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  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>IntAct</shortLabel>
        <fullName>European Bioinformatics Institute</fullName>
        <alias type="synonym" typeAc="MI:1041">IntAct</alias>
        <alias type="synonym" typeAc="MI:1041">intact</alias>
      </names>
      <bibref>
        <xref>
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      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/</attribute>
        <attribute name="search-url" nameAc="MI:0615">http://www.ebi.ac.uk/intact/query/${ac}</attribute>
        <attribute name="id-validation-regexp" nameAc="MI:0628">EBI-[0-9]+|IA:[0-9]+</attribute>
        <attribute name="definition">INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions.</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact</attribute>
        <attribute name="postaladdress">European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom</attribute>
        <attribute name="url" nameAc="MI:0614">http://www.ebi.ac.uk/intact/</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Crystal structure of AqpZ tetramer reveals two distinct Arg-189 conformations associated with water permeation through the narrowest constriction of the water-conducting channel.</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="16239219" refType="primary-reference" refTypeAc="MI:0358"/>
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          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Crystal structure of AqpZ tetramer reveals two distinct Arg-189 conformations associated with water permeation through the narrowest constriction of the water-conducting channel.</attribute>
            <attribute name="journal" nameAc="MI:0885">J. Biol. Chem. (0021-9258)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2006</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Jiang J., Daniels BV., Fu D.</attribute>
            <attribute name="dataset" nameAc="MI:0875">BioCreative - Critical Assessment of Information Extraction systems in Biology</attribute>
            <attribute name="contact-email" nameAc="MI:0634">dax@bnl.gov</attribute>
            <attribute name="author-announcement">14-DEC-2006: Contacted by JYOTI.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="imex" dbAc="MI:0670" id="IM-14495" refType="imex-primary" refTypeAc="MI:0662"/>
          <secondaryRef db="pubmed" dbAc="MI:0446" id="16239219" refType="primary-reference" refTypeAc="MI:0358"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>x-ray diffraction</shortLabel>
            <fullName>x-ray crystallography</fullName>
            <alias type="go synonym" typeAc="MI:0303">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">X-ray</alias>
            <alias type="synonym" typeAc="MI:1041">x-ray diffraction</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0114" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1272" refType="identity" refTypeAc="MI:0356"/>
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        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
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          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Jiang J., Daniels BV., Fu D.</attribute>
          <attribute name="dataset" nameAc="MI:0875">BioCreative - Critical Assessment of Information Extraction systems in Biology</attribute>
          <attribute name="contact-email" nameAc="MI:0634">dax@bnl.gov</attribute>
          <attribute name="accepted">Accepted 2006-JUN-22 by DAVET.</attribute>
          <attribute name="author-announcement">14-DEC-2006: Contacted by JYOTI.</attribute>
          <attribute name="journal" nameAc="MI:0885">J. Biol. Chem. (0021-9258)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2006</attribute>
          <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
          <attribute name="imex curation" nameAc="MI:0959"/>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>aqpz_ecoli</shortLabel>
          <fullName>Aquaporin Z</fullName>
          <alias type="gene name" typeAc="MI:0301">aqpZ</alias>
          <alias type="gene name synonym" typeAc="MI:0302">bniP</alias>
          <alias type="locus name" typeAc="MI:0305">b0875</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Bacterial nodulin-like intrinsic protein</alias>
          <alias type="locus name" typeAc="MI:0305">JW0859</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="P60844" version="SP_45" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="AAC73962" version="SP_148" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="BAA35589" version="SP_148" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="P48838" version="SP_45" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="P75827" version="SP_45" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q47159" version="SP_45" refType="secondary-ac" refTypeAc="MI:0360"/>
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          <secondaryRef db="ensemblbacteria" id="AAC73962" version="SP_148" refType="transcript"/>
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          <secondaryRef db="ensemblbacteria" id="BAA35589" version="SP_148" refType="transcript"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005886" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005887" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006833" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0006970" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0009992" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0015250" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016021" version="SP_148"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042802" version="SP_148"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR000425" version="SP_148"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR022357" version="SP_148"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR023271" version="SP_148"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR023743" version="SP_148"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR034294" version="SP_148"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="1RC2" version="SP_148"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_415396.1"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2ABM" version="SP_148"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2O9D" version="SP_148"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2O9E" version="SP_148"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2O9F" version="SP_148"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="2O9G" version="SP_148"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3NK5" version="SP_148"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3NKA" version="SP_148"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3NKC" version="SP_148"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="WP_001298299.1"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-35499N"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="83333">
          <names>
            <shortLabel>ecoli</shortLabel>
            <fullName>Escherichia coli (strain K12)</fullName>
          </names>
        </organism>
        <sequence>MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFGLTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLIASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD</sequence>
        <attributeList>
          <attribute name="crc64">3BDE1A932D45CF14</attribute>
          <attribute name="crc64">3BDE1A932D45CF14</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="3" imexId="IM-14495-1">
        <names>
          <shortLabel>aqpz-1</shortLabel>
        </names>
        <xref>
          <primaryRef db="rcsb pdb" dbAc="MI:0460" id="2abm" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-957676" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-14495-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="4">
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>neutral component</shortLabel>
                  <fullName>neutral component</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0497" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-55" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="511693">
                <names>
                  <shortLabel>ecobb</shortLabel>
                  <fullName>Escherichia coli (strain B / BL21)</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
            <stoichiometry value="4"/>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="3d-resolution" nameAc="MI:0632">3.20�</attribute>
          <attribute name="3d-r-factors" nameAc="MI:0631">working 18.6%, free 24.2%</attribute>
          <attribute name="source-text">a 3.2 angstroms crystal structure of the tetrameric AqpZ</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
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