IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Retention of mutant low density lipoprotein receptor in endoplasmic reticulum (ER) leads to ER stress. Retention of mutant low density lipoprotein receptor in endoplasmic reticulum (ER) leads to ER stress. J. Biol. Chem. (0021-9258) 2006 imex curation Sorensen S., Ranheim T., Bakken K.S., Leren T.P., Kulseth M.A. BioCreative - Critical Assessment of Information Extraction systems in Biology no mari.ann.kulseth@rikshospitalet.no 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sorensen S., Ranheim T., Bakken K.S., Leren T.P., Kulseth M.A. BioCreative - Critical Assessment of Information Extraction systems in Biology no The interactions between chaperones and folding polypeptides are transient in nature. To prevent the release of chaperones from the substrates, cross-linking was therefore performed by adding DSP before the cells were solubilized in lysis buffer. mari.ann.kulseth@rikshospitalet.no Accepted 2006-SEP-1 by DAVET. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Retention of mutant low density lipoprotein receptor in endoplasmic reticulum (ER) leads to ER stress. Retention of mutant low density lipoprotein receptor in endoplasmic reticulum (ER) leads to ER stress. J. Biol. Chem. (0021-9258) 2006 imex curation Sorensen S., Ranheim T., Bakken K.S., Leren T.P., Kulseth M.A. BioCreative - Critical Assessment of Information Extraction systems in Biology no mari.ann.kulseth@rikshospitalet.no 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sorensen S., Ranheim T., Bakken K.S., Leren T.P., Kulseth M.A. BioCreative - Critical Assessment of Information Extraction systems in Biology no mari.ann.kulseth@rikshospitalet.no Accepted 2006-SEP-1 by DAVET. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation g3idt6_crigr Protein disulfide-isomerase A4 Endoplasmic reticulum resident protein 72 I79_021872 CgPICR_019838 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MKLRKAWLLVLLLALTQLLAAASATDAHEDASDTENAIEDEEEEEEDEEEDDLEVKEENGVLVLNDANFDNFVADKDTVLLEFYAPWCGHCKQFAPEYEKIASTLKDNDPPIAVAKIDATSASMLASRFDVSGYPTIKILKKGQAVDYEGSRTQEEIVAKVREVSQPDWTPPPEVTLLLTKDNFDDVVNNADIILVEFYAPWCGHCKKLAPEYEKAAKELSKHSPPIPLAKVDATEQTDLAKRFDVSGYPTLKIFRKGRPFDYNGPREKYGIVSYMIEQSGPPSKEILTLKQVQEFLKDGDDVVIIGVFQGDSDPGYLQYQDAANNLREDYRFHHTFNTEIAKFLKVSMGKLVLMQPEKFQSKYEPRSNVMDVQGSTEPSAIKDYVVKHALPLVGHRKTSNDAKRYTKRPLVVVYYSVDFSFDYRTATQFWRNKVLEVAKDFPEYTFAIADEEDYATEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDADTLREFVTAFKKGKLKPVIKSQPIPKNNKGPVKVVVGKTFDAIVMDPKKDVLIEFYAPWCGHCKQLEPIYTNLGKKYKGQKDLVIAKMDATANDITNDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIDEHATKKSRTKEEL 9E7F89FAFCFBAB0D bip_crigr Endoplasmic reticulum chaperone BiP HSPA5 78 kDa glucose-regulated protein Binding-immunoglobulin protein GRP78 Heat shock protein 70 family protein 5 Heat shock protein family A member 5 Immunoglobulin heavy chain-binding protein I79_019946 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MKFPMVAAALLLLCAVRAEEEDKKEDVGTVVGIDLGTTYSCVGVFKNGRVEIIANDQGNRITPSYVAFTPEGERLIGDAAKNQLTSNPENTVFDAKRLIGRTWNDPSVQQDIKFLPFKVVEKKTKPYIQVDIGGGQTKTFAPEEISAMVLTKMKETAEAYLGKKVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVMRIINEPTAAAIAYGLDKREGEKNILVFDLGGGTFDVSLLTIDNGVFEVVATNGDTHLGGEDFDQRVMEHFIKLYKKKTGKDVRKDNRAVQKLRREVEKAKRALSSQHQARIEIESFFEGEDFSETLTRAKFEELNMDLFRSTMKPVQKVLEDSDLKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTSEKDEL 25CF665F59113A49 g3hqm6_crigr I79_013131 protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MRVLWVLGLCCVLLTFGFVRADDEVDVDGTVEEDLGKSREGSRTDDEVVQREEEAIQLDGLNASQIRELREKSEKFAFQAEVNRMMKLIINSLYKNKEIFLRELISNASDALDKIRLISLTDENALAGNEELTVKIKCDKEKNLLHVTDTGVGMTREELVKNLGTIAKSGTSEFLNKMTEAQEDGQSTSELIGQFGVGFYSAFLVADKVIVTSKHNNDTQHIWESDSNEFSVIADPRGNTLGRGTTITLVLKEEASDYLELDTIKNLVRKYSQFINFPIYVWSSKTETVEEPLEEDEAAKEEKEESDDEAAVEEEEEEKKPKTKKVEKTVWDWELMNDIKPIWQRPSKEVEEDEYKAFYKSFSKESDDPMAYIHFTAEGEVTFKSILFVPTSAPRGLFDEYGSKKSDYIKLYVRRVFITDDFHDMMPKYLNFVKGVVDSDDLPLNVSRETLQQHKLLKVIRKKLVRKTLDMIKKIADEKYNDTFWKEFGTNIKLGVIEDHSNRTRLAKLLRFQSSHHSTDITSLDQYVERMKEKQDKIYFMAGSSRKEAESSPFVERLLKKGYEVIYLTEPVDEYCIQALPEFDGKRFQNVAKEGVKFDESEKTKENREATEKEFEPLLNWMKDKALKDKIEKAVVSQRLTESPCALVASQYGWSGNMERIMKAQAYQTGKDISTNYYASQKKTFEINPRHPLIRDMLRRVKEDEDDKTVLDLAVVLFETATLRSGYLLPDTKAYGDRIERMLRLSLNIDPEAQVEEEPEEEPEDTTEDTEQDEEEEVDAGTEEEEEEEQETAKESTAEKDEL CFB30C02A1347B9B ldlr_human Low-density lipoprotein receptor LDLR protein protein human Homo sapiens Human MGPWGWKLRWTVALLLAAAGTAVGDRCERNEFQCQDGKCISYKWVCDGSAECQDGSDESQETCLSVTCKSGDFSCGGRVNRCIPQFWRCDGQVDCDNGSDEQGCPPKTCSQDEFRCHDGKCISRQFVCDSDRDCLDGSDEASCPVLTCGPASFQCNSSTCIPQLWACDNDPDCEDGSDEWPQRCRGLYVFQGDSSPCSAFEFHCLSGECIHSSWRCDGGPDCKDKSDEENCAVATCRPDEFQCSDGNCIHGSRQCDREYDCKDMSDEVGCVNVTLCEGPNKFKCHSGECITLDKVCNMARDCRDWSDEPIKECGTNECLDNNGGCSHVCNDLKIGYECLCPDGFQLVAQRRCEDIDECQDPDTCSQLCVNLEGGYKCQCEEGFQLDPHTKACKAVGSIAYLFFTNRHEVRKMTLDRSEYTSLIPNLRNVVALDTEVASNRIYWSDLSQRMICSTQLDRAHGVSSYDTVISRDIQAPDGLAVDWIHSNIYWTDSVLGTVSVADTKGVKRKTLFRENGSKPRAIVVDPVHGFMYWTDWGTPAKIKKGGLNGVDIYSLVTENIQWPNGITLDLLSGRLYWVDSKLHSISSIDVNGGNRKTILEDEKRLAHPFSLAVFEDKVFWTDIINEAIFSANRLTGSDVNLLAENLLSPEDMVLFHNLTQPRGVNWCERTTLSNGGCQYLCLPAPQINPHSPKFTCACPDGMLLARDMRSCLTEAEAAVATQETSTVRLKVSSTAVRTQHTTTRPVPDTSRLPGATPGLTTVEIVTMSHQALGDVAGRGNEKKPSSVRALSIVLPIVLLVFLCLGVFLLWKNWRLKNINSINFDNPVYQKTTEDEVHICHNQDGYSYPSRQMVSLEDDVA DIP protein P01130 original sequence version: 115 DIP protein P01130 original sequence version: 122 DIP protein P01130 original sequence version: 140 DIP protein P01130 original sequence version: 173 A4C28E9B8BADAD5E q8k3h8_crigr protein protein crigr Cricetulus griseus (Chinese hamster) Cricetulus barabensis griseus Chinese hamster MEGKWLLCLLLVLGTVAVQAHDGHDDDMIDIEDDLDDVIEEVEDSKSKSDSSTPPSPKVTYKAPVPTGEVYFADSFDRGSLSGWILSKAKKDDTDDEIAKYDGKWEVDEMKDTKLPGDKGLVLMSRAKHHAISAKLNKPFLFDTKPLIVQYEVNFQNGIECGGAYVKLLSKTSELNLDQFHDKTPYTIMFGPDKCGEDYKLHFIFRHKNPKTGVYEEKHAKRPDTDLKIYFTDKKTHLYTLILNPDNSFEILVDQYVVNSGNLLNDMTPAVNPSREIEDPEDQKXEDWDERPKIPDPDAVKPDDWDEDAPGKIPDEEATKPEGWLDDEPEYIPDPDADKPEDWDEDMDGEWEAPQIANPKCESAPGCGVWQRPLIDNPNYKGKWKPPMIDNPNYQGVWKPRKIPNPDFFEDLEPFKMTPFSAIGLELWSMTSDIFFDNFIISGDRRVVDDWANDGWGLKKAADGAAEPGVVGHMLEAAEERPWLWVVYILTVALPVFLVILFCCSGKKQSNAMEYKKTDAAQPDVKEDEGKEEEKNKGDEEEEEEKLEEKQKSDAEEDGGTGSQDEEDRKPTAEEDEILNRSPRNRKPRRE 6F68D4D5522E7DE3 ldlr-hspa5 2 4 unspecified role unspecified role prey prey crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 6 unspecified role unspecified role bait bait P01130:p.Gly565Val mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain G V Wed May 02 15:52:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly565val' Tue Jun 30 19:47:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly565Val' crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association The LDLR is presumed to be human as the antibody used (61099) was stated to be specific for human LDLR on the provider's (Progen Biotechnik) website. 2 C Grp78 co-immunoprecipitated with the G544V mutant LDLR from cells treated with or without crosslinker. Grp78 co-immunoprecipitated with the G544V mutant LDLR from cells treated with or without crosslinker. ldlr-hsp90b1 1 5 unspecified role unspecified role prey prey crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 6 unspecified role unspecified role bait bait P01130:p.Gly565Val mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain G V Wed May 02 19:51:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly565val' Tue Jun 30 20:06:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly565Val' crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association The LDLR is presumed to be human as the antibody used (61099) was stated to be specific for human LDLR on the provider's (Progen Biotechnik) website. 2 C The chaperones Grp94, ERp72, and calnexin were only co-immunoprecipitated with G544V mutant LDLR in cells pretreated with cross-linker. The chaperones Grp94, ERp72, and calnexin were only co-immunoprecipitated with G544V mutant LDLR in cells pretreated with cross-linker. These results indicate that the ER-resident chaperones Grp78, Grp94, ERp72, and calnexin are involved in the retention of the G544V mutant LDLR in ER. The chaperone proteins Grp94, ERp72 and calnexin only interact with the G544V mutant LDLR and not the wild-type. ldlr-pdia4 1 6 unspecified role unspecified role bait bait P01130:p.Gly565Val mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain G V Wed May 02 14:54:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly565val' Tue Jun 30 18:37:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly565Val' crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 3 unspecified role unspecified role prey prey crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association The LDLR is presumed to be human as the antibody used (61099) was stated to be specific for human LDLR on the provider's (Progen Biotechnik) website. 2 C The chaperones Grp94, ERp72, and calnexin were only co-immunoprecipitated with G544V mutant LDLR in cells pretreated with cross-linker. The chaperones Grp94, ERp72, and calnexin were only co-immunoprecipitated with G544V mutant LDLR in cells pretreated with cross-linker. These results indicate that the ER-resident chaperones Grp78, Grp94, ERp72, and calnexin are involved in the retention of the G544V mutant LDLR in ER. The chaperone proteins Grp94, ERp72 and calnexin only interact with the G544V mutant LDLR and not the wild-type. ldlr-q8k3h8 1 7 unspecified role unspecified role prey prey crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 6 unspecified role unspecified role bait bait P01130:p.Gly565Val mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain G V Wed May 02 16:04:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly565val' Tue Jun 30 17:21:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly565Val' crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association The LDLR is presumed to be human as the antibody used (61099) was stated to be specific for human LDLR on the provider's (Progen Biotechnik) website. 2 C The chaperones Grp94, ERp72, and calnexin were only co-immunoprecipitated with G544V mutant LDLR in cells pretreated with cross-linker. The chaperones Grp94, ERp72, and calnexin were only co-immunoprecipitated with G544V mutant LDLR in cells pretreated with cross-linker. These results indicate that the ER-resident chaperones Grp78, Grp94, ERp72, and calnexin are involved in the retention of the G544V mutant LDLR in ER. The chaperone proteins Grp94, ERp72 and calnexin only interact with the G544V mutant LDLR and not the wild-type.