IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Novel fluorescent prothrombin analogs as probes of staphylocoagulase-prothrombin interactions. Novel fluorescent prothrombin analogs as probes of staphylocoagulase-prothrombin interactions. J. Biol. Chem. (0021-9258) 2006 imex curation Panizzi P., Friedrich R., Fuentes-Prior P., Kroh HK., Briggs J., Tans G., Bode W., Bock PE. BioCreative - Critical Assessment of Information Extraction systems in Biology Fluorescence titrations of an array of ProT analogs with was carried out. paul.bock@vanderbilt.edu 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro competition binding competition binding predetermined predetermined participant predetermined Panizzi P., Friedrich R., Fuentes-Prior P., Kroh HK., Briggs J., Tans G., Bode W., Bock PE. BioCreative - Critical Assessment of Information Extraction systems in Biology Fluorescence titrations of an array of ProT analogs with was carried out. paul.bock@vanderbilt.edu Accepted 2006-SEP-8 by DAVET. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Novel fluorescent prothrombin analogs as probes of staphylocoagulase-prothrombin interactions. Novel fluorescent prothrombin analogs as probes of staphylocoagulase-prothrombin interactions. J. Biol. Chem. (0021-9258) 2006 imex curation Panizzi P., Friedrich R., Fuentes-Prior P., Kroh HK., Briggs J., Tans G., Bode W., Bock PE. BioCreative - Critical Assessment of Information Extraction systems in Biology Fluorescence titrations of an array of ProT analogs with was carried out. paul.bock@vanderbilt.edu 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Panizzi P., Friedrich R., Fuentes-Prior P., Kroh HK., Briggs J., Tans G., Bode W., Bock PE. BioCreative - Critical Assessment of Information Extraction systems in Biology Fluorescence titrations of an array of ProT analogs with was carried out. paul.bock@vanderbilt.edu Accepted 2006-SEP-8 by DAVET. 24-JAN-2007: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation Novel fluorescent prothrombin analogs as probes of staphylocoagulase-prothrombin interactions. Novel fluorescent prothrombin analogs as probes of staphylocoagulase-prothrombin interactions. J. Biol. Chem. (0021-9258) 2006 imex curation Panizzi P., Friedrich R., Fuentes-Prior P., Kroh HK., Briggs J., Tans G., Bode W., Bock PE. BioCreative - Critical Assessment of Information Extraction systems in Biology Fluorescence titrations of an array of ProT analogs with was carried out. paul.bock@vanderbilt.edu 24-JAN-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Panizzi P., Friedrich R., Fuentes-Prior P., Kroh HK., Briggs J., Tans G., Bode W., Bock PE. BioCreative - Critical Assessment of Information Extraction systems in Biology Fluorescence titrations of an array of ProT analogs with was carried out. paul.bock@vanderbilt.edu Accepted 2006-SEP-8 by DAVET. J. Biol. Chem. (0021-9258) 2006 Only protein-protein interactions imex curation q846v4_staau protein protein staau Staphylococcus aureus IVTKDYSKESRVNENSKYGTLISDWYLKGRLTSLESQFINALGILETYHYGEKEYKDAKDKLMTRILGEDQYLLERKKVQYEEYKKLYKKYKEENPTSKVKMKTFDQYTIEDLTMREYNELTESLKSAVKDFEKDVEIIENQHHDLKPFTDEMEEKATARVDDLANKAYSVYFAFVRDTQHKTEALELKAKVDLVLGDEDKPHRISNERIEKEMIKDLESIIEDFFIETGLNKPDNITSYDSSKHHYKNHSEGFEALVKETREAVTNANDSWKTKTVKKYGETVTKPPVVKEEKKVEEPQAPKVSEKVDVQETVGTEETTQPVAQPLVKIPQGTIYGETVKGPEYLTMENKTLQGEIVQGPDFPTMEQNRPSLSDNYTQPTTPNPILKGLEGSSSKLEIKPQGTESTLKGTQGESSDIEVKPQASETTEASHYPARPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETNAYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHGNGQVSYGARPTYKKPSKTNAYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHGNGQVSYGARPTQNKPSKTNAYNVTTHGNGQVSYGARPTQNKPSKTNAYNVTTHANGQVSYGARPTYKKPSETNAYNVTTHADGTATYGSRVTK 2D703F06BAFC3C13 thrb_human Prothrombin F2 Coagulation factor II protein protein human Homo sapiens Human MAHVRGLQLPGCLALAALCSLVHSQHVFLAPQQARSLLQRVRRANTFLEEVRKGNLERECVEETCSYEEAFEALESSTATDVFWAKYTACETARTPRDKLAACLEGNCAEGLGTNYRGHVNITRSGIECQLWRSRYPHKPEINSTTHPGADLQENFCRNPDSSTTGPWCYTTDPTVRRQECSIPVCGQDQVTVAMTPRSEGSSVNLSPPLEQCVPDRGQQYQGRLAVTTHGLPCLAWASAQAKALSKHQDFNSAVQLVENFCRNPDGDEEGVWCYVAGKPGDFGYCDLNYCEEAVEEETGDGLDEDSDRAIEGRTATSEYQTFFNPRTFGSGEADCGLRPLFEKKSLEDKTERELLESYIDGRIVEGSDAEIGMSPWQVMLFRKSPQELLCGASLISDRWVLTAAHCLLYPPWDKNFTENDLLVRIGKHSRTRYERNIEKISMLEKIYIHPRYNWRENLDRDIALMKLKKPVAFSDYIHPVCLPDRETAASLLQAGYKGRVTGWGNLKETWTANVGKGQPSVLQVVNLPIVERPVCKDSTRIRITDNMFCAGYKPDEGKRGDACEGDSGGPFVMKSPFNNRWYQMGIVSWGEGCDRDGKYGFYTHVFRLKKWIQKVIDQFGE DIP protein P00734 original sequence version: 122 DIP protein P00734 original sequence version: 127 DIP protein P00734 original sequence version: 129 8A25E1DA88208FCF sc-f2-3 3 5 unspecified role unspecified role neutral component neutral component human-plasma homo sapiens blood plasma plasma blood plasma 4 unspecified role unspecified role neutral component neutral component n terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecobb Escherichia coli (strain B / BL21) physical association physical association titrations of 50-285 nM labeled ProT indicated that SC-(1-325) bound ProT with a 0.84-1.00 stoichiometry for those that could be determined (ACR, AF350, BD, 4'F, 5F, OG, and TMR) 2, 3 Analogues of ProT were competed out by SC. sc-f2-4 1 5 unspecified role unspecified role bait bait human-plasma homo sapiens blood plasma plasma blood plasma 4 unspecified role unspecified role prey prey n terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Competitive binding of SC-(1�325) to native ProT and [OG]FPR-ProT. 6 Analogues of ProT were competed out by SC. sc-f2-5 2 5 self self unspecified role unspecified role human-plasma homo sapiens blood plasma plasma blood plasma 4 unspecified role unspecified role ancillary ancillary n terminal region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain cleavage cleavage reaction cleavage 4, 5 Increasing concentration of SC resulted in increased self cleavage of ProThrombin. Proteolytic cleavage of ProT in the presence of SC