UniProt UniProt http://www.uniprot.org SIRT1 Deacetylates the DNA Methyltransferase 1 (DNMT1) Protein and Alters its Activities. SIRT1 Deacetylates the DNA Methyltransferase 1 (DNMT1) Protein and Alters its Activities. Mol. Cell. Biol. (0270-7306) 2011 mimix curation Peng L., Yuan Z., Ling H., Fukasawa K., Robertson K., Olashaw N., Koomen J., Chen J., Lane W.S., Seto E. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of GFP-SIRT1 constructs as human according sequence length (fig 3). Origin of GST-SIRT1 (fig 2) as human according pmid 12006491 Origin of Flag-PCAF (fig 1) as human according 8684459 (as in UniProt annotation / article not free available online) ed.seto@moffitt.org 21-Dec-2011: Contacted by JYOTI. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 imaging technique imaging technique microscopy immunostaining immunostaining Peng L., Yuan Z., Ling H., Fukasawa K., Robertson K., Olashaw N., Koomen J., Chen J., Lane W.S., Seto E. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of GFP-SIRT1 constructs as human according sequence length (fig 3). Origin of GST-SIRT1 (fig 2) as human according pmid 12006491 Origin of Flag-PCAF (fig 1) as human according 8684459 (as in UniProt annotation / article not free available online) ed.seto@moffitt.org Accepted 2011-OCT-18 by JYOTI 21-Dec-2011: Contacted by JYOTI. Mol. Cell. Biol. (0270-7306) 2011 mimix curation SIRT1 Deacetylates the DNA Methyltransferase 1 (DNMT1) Protein and Alters its Activities. SIRT1 Deacetylates the DNA Methyltransferase 1 (DNMT1) Protein and Alters its Activities. Mol. Cell. Biol. (0270-7306) 2011 mimix curation Peng L., Yuan Z., Ling H., Fukasawa K., Robertson K., Olashaw N., Koomen J., Chen J., Lane W.S., Seto E. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of GFP-SIRT1 constructs as human according sequence length (fig 3). Origin of GST-SIRT1 (fig 2) as human according pmid 12006491 Origin of Flag-PCAF (fig 1) as human according 8684459 (as in UniProt annotation / article not free available online) ed.seto@moffitt.org 21-Dec-2011: Contacted by JYOTI. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Peng L., Yuan Z., Ling H., Fukasawa K., Robertson K., Olashaw N., Koomen J., Chen J., Lane W.S., Seto E. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of GFP-SIRT1 constructs as human according sequence length (fig 3). Origin of GST-SIRT1 (fig 2) as human according pmid 12006491 Origin of Flag-PCAF (fig 1) as human according 8684459 (as in UniProt annotation / article not free available online) ed.seto@moffitt.org Accepted 2011-OCT-18 by JYOTI 21-Dec-2011: Contacted by JYOTI. Mol. Cell. Biol. (0270-7306) 2011 mimix curation SIRT1 Deacetylates the DNA Methyltransferase 1 (DNMT1) Protein and Alters its Activities. SIRT1 Deacetylates the DNA Methyltransferase 1 (DNMT1) Protein and Alters its Activities. Mol. Cell. Biol. (0270-7306) 2011 mimix curation Peng L., Yuan Z., Ling H., Fukasawa K., Robertson K., Olashaw N., Koomen J., Chen J., Lane W.S., Seto E. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of GFP-SIRT1 constructs as human according sequence length (fig 3). Origin of GST-SIRT1 (fig 2) as human according pmid 12006491 Origin of Flag-PCAF (fig 1) as human according 8684459 (as in UniProt annotation / article not free available online) ed.seto@moffitt.org 21-Dec-2011: Contacted by JYOTI. in vitro In vitro deacetylase assay deacetylase assay predetermined predetermined participant predetermined Peng L., Yuan Z., Ling H., Fukasawa K., Robertson K., Olashaw N., Koomen J., Chen J., Lane W.S., Seto E. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of GFP-SIRT1 constructs as human according sequence length (fig 3). Origin of GST-SIRT1 (fig 2) as human according pmid 12006491 Origin of Flag-PCAF (fig 1) as human according 8684459 (as in UniProt annotation / article not free available online) ed.seto@moffitt.org Accepted 2011-OCT-18 by JYOTI 21-Dec-2011: Contacted by JYOTI. Mol. Cell. Biol. (0270-7306) 2011 mimix curation SIRT1 Deacetylates the DNA Methyltransferase 1 (DNMT1) Protein and Alters its Activities. SIRT1 Deacetylates the DNA Methyltransferase 1 (DNMT1) Protein and Alters its Activities. Mol. Cell. Biol. (0270-7306) 2011 mimix curation Peng L., Yuan Z., Ling H., Fukasawa K., Robertson K., Olashaw N., Koomen J., Chen J., Lane W.S., Seto E. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of GFP-SIRT1 constructs as human according sequence length (fig 3). Origin of GST-SIRT1 (fig 2) as human according pmid 12006491 Origin of Flag-PCAF (fig 1) as human according 8684459 (as in UniProt annotation / article not free available online) ed.seto@moffitt.org 21-Dec-2011: Contacted by JYOTI. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Peng L., Yuan Z., Ling H., Fukasawa K., Robertson K., Olashaw N., Koomen J., Chen J., Lane W.S., Seto E. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of GFP-SIRT1 constructs as human according sequence length (fig 3). Origin of GST-SIRT1 (fig 2) as human according pmid 12006491 Origin of Flag-PCAF (fig 1) as human according 8684459 (as in UniProt annotation / article not free available online) ed.seto@moffitt.org Accepted 2011-OCT-18 by JYOTI 21-Dec-2011: Contacted by JYOTI. Mol. Cell. Biol. (0270-7306) 2011 mimix curation SIRT1 Deacetylates the DNA Methyltransferase 1 (DNMT1) Protein and Alters its Activities. SIRT1 Deacetylates the DNA Methyltransferase 1 (DNMT1) Protein and Alters its Activities. Mol. Cell. Biol. (0270-7306) 2011 mimix curation Peng L., Yuan Z., Ling H., Fukasawa K., Robertson K., Olashaw N., Koomen J., Chen J., Lane W.S., Seto E. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of GFP-SIRT1 constructs as human according sequence length (fig 3). Origin of GST-SIRT1 (fig 2) as human according pmid 12006491 Origin of Flag-PCAF (fig 1) as human according 8684459 (as in UniProt annotation / article not free available online) ed.seto@moffitt.org 21-Dec-2011: Contacted by JYOTI. in vitro In vitro acetylase assay acetylase assay predetermined predetermined participant predetermined Peng L., Yuan Z., Ling H., Fukasawa K., Robertson K., Olashaw N., Koomen J., Chen J., Lane W.S., Seto E. Apoptosis - Interactions involving proteins with a function related to apoptosis Origin of GFP-SIRT1 constructs as human according sequence length (fig 3). Origin of GST-SIRT1 (fig 2) as human according pmid 12006491 Origin of Flag-PCAF (fig 1) as human according 8684459 (as in UniProt annotation / article not free available online) ed.seto@moffitt.org Accepted 2011-OCT-18 by JYOTI Mol. Cell. Biol. (0270-7306) 2011 mimix curation kat2b_human Histone acetyltransferase KAT2B P300/CBP-associated factor PCAF Lysine acetyltransferase 2B Histone acetyltransferase PCAF KAT2B Spermidine acetyltransferase KAT2B protein protein human Homo sapiens Human MSEAGGAGPGGCGAGAGAGAGPGALPPQPAALPPAPPQGSPCAAAAGGSGACGPATAVAAAGTAEGPGGGGSARIAVKKAQLRSAPRAKKLEKLGVYSACKAEESCKCNGWKNPNPSPTPPRADLQQIIVSLTESCRSCSHALAAHVSHLENVSEEEMNRLLGIVLDVEYLFTCVHKEEDADTKQVYFYLFKLLRKSILQRGKPVVEGSLEKKPPFEKPSIEQGVNNFVQYKFSHLPAKERQTIVELAKMFLNRINYWHLEAPSQRRLRSPNDDISGYKENYTRWLCYCNVPQFCDSLPRYETTQVFGRTLLRSVFTVMRRQLLEQARQEKDKLPLEKRTLILTHFPKFLSMLEEEVYSQNSPIWDQDFLSASSRTSQLGIQTVINPPPVAGTISYNSTSSSLEQPNAGSSSPACKASSGLEANPGEKRKMTDSHVLEEAKKPRVMGDIPMELINEVMSTITDPAAMLGPETNFLSAHSARDEAARLEERRGVIEFHVVGNSLNQKPNKKILMWLVGLQNVFSHQLPRMPKEYITRLVFDPKHKTLALIKDGRVIGGICFRMFPSQGFTEIVFCAVTSNEQVKGYGTHLMNHLKEYHIKHDILNFLTYADEYAIGYFKKQGFSKEIKIPKTKYVGYIKDYEGATLMGCELNPRIPYTEFSVIIKKQKEIIKKLIERKQAQIRKVYPGLSCFKDGVRQIPIESIPGIRETGWKPSGKEKSKEPRDPDQLYSTLKSILQQVKSHQSAWPFMEPVKRTEAPGYYEVIRFPMDLKTMSERLKNRYYVSKKLFMADLQRVFTNCKEYNPPESEYYKCANILEKFFFSKIKEAGLIDK DIP protein Q92831 original sequence version: 159 DIP protein Q92831 original sequence version: 153 DIP protein Q92831 original sequence version: 84 72F516E8BC00CC0C sir1_human NAD-dependent protein deacetylase sirtuin-1 NAD-dependent protein deacylase sirtuin-1 SIRT1 SIR2L1 SIR2-like protein 1 Regulatory protein SIR2 homolog 1 protein protein human Homo sapiens Human MADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGLERSPGEPGGAAPEREVPAAARGCPGAAAAALWREAEAEAAAAGGEQEAQATAAAGEGDNGPGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSCESDEEDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS DIP protein Q96EB6 original sequence version: 140 DIP protein Q96EB6 original sequence version: 71 DIP protein Q96EB6 original sequence version: 125 DIP protein Q96EB6 original sequence version: 115 DIP protein Q96EB6 original sequence version: 63 DIP protein Q96EB6-1 original sequence version: 102 DIP protein Q96EB6 original sequence version: 102 DIP protein Q96EB6 original sequence version: 98 DIP protein Q96EB6 original sequence version: 144 DIP protein Q96EB6 original sequence version: 120 DIP protein Q96EB6-1 original sequence version: 120 DIP protein Q96EB6 original sequence version: 113 DIP protein Q96EB6-1 original sequence version: 117 2D3BEA6D73DA229F dnmt1_human DNA (cytosine-5)-methyltransferase 1 CXXC9 MCMT DNA methyltransferase HsaI CXXC-type zinc finger protein 9 DNMT1 AIM DNMT protein protein human Homo sapiens Human MPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKPEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGVQADEDEDGDEKDEKKHRSQPKDLAAKRRPEEKEPEKVNPQISDEKDEDEKEEKRRKTTPKEPTEKKMARAKTVMNSKTHPPKCIQCGQYLDDPDLKYGQHPPDAVDEPQMLTNEKLSIFDANESGFESYEALPQHKLTCFSVYCKHGHLCPIDTGLIEKNIELFFSGSAKPIYDDDPSLEGGVNGKNLGPINEWWITGFDGGEKALIGFSTSFAEYILMDPSPEYAPIFGLMQEKIYISKIVVEFLQSNSDSTYEDLINKIETTVPPSGLNLNRFTEDSLLRHAQFVVEQVESYDEAGDSDEQPIFLTPCMRDLIKLAGVTLGQRRAQARRQTIRHSTREKDRGPTKATTTKLVYQIFDTFFAEQIEKDDREDKENAFKRRRCGVCEVCQQPECGKCKACKDMVKFGGSGRSKQACQERRCPNMAMKEADDDEEVDDNIPEMPSPKKMHQGKKKKQNKNRISWVGEAVKTDGKKSYYKKVCIDAETLEVGDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNKGKGKGKGKGKPKSQACEPSEPEIEIKLPKLRTLDVFSGCGGLSEGFHQAGISDTLWAIEMWDPAAQAFRLNNPGSTVFTEDCNILLKLVMAGETTNSRGQRLPQKGDVEMLCGGPPCQGFSGMNRFNSRTYSKFKNSLVVSFLSYCDYYRPRFFLLENVRNFVSFKRSMVLKLTLRCLVRMGYQCTFGVLQAGQYGVAQTRRRAIILAAAPGEKLPLFPEPLHVFAPRACQLSVVVDDKKFVSNITRLSSGPFRTITVRDTMSDLPEVRNGASALEISYNGEPQSWFQRQLRGAQYQPILRDHICKDMSALVAARMRHIPLAPGSDWRDLPNIEVRLSDGTMARKLRYTHHDRKNGRSSSGALRGVCSCVEAGKACDPAARQFNTLIPWCLPHTGNRHNHWAGLYGRLEWDGFFSTTVTNPEPMGKQGRVLHPEQHRVVSVRECARSQGFPDTYRLFGNILDKHRQVGNAVPPPLAKAIGLEIKLCMLAKARESASAKIKEEEAAKD DIP protein P26358 original sequence version: 171 DIP protein P26358-1 original sequence version: 121 DIP protein P26358 original sequence version: 121 DIP protein P26358-1 original sequence version: 128 DIP protein P26358 original sequence version: 128 1E833192D22AFA5B dnmt1-kat2b 5 8 enzyme target enzyme target substrate substrate neutral component neutral component 6 enzyme enzyme neutral component neutral component direct interaction direct interaction 1 dnmt1-sirt1 3 8 enzyme target enzyme target substrate substrate neutral component neutral component 7 enzyme enzyme neutral component neutral component direct interaction direct interaction 2 dnmt1-sirt1-1 1 8 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization 3a dnmt1-sirt1-2 4 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 3b, c and d sirt1-dnmt1 4 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 3b dnmt1-sirt1-3 2 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 3b sirt1-dnmt1-1 2 8 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 3b