protein P02786 original sequence version: 137 DIP protein P02786 original sequence version: 150 DIP protein P02786 original sequence version: 160 DIP protein P02786 original sequence version: 190 C886F14000D90154 tfrc-tf 1 2 unspecified role unspecified role neutral component neutral component 3 unspecified role unspecified role neutral component neutral component P02786:p.Arg646Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R K Wed May 02 19:11:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg646lys' Tue Jun 30 15:46:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg646Lys' P02786:p.Gly647Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G A Wed May 02 15:01:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly647ala' Tue Jun 30 19:18:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly647Ala' human-hl_60 Homo sapiens acute myeloid leukemia cell line hl_60 human acute myeloid leukemia cell line physical association physical association R646K slightly reduced the interaction and G647A strongly reduced interaction. 2 The transferrin was conjugated to iron in these studies. To demonstrate that the mutants indeed exhibit different binding affinities for transferrin, the cells were incubated with ferri-Tf-FITC and analyzed by flow cytometry.