IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Organization of calcium channel beta1a subunits in triad junctions in skeletal muscle. Organization of calcium channel beta1a subunits in triad junctions in skeletal muscle. J. Biol. Chem. (0021-9258) 2006 imex curation Leuranguer V., Papadopoulos S., Beam KG. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Leuranguer V., Papadopoulos S., Beam KG. BioCreative - Critical Assessment of Information Extraction systems in Biology xiaoli@genetics.emory.edu Accepted 2006-SEP-18 by DAVET. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation cacb1_rabit Voltage-dependent L-type calcium channel subunit beta-1 Calcium channel voltage-dependent subunit beta 1 CACNB1 CACNLB1 protein protein rabit Oryctolagus cuniculus (Rabbit) Rabbit MVQKTSMSRGPYPPSQEIPMEVFDPSPQGKYSKRKGRFKRSDGSTSSDTTSNSFVRQGSAESYTSRPSDSDVSLEEDREALRKEAERQALAQLEKAKTKPVAFAVRTNVGYNPSPGDEVPVEGVAITFEPKDFLHIKEKYNNDWWIGRLVKEGCEVGFIPSPVKLDSLRLLQEQKLRQSRLSSSKSGDNSSSSLGDVVTGTRRPTPPASGNEMTNLAFELEPLDLEEDEAELGEQSGSAKTSVSSVTTPPPHGTRIPFFKKTEHVPPYDVVPSMRPIILVGPSLKGYEVTDMMQKALFDFLKHLFDGRISITRVTADISLAKRSVLNNPSKHIIIERSNTRSSLAEVQSEIERIFELARTLQLVALDADTINHPAQLSKTSLAPIIVYIKITSPKVLQRLIKSRGKSQSKHLNVQIAASEKLAQCPPEMFDIILDENQLEDACEHLAEYLEAYWKATHPPSSTPPNPLLNRTMATAALAASPAPVSNLQVQVLTSLRRNLSFWGGLETSQRGGGAVPQQQEHAM DIP protein P19517 original sequence version: 117 629420640A7457CC cacnb1-cacnb1 1 2 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 2 unspecified role unspecified role fluorescence donor fluorescence donor region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Expression was in dysgenic beta null myotubes. 5 in the cytoplasm: the 6-7% intramolecular FRET efficiency of the doubly labeled constructs (CFP-beta1a-YFP or YFP-beta1a-CFP) expressed in dysgenic myotubes indicates that the N and C termini of cytoplasmic beta1a subunits are likely separated by <10 nm. Fourth, within junctions: there was no measurable intermolecular FRET for 1:1 mixtures of CFP-beta1a + YFP-beta1a, beta1a-CFP + beta1a-YFP, or CFP-beta1a + beta1a-YFP expressed in beta-null myotubes. This result is consistent with the idea that then N and C termini of adjacent beta1a subunits are >10 nm apart, which would occur if they were oriented away from the center of tetrads.