UniProt UniProt http://www.uniprot.org Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B. Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B. J. Biol. Chem. (0021-9258) 2001 imex curation Sekiguchi T., Hirose E., Nakashima N., Ii M., Nishimoto T. Apoptosis - Interactions involving proteins with a function related to apoptosis BioCreative - Critical Assessment of Information Extraction systems in Biology sekigu@molbiol.med.kyushu-u.ac.jp 14-DEC-2006: Contacted by JYOTI. Human Burkitt's lymphoma cDNA library Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Sekiguchi T., Hirose E., Nakashima N., Ii M., Nishimoto T. Apoptosis - Interactions involving proteins with a function related to apoptosis sekigu@molbiol.med.kyushu-u.ac.jp Accepted 2006-AUG-24 by ORCHARD. 14-DEC-2006: Contacted by JYOTI. Human Burkitt's lymphoma cDNA library J. Biol. Chem. (0021-9258) 2001 Only protein-protein interactions imex curation Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B. Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B. J. Biol. Chem. (0021-9258) 2001 imex curation Sekiguchi T., Hirose E., Nakashima N., Ii M., Nishimoto T. Apoptosis - Interactions involving proteins with a function related to apoptosis BioCreative - Critical Assessment of Information Extraction systems in Biology sekigu@molbiol.med.kyushu-u.ac.jp 14-DEC-2006: Contacted by JYOTI. Human Burkitt's lymphoma cDNA library Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Sekiguchi T., Hirose E., Nakashima N., Ii M., Nishimoto T. Apoptosis - Interactions involving proteins with a function related to apoptosis sekigu@molbiol.med.kyushu-u.ac.jp Accepted 2006-AUG-24 by ORCHARD. 14-DEC-2006: Contacted by JYOTI. Human Burkitt's lymphoma cDNA library J. Biol. Chem. (0021-9258) 2001 Only protein-protein interactions imex curation Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B. Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B. J. Biol. Chem. (0021-9258) 2001 imex curation Sekiguchi T., Hirose E., Nakashima N., Ii M., Nishimoto T. Apoptosis - Interactions involving proteins with a function related to apoptosis BioCreative - Critical Assessment of Information Extraction systems in Biology sekigu@molbiol.med.kyushu-u.ac.jp 14-DEC-2006: Contacted by JYOTI. Human Burkitt's lymphoma cDNA library Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Sekiguchi T., Hirose E., Nakashima N., Ii M., Nishimoto T. Apoptosis - Interactions involving proteins with a function related to apoptosis sekigu@molbiol.med.kyushu-u.ac.jp Accepted 2006-AUG-24 by ORCHARD. 14-DEC-2006: Contacted by JYOTI. Human Burkitt's lymphoma cDNA library J. Biol. Chem. (0021-9258) 2001 Only protein-protein interactions imex curation Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B. Novel G proteins, Rag C and Rag D, interact with GTP-binding proteins, Rag A and Rag B. J. Biol. Chem. (0021-9258) 2001 imex curation Sekiguchi T., Hirose E., Nakashima N., Ii M., Nishimoto T. Apoptosis - Interactions involving proteins with a function related to apoptosis BioCreative - Critical Assessment of Information Extraction systems in Biology sekigu@molbiol.med.kyushu-u.ac.jp 14-DEC-2006: Contacted by JYOTI. Human Burkitt's lymphoma cDNA library Only protein-protein interactions mesau-bhk21 Mesocricetus auratus_BHK21 bhk-21 Cells from kidneys of five unsexed, one-day-old hamsters BHK-21/C13 cell anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sekiguchi T., Hirose E., Nakashima N., Ii M., Nishimoto T. Apoptosis - Interactions involving proteins with a function related to apoptosis Hamster BHK21 cells have been tzransfected with human RRAGA, RRAGB, RRAGC and RRAGD. sekigu@molbiol.med.kyushu-u.ac.jp Accepted 2006-AUG-24 by ORCHARD. 14-DEC-2006: Contacted by JYOTI. Human Burkitt's lymphoma cDNA library J. Biol. Chem. (0021-9258) 2001 Only protein-protein interactions imex curation rraga_human Ras-related GTP-binding protein A Adenovirus E3 14.7 kDa-interacting protein 1 FIP-1 RRAGA protein protein human Homo sapiens Human MPNTAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATIDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPDAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECACFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLRNFAQIIEADEVLLFERATFLVISHYQCKEQRDVHRFEKISNIIKQFKLSCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKLERVDGPKHSLLMR DIP protein Q7L523 original sequence version: 116 DIP protein Q7L523 original sequence version: 104 B0DA1FC8FA6B766A rragb_human Ras-related GTP-binding protein B RRAGB protein protein human Homo sapiens Human MEESDSEKTTEKENLGPRMDPPLGEPEGSLGWVLPNTAMKKKVLLMGKSGSGKTSMRSIIFANYIARDTRRLGATILDRIHSLQINSSLSTYSLVDSVGNTKTFDVEHSHVRFLGNLVLNLWDCGGQDTFMENYFTSQRDNIFRNVEVLIYVFDVESRELEKDMHYYQSCLEAILQNSPDAKIFCLVHKMDLVQEDQRDLIFKEREEDLRRLSRPLECSCFRTSIWDETLYKAWSSIVYQLIPNVQQLEMNLRNFAEIIEADEVLLFERATFLVISHYQCKEQRDAHRFEKISNIIKQFKLSCSKLAASFQSMEVRNSNFAAFIDIFTSNTYVMVVMSDPSIPSAATLINIRNARKHFEKLERVDGPKQCLLMR DIP protein Q5VZM2 original sequence version: 89 0D32F7D86E2A00DE rragd_human Ras-related GTP-binding protein D RRAGD protein protein human Homo sapiens Human MSQVLGKPQPQDEDDAEEEEEEDELVGLADYGDGPDSSDADPDSGTEEGVLDFSDPFSTEVKPRILLMGLRRSGKSSIQKVVFHKMSPNETLFLESTNKICREDVSNSSFVNFQIWDFPGQIDFFDPTFDYEMIFRGTGALIFVIDSQDDYMEALARLHLTVTRAYKVNTDINFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKIHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSTPVDMQTYELCCDMIDVVIDISCIYGLKEDGAGTPYDKESTAIIKLNNTTVLYLKEVTKFLALVCFVREESFERKGLIDYNFHCFRKAIHEVFEVRMKVVKSRKVQNRLQKKKRATPNGTPRVLL 96FF0854B11AA1BC rragc_human Ras-related GTP-binding protein C GTPase-interacting protein 2 TIB929 RRAGC protein protein human Homo sapiens Human MSLQYGAEETPLAGSYGAADSFPKDFGYGVEEEEEEAAAAGGGVGAGAGGGCGPGGADSSKPRILLMGLRRSGKSSIQKVVFHKMSPNETLFLESTNKIYKDDISNSSFVNFQIWDFPGQMDFFDPTFDYEMIFRGTGALIYVIDAQDDYMEALTRLHITVSKAYKVNPDMNFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKLHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSSPVDMQSYELCCDMIDVVIDVSCIYGLKEDGSGSAYDKESMAIIKLNNTTVLYLKEVTKFLALVCILREESFERKGLIDYNFHCFRKAIHEVFEVGVTSHRSCGHQTSASSLKALTHNGTPRNAI DIP protein Q9HB90 original sequence version: 120 DIP protein Q9HB90 original sequence version: 109 35B3171253CC69CF rraga-rragc-1 2 5 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 8 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association None We employed the two-hybrid method using Rag A as the bait to identify proteins binding to Rag A, and we isolated two novel human G proteins, Rag C and Rag D. rraga-rragd-1 2 7 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association None We employed the two-hybrid method using Rag A as the bait to identify proteins binding to Rag A, and we isolated two novel human G proteins, Rag C and Rag D. rraga-rragc-2 1 8 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q7L523:p.Thr21Leu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain T L Wed May 02 19:21:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr21leu' Tue Jun 30 17:34:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr21Leu' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association 4A and 5A We employed the two-hybrid method using Rag A as the bait to identify proteins binding to Rag A, and we isolated two novel human G proteins, Rag C and Rag D. RAGA mutation glu66leu did not affect the interaction. rraga-rragd-2 1 7 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 unspecified role unspecified role bait bait Q7L523:p.Gln66Leu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 14:31:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln66leu' Tue Jun 30 17:28:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln66Leu' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association 4A We employed the two-hybrid method using Rag A as the bait to identify proteins binding to Rag A, and we isolated two novel human G proteins, Rag C and Rag D. RAGA mutation thr21leu did not affect the interaction. rraga-rragc-3 4 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 rraga-rragd-3 4 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 rragb-rragc-1 4 6 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 rragb-rragd-1 4 6 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3 rragc-rraga-4 4 5 unspecified role unspecified role prey prey region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4B rragd-rraga-4 4 7 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4B rragc-rraga-5 1 8 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 5 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association 5B We employed the two-hybrid method using Rag A as the bait to identify proteins binding to Rag A, and we isolated two novel human G proteins, Rag C and Rag D. RAGA mutation glu66leu did not affect the interaction. rraga-rragc-4 3 5 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role prey prey cytoplasmic region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association 5d GST-Rag A efficiently pulled down the in vitro synthesized C-terminal portion of Rag C (Fig. 5d, lane 6). rragc-rraga-6 3 5 unspecified role unspecified role prey prey cytoplasmic region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 5d GST-Rag C efficiently pulled down the in vitro synthesized C-terminal portion of Rag A protein (Fig. 5d, lane 4).