IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Activation of mTORC2 by association with the ribosome. Activation of mTORC2 by association with the ribosome. Cell (0092-8674) 2011 imex curation Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. m.hall@unibas.ch 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions human-juso Homo sapiens juso Human melanoma cell line Juso density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. 15%–45% sucrose gradient m.hall@unibas.ch Accepted 2011-AUG-31 by MGT 23-Sept-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation Activation of mTORC2 by association with the ribosome. Activation of mTORC2 by association with the ribosome. Cell (0092-8674) 2011 imex curation Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. m.hall@unibas.ch 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. 15%–45% sucrose gradient m.hall@unibas.ch Accepted 2011-AUG-31 by MGT 23-Sept-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation Activation of mTORC2 by association with the ribosome. Activation of mTORC2 by association with the ribosome. Cell (0092-8674) 2011 imex curation Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. m.hall@unibas.ch 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions human-sw620 Human caucasian colon adenocarcinoma SW620 cells sw620 Human caucasian colon adenocarcinoma SW620 cells density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. 15%–45% sucrose gradient m.hall@unibas.ch Accepted 2011-AUG-31 by MGT 23-Sept-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation Activation of mTORC2 by association with the ribosome. Activation of mTORC2 by association with the ribosome. Cell (0092-8674) 2011 imex curation Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. m.hall@unibas.ch 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions human-ht_29 Homo sapiens HT-29 caucasian women with colon adenocarcinoma ht_29 HT-29 confirmed as human with IEF of AST, LDH established from the primary tumor of a 44-year-old Caucasian woman with colon adenocarcinoma in 1964; described to be heterotransplantable forming well-differentiated grade I tumors density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. 15%–45% sucrose gradient m.hall@unibas.ch Accepted 2011-AUG-31 by MGT 23-Sept-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation Activation of mTORC2 by association with the ribosome. Activation of mTORC2 by association with the ribosome. Cell (0092-8674) 2011 imex curation Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. m.hall@unibas.ch 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. m.hall@unibas.ch Accepted 2011-AUG-31 by MGT 23-Sept-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation Activation of mTORC2 by association with the ribosome. Activation of mTORC2 by association with the ribosome. Cell (0092-8674) 2011 imex curation Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. m.hall@unibas.ch 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions human-me67 Homo sapiens me67 Human melanom cell line ME67 density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. 15%–45% sucrose gradient m.hall@unibas.ch Accepted 2011-AUG-31 by MGT 23-Sept-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation Activation of mTORC2 by association with the ribosome. Activation of mTORC2 by association with the ribosome. Cell (0092-8674) 2011 imex curation Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. m.hall@unibas.ch 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. m.hall@unibas.ch Accepted 2011-AUG-31 by MGT 23-Sept-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation Activation of mTORC2 by association with the ribosome. Activation of mTORC2 by association with the ribosome. Cell (0092-8674) 2011 imex curation Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. m.hall@unibas.ch 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions human-na8 Homo sapiens na8 Human melanoma cell line NA8 density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. 15%–45% sucrose gradient m.hall@unibas.ch Accepted 2011-AUG-31 by MGT 23-Sept-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation Activation of mTORC2 by association with the ribosome. Activation of mTORC2 by association with the ribosome. Cell (0092-8674) 2011 imex curation Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. m.hall@unibas.ch 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. m.hall@unibas.ch Accepted 2011-AUG-31 by MGT 23-Sept-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation Activation of mTORC2 by association with the ribosome. Activation of mTORC2 by association with the ribosome. Cell (0092-8674) 2011 imex curation Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. m.hall@unibas.ch 23-Sept-2011: Contacted by JYOTI. mgt Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Zinzalla V., Stracka D., Oppliger W., Hall MN. Apoptosis - Interactions involving proteins with a function related to apoptosis Fig. 1D not annotated as enzyme is not identified - TOR2 would IP with AVO3 and is the active molecule. 15%–45% sucrose gradient m.hall@unibas.ch Accepted 2011-AUG-31 by MGT 23-Sept-2011: Contacted by JYOTI. Cell (0092-8674) 2011 mgt Only protein-protein interactions imex curation rl7_human 60S ribosomal protein L7 RPL7 Large ribosomal subunit protein uL30 protein protein human Homo sapiens Human MEGVEEKKKEVPAVPETLKKKRRNFAELKIKRLRKKFAQKMLRKARRKLIYEKAKHYHKEYRQMYRTEIRMARMARKAGNFYVPAEPKLAFVIRIRGINGVSPKVRKVLQLLRLRQIFNGTFVKLNKASINMLRIVEPYIAWGYPNLKSVNELIYKRGYGKINKKRIALTDNALIARSLGKYGIICMEDLIHEIYTVGKRFKEANNFLWPFKLSSPRGGMKKKTTHFVEGGDAGNREDQINRLIRRMN 070EB4C904E6795A mtor_human Serine/threonine-protein kinase mTOR MTOR FRAP FRAP2 FK506-binding protein 12-rapamycin complex-associated protein 1 Rapamycin target protein 1 FRAP1 Mammalian target of rapamycin FKBP12-rapamycin complex-associated protein Mechanistic target of rapamycin RAFT1 RAPT1 Rapamycin and FKBP12 target 1 protein protein human Homo sapiens Human MLGTGPAAATTAATTSSNVSVLQQFASGLKSRNEETRAKAAKELQHYVTMELREMSQEESTRFYDQLNHHIFELVSSSDANERKGGILAIASLIGVEGGNATRIGRFANYLRNLLPSNDPVVMEMASKAIGRLAMAGDTFTAEYVEFEVKRALEWLGADRNEGRRHAAVLVLRELAISVPTFFFQQVQPFFDNIFVAVWDPKQAIREGAVAALRACLILTTQREPKEMQKPQWYRHTFEEAEKGFDETLAKEKGMNRDDRIHGALLILNELVRISSMEGERLREEMEEITQQQLVHDKYCKDLMGFGTKPRHITPFTSFQAVQPQQSNALVGLLGYSSHQGLMGFGTSPSPAKSTLVESRCCRDLMEEKFDQVCQWVLKCRNSKNSLIQMTILNLLPRLAAFRPSAFTDTQYLQDTMNHVLSCVKKEKERTAAFQALGLLSVAVRSEFKVYLPRVLDIIRAALPPKDFAHKRQKAMQVDATVFTCISMLARAMGPGIQQDIKELLEPMLAVGLSPALTAVLYDLSRQIPQLKKDIQDGLLKMLSLVLMHKPLRHPGMPKGLAHQLASPGLTTLPEASDVGSITLALRTLGSFEFEGHSLTQFVRHCADHFLNSEHKEIRMEAARTCSRLLTPSIHLISGHAHVVSQTAVQVVADVLSKLLVVGITDPDPDIRYCVLASLDERFDAHLAQAENLQALFVALNDQVFEIRELAICTVGRLSSMNPAFVMPFLRKMLIQILTELEHSGIGRIKEQSARMLGHLVSNAPRLIRPYMEPILKALILKLKDPDPDPNPGVINNVLATIGELAQVSGLEMRKWVDELFIIIMDMLQDSSLLAKRQVALWTLGQLVASTGYVVEPYRKYPTLLEVLLNFLKTEQNQGTRREAIRVLGLLGALDPYKHKVNIGMIDQSRDASAVSLSESKSSQDSSDYSTSEMLVNMGNLPLDEFYPAVSMVALMRIFRDQSLSHHHTMVVQAITFIFKSLGLKCVQFLPQVMPTFLNVIRVCDGAIREFLFQQLGMLVSFVKSHIRPYMDEIVTLMREFWVMNTSIQSTIILLIEQIVVALGGEFKLYLPQLIPHMLRVFMHDNSPGRIVSIKLLAAIQLFGANLDDYLHLLLPPIVKLFDAPEAPLPSRKAALETVDRLTESLDFTDYASRIIHPIVRTLDQSPELRSTAMDTLSSLVFQLGKKYQIFIPMVNKVLVRHRINHQRYDVLICRIVKGYTLADEEEDPLIYQHRMLRSGQGDALASGPVETGPMKKLHVSTINLQKAWGAARRVSKDDWLEWLRRLSLELLKDSSSPSLRSCWALAQAYNPMARDLFNAAFVSCWSELNEDQQDELIRSIELALTSQDIAEVTQTLLNLAEFMEHSDKGPLPLRDDNGIVLLGERAAKCRAYAKALHYKELEFQKGPTPAILESLISINNKLQQPEAAAGVLEYAMKHFGELEIQATWYEKLHEWEDALVAYDKKMDTNKDDPELMLGRMRCLEALGEWGQLHQQCCEKWTLVNDETQAKMARMAAAAAWGLGQWDSMEEYTCMIPRDTHDGAFYRAVLALHQDLFSLAQQCIDKARDLLDAELTAMAGESYSRAYGAMVSCHMLSELEEVIQYKLVPERREIIRQIWWERLQGCQRIVEDWQKILMVRSLVVSPHEDMRTWLKYASLCGKSGRLALAHKTLVLLLGVDPSRQLDHPLPTVHPQVTYAYMKNMWKSARKIDAFQHMQHFVQTMQQQAQHAIATEDQQHKQELHKLMARCFLKLGEWQLNLQGINESTIPKVLQYYSAATEHDRSWYKAWHAWAVMNFEAVLHYKHQNQARDEKKKLRHASGANITNATTAATTAATATTTASTEGSNSESEAESTENSPTPSPLQKKVTEDLSKTLLMYTVPAVQGFFRSISLSRGNNLQDTLRVLTLWFDYGHWPDVNEALVEGVKAIQIDTWLQVIPQLIARIDTPRPLVGRLIHQLLTDIGRYHPQALIYPLTVASKSTTTARHNAANKILKNMCEHSNTLVQQAMMVSEELIRVAILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQLPQLTSLELQYVSPKLLMCRDLELAVPGTYDPNQPIIRIQSIAPSLQVITSKQRPRKLTLMGSNGHEFVFLLKGHEDLRQDERVMQLFGLVNTLLANDPTSLRKNLSIQRYAVIPLSTNSGLIGWVPHCDTLHALIRDYREKKKILLNIEHRIMLRMAPDYDHLTLMQKVEVFEHAVNNTAGDDLAKLLWLKSPSSEVWFDRRTNYTRSLAVMSMVGYILGLGDRHPSNLMLDRLSGKILHIDFGDCFEVAMTREKFPEKIPFRLTRMLTNAMEVTGLDGNYRITCHTVMEVLREHKDSVMAVLEAFVYDPLLNWRLMDTNTKGNKRSRTRTDSYSAGQSVEILDGVELGEPAHKKTGTTVPESIHSFIGDGLVKPEALNKKAIQIINRVRDKLTGRDFSHDDTLDVPTQVELLIKQATSHENLCQCYIGWCPFW DIP protein P42345 original sequence version: 158 DIP protein P42345 original sequence version: 82 DIP protein P42345 original sequence version: 108 DIP protein P42345 original sequence version: 107 DIP protein P42345 original sequence version: 138 DIP protein P42345 original sequence version: 168 DIP protein P42345 original sequence version: 173 DIP protein P42345 original sequence version: 120 DIP protein P42345 original sequence version: 149 DIP protein P42345 original sequence version: 134 DIP protein P42345 original sequence version: 148 DIP protein P42345 original sequence version: 118 7D9AD6E784882AB4 rs16_human 40S ribosomal protein S16 RPS16 Small ribosomal subunit protein uS9 protein protein human Homo sapiens Human MPSKGPLQSVQVFGRKKTATAVAHCKRGNGLIKVNGRPLEMIEPRTLQYKLLEPVLLLGKERFAGVDIRVRVKGGGHVAQIYAIRQSISKALVAYYQKYVDEASKKEIKDILIQYDRTLLVADPRRCESKKFGGPGARARYQKSYR DIP protein P62249 original sequence version: 115 519BCFB91BB68A15 dt-dna oligo(dT) dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid chemical synthesis Chemical synthesis (Chemical synthesis) rl26_human 60S ribosomal protein L26 RPL26 Large ribosomal subunit protein uL24 protein protein human Homo sapiens Human MKFNPFVTSDRSKNRKRHFNAPSHIRRKIMSSPLSKELRQKYNVRSMPIRKDDEVQVVRGHYKGQQIGKVVQVYRKKYVIYIERVQREKANGTTVHVGIHPSKVVITRLKLDKDRKKILERKAKSRQVGKEKGKYKEETIEKMQE DIP protein P61254 original sequence version: 113 049D53AB4E3F46B6 lst8_human Target of rapamycin complex subunit LST8 MLST8 GBL G protein beta subunit-like LST8 Mammalian lethal with SEC13 protein 8 protein protein human Homo sapiens Human MNTSPGTVGSDPVILATAGYDHTVRFWQAHSGICTRTVQHQDSQVNALEVTPDRSMIAAAGYQHIRMYDLNSNNPNPIISYDGVNKNIASVGFHEDGRWMYTGGEDCTARIWDLRSRNLQCQRIFQVNAPINCVCLHPNQAELIVGDQSGAIHIWDLKTDHNEQLIPEPEVSITSAHIDPDASYMAAVNSTGNCYVWNLTGGIGDEVTQLIPKTKIPAHTRYALQCRFSPDSTLLATCSADQTCKIWRTSNFSLMTELSIKSGNPGESSRGWMWGCAFSGDSQYIVTASSDNLARLWCVETGEIKREYGGHQKAVVCLAFNDSVLG 43A600D4EF2B6543 crosstide Crosstide Ser/Thr protein kinase substrate peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) GRPRTSSFAEG rictr_human Rapamycin-insensitive companion of mTOR RICTOR KIAA1999 AVO3 homolog protein protein human Homo sapiens Human MAAIGRGRSLKNLRVRGRNDSGEENVPLDLTREPSDNLREILQNVARLQGVSNMRKLGHLNNFTKLLCDIGHSEEKLGFHYEDIIICLRLALLNEAKEVRAAGLRALRYLIQDSSILQKVLKLKVDYLIARCIDIQQSNEVERTQALRLVRKMITVNASLFPSSVTNSLIAVGNDGLQERDRMVRACIAIICELALQNPEVVALRGGLNTILKNVIDCQLSRINEALITTILHLLNHPKTRQYVRADVELERILAPYTDFHYRHSPDTAEGQLKEDREARFLASKMGIIATFRSWAGIINLCKPGNSGIQSLIGVLCIPNMEIRRGLLEVLYDIFRLPLPVVTEEFIEALLSVDPGRFQDSWRLSDGFVAAEAKTILPHRARSRPDLMDNYLALILSAFIRNGLLEGLVEVITNSDDHISVRATILLGELLHMANTILPHSHSHHLHCLPTLMNMAASFDIPKEKRLRASAALNCLKRFHEMKKRGPKPYSLHLDHIIQKAIATHQKRDQYLRVQKDIFILKDTEEALLINLRDSQVLQHKENLEWNWNLIGTILKWPNVNLRNYKDEQLHRFVRRLLYFYKPSSKLYANLDLDFAKAKQLTVVGCQFTEFLLESEEDGQGYLEDLVKDIVQWLNASSGMKPERSLQNNGLLTTLSQHYFLFIGTLSCHPHGVKMLEKCSVFQCLLNLCSLKNQDHLLKLTVSSLDYSRDGLARVILSKILTAATDACRLYATKHLRVLLRANVEFFNNWGIELLVTQLHDKNKTISSEALDILDEACEDKANLHALIQMKPALSHLGDKGLLLLLRFLSIPKGFSYLNERGYVAKQLEKWHREYNSKYVDLIEEQLNEALTTYRKPVDGDNYVRRSNQRLQRPHVYLPIHLYGQLVHHKTGCHLLEVQNIITELCRNVRTPDLDKWEEIKKLKASLWALGNIGSSNWGLNLLQEENVIPDILKLAKQCEVLSIRGTCVYVLGLIAKTKQGCDILKCHNWDAVRHSRKHLWPVVPDDVEQLCNELSSIPSTLSLNSESTSSRHNSESESVPSSMFILEDDRFGSSSTSTFFLDINEDTEPTFYDRSGPIKDKNSFPFFASSKLVKNRILNSLTLPNKKHRSSSDPKGGKLSSESKTSNRRIRTLTEPSVDFNHSDDFTPISTVQKTLQLETSFMGNKHIEDTGSTPSIGENDLKFTKNFGTENHRENTSRERLVVESSTSSHMKIRSQSFNTDTTTSGISSMSSSPSRETVGVDATTMDTDCGSMSTVVSTKTIKTSHYLTPQSNHLSLSKSNSVSLVPPGSSHTLPRRAQSLKAPSIATIKSLADCNFSYTSSRDAFGYATLKRLQQQRMHPSLSHSEALASPAKDVLFTDTITMKANSFESRLTPSRFMKALSYASLDKEDLLSPINQNTLQRSSSVRSMVSSATYGGSDDYIGLALPVDINDIFQVKDIPYFQTKNIPPHDDRGARAFAHDAGGLPSGTGGLVKNSFHLLRQQMSLTEIMNSIHSDASLFLESTEDTGLQEHTDDNCLYCVCIEILGFQPSNQLSAICSHSDFQDIPYSDWCEQTIHNPLEVVPSKFSGISGCSDGVSQEGSASSTKSTELLLGVKTIPDDTPMCRILLRKEVLRLVINLSSSVSTKCHETGLLTIKEKYPQTFDDICLYSEVSHLLSHCTFRLPCRRFIQELFQDVQFLQMHEEAEAVLATPPKQPIVDTSAES DIP protein Q6R327 original sequence version: 116 DIP protein Q6R327 original sequence version: 81 DIP protein Q6R327 original sequence version: 93 DB7B1E4A45DAE2AB sin1_human Target of rapamycin complex 2 subunit MAPKAP1 MIP1 Mitogen-activated protein kinase 2-associated protein 1 Stress-activated map kinase-interacting protein 1 MAPKAP1 SIN1 protein protein human Homo sapiens Human MAFLDNPTIILAHIRQSHVTSDDTGMCEMVLIDHDVDLEKIHPPSMPGDSGSEIQGSNGETQGYVYAQSVDITSSWDFGIRRRSNTAQRLERLRKERQNQIKCKNIQWKERNSKQSAQELKSLFEKKSLKEKPPISGKQSILSVRLEQCPLQLNNPFNEYSKFDGKGHVGTTATKKIDVYLPLHSSQDRLLPMTVVTMASARVQDLIGLICWQYTSEGREPKLNDNVSAYCLHIAEDDGEVDTDFPPLDSNEPIHKFGFSTLALVEKYSSPGLTSKESLFVRINAAHGFSLIQVDNTKVTMKEILLKAVKRRKGSQKVSGPQYRLEKQSEPNVAVDLDSTLESQSAWEFCLVRENSSRADGVFEEDSQIDIATVQDMLSSHHYKSFKVSMIHRLRFTTDVQLGISGDKVEIDPVTNQKASTKFWIKQKPISIDSDLLCACDLAEEKSPSHAIFKLTYLSNHDYKHLYFESDAATVNEIVLKVNYILESRASTARADYFAQKQRKLNRRTSFSFQKEKKSGQQ DIP protein Q9BPZ7 original sequence version: 111 E3B808C6E58F7C48 rl17_human 60S ribosomal protein L17 60S ribosomal protein L23 PD-1 RPL17 Large ribosomal subunit protein uL22 protein protein human Homo sapiens Human MVRYSLDPENPTKSCKSRGSNLRVHFKNTRETAQAIKGMHIRKATKYLKDVTLQKQCVPFRRYNGGVGRCAQAKQWGWTQGRWPKKSAEFLLHMLKNAESNAELKGLDVDSLVIEHIQVNKAPKMRRRTYRAHGRINPYMSSPCHIEMILTEKEQIVPKPEEEVAQKKKISQKKLKKQKLMARE 2FC595DD74ED1CA0 rack1_human Receptor of activated protein C kinase 1 Small ribosomal subunit protein RACK1 HLC7 PIG21 RACK1 Cell proliferation-inducing gene 21 protein GNB2L1 Guanine nucleotide-binding protein subunit beta-2-like 1 Guanine nucleotide-binding protein subunit beta-like protein 12.3 Human lung cancer oncogene 7 protein Receptor for activated C kinase protein protein human Homo sapiens Human MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR DIP protein P63244 original sequence version: 71 257F91E369ED2044 ypk2_yeast Serine/threonine-protein kinase YPK2/YKR2 YPK2 YKR2 YMR104C YM9718.03C protein protein yeast Saccharomyces cerevisiae Baker's yeast MHSWRISKFKLGRSKEDDGSSEDENEKSWGNGLFHFHHGEKHHDGSPKNHNHEHEHHIRKINTNETLPSSLSSPKLRNDASFKNPSGIGNDNSKASERKASQSSTETQGPSSESGLMTVKVYSGKDFTLPFPITSNSTILQKLLSSGILTSSSNDASEVAAIMRQLPRYKRVDQDSAGEGLIDRAFATKFIPSSILLPGSTNSSPLLYFTIEFDNSITTISPDMGTMEQPVFNKISTFDVTRKLRFLKIDVFARIPSLLLPSKNWQQEIGEQDEVLKEILKKINTNQDIHLDSFHLPLNLKIDSAAQIRLYNHHWISLERGYGKLNITVDYKPSKNKPLSIDDFDLLKVIGKGSFGKVMQVRKKDTQKIYALKALRKAYIVSKCEVTHTLAERTVLARVDCPFIVPLKFSFQSPEKLYLVLAFINGGELFYHLQHEGRFSLARSRFYIAELLCALDSLHKLDVIYRDLKPENILLDYQGHIALCDFGLCKLNMKDNDKTDTFCGTPEYLAPEILLGQGYTKTVDWWTLGILLYEMMTGLPPYYDENVPVMYKKILQQPLLFPDGFDPAAKDLLIGLLSRDPSRRLGVNGTDEIRNHPFFKDISWKKLLLKGYIPPYKPIVKSEIDTANFDQEFTKEKPIDSVVDEYLSASIQKQFGGWTYIGDEQLGDSPSQGRSIS E1FAB0295386A113 E1FAB0295386A113 crosstide-ypk2 7 22 enzyme enzyme unspecified role unspecified role region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 17 enzyme target enzyme target substrate substrate unspecified role unspecified role chemical synthesis Chemical synthesis (Chemical synthesis) phosphorylation phosphorylation reaction phosphorylation Fig. 1C rictor-mapkap1 5 19 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 18 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey association association Fig. 2B, 3B. mapkap1-mtor-1 10 15 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component colocalization colocalization Supp. Fig. 5A dt_dna-gnb2l1 9 11 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 18 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 14 unspecified role unspecified role bait bait human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 12 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 15 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 21 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 association association Supp Fig. 5B Binding assumed to be via Poly(A) mRNA-bound to ribosomes RNAse A rpl26-mtor 5 15 unspecified role unspecified role bait bait 18 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey association association Supp. Fig. 5C, S6C, S6D. Fig 6A, 6B, 6C. Inhibition of PI3K strongly decreased mTORC2/Rictor-Rpl26 interaction, conversely hyperactivation of PI3K signaling increased the amount of mTOR and rictor in Rpl26 immunoprecipitates, Insulin stimulates mTORC2-ribosome association. Insulin, PTEN siRNA LY294002 rpl26-mapkap1 5 12 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait 18 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey association association Fig. 5A rpl26-mtor-2 5 19 unspecified role unspecified role ancillary ancillary 15 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey association association Supp. Fig. 6A Inhibition of PI3K strongly decreased mTORC2/Rictor-Rpl26 interaction, conversely hyperactivation of PI3K signaling increased the amount of mTOR and rictor in Rpl26 immunoprecipitates, Insulin stimulates mTORC2-ribosome association. rpl26-mtor-5 5 20 unspecified role unspecified role ancillary ancillary 18 unspecified role unspecified role prey prey 13 unspecified role unspecified role ancillary ancillary 15 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey association association Supp. Fig. 6B mapkap1-mtor-2 10 11 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 5B gnb2l1-mapkap1 8 12 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component 21 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 7B gnb2l1-mapkap1-1 1 15 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component 21 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 7B gnb2l1-mapkap1-3 4 15 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component 21 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. S7A gnb2l1-mapkap1-3 6 11 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 21 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 7B gnb2l1-mapkap1-5 3 21 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component colocalization colocalization Supp. Fig. 7A gnb2l1-mapkap1-6 2 19 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 21 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component colocalization colocalization Supp. Fig. 7A