IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Leu309 plays a critical role in the encapsulation of substrate protein into the internal cavity of GroEL. Leu309 plays a critical role in the encapsulation of substrate protein into the internal cavity of GroEL. J. Biol. Chem. (0021-9258) 2006 imex curation Koike-Takeshita A., Shimamura T., Yokoyama K., Yoshida M., Taguchi H. BioCreative - Critical Assessment of Information Extraction systems in Biology A number of residues in both proteins were replaced by cysteine, proteins then cross-linked when dithiothreotol was removed from the reaction mixture. myoshida@res.titech.ac.jp 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Koike-Takeshita A., Shimamura T., Yokoyama K., Yoshida M., Taguchi H. BioCreative - Critical Assessment of Information Extraction systems in Biology A number of residues in both proteins were replaced by cysteine, proteins then cross-linked when dithiothreotol was removed from the reaction mixture. myoshida@res.titech.ac.jp Accepted 2006-AUG-4 by JYOTI. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Leu309 plays a critical role in the encapsulation of substrate protein into the internal cavity of GroEL. Leu309 plays a critical role in the encapsulation of substrate protein into the internal cavity of GroEL. J. Biol. Chem. (0021-9258) 2006 imex curation Koike-Takeshita A., Shimamura T., Yokoyama K., Yoshida M., Taguchi H. BioCreative - Critical Assessment of Information Extraction systems in Biology A number of residues in both proteins were replaced by cysteine, proteins then cross-linked when dithiothreotol was removed from the reaction mixture. myoshida@res.titech.ac.jp 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Koike-Takeshita A., Shimamura T., Yokoyama K., Yoshida M., Taguchi H. BioCreative - Critical Assessment of Information Extraction systems in Biology A number of residues in both proteins were replaced by cysteine, proteins then cross-linked when dithiothreotol was removed from the reaction mixture. myoshida@res.titech.ac.jp Accepted 2006-AUG-4 by JYOTI. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Leu309 plays a critical role in the encapsulation of substrate protein into the internal cavity of GroEL. Leu309 plays a critical role in the encapsulation of substrate protein into the internal cavity of GroEL. J. Biol. Chem. (0021-9258) 2006 imex curation Koike-Takeshita A., Shimamura T., Yokoyama K., Yoshida M., Taguchi H. BioCreative - Critical Assessment of Information Extraction systems in Biology A number of residues in both proteins were replaced by cysteine, proteins then cross-linked when dithiothreotol was removed from the reaction mixture. myoshida@res.titech.ac.jp 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink predetermined predetermined participant predetermined Koike-Takeshita A., Shimamura T., Yokoyama K., Yoshida M., Taguchi H. BioCreative - Critical Assessment of Information Extraction systems in Biology A number of residues in both proteins were replaced by cysteine, proteins then cross-linked when dithiothreotol was removed from the reaction mixture. myoshida@res.titech.ac.jp Accepted 2006-AUG-4 by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation ch60_ecoli Chaperonin GroEL groEL 60 kDa chaperonin Chaperonin-60 GroEL protein groL mopA JW4103 b4143 protein protein ecoli Escherichia coli (strain K12) MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITEGLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGKLIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESPFILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIIDGVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAPLRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM CD3A0FB505F74AD1 CD3A0FB505F74AD1 mdh_ecoli Malate dehydrogenase mdh b3236 JW3205 protein protein ecoli Escherichia coli (strain K12) MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAVDLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLVQQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAELKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKAGGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVEERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK DIP protein P61889 original sequence version: 65 17741A3B5AD068BA ch10_ecoli Co-chaperonin GroES groES 10 kDa chaperonin Chaperonin-10 groS mopB JW4102 b4142 protein protein ecoli Escherichia coli (strain K12) MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVGNGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA 76829E09B11217EF 76829E09B11217EF glpe_ecoli Thiosulfate sulfurtransferase GlpE glpE b3425 JW3388 protein protein ecoli Escherichia coli (strain K12) MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLTNDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPAEVAYGA 18D5B461C2A8EF19 grol-gros-1 3 6 unspecified role unspecified role neutral component neutral component P0A6F9:p.Thr28Cys mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T C Wed May 02 16:36:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr28cys' Tue Jun 30 19:40:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr28Cys' P0A6F9:p.Gly29Cys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain G C Wed May 02 16:43:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly29cys' Tue Jun 30 19:26:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly29Cys' P0A6F9:p.Ser30Cys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain S C Wed May 02 17:49:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser30cys' Wed Jun 13 17:26:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser30cys' Tue Jun 30 18:45:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser30Cys' ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component P0A6F5:p.Met307Cys mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain M C Wed May 02 18:06:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met307cys' Tue Jun 30 18:01:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met307Cys' P0A6F5:p.Glu308Cys mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E C Wed May 02 19:01:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu308cys' Tue Jun 30 17:55:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu308Cys' P0A6F5:p.Leu309Cys mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L C Wed May 02 14:38:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu309cys' Tue Jun 30 16:27:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu309Cys' P0A6F5:p.Glu310Cys mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E C Wed May 02 19:36:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu310cys' Tue Jun 30 17:10:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu310Cys' P0A6F5:p.Glu308Cys mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain E C Wed May 02 16:20:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu308cys' Wed May 02 19:28:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu308cys' Tue Jun 30 17:07:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu308Cys' Fri Sep 18 22:18:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu308Cys' ecoli Escherichia coli (strain K12) 11 18 18 12 direct interaction direct interaction Interaction requires presence of ATP To investigate whether or not the GXXLE region is in contact with GroES in the E. coli GroEL GroES complex, we conducted a series of cross-linking experiments using mutants of E. coli GroEL and GroES. 2 grol-mdh-1 2 5 unspecified role unspecified role neutral component neutral component region cy3 label cy3 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component P0A6F5:p.Leu309Lys mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L K Wed May 02 18:04:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu309lys' Tue Jun 30 17:07:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu309Lys' ecoli Escherichia coli (strain K12) direct interaction direct interaction we sought to analyze further the properties of GroEL(L309K). The mutant protein was mixed with denaturedMDHCy3 and analyzed using gel filtration HPLC with elution in a buffer containing ATP (Fig. 5A). 5 grol-gros-2 2 4 unspecified role unspecified role neutral component neutral component P0A6F5:p.Leu309Lys mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L K Wed May 02 18:38:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu309lys' Tue Jun 30 17:15:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu309Lys' ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component region cy3 label cy3 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction In the absence of denatured MDH, GroEL(L309K) formed a complex with GroESCy3 (Fig. 5C). 5C grol-glpe-1 1 4 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction Interaction requires presence of ATP In a control experiment, 1 mol of free wild type GroEL or GroEL(L309K) (without GroES, nucleotide and BeFx) bound 2 mol of rhodanese (lanes 1 and 2). 6 grol-glpe-2 1 6 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction Interaction stabilised by the presence of beryllium fluoride, a phosphate analogue The GroEL(L309K) complex, formed in the presence of ADP and BeFx, had an apparent composition of 1:1:2 (GroEL(L309K) GroES rhodanese) 6