IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Protein binding and signaling properties of RIN1 suggest a unique effector function. Protein binding and signaling properties of RIN1 suggest a unique effector function. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 imex curation Han L., Wong D., Dhaka A., Afar D., White M., Xie W., Herschman H., Witte O., Colicelli J. Apoptosis - Interactions involving proteins with a function related to apoptosis Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Han L., Wong D., Dhaka A., Afar D., White M., Xie W., Herschman H., Witte O., Colicelli J. Apoptosis - Interactions involving proteins with a function related to apoptosis Accepted 2006-OCT-9 by DAVET. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 Only protein-protein interactions imex curation Protein binding and signaling properties of RIN1 suggest a unique effector function. Protein binding and signaling properties of RIN1 suggest a unique effector function. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 imex curation Han L., Wong D., Dhaka A., Afar D., White M., Xie W., Herschman H., Witte O., Colicelli J. Apoptosis - Interactions involving proteins with a function related to apoptosis Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Han L., Wong D., Dhaka A., Afar D., White M., Xie W., Herschman H., Witte O., Colicelli J. Apoptosis - Interactions involving proteins with a function related to apoptosis Accepted 2006-OCT-9 by DAVET. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 Only protein-protein interactions imex curation Protein binding and signaling properties of RIN1 suggest a unique effector function. Protein binding and signaling properties of RIN1 suggest a unique effector function. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 imex curation Han L., Wong D., Dhaka A., Afar D., White M., Xie W., Herschman H., Witte O., Colicelli J. Apoptosis - Interactions involving proteins with a function related to apoptosis Only protein-protein interactions in vitro In vitro 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Han L., Wong D., Dhaka A., Afar D., White M., Xie W., Herschman H., Witte O., Colicelli J. Apoptosis - Interactions involving proteins with a function related to apoptosis Accepted 2006-OCT-9 by DAVET. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 Only protein-protein interactions imex curation Protein binding and signaling properties of RIN1 suggest a unique effector function. Protein binding and signaling properties of RIN1 suggest a unique effector function. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 imex curation Han L., Wong D., Dhaka A., Afar D., White M., Xie W., Herschman H., Witte O., Colicelli J. Apoptosis - Interactions involving proteins with a function related to apoptosis Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Han L., Wong D., Dhaka A., Afar D., White M., Xie W., Herschman H., Witte O., Colicelli J. Apoptosis - Interactions involving proteins with a function related to apoptosis Accepted 2006-OCT-9 by DAVET. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 Only protein-protein interactions imex curation Protein binding and signaling properties of RIN1 suggest a unique effector function. Protein binding and signaling properties of RIN1 suggest a unique effector function. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 imex curation Han L., Wong D., Dhaka A., Afar D., White M., Xie W., Herschman H., Witte O., Colicelli J. Apoptosis - Interactions involving proteins with a function related to apoptosis Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Han L., Wong D., Dhaka A., Afar D., White M., Xie W., Herschman H., Witte O., Colicelli J. Apoptosis - Interactions involving proteins with a function related to apoptosis Accepted 2006-OCT-9 by DAVET. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 Only protein-protein interactions imex curation Protein binding and signaling properties of RIN1 suggest a unique effector function. Protein binding and signaling properties of RIN1 suggest a unique effector function. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 imex curation Han L., Wong D., Dhaka A., Afar D., White M., Xie W., Herschman H., Witte O., Colicelli J. Apoptosis - Interactions involving proteins with a function related to apoptosis Only protein-protein interactions in vitro In vitro 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Han L., Wong D., Dhaka A., Afar D., White M., Xie W., Herschman H., Witte O., Colicelli J. Apoptosis - Interactions involving proteins with a function related to apoptosis Directed Y2H showed isoform 1 of Rin1 interacts with 14-3-3 epsilon, -beta and -zeta and isoform 2 does not, but the origin of the 14-3-3 proteins could not be traced. Accepted 2006-OCT-9 by DAVET. Mouse cDNA Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 1997 Only protein-protein interactions imex curation abl1_human Tyrosine-protein kinase ABL1 Abelson tyrosine-protein kinase 1 ABL1 ABL JTK7 Abelson murine leukemia viral oncogene homolog 1 Proto-oncogene c-Abl p150 protein protein human Homo sapiens Human MLEICLKLVGCKSKKGLSSSSSCYLEEALQRPVASDFEPQGLSEAARWNSKENLLAGPSENDPNLFVALYDFVASGDNTLSITKGEKLRVLGYNHNGEWCEAQTKNGQGWVPSNYITPVNSLEKHSWYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHHSTVADGLITTLHYPAPKRNKPTVYGVSPNYDKWEMERTDITMKHKLGGGQYGEVYEGVWKKYSLTVAVKTLKEDTMEVEEFLKEAAVMKEIKHPNLVQLLGVCTREPPFYIITEFMTYGNLLDYLRECNRQEVNAVVLLYMATQISSAMEYLEKKNFIHRDLAARNCLVGENHLVKVADFGLSRLMTGDTYTAHAGAKFPIKWTAPESLAYNKFSIKSDVWAFGVLLWEIATYGMSPYPGIDLSQVYELLEKDYRMERPEGCPEKVYELMRACWQWNPSDRPSFAEIHQAFETMFQESSISDEVEKELGKQGVRGAVSTLLQAPELPTKTRTSRRAAEHRDTTDVPEMPHSKGQGESDPLDHEPAVSPLLPRKERGPPEGGLNEDERLLPKDKKTNLFSALIKKKKKTAPTPPKRSSSFREMDGQPERRGAGEEEGRDISNGALAFTPLDTADPAKSPKPSNGAGVPNGALRESGGSGFRSPHLWKKSSTLTSSRLATGEEEGGGSSSKRFLRSCSASCVPHGAKDTEWRSVTLPRDLQSTGRQFDSSTFGGHKSEKPALPRKRAGENRSDQVTRGTVTPPPRLVKKNEEAADEVFKDIMESSPGSSPPNLTPKPLRRQVTVAPASGLPHKEEAGKGSALGTPAAAEPVTPTSKAGSGAPGGTSKGPAEESRVRRHKHSSESPGRDKGKLSRLKPAPPPPPAASAGKAGGKPSQSPSQEAAGEAVLGAKTKATSLVDAVNSDAAKPSQPGEGLKKPVLPATPKPQSAKPSGTPISPAPVPSTLPSASSALAGDQPSSTAFIPLISTRVSLRKTRQPPERIASGAITKGVVLDSTEALCLAISRNSEQMASHSAVLEAGKNLYTFCVSYVDSIQQMRNKFAFREAINKLENNLRELQICPATAGSGPAATQDFSKLLSSVKEISDIVQR DIP protein P00519 original sequence version: 114 DIP protein P00519 original sequence version: 137 DIP protein P00519 original sequence version: 161 DIP protein P00519 original sequence version: 220 DIP protein P00519 original sequence version: 219 85FE6C1C0E483EA2 raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF DIP protein P04049 original sequence version: 117 DIP protein P04049 original sequence version: 106 DIP protein P04049 original sequence version: 114 DIP protein P04049 original sequence version: 141 DIP protein P04049 original sequence version: 142 DIP protein P04049 original sequence version: 147 DIP protein P04049-1 original sequence version: 136 DIP protein P04049 original sequence version: 136 DIP protein P04049 original sequence version: 153 DIP protein P04049 original sequence version: 150 DIP protein P04049 original sequence version: 164 DIP protein P04049 original sequence version: 172 DIP protein P04049-1 original sequence version: 172 DIP protein P04049 original sequence version: 184 DIP protein P04049 original sequence version: 188 DIP protein P04049 original sequence version: 195 EF821B5349711BC3 q13671-1 Ras and Rab interactor 1 RIN1 Ras inhibitor JC99 Ras interaction/interference protein 1 protein protein human Homo sapiens Human MESPGESGAGSPGAPSPSSFTTGHLAREKPAQDPLYDVPNASGGQAGGPQRPGRVVSLRERLLLTRPVWLQLQANAAAALHMLRTEPPGTFLVRKSNTRQCQALCMRLPEASGPSFVSSHYILESPGGVSLEGSELMFPDLVQLICAYCHTRDILLLPLQLPRAIHHAATHKELEAISHLGIEFWSSSLNIKAQRGPAGGPVLPQLKARSPQELDQGTGAALCFFNPLFPGDLGPTKREKFKRSFKVRVSTETSSPLSPPAVPPPPVPVLPGAVPSQTERLPPCQLLRRESSVGYRVPAGSGPSLPPMPSLQEVDCGSPSSSEEEGVPGSRGSPATSPHLGRRRPLLRSMSAAFCSLLAPERQVGRAAAALMQDRHTAAGQLVQDLLTQVRAGPEPQELQGIRQALSRARAMLSAELGPEKLLSPKRLEHVLEKSLHCSVLKPLRPILAARLRRRLAADGSLGRLAEGLRLARAQGPGAFGSHLSLPSPVELEQVRQKLLQLLRTYSPSAQVKRLLQACKLLYMALRTQEGEGAGADEFLPLLSLVLAHCDLPELLLEAEYMSELLEPSLLTGEGGYYLTSLSASLALLSGLGQAHTLPLSPVQELRRSLSLWEQRRLPATHCFQHLLRVAYQDPSSGCTSKTLAVPPEASIATLNQLCATKFRVTQPNTFGLFLYKEQGYHRLPPGALAHRLPTTGYLVYRRAEWPETQGAVTEEEGSGQSEARSRGEEQGCQGDGDAGVKASPRDIREQSETTAEGGQGQAQEGPAQPGEPEAEGSRAAEE DIP protein Q13671-1 original sequence version: 127 2B4DA70147CDDDFE rash_human GTPase HRas HRAS HRAS1 Transforming protein p21 p21ras H-Ras-1 c-H-ras Ha-Ras protein protein human Homo sapiens Human MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREIRQHKLRKLNPPDESGPGCMSCKCVLS DIP protein P01112 original sequence version: 91 DIP protein P01112 original sequence version: 106 DIP protein P01112 original sequence version: 144 DIP protein P01112 original sequence version: 118 DIP protein P01112 original sequence version: 143 DIP protein P01112 original sequence version: 152 DIP protein P01112-1 original sequence version: 159 DIP protein P01112 original sequence version: 170 DIP protein P01112-1 original sequence version: 185 DIP protein P01112 original sequence version: 192 DIP protein P01112-1 original sequence version: 196 DIP protein P01112-1 original sequence version: 202 EE6DC2D933E2856A gnds_mouse Ral guanine nucleotide dissociation stimulator Ralgds Rgds Ral guanine nucleotide exchange factor protein protein mouse Mus musculus Mouse MMVDCQSSTQEIGEELINGVIYSISLRKVQLHQGATKGQRWLGCENESALNLYETCKVRTVKAGTLEKLVEHLVPAFQGSDLSYVTVFLCTYRAFTTTQQVLDLLFKRYGRCDALTASSRYGCILPYSSEDGGPQDQLKNAISSILGTWLDQYSEDFCQPPDFPCLKQLVAYVQLNMPGSDLERRAHLLLAQLEDLEPSEAESEALSPAPVLSLKPASQLEPALLLTPSQVVTSTPVREPAAAPVPVLASSPVVAPAPELEPVPEPPQEPEPSLALAPELEPAVSQSLELESAPVPTPALEPSWSLPEATENGLTEKPHLLLFPPDLVAEQFTLMDAELFKKVVPYHCLGSIWSQRDKKGKEHLAPTIRATVAQFNNVANCVITTCLGDQSMKAPDRARVVEHWIEVARECRALKNFSSLYAILSALQSNAIHRLKKTWEEVSRDSFRVFQKLSEIFSDENNYSLSRELLIKEGTSKFATLEMNPRRAQRRQKETGVIQGTVPYLGTFLTDLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKLLQSACNNYSIAPEEHFGTWFRAMERLSEAESYTLSCELEPPSESASNTLRSKKSTAIVKRWSDRQAPSTELSTSSSAHSKSCDQLRCSPYLGSGDITDALSVHSAGSSSSDVEEINMSFVPESPDGQEKKFWESASQSSPETSGISSASSSTSSSSASTTPVSTTRTHKRSVSGVCSYSSSLPLYNQQVGDCCIIRVSLDVDNGNMYKSILVTSQDKAPTVIRKAMDKHNLDEDEPEDYELVQIISEDHKLKIPENANVFYAMNSTANYDFILKKRTFTKGAKVKHGASSTLPRMKQKGLRIAKGIF 5C6731F7BF3B07B8 1433e_mouse 14-3-3 protein epsilon Ywhae protein protein mouse Mus musculus Mouse MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ 07817CCBD1F75B26 rin1_human Ras and Rab interactor 1 RIN1 Ras interaction/interference protein 1 Ras inhibitor JC99 protein protein human Homo sapiens Human MESPGESGAGSPGAPSPSSFTTGHLAREKPAQDPLYDVPNASGGQAGGPQRPGRVVSLRERLLLTRPVWLQLQANAAAALHMLRTEPPGTFLVRKSNTRQCQALCMRLPEASGPSFVSSHYILESPGGVSLEGSELMFPDLVQLICAYCHTRDILLLPLQLPRAIHHAATHKELEAISHLGIEFWSSSLNIKAQRGPAGGPVLPQLKARSPQELDQGTGAALCFFNPLFPGDLGPTKREKFKRSFKVRVSTETSSPLSPPAVPPPPVPVLPGAVPSQTERLPPCQLLRRESSVGYRVPAGSGPSLPPMPSLQEVDCGSPSSSEEEGVPGSRGSPATSPHLGRRRPLLRSMSAAFCSLLAPERQVGRAAAALMQDRHTAAGQLVQDLLTQVRAGPEPQELQGIRQALSRARAMLSAELGPEKLLSPKRLEHVLEKSLHCSVLKPLRPILAARLRRRLAADGSLGRLAEGLRLARAQGPGAFGSHLSLPSPVELEQVRQKLLQLLRTYSPSAQVKRLLQACKLLYMALRTQEGEGAGADEFLPLLSLVLAHCDLPELLLEAEYMSELLEPSLLTGEGGYYLTSLSASLALLSGLGQAHTLPLSPVQELRRSLSLWEQRRLPATHCFQHLLRVAYQDPSSGCTSKTLAVPPEASIATLNQLCATKFRVTQPNTFGLFLYKEQGYHRLPPGALAHRLPTTGYLVYRRAEWPETQGAVTEEEGSGQSEARSRGEEQGCQGDGDAGVKASPRDIREQSETTAEGGQGQAQEGPAQPGEPEAEGSRAAEE 2B4DA70147CDDDFE rin1-hras-1 5 13 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait P01112:p.Gln61Leu mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 15:35:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln61leu' Tue Jun 30 19:07:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln61Leu' physical association physical association P01112 transfection; Q13671 transfection; 2A When RAS is immunoprecipitated from these cells (using antibody Y238), RIN1 was also present (Fig. 2A). A constitutively active form of H-Ras (Gly12Val) was sued in this experiment. rin1-hras-2 5 10 unspecified role unspecified role bait bait P01112:p.Gln61Leu mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 17:04:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln61leu' Tue Jun 30 19:23:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln61Leu' 13 unspecified role unspecified role prey prey ras binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association P01112 transfection; Q13671 transfection; 2B The interaction of RAS with RIN1 was confirmed by copurification of activated RAS with an epitope tagged form of the carboxyl terminus of RIN1 (RIN1C) from cells expressing these proteins (Fig. 2B). rin1-hras-3 1 10 unspecified role unspecified role prey prey P01112:p.Gln61Leu mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 19:35:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln61leu' Tue Jun 30 19:03:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln61Leu' 13 unspecified role unspecified role bait bait ras binding region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis range range certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association P01112 transfection; Q13671 transfection; 2B The interaction of RAS with RIN1 was confirmed by copurification of activated RAS with an epitope tagged form of the carboxyl terminus of RIN1 (RIN1C) from cells expressing these proteins (Fig. 2B). rin1-hras-4 3 10 unspecified role unspecified role bait bait P01112:p.Thr35Ser mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T S Wed May 02 14:49:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr35ser' Tue Jun 30 18:27:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr35Ser' P01112:p.Tyr40Cys mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y C Wed May 02 16:23:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr40cys' Tue Jun 30 17:27:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr40Cys' 13 unspecified role unspecified role prey prey physical association physical association Table 1 We tested the ability of RIN1 to interact with H-RAS effector domain mutants using the two-hybrid detection system (Table 1). raf1-hras-4 3 8 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait P01112:p.Glu37Gly mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E G Wed May 02 17:53:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu37gly' Tue Jun 30 16:25:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu37Gly' P01112:p.Tyr40Cys mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y C Wed May 02 19:01:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr40cys' Tue Jun 30 19:15:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr40Cys' physical association physical association Table 1 RIN1, like RAF1, interacts well with both wild-type and the constitutively active mutant form of RAS (H-RASV12). ralgds-hras-1 3 11 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait P01112:p.Glu37Gly mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E G Wed May 02 20:21:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu37gly' Tue Jun 30 15:49:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu37Gly' P01112:p.Tyr40Cys mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y C Wed May 02 16:44:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr40cys' Tue Jun 30 16:29:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr40Cys' physical association physical association Table 1 The putative RAS effector RalGDS had an interaction profile identical to RIN1 (Table 1; ref. 28). rin1-hras-5 4 9 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role prey prey physical association physical association Fig. 3 The minimum RAS binding domain of RIN1, therefore, appears to lie within residues 294�727, and optimal binding requires some or all of the 62 aa that are missing in the internal splicing variant. ywhae-rin1-1 6 12 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait ras binding domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association We asked whether the RBD of RIN1 is also involved in other protein-protein interactions. Using the two hybrid procedure (41), we isolated eight distinct clones from a murine cDNA library. One of the most frequently isolated cDNAs encodes the epsilon isoform of 14-3-3. 4 abl1-rin1-4 2 7 enzyme enzyme unspecified role unspecified role spofr-sf_21 Spodoptera frugiperda insect cells sf_21 Insect cells derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae. full name: IPLB-SF-21-AE cells are susceptible to Baculovirus infection. 13 enzyme target enzyme target substrate substrate unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region (possible) optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy mutation analysis mutation analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined phosphorylation phosphorylation reaction phosphorylation Further phosphorylation of Rin1 (Q13671) 7 RIN1N is a substrate and binding partner for c-ABL