IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The RIP1/RIP3 Necrosome Forms a Functional Amyloid Signaling Complex Required for Programmed Necrosis. The RIP1/RIP3 Necrosome Forms a Functional Amyloid Signaling Complex Required for Programmed Necrosis. Cell (0092-8674) 2012 imex curation Li J., McQuade T., Siemer A.B., Napetschnig J., Moriwaki K., Hsiao Y.S., Damko E., Moquin D., Walz T., McDermott A., Chan F.K., Wu H. Apoptosis - Interactions involving proteins with a function related to apoptosis In vitro kinase assays not captured as source of myelin basic protein could not be traced. hao.wu@childrens.harvard.edu 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions ecoli Escherichia coli (strain K12) comigration in sds comigration in sds page comigration in sds weight by comassie molecular weight estimation by coomasie staining weight by comassie Li J., McQuade T., Siemer A.B., Napetschnig J., Moriwaki K., Hsiao Y.S., Damko E., Moquin D., Walz T., McDermott A., Chan F.K., Wu H. Apoptosis - Interactions involving proteins with a function related to apoptosis In vitro kinase assays not captured as source of myelin basic protein could not be traced. hao.wu@childrens.harvard.edu Accepted 2012-AUG-16 by PPORRAS 19-Sept-2012: Contacted by IntAct-Help. Cell (0092-8674) 2012 Only protein-protein interactions imex curation The RIP1/RIP3 Necrosome Forms a Functional Amyloid Signaling Complex Required for Programmed Necrosis. The RIP1/RIP3 Necrosome Forms a Functional Amyloid Signaling Complex Required for Programmed Necrosis. Cell (0092-8674) 2012 imex curation Li J., McQuade T., Siemer A.B., Napetschnig J., Moriwaki K., Hsiao Y.S., Damko E., Moquin D., Walz T., McDermott A., Chan F.K., Wu H. Apoptosis - Interactions involving proteins with a function related to apoptosis In vitro kinase assays not captured as source of myelin basic protein could not be traced. hao.wu@childrens.harvard.edu 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro cd circular dichroism CD CD cd predetermined predetermined participant predetermined Li J., McQuade T., Siemer A.B., Napetschnig J., Moriwaki K., Hsiao Y.S., Damko E., Moquin D., Walz T., McDermott A., Chan F.K., Wu H. Apoptosis - Interactions involving proteins with a function related to apoptosis In vitro kinase assays not captured as source of myelin basic protein could not be traced. It has been assumed that the proteins with tags removed (as described for the gel filtration experiment) have been used for subsequent analyses throughout when the homodimers are under investigation. hao.wu@childrens.harvard.edu Accepted 2012-AUG-16 by PPORRAS 19-Sept-2012: Contacted by IntAct-Help. Cell (0092-8674) 2012 Only protein-protein interactions imex curation The RIP1/RIP3 Necrosome Forms a Functional Amyloid Signaling Complex Required for Programmed Necrosis. The RIP1/RIP3 Necrosome Forms a Functional Amyloid Signaling Complex Required for Programmed Necrosis. Cell (0092-8674) 2012 imex curation Li J., McQuade T., Siemer A.B., Napetschnig J., Moriwaki K., Hsiao Y.S., Damko E., Moquin D., Walz T., McDermott A., Chan F.K., Wu H. Apoptosis - Interactions involving proteins with a function related to apoptosis In vitro kinase assays not captured as source of myelin basic protein could not be traced. hao.wu@childrens.harvard.edu 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions ecoli Escherichia coli (strain K12) molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Li J., McQuade T., Siemer A.B., Napetschnig J., Moriwaki K., Hsiao Y.S., Damko E., Moquin D., Walz T., McDermott A., Chan F.K., Wu H. Apoptosis - Interactions involving proteins with a function related to apoptosis In vitro kinase assays not captured as source of myelin basic protein could not be traced. Proteins first purified by His-tag pulldown. hao.wu@childrens.harvard.edu Accepted 2012-AUG-16 by PPORRAS 19-Sept-2012: Contacted by IntAct-Help. Cell (0092-8674) 2012 Only protein-protein interactions imex curation The RIP1/RIP3 Necrosome Forms a Functional Amyloid Signaling Complex Required for Programmed Necrosis. The RIP1/RIP3 Necrosome Forms a Functional Amyloid Signaling Complex Required for Programmed Necrosis. Cell (0092-8674) 2012 imex curation Li J., McQuade T., Siemer A.B., Napetschnig J., Moriwaki K., Hsiao Y.S., Damko E., Moquin D., Walz T., McDermott A., Chan F.K., Wu H. Apoptosis - Interactions involving proteins with a function related to apoptosis In vitro kinase assays not captured as source of myelin basic protein could not be traced. hao.wu@childrens.harvard.edu 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro fluorescence fluorescence technology fluorescence predetermined predetermined participant predetermined Li J., McQuade T., Siemer A.B., Napetschnig J., Moriwaki K., Hsiao Y.S., Damko E., Moquin D., Walz T., McDermott A., Chan F.K., Wu H. Apoptosis - Interactions involving proteins with a function related to apoptosis In vitro kinase assays not captured as source of myelin basic protein could not be traced. It has been assumed that the proteins with tags removed (as described for the gel filtration experiment) have been used for subsequent analyses throughout when the homodimers are under investigation. hao.wu@childrens.harvard.edu Accepted 2012-AUG-16 by PPORRAS 19-Sept-2012: Contacted by IntAct-Help. Cell (0092-8674) 2012 Only protein-protein interactions imex curation The RIP1/RIP3 Necrosome Forms a Functional Amyloid Signaling Complex Required for Programmed Necrosis. The RIP1/RIP3 Necrosome Forms a Functional Amyloid Signaling Complex Required for Programmed Necrosis. Cell (0092-8674) 2012 imex curation Li J., McQuade T., Siemer A.B., Napetschnig J., Moriwaki K., Hsiao Y.S., Damko E., Moquin D., Walz T., McDermott A., Chan F.K., Wu H. Apoptosis - Interactions involving proteins with a function related to apoptosis In vitro kinase assays not captured as source of myelin basic protein could not be traced. hao.wu@childrens.harvard.edu 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Li J., McQuade T., Siemer A.B., Napetschnig J., Moriwaki K., Hsiao Y.S., Damko E., Moquin D., Walz T., McDermott A., Chan F.K., Wu H. Apoptosis - Interactions involving proteins with a function related to apoptosis In vitro kinase assays not captured as source of myelin basic protein could not be traced. hao.wu@childrens.harvard.edu Accepted 2012-AUG-16 by PPORRAS 19-Sept-2012: Contacted by IntAct-Help. Cell (0092-8674) 2012 Only protein-protein interactions imex curation The RIP1/RIP3 Necrosome Forms a Functional Amyloid Signaling Complex Required for Programmed Necrosis. The RIP1/RIP3 Necrosome Forms a Functional Amyloid Signaling Complex Required for Programmed Necrosis. Cell (0092-8674) 2012 imex curation Li J., McQuade T., Siemer A.B., Napetschnig J., Moriwaki K., Hsiao Y.S., Damko E., Moquin D., Walz T., McDermott A., Chan F.K., Wu H. Apoptosis - Interactions involving proteins with a function related to apoptosis In vitro kinase assays not captured as source of myelin basic protein could not be traced. hao.wu@childrens.harvard.edu 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions human-ht_29 Homo sapiens HT-29 caucasian women with colon adenocarcinoma ht_29 HT-29 confirmed as human with IEF of AST, LDH established from the primary tumor of a 44-year-old Caucasian woman with colon adenocarcinoma in 1964; described to be heterotransplantable forming well-differentiated grade I tumors anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li J., McQuade T., Siemer A.B., Napetschnig J., Moriwaki K., Hsiao Y.S., Damko E., Moquin D., Walz T., McDermott A., Chan F.K., Wu H. Apoptosis - Interactions involving proteins with a function related to apoptosis In vitro kinase assays not captured as source of myelin basic protein could not be traced. hao.wu@childrens.harvard.edu Accepted 2012-AUG-16 by PPORRAS 19-Sept-2012: Contacted by IntAct-Help. Cell (0092-8674) 2012 Only protein-protein interactions imex curation The RIP1/RIP3 Necrosome Forms a Functional Amyloid Signaling Complex Required for Programmed Necrosis. The RIP1/RIP3 Necrosome Forms a Functional Amyloid Signaling Complex Required for Programmed Necrosis. Cell (0092-8674) 2012 imex curation Li J., McQuade T., Siemer A.B., Napetschnig J., Moriwaki K., Hsiao Y.S., Damko E., Moquin D., Walz T., McDermott A., Chan F.K., Wu H. Apoptosis - Interactions involving proteins with a function related to apoptosis In vitro kinase assays not captured as source of myelin basic protein could not be traced. hao.wu@childrens.harvard.edu 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro solid state nmr solid state nmr ssnmr solid state nmr predetermined predetermined participant predetermined Li J., McQuade T., Siemer A.B., Napetschnig J., Moriwaki K., Hsiao Y.S., Damko E., Moquin D., Walz T., McDermott A., Chan F.K., Wu H. Apoptosis - Interactions involving proteins with a function related to apoptosis In vitro kinase assays not captured as source of myelin basic protein could not be traced. hao.wu@childrens.harvard.edu Accepted 2012-AUG-16 by PPORRAS 19-Sept-2012: Contacted by IntAct-Help. Cell (0092-8674) 2012 Only protein-protein interactions imex curation The RIP1/RIP3 Necrosome Forms a Functional Amyloid Signaling Complex Required for Programmed Necrosis. The RIP1/RIP3 Necrosome Forms a Functional Amyloid Signaling Complex Required for Programmed Necrosis. Cell (0092-8674) 2012 imex curation Li J., McQuade T., Siemer A.B., Napetschnig J., Moriwaki K., Hsiao Y.S., Damko E., Moquin D., Walz T., McDermott A., Chan F.K., Wu H. Apoptosis - Interactions involving proteins with a function related to apoptosis In vitro kinase assays not captured as source of myelin basic protein could not be traced. hao.wu@childrens.harvard.edu 19-Sept-2012: Contacted by IntAct-Help. Only protein-protein interactions trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Li J., McQuade T., Siemer A.B., Napetschnig J., Moriwaki K., Hsiao Y.S., Damko E., Moquin D., Walz T., McDermott A., Chan F.K., Wu H. Apoptosis - Interactions involving proteins with a function related to apoptosis In vitro kinase assays not captured as source of myelin basic protein could not be traced. hao.wu@childrens.harvard.edu Accepted 2012-AUG-16 by PPORRAS 19-Sept-2012: Contacted by IntAct-Help. Cell (0092-8674) 2012 Only protein-protein interactions imex curation ripk3_human Receptor-interacting serine/threonine-protein kinase 3 RIPK3 RIP3 RIP-like protein kinase 3 Receptor-interacting protein 3 protein protein human Homo sapiens Human MSCVKLWPSGAPAPLVSIEELENQELVGKGGFGTVFRAQHRKWGYDVAVKIVNSKAISREVKAMASLDNEFVLRLEGVIEKVNWDQDPKPALVTKFMENGSLSGLLQSQCPRPWPLLCRLLKEVVLGMFYLHDQNPVLLHRDLKPSNVLLDPELHVKLADFGLSTFQGGSQSGTGSGEPGGTLGYLAPELFVNVNRKASTASDVYSFGILMWAVLAGREVELPTEPSLVYEAVCNRQNRPSLAELPQAGPETPGLEGLKELMQLCWSSEPKDRPSFQECLPKTDEVFQMVENNMNAAVSTVKDFLSQLRSSNRRFSIPESGQGGTEMDGFRRTIENQHSRNDVMVSEWLNKLNLEEPPSSVPKKCPSLTKRSRAQEEQVPQAWTAGTSSDSMAQPPQTPETSTFRNQMPSPTSTGTPSPGPRGNQGAERQGMNWSCRTPEPNPVTGRPLVNIYNCSGVQVGDNNYLTMQQTTALPTWGLAPSGKGRGLQHPPPVGSQEGPKDPEAWSRPQGWYNHSGK DIP protein Q9Y572 original sequence version: 134 BF755F8A0B1810A1 ripk1_human Receptor-interacting serine/threonine-protein kinase 1 RIPK1 RIP Cell death protein RIP RIP1 Receptor-interacting protein 1 protein protein human Homo sapiens Human MQPDMSLNVIKMKSSDFLESAELDSGGFGKVSLCFHRTQGLMIMKTVYKGPNCIEHNEALLEEAKMMNRLRHSRVVKLLGVIIEEGKYSLVMEYMEKGNLMHVLKAEMSTPLSVKGRIILEIIEGMCYLHGKGVIHKDLKPENILVDNDFHIKIADLGLASFKMWSKLNNEEHNELREVDGTAKKNGGTLYYMAPEHLNDVNAKPTEKSDVYSFAVVLWAIFANKEPYENAICEQQLIMCIKSGNRPDVDDITEYCPREIISLMKLCWEANPEARPTFPGIEEKFRPFYLSQLEESVEEDVKSLKKEYSNENAVVKRMQSLQLDCVAVPSSRSNSATEQPGSLHSSQGLGMGPVEESWFAPSLEHPQEENEPSLQSKLQDEANYHLYGSRMDRQTKQQPRQNVAYNREEERRRRVSHDPFAQQRPYENFQNTEGKGTAYSSAASHGNAVHQPSGLTSQPQVLYQNNGLYSSHGFGTRPLDPGTAGPRVWYRPIPSHMPSLHNIPVPETNYLGNTPTMPFSSLPPTDESIKYTIYNSTGIQIGAYNYMEIGGTSSSLLDSTNTNFKEEPAAKYQAIFDNTTSLTDKHLDPIRENLGKHWKNCARKLGFTQSQIDEIDHDYERDGLKEKVYQMLQKWVMREGIKGATVGKLAQALHQCSRIDLLSSLIYVSQN DIP protein Q13546 original sequence version: 158 DIP protein Q13546-1 original sequence version: 140 DIP protein Q13546 original sequence version: 146 DIP protein Q13546-1 original sequence version: 146 DIP protein Q13546 original sequence version: 134 DIP protein Q13546 original sequence version: 109 976E2428D525A9B2 zbp1_human Z-DNA-binding protein 1 ZBP1 C20orf183 DLM1 Tumor stroma and activated macrophage protein DLM-1 DNA-dependent activator of IFN-regulatory factors protein protein human Homo sapiens Human MAQAPADPGREGHLEQRILQVLTEAGSPVKLAQLVKECQAPKRELNQVLYRMKKELKVSLTSPATWCLGGTDPEGEGPAELALSSPAERPQQHAATIPETPGPQFSQQREEDIYRFLKDNGPQRALVIAQALGMRTAKDVNRDLYRMKSRHLLDMDEQSKAWTIYRPEDSGRRAKSASIIYQHNPINMICQNGPNSWISIANSEAIQIGHGNIITRQTVSREDGSAGPRHLPSMAPGDSSTWGTLVDPWGPQDIHMEQSILRRVQLGHSNEMRLHGVPSEGPAHIPPGSPPVSATAAGPEASFEARIPSPGTHPEGEAAQRIHMKSCFLEDATIGNSNKMSISPGVAGPGGVAGSGEGEPGEDAGRRPADTQSRSHFPRDIGQPITPSHSKLTPKLETMTLGNRSHKAAEGSHYVDEASHEGSWWGGGI 2C9B195112822991 tnr1a_human Tumor necrosis factor receptor superfamily member 1A TNFRSF1A TNFAR TNFR1 p60 Tumor necrosis factor receptor 1 Tumor necrosis factor receptor type I p55 protein protein human Homo sapiens Human MGLSTVPDLLLPLVLLELLVGIYPSGVIGLVPHLGDREKRDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIENVKGTEDSGTTVLLPLVIFFGLCLLSLLFIGLMYRYQRWKSKLYSIVCGKSTPEKEGELEGTTTKPLAPNPSFSPTPGFTPTLGFSPVPSSTFTSSSTYTPGDCPNFAAPRREVAPPYQGADPILATALASDPIPNPLQKWEDSAHKPQSLDTDDPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR DIP protein P19438 original sequence version: 135 DIP protein P19438 original sequence version: 150 DIP protein P19438 original sequence version: 177 4CEFBA96D03B8225 ripk1-ripk3-11 3 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9Y572:p.Ser456Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S D Wed May 02 20:17:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser456asp' Tue Jun 30 16:44:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser456Asp' Q9Y572:p.Val458Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V D Wed May 02 19:02:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val458asp' Tue Jun 30 18:01:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val458Asp' Q9Y572:p.Val460Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V D Wed May 02 15:27:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val460asp' Tue Jun 30 19:28:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val460Asp' Q9Y572:p.Gly461Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G D Wed May 02 19:40:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly461asp' Tue Jun 30 19:04:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly461Asp' Q9Y572:p.Asp462Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D K Wed May 02 14:37:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp462lys' Tue Jun 30 19:43:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp462Lys' Q9Y572:p.[Val458Glu;Val460Glu] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V E certain certain sequence position certain certain certain sequence position certain V E Wed May 02 16:53:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val458glu,val460glu' Tue Jun 30 19:29:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val458Glu;Val460Glu]' Q9Y572:p.Gly457Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G D Wed May 02 15:49:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly457asp' Tue Jun 30 18:33:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly457Asp' Q9Y572:p.Gln459Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q D Wed May 02 19:00:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln459asp' Tue Jun 30 18:03:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln459Asp' ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sumo tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 25 15 physical association physical association Fig. 1C, 4A, 5B, Supp. Fig. 5C ripk1-ripk3-12 5 10 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain sumo tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 34 30 direct interaction direct interaction Fig. 1D, 1E ripk1-ripk3-13 4 10 unspecified role unspecified role neutral component neutral component sumo tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2A-C, 4C, Supp. Figs. 2, 4B, 4C Classified as an amyloid by binding of aromatic, cross-β binding dyes Thioflavin T (ThT) and Congo red ripk1-ripk3-14 2 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain sumo tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain sumo tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction Fig. 2D Structure is a mixture of β sheet and random coil structures. ripk3-ripk1-3 8 10 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 53 55 direct interaction direct interaction Fig. 3A, Supp. Fig. 3A ripk1-ripk3-15 8 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 60 63 direct interaction direct interaction Fig. 3A, Supp. Fig. 3B ripk1-ripk1 3 10 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 67 69 physical association physical association Fig. 4A ripk3-ripk3 3 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 74 72 physical association physical association Fig. 4A ripk3-ripk3-1 5 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sumo tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sumo tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 4B ripk1-ripk1-1 5 10 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q13546:p.Ile539Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I D Wed May 02 15:57:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile539asp' Tue Jun 30 17:59:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile539Asp' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 10 unspecified role unspecified role neutral component neutral component Q13546:p.Ile539Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I D Wed May 02 20:17:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile539asp' Tue Jun 30 18:14:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile539Asp' n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig. 5D Upon subtilisin digestion, EM of WT RIP1-FL showed filamentous structures, though they were apparently less regular than the RIP1/RIP3 complex. In contrast, the RIP1-FL mutant I539D was mostly degraded by subtilisin and showed only residual aggregates ripk1-ripk1-3 4 10 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 93 95 direct interaction direct interaction Fig. 4C Supp. Figs. 2, 4B, 4C Classified as an amyloid by binding of aromatic, cross-β binding dyes Thioflavin T (ThT) and Congo red ripk3-ripk3-3 4 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 100 98 direct interaction direct interaction Fig. 2A-C, 4C, Supp. Figs. 2, 4B, 4C Classified as an amyloid by binding of aromatic, cross-β binding dyes Thioflavin T (ThT) and Congo red ripk3-ripk3-4 2 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 105 103 direct interaction direct interaction Supp. Fig. 4A ripk1-ripk1-4 2 10 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 108 110 direct interaction direct interaction Supp. Fig. 4A Classified as an amyloid by binding of aromatic, cross-β binding dyes Thioflavin T (ThT) and Congo red ripk1-ripk3-16 7 10 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain sumo tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction Fig. 3B, Supp. Figs. 3C, 3D ripk1-ripk3-17 3 10 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q13546:p.Ile539Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I D Wed May 02 18:09:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile539asp' Tue Jun 30 15:49:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile539Asp' Q13546:p.Gly542Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G D Wed May 02 14:31:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly542asp' Tue Jun 30 16:51:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly542Asp' Q13546:p.Ala543Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain A D Wed May 02 14:31:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala543asp' Tue Jun 30 18:13:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala543Asp' sumo tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q13546:p.Gln540Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q D Wed May 02 18:58:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln540asp' Tue Jun 30 20:02:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln540Asp' Q13546:p.Tyr544Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y D Wed May 02 17:49:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr544asp' Tue Jun 30 20:05:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr544Asp' Q13546:p.Asn545Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain N D Wed May 02 20:04:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn545asp' Tue Jun 30 18:18:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn545Asp' Q13546:p.Ile541Asp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I D Wed May 02 18:21:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile541asp' Tue Jun 30 18:38:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile541Asp' ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 125 131 physical association physical association Fig. 5A, Supp. Fig. 5A, 5B ripk1-ripk3-18 1 10 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sumo tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component Q9Y572:p.Gly461Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G P Wed May 02 14:35:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly461pro' Tue Jun 30 19:58:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly461Pro' Q9Y572:p.Val458Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V P Wed May 02 19:22:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val458pro' Tue Jun 30 19:07:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val458Pro' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9Y572:p.Gln459Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 16:11:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln459pro' Tue Jun 30 17:30:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln459Pro' Q9Y572:p.Val460Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V P Wed May 02 19:00:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val460pro' Tue Jun 30 17:56:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val460Pro' ecoli Escherichia coli (strain K12) physical association physical association Supp. Fig. 5D ripk1-ripk1-5 5 10 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range sumo tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain sumo tag tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 150 145 direct interaction direct interaction Fig. 4B Upon subtilisin digestion, EM of WT RIP1-FL showed filamentous structures, though they were apparently less regular than the RIP1/RIP3 complex. In contrast, the RIP1-FL mutant I539D was mostly degraded by subtilisin and showed only residual aggregates ripk3-ripk3-5 5 9 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9Y572:p.Val460Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V P Wed May 02 15:10:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val460pro' Tue Jun 30 15:39:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val460Pro' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 9 unspecified role unspecified role neutral component neutral component Q9Y572:p.Val460Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V P Wed May 02 16:01:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val460pro' Tue Jun 30 18:13:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val460Pro' n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig. 5D Upon subtilisin digestion, EM of WT RIP3-FL showed filamentous structures, though they were apparently less regular than the RIP1/RIP3 complex. In contrast, the RIP3-FL mutant V460P was mostly degraded by subtilisin and showed only residual aggregates ripk1-ripk3-19 6 10 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association TNF, zVAD-fmk (caspase inhibitor), and LBW242 (Smac mimetic) Fig. 6A, Supp. Fig. 6D RIPK3 becomes hyper-phosphorylated with time. ripk3-ripk1-4 6 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association TNF, zVAD-fmk (caspase inhibitor), and LBW242 (Smac mimetic) Fig. 6B 4 M urea or 8 M urea did not disrupt the interaction between RIP3 and RIP1, whereas 150 mM NaOH did. tnfrsf1a-ripk1-4 6 10 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association TNF Fig. 6C The interaction between RIP1 and TNFR1 was completely abolished in all conditions, including 4 M urea. ripk1-zbp1 5 11 unspecified role unspecified role neutral component neutral component region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Fig. 7E ripk1-ripk3-20 3 10 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 9 unspecified role unspecified role neutral component neutral component n-terminal mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini Trichoplusia ni (Cabbage looper) Cabbage looper physical association physical association Fig. 1C