IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. J. Biol. Chem. (0021-9258) 2006 imex curation McGuire JR., Rong J., Li SH., Li XJ. Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu 24-JAN-2007: Contacted by JYOTI. rat brain cDNA library Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined McGuire JR., Rong J., Li SH., Li XJ. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu Accepted 2006-SEP-8 by DAVET. 24-JAN-2007: Contacted by JYOTI. rat brain cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. J. Biol. Chem. (0021-9258) 2006 imex curation McGuire JR., Rong J., Li SH., Li XJ. Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu 24-JAN-2007: Contacted by JYOTI. rat brain cDNA library Only protein-protein interactions mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined McGuire JR., Rong J., Li SH., Li XJ. BioCreative - Critical Assessment of Information Extraction systems in Biology Hypothalamic tissue was used for HAP1 immunoprecipitation. xiaoli@genetics.emory.edu Accepted 2006-SEP-8 by DAVET. 24-JAN-2007: Contacted by JYOTI. rat brain cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. J. Biol. Chem. (0021-9258) 2006 imex curation McGuire JR., Rong J., Li SH., Li XJ. Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu 24-JAN-2007: Contacted by JYOTI. rat brain cDNA library Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence McGuire JR., Rong J., Li SH., Li XJ. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu Accepted 2006-SEP-8 by DAVET. rat brain cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. J. Biol. Chem. (0021-9258) 2006 imex curation McGuire JR., Rong J., Li SH., Li XJ. Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu 24-JAN-2007: Contacted by JYOTI. rat brain cDNA library Only protein-protein interactions rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells fluorescence imaging fluorescence microscopy fluorescence imaging predetermined predetermined participant predetermined McGuire JR., Rong J., Li SH., Li XJ. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu Accepted 2006-SEP-8 by DAVET. 24-JAN-2007: Contacted by JYOTI. rat brain cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. J. Biol. Chem. (0021-9258) 2006 imex curation McGuire JR., Rong J., Li SH., Li XJ. Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu 24-JAN-2007: Contacted by JYOTI. rat brain cDNA library Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined McGuire JR., Rong J., Li SH., Li XJ. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu Accepted 2006-SEP-8 by DAVET. 24-JAN-2007: Contacted by JYOTI. rat brain cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. J. Biol. Chem. (0021-9258) 2006 imex curation McGuire JR., Rong J., Li SH., Li XJ. Huntington's - Publications describing interactions involved in Huntington's disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu 24-JAN-2007: Contacted by JYOTI. rat brain cDNA library Only protein-protein interactions rat-pc12 Rattus norvegicus PC12 cells pc12 Rat adrenal pheochromocytoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot McGuire JR., Rong J., Li SH., Li XJ. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu Accepted 2006-SEP-8 by DAVET. 24-JAN-2007: Contacted by JYOTI. rat brain cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation o35668-2 Huntingtin-associated protein 1 Short Hap1 protein protein mouse Mus musculus Mouse MRPKEQVQSGAGDGTGSGDPAAGTPTTQPAVGPAPEPSAEPKPAPAQGTGSGQKSGSRTKTGSFCRSMIIGDSDAPWTRYVFQGPYGPRATGLGTGKAEGIWKTPAAYIGRRPGVSGPERAAFIRELQEALCPNPPPTKKITEDDVKVMLYLLEEKERDLNTAARIGQSLVKQNSVLMEENNKLETMLGSAREEILHLRKQVNLRDDLLQLYSDSDDDDDEEDEEDEEEGEEEEREGQRDQDQQHDHPYGAPKPHPKAETAHRCPQLETLQQKLRLLEEENDHLREEASHLDNLEDEEQMLILECVEQFSEASQQMAELSEVLVLRLEGYERQQKEITQLQAEITKLQQRCQSYGAQTEKLQQMLASEKGIHSESLRAGSYMQDYGSRPRDRQEDGKSHRQRSSMPAGSVTHYGYSVPLDALPSFPETLAEELRTSLRKFITDPAYFMERRDTHCREGRKKEQRAMPPPPAQDLKPPEDFEAPEELVPEEELGAIEEVGTAEDGQAEENEQASEETEAWEEVEPEVDETTRMNVVVSALEASGLGPSHLDMKYVLQQLSNWQDAHSKRQQKQKVVPKGECSRRGHPPASGTSFRSSTI Contains a phosphothreonine at position 598. 744EA32CB65A92F5 b2gv74_rat Kinesin light chain Klc2 Klc2_predicted rCG_48462 protein protein rat Rattus norvegicus (Rat) Rat MATMVLPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLASHEAGEAEPGSQERCLLLRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKLQRSEQAVAQLEEEKQHLLFMSQIRKLDEDASPNEEKGDVPKDSLDDLFPNEDEQSPAPSPGGGDVAAQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGPDDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGENKPIWMHAEEREESKDKRRDSAPYGEYGSWYKACKVDSPTVNTTLRSLGALYRRQGKLEAAHTLEDCASRSRKQGLDPASQTKVVELLKDGSGGRGDRRGSRDVAGGAGPRSESDLEESGPAAEWSGDGSGSLRRSGSFGKLRDALRRSSEMLVRKLQGGGPQEPPNSRMKRASSLNFLNKSVEEPVQPGGTGLSDSRTLSSSSMDLSRRSSLVG DIP protein B2GV74 original sequence version: 22 9198A0A0043DE3B5 p54256-1 Huntingtin-associated protein 1 Long Hap1 protein protein rat Rattus norvegicus (Rat) Rat MRPKDQVQSSAGDGTGSGDPATGTPTTQPAADPAPEPSAEPKPAPAQGTGSGQKSGSRTKTGGSFCRSRIRGDSDAPWTRYIFQGPYGPRATGLGTGRAEGIWKTPAAYIGRRPGVSGPERAAFIRELQEALCPNPLPRKKITEDDIKVMLYLLEEKERDLNTAARIGQSLVKQNSVLMEENNKLETMLGSAREEILHLRKQVNLRDDLLQLYSDSDDDEEDEEDEEEEEGEEEEREGQRDQDQQHDHPYGAPKPPPKAETLHHCPQLEALKQKLKLLEEENDHLREEASHLDNLEDKEQMLILECVEQFSEASQQMAELSEVLVLRLEGYERQQKEITQLQAEITKLQQRCQSYGAQTEKLQQQLASEKGVHPESLRAGSHMQDYGSRPRERQEDGKSHRQRSSMPAGSVTHYGYSVPLDALPSFPETLAEELRTSLRKFITDPAYFMERCDTRCREERKKEQGTMPPPPVQDLKPPEDFEAPEELVPEEELGAIEEVGTAEDGPAEETEQASEETEAWEEVEPEVDEATRMNVVVSALEASGLGPSHLDMKYVLQQLSNWQDAHSKRQQKQKVVPKDSPAPQQQTNMGGGIVEQQPIVPTQDSQRLEEDRATHSPSAREEEGPSGAT C23C5CB57520B574 klc1_mouse Kinesin light chain 1 Klc1 Kns2 protein protein mouse Mus musculus Mouse MYDNMSTMVYIKEEKLENVTQDEIISKTKQVIQGLEALKNEHNSILQSLLETLKCLKKDDESNLVEEKSSMIRKSLEMLELGLSEAQVMMALSNHLNAVESEKQNVRAQVRRLCQENQWLRDELANTQQKLQKSEQSVAQLEEEKKHLEFMNQLKKYDDDISPSEDKDSDSSKEPLDDLFPNDEDEPGQGIQHSDSSAAAARQGYEIPARLRTLHNLVIQYASQGRYEVAVPSCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAANLLNDALAIREKTLGRDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKDHPDVAKQLNNLALLCQNQGKYEEVEYYYQRALGIYQTKLGPDRTPNVAKTKNNLASCYLKQGKFKQAETLYKEILTRAHEAEFGSVDDENKPIWMHAEEREECKGKQKDGSAFGEYGGWYKACKVDSPTVTTTLKNLGALYRRQGKFEAAETLEEAAMRSRKQGLDNVHKQRVAEVLNDPESMEKRRSRESLNMDVVKYESGPDGGEEA DIP protein O88447 original sequence version: 83 550F23AB36049C03 klc2_mouse Kinesin light chain 2 Klc2 protein protein mouse Mus musculus Mouse MATMVLPREEKLSQDEIVLGTKAVIQGLETLRGEHRALLAPLASHEAGEAEPGSQERCLLLRRSLEAIELGLGEAQVILALSSHLGAVESEKQKLRAQVRRLVQENQWLREELAGTQQKLQRSEQAVAQLEEEKQHLLFMSQIRKLDEMLPQEEKGDVPKDSLDDLFPNEDEQSPAPSPGGGDVAAQHGGYEIPARLRTLHNLVIQYASQGRYEVAVPLCKQALEDLEKTSGHDHPDVATMLNILALVYRDQNKYKDAAHLLNDALAIREKTLGKDHPAVAATLNNLAVLYGKRGKYKEAEPLCKRALEIREKVLGKFHPDVAKQLSNLALLCQNQGKAEEVEYYYRRALEIYATRLGPDDPNVAKTKNNLASCYLKQGKYQDAETLYKEILTRAHEKEFGSVNGENKPIWMHAEEREESKDKRRDRRPMEYGSWYKACKVDSPTVNTTLRTLGALYRPEGKLEAAHTLEDCASRSRKQGLDPASQTKVVELLKDGSGRGHRRGSRDVAGPQSESDLEESGPAAEWSGDGSGSLRRSGSFGKLRDALRRSSEMLVRKLQGGGPQEPNSRMKRASSLNFLNKSVEEPVQPGGRVFLTAAL DIP protein O88448 original sequence version: 114 DIP protein O88448 original sequence version: 80 A5E6F9081609E1CE kif5c_rat Kinesin heavy chain isoform 5C Kinesin heavy chain neuron-specific 2 Kif5c Nkhc2 protein protein rat Rattus norvegicus (Rat) Rat MADPAECSIKVMCRFRPLNEAEILRGDKFIPKFKGEETVVIGQGKPYVFDRVLPPNTTQEQVYNACAKQIVKDVLEGYNGTIFAYGQTSSGKTHTMEGKLHDPQLMGIIPRIAHDIFDHIYSMDENLEFHIKVSYFEIYLDKIRDLLDVSKTNLAVHEDKNRVPYVKGCTERFVSSPEEVMDVIDEGKANRHVAVTNMNEHSSRSHSIFLINIKQENVETEKKLSGKLYLVDLAGSEKV C96C5FFE4F8E1D7E p54256-2 Huntingtin-associated protein 1 Short Hap1 protein protein rat Rattus norvegicus (Rat) Rat MRPKDQVQSSAGDGTGSGDPATGTPTTQPAADPAPEPSAEPKPAPAQGTGSGQKSGSRTKTGGSFCRSRIRGDSDAPWTRYIFQGPYGPRATGLGTGRAEGIWKTPAAYIGRRPGVSGPERAAFIRELQEALCPNPLPRKKITEDDIKVMLYLLEEKERDLNTAARIGQSLVKQNSVLMEENNKLETMLGSAREEILHLRKQVNLRDDLLQLYSDSDDDEEDEEDEEEEEGEEEEREGQRDQDQQHDHPYGAPKPPPKAETLHHCPQLEALKQKLKLLEEENDHLREEASHLDNLEDKEQMLILECVEQFSEASQQMAELSEVLVLRLEGYERQQKEITQLQAEITKLQQRCQSYGAQTEKLQQQLASEKGVHPESLRAGSHMQDYGSRPRERQEDGKSHRQRSSMPAGSVTHYGYSVPLDALPSFPETLAEELRTSLRKFITDPAYFMERCDTRCREERKKEQGTMPPPPVQDLKPPEDFEAPEELVPEEELGAIEEVGTAEDGPAEETEQASEETEAWEEVEPEVDEATRMNVVVSALEASGLGPSHLDMKYVLQQLSNWQDAHSKRQQKQKVVPKGECSRRGHPPASGTSYRSSTL Contains a phosphothreonine at position 598. DA2949D7676C9434 hap1_mouse Huntingtin-associated protein 1 Hap1 protein protein mouse Mus musculus Mouse MRPKEQVQSGAGDGTGSGDPAAGTPTTQPAVGPAPEPSAEPKPAPAQGTGSGQKSGSRTKTGSFCRSMIIGDSDAPWTRYVFQGPYGPRATGLGTGKAEGIWKTPAAYIGRRPGVSGPERAAFIRELQEALCPNPPPTKKITEDDVKVMLYLLEEKERDLNTAARIGQSLVKQNSVLMEENNKLETMLGSAREEILHLRKQVNLRDDLLQLYSDSDDDDDEEDEEDEEEGEEEEREGQRDQDQQHDHPYGAPKPHPKAETAHRCPQLETLQQKLRLLEEENDHLREEASHLDNLEDEEQMLILECVEQFSEASQQMAELSEVLVLRLEGYERQQKEITQLQAEITKLQQRCQSYGAQTEKLQQMLASEKGIHSESLRAGSYMQDYGSRPRDRQEDGKSHRQRSSMPAGSVTHYGYSVPLDALPSFPETLAEELRTSLRKFITDPAYFMERRDTHCREGRKKEQRAMPPPPAQDLKPPEDFEAPEELVPEEELGAIEEVGTAEDGQAEENEQASEETEAWEEVEPEVDETTRMNVVVSALEASGLGPSHLDMKYVLQQLSNWQDAHSKRQQKQKVVPKDSPTPQQQTNMGGGILEQQPRVPTQDSQRLEEDRATHSPSAREEEGPSGAT DIP protein O35668 original sequence version: 96 DIP protein O35668 original sequence version: 77 AF516F61D6AB7CCB hap1-klc2 3 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role bait bait physical association physical association The coiled-coil domains of HAP1 do not appear to mediate the binding of HAP1 to KLC, as HAP1 fragments that contained coiled-coil domains but lacked C-terminal amino acids failed to yield a positive interaction 1 hap1-klc2-1 5 7 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-994324]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 599-599, sequence length 598 [EBI-994324]445-599 8 unspecified role unspecified role prey prey physical association physical association Yeast two-hybrid analysis revealed that a C-terminal fragment of HAP1 present in both rodent isoforms binds kinesin light chain-2 (KLC-2, Fig. 1), a subtype of KLC that is highly homologous to kinesin light chain-1 (KLC-1) with 71.1% amino acid identity 1B hap1-klc2-2 1 8 unspecified role unspecified role prey prey human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 7 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-994342]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 599-599, sequence length 598 [EBI-994342]445-599 ecoli Escherichia coli (strain K12) physical association physical association A GST fusion protein consisting of a HAP1 fragment (160-599 amino acids), but not 160-445 amino acids, precipitated KLC-2, confirming the yeast two-hybrid results 1C hap1-klc2-3 2 10 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey association association 1D Only when HAP1 was present did HAP1 immunoprecipitation coprecipitate kinesin (Fig. 1D), indicating that KLC associates with HAP1 in vivo. The antibody used reacts with both KLC-1 and KLC-2 in the brain. KLC-1 is the major form of KLC in the hypothalamus and was coprecipitated by the antibody to HAP1. hap1-khc 4 13 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component colocalization colocalization The colocalisation was observed in the presence of NGF treatment. KHC and HAP1-A were clearly colocalized in the same neurite tips 2B klc2-hap1 6 8 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey association association 2C As expected, immunoprecipitation of either HAP1-A or HAP1-B from PC12 cells pulled down both HAP1-A and HAP1-B. Because PC12 cells express more HAP1-B than HAP1-A, the HAP1-B immunoprecipitation yielded a much greater amount of HAP1-B than HAP1-A. hap1-khc-1 4 12 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization The colocalisation was observed in the presence of NGF treatment. KHC and HAP1-A were clearly colocalized in the same neurite tips 3