IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Regulation of sensory neuron-specific acid-sensing ion channel 3 by the adaptor protein Na+/H+ exchanger regulatory factor-1. Regulation of sensory neuron-specific acid-sensing ion channel 3 by the adaptor protein Na+/H+ exchanger regulatory factor-1. J. Biol. Chem. (0021-9258) 2006 imex curation Deval E., Friend V., Thirant C., Salinas M., Jodar M., Lazdunski M., Lingueglia E. BioCreative - Critical Assessment of Information Extraction systems in Biology lazdunski@ipmc.cnrs.fr 24-JAN-2007: Contacted by JYOTI. Adult rat dorsal root ganglia cDNA library Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Deval E., Friend V., Thirant C., Salinas M., Jodar M., Lazdunski M., Lingueglia E. BioCreative - Critical Assessment of Information Extraction systems in Biology lazdunski@ipmc.cnrs.fr Accepted 2006-SEP-8 by ORCHARD. 24-JAN-2007: Contacted by JYOTI. Adult rat dorsal root ganglia cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Regulation of sensory neuron-specific acid-sensing ion channel 3 by the adaptor protein Na+/H+ exchanger regulatory factor-1. Regulation of sensory neuron-specific acid-sensing ion channel 3 by the adaptor protein Na+/H+ exchanger regulatory factor-1. J. Biol. Chem. (0021-9258) 2006 imex curation Deval E., Friend V., Thirant C., Salinas M., Jodar M., Lazdunski M., Lingueglia E. BioCreative - Critical Assessment of Information Extraction systems in Biology lazdunski@ipmc.cnrs.fr 24-JAN-2007: Contacted by JYOTI. Adult rat dorsal root ganglia cDNA library Only protein-protein interactions rat-brain Rattus norvegicus brain brain Brain [cerebrum] fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Deval E., Friend V., Thirant C., Salinas M., Jodar M., Lazdunski M., Lingueglia E. BioCreative - Critical Assessment of Information Extraction systems in Biology lazdunski@ipmc.cnrs.fr Accepted 2006-SEP-8 by ORCHARD. 24-JAN-2007: Contacted by JYOTI. Adult rat dorsal root ganglia cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Regulation of sensory neuron-specific acid-sensing ion channel 3 by the adaptor protein Na+/H+ exchanger regulatory factor-1. Regulation of sensory neuron-specific acid-sensing ion channel 3 by the adaptor protein Na+/H+ exchanger regulatory factor-1. J. Biol. Chem. (0021-9258) 2006 imex curation Deval E., Friend V., Thirant C., Salinas M., Jodar M., Lazdunski M., Lingueglia E. BioCreative - Critical Assessment of Information Extraction systems in Biology lazdunski@ipmc.cnrs.fr 24-JAN-2007: Contacted by JYOTI. Adult rat dorsal root ganglia cDNA library Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Deval E., Friend V., Thirant C., Salinas M., Jodar M., Lazdunski M., Lingueglia E. BioCreative - Critical Assessment of Information Extraction systems in Biology lazdunski@ipmc.cnrs.fr Accepted 2006-SEP-8 by ORCHARD. Adult rat dorsal root ganglia cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Regulation of sensory neuron-specific acid-sensing ion channel 3 by the adaptor protein Na+/H+ exchanger regulatory factor-1. Regulation of sensory neuron-specific acid-sensing ion channel 3 by the adaptor protein Na+/H+ exchanger regulatory factor-1. J. Biol. Chem. (0021-9258) 2006 imex curation Deval E., Friend V., Thirant C., Salinas M., Jodar M., Lazdunski M., Lingueglia E. BioCreative - Critical Assessment of Information Extraction systems in Biology lazdunski@ipmc.cnrs.fr 24-JAN-2007: Contacted by JYOTI. Adult rat dorsal root ganglia cDNA library Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Deval E., Friend V., Thirant C., Salinas M., Jodar M., Lazdunski M., Lingueglia E. BioCreative - Critical Assessment of Information Extraction systems in Biology lazdunski@ipmc.cnrs.fr Accepted 2006-SEP-8 by ORCHARD. 24-JAN-2007: Contacted by JYOTI. Adult rat dorsal root ganglia cDNA library J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation nhrf1_rat Na(+)/H(+) exchange regulatory cofactor NHE-RF1 Nherf1 Ezrin-radixin-moesin-binding phosphoprotein 50 Regulatory cofactor of Na(+)/H(+) exchanger Sodium-hydrogen exchanger regulatory factor 1 Solute carrier family 9 isoform A3 regulatory factor 1 Slc9a3r1 Nherf protein protein rat Rattus norvegicus (Rat) Rat MSADAAAGEPLPRLCCLEKGPNGYGFHLHGEKGKVGQFIRLVEPGSPAEKSGLLAGDRLVEVNGENVEKETHQQVVSRIRAALNAVRLLVVDPETDEQLKKLGVPIREELLRAQEKSEHTEPPAAADTKKAGDQNEAEKSHLERCELRPRLCTMKKGPNGYGFNLHSDKSKPGQFIRAVDPDSPAEASGLRAQDRIVEVNGVCMEGKQHGDVVSAIKAGGDEAKLLVVDKETDEFFKKCRVTPSQEHLDGPLPEPFSNGEIQKENSREALVEPASESPRPALARSASSDTSEELNAQDSPKRHDSTEPSSTSSSSDPILDFNISLAVAKERAHQKRSSKRAPQMDWSKKNELFSNL C562AEF5870B8E08 nhrf2_rat Na(+)/H(+) exchange regulatory cofactor NHE-RF2 Tyrosine kinase activator protein 1 SRY-interacting protein 1 Solute carrier family 9 isoform A3 regulatory factor 2 NHE3 kinase A regulatory protein E3KARP Sodium-hydrogen exchanger regulatory factor 2 Slc9a3r2 Nherf2 protein protein rat Rattus norvegicus (Rat) Rat MAAPESLRPRLCRLVRGEQGYGFHLHGEKGRRGQFIRRVEPGSPAEAAALRAGDRLVEVNGVNVEGETHHQVVHRIKAVEGQTQLLVVDKETDEELCRRQLTCTEEMAHRGLPPAHNPWEPKPDWACSGSLGSDTGHKDVNGPPRELRPRLCHLRRGPQGYGFNLHSDKSRPGQYIRSVDPGSPASLSGLRAQDRLIEVNGQNVEGLRHAEVVARIKAQEDEARLLVVDPETDEHFKRLRVVPTEDHVEGPLPSPVTNGTSLAQLNGGSVCSSRSDLPGSEKDNEDGSAWKRDPFQESGLHLSPTAAEAKEKARATRVNKRAPQMDWNRKREIFSNF C4B8E6D0CCFDBB0E asic3_rat Acid-sensing ion channel 3 Asic3 Accn3 Amiloride-sensitive cation channel 3 Dorsal root ASIC Drasic protein protein rat Rattus norvegicus (Rat) Rat MKPRSGLEEAQRRQASDIRVFASSCTMHGLGHIFGPGGLTLRRGLWATAVLLSLAAFLYQVAERVRYYGEFHHKTTLDERESHQLTFPAVTLCNINPLRRSRLTPNDLHWAGTALLGLDPAEHAAYLRALGQPPAPPGFMPSPTFDMAQLYARAGHSLEDMLLDCRYRGQPCGPENFTVIFTRMGQCYTFNSGAHGAELLTTPKGGAGNGLEIMLDVQQEEYLPIWKDMEETPFEVGIRVQIHSQDEPPAIDQLGFGAAPGHQTFVSCQQQQLSFLPPPWGDCNTASLDPDDFDPEPSDPLGSPRPRPSPPYSLIGCRLACESRYVARKCGCRMMHMPGNSPVCSPQQYKDCASPALDAMLRKDTCVCPNPCATTRYAKELSMVRIPSRASARYLARKYNRSESYITENVLVLDIFFEALNYEAVEQKAAYEVSELLGDIGGQMGLFIGASLLTILEILDYLCEVFQDRVLGYFWNRRSAQKRSGNTLLQEELNGHRTHVPHLSLGPRPPTTPCAVTKTLSASHRTCYLVTRL DIP protein O35240 original sequence version: 94 294B57322C74B3DC ezri_cerae_protein Ezrin (p81) (Cytovillin) (Villin-2) protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt The protein was detected using in situ hybridisation using an anti-ezrin antibody. The source and prooduction details used in production of this assay are not provided in the corresponding paper. asic3-nherf_1 3 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association The yeast two-hybrid system was used to identify putative ASIC3-interacting proteins. A rat DRG cDNA library (18) was screened with the last 68 amino acids of rat ASIC3 as a bait. Among the isolated clones, one clone encoded a full-length NHERF-1 protein, also named EBP50 (ezrin-radixin- moesin-binding phosphoprotein of 50 kDa), and another encoded a partial NHERF-2 protein, also identified as E3KARP (Na /H exchanger 3 kinase A-regulatory protein), TKA-1 (tyrosine kinase activator- 1), or SIP-1 (SRY-interacting protein-1). asic3-nherf_2 3 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait c terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association The yeast two-hybrid system was used to identify putative ASIC3-interacting proteins. A rat DRG cDNA library (18) was screened with the last 68 amino acids of rat ASIC3 as a bait. Among the isolated clones, one clone encoded a full-length NHERF-1 protein, also named EBP50 (ezrin-radixin- moesin-binding phosphoprotein of 50 kDa), and another encoded a partial NHERF-2 protein, also identified as E3KARP (Na /H exchanger 3 kinase A-regulatory protein), TKA-1 (tyrosine kinase activator- 1), or SIP-1 (SRY-interacting protein-1). asic3-nherf_1-1 1 5 unspecified role unspecified role bait bait n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region required to bind necessary binding region required to bind range range range range 7 unspecified role unspecified role prey prey c terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O35240:p.Ser523Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain S G Wed May 02 16:13:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser523gly' Tue Jun 30 18:44:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser523Gly' O35240:p.Ser523Asp mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain S D Wed May 02 18:08:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser523asp' Tue Jun 30 17:43:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser523Asp' physical association physical association PDZ-dependent interaction of NHERF-1 and NHERF-2 with ASIC3 in COS-7 cells. The coordiates of the deleted PDZ region for NHERF-1 are not given in the text. The coordinates for the PDZ region were taken from the UniProt entry Q9JJ19. Figure 1A Figure 2 phorbol 12-myristate 13-acetate (PMA) chelerythrine The authors note that "All of these data strongly suggest that PKC phosphorylation of serine 523 in the COOH-terminal region of ASIC3 positively modulates the interaction between ASIC3 and NHERF-1." asic3-nherf_2-1 1 6 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind range range range range n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 7 unspecified role unspecified role prey prey c terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association PDZ-dependent interaction of NHERF-1 and NHERF-2 with ASIC3 in COS-7 cells. The coordiates of the deleted PDZ region for NHERF-1 are not given in the text. The coordinates for the PDZ region were taken from the UniProt entry Q920G2. Figure 1 B asic3-nherf_1-2 2 5 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization Co-localization of ASIC3 and NHERF-1 Figure 4E asic3-nherf_1-3 4 5 unspecified role unspecified role neutral component neutral component n-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region required to bind necessary binding region required to bind range range range range 7 unspecified role unspecified role neutral component neutral component c terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization NHERF-1 co-localizes with ASIC3 in COS-7 cells and alters its cellular distribution. Figure 5F asic3-nherf_1-4 4 5 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component n-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization ASIC3 co-localizes with endogenous ezrin in the presence of NHERF-1. Figure 6 No UniProt accession number is available for Ezrin at this time.