Molecular Connections Molecular Connections Pvt. Ltd. 2/2, Kariappa Road, Basavanagudi, Bangalore -560 004 www.molecularconnections.com PIAS1 interacts with FLASH and enhances its co-activation of c-Myb. PIAS1 interacts with FLASH and enhances its co-activation of c-Myb. Mol. Cancer (null) 2011 imex curation Alm-Kristiansen AH., Lorenzo PI., Molværsmyr AK., Matre V., Ledsaak M., Sæther T., Gabrielsen OS. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer o.s.gabrielsen@imbv.uio.no. 23-Sept-2011: Contacted by JYOTI. Human bone marrow cDNA orchard Only protein-protein interactions Curation by: Molecular Connections Pvt. Ltd yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Alm-Kristiansen AH., Lorenzo PI., Molværsmyr AK., Matre V., Ledsaak M., Sæther T., Gabrielsen OS. Apoptosis - Interactions involving proteins with a function related to apoptosis Y2H-mating assay where bait and prey plasmids were retransformed in AH109 and Y187 respectively, subjected to mating and subsequent reporter activation testing. o.s.gabrielsen@imbv.uio.no. Accepted 2011-SEP-12 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Human bone marrow cDNA Mol. Cancer (null) 2011 orchard Only protein-protein interactions imex curation Curation by: Molecular Connections Pvt. Ltd PIAS1 interacts with FLASH and enhances its co-activation of c-Myb. PIAS1 interacts with FLASH and enhances its co-activation of c-Myb. Mol. Cancer (null) 2011 imex curation Alm-Kristiansen AH., Lorenzo PI., Molværsmyr AK., Matre V., Ledsaak M., Sæther T., Gabrielsen OS. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer o.s.gabrielsen@imbv.uio.no. 23-Sept-2011: Contacted by JYOTI. Human bone marrow cDNA orchard Only protein-protein interactions Curation by: Molecular Connections Pvt. Ltd in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Alm-Kristiansen AH., Lorenzo PI., Molværsmyr AK., Matre V., Ledsaak M., Sæther T., Gabrielsen OS. Apoptosis - Interactions involving proteins with a function related to apoptosis o.s.gabrielsen@imbv.uio.no. Accepted 2011-SEP-12 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Human bone marrow cDNA Mol. Cancer (null) 2011 orchard Only protein-protein interactions imex curation Curation by: Molecular Connections Pvt. Ltd PIAS1 interacts with FLASH and enhances its co-activation of c-Myb. PIAS1 interacts with FLASH and enhances its co-activation of c-Myb. Mol. Cancer (null) 2011 imex curation Alm-Kristiansen AH., Lorenzo PI., Molværsmyr AK., Matre V., Ledsaak M., Sæther T., Gabrielsen OS. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer o.s.gabrielsen@imbv.uio.no. 23-Sept-2011: Contacted by JYOTI. Human bone marrow cDNA orchard Only protein-protein interactions Curation by: Molecular Connections Pvt. Ltd yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Alm-Kristiansen AH., Lorenzo PI., Molværsmyr AK., Matre V., Ledsaak M., Sæther T., Gabrielsen OS. Apoptosis - Interactions involving proteins with a function related to apoptosis o.s.gabrielsen@imbv.uio.no. Accepted 2011-SEP-12 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Human bone marrow cDNA Mol. Cancer (null) 2011 orchard Only protein-protein interactions imex curation Curation by: Molecular Connections Pvt. Ltd PIAS1 interacts with FLASH and enhances its co-activation of c-Myb. PIAS1 interacts with FLASH and enhances its co-activation of c-Myb. Mol. Cancer (null) 2011 imex curation Alm-Kristiansen AH., Lorenzo PI., Molværsmyr AK., Matre V., Ledsaak M., Sæther T., Gabrielsen OS. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer o.s.gabrielsen@imbv.uio.no. 23-Sept-2011: Contacted by JYOTI. Human bone marrow cDNA orchard Only protein-protein interactions Curation by: Molecular Connections Pvt. Ltd chlae-cv-1 Monkey African Green kidney fibroblast Grivet cv-1 Monkey African Green kidney fibroblast confocal microscopy confocal microscopy immunostaining immunostaining Alm-Kristiansen AH., Lorenzo PI., Molværsmyr AK., Matre V., Ledsaak M., Sæther T., Gabrielsen OS. Apoptosis - Interactions involving proteins with a function related to apoptosis o.s.gabrielsen@imbv.uio.no. Accepted 2011-SEP-12 by ORCHARD 23-Sept-2011: Contacted by JYOTI. Human bone marrow cDNA Mol. Cancer (null) 2011 orchard Only protein-protein interactions imex curation Curation by: Molecular Connections Pvt. Ltd myb_human Transcriptional activator Myb MYB Proto-oncogene c-Myb protein protein human Homo sapiens Human MARRPRHSIYSSDEDDEDFEMCDHDYDGLLPKSGKRHLGKTRWTREEDEKLKKLVEQNGTDDWKVIANYLPNRTDVQCQHRWQKVLNPELIKGPWTKEEDQRVIELVQKYGPKRWSVIAKHLKGRIGKQCRERWHNHLNPEVKKTSWTEEEDRIIYQAHKRLGNRWAEIAKLLPGRTDNAIKNHWNSTMRRKVEQEGYLQESSKASQPAVATSFQKNSHLMGFAQAPPTAQLPATGQPTVNNDYSYYHISEAQNVSSHVPYPVALHVNIVNVPQPAAAAIQRHYNDEDPEKEKRIKELELLLMSTENELKGQQVLPTQNHTCSYPGWHSTTIADHTRPHGDSAPVSCLGEHHSTPSLPADPGSLPEESASPARCMIVHQGTILDNVKNLLEFAETLQFIDSFLNTSSNHENSDLEMPSLTSTPLIGHKLTVTTPFHRDQTVKTQKENTVFRTPAIKRSILESSPRTPTPFKHALAAQEIKYGPLKMLPQTPSHLVEDLQDVIKQESDESGIVAEFQENGPPLLKKIKQEVESPTDKSGNFFCSHHWEGDSLNTQLFTQTSPVADAPNILTSSVLMAPASEDEDNVLKAFTVPKNRSLASPLQPCSSTWEPASCGKMEEQMTSSSQARKYVNAFSARTLVM DIP protein P10242 original sequence version: 144 DIP protein P10242 original sequence version: 133 DIP protein P10242 original sequence version: 157 FD5E6694020426ED c8ap2_human CASP8-associated protein 2 CASP8AP2 FLASH KIAA1315 RIP25 FLICE-associated huge protein protein protein human Homo sapiens Human MAADDDNGDGTSLFDVFSASPLKNNDEGSLDIYAGLDSAVSDSASKSCVPSRNCLDLYEEILTEEGTAKEATYNDLQVEYGKCQLQMKELMKKFKEIQTQNFSLINENQSLKKNISALIKTARVEINRKDEEISNLHQRLSEFPHFRNNHKTARTFDTVKTKDLKSRSPHLDDCSKTDHRAKSDVSKDVHHSTSLPNLEKEGKPHSDKRSTSHLPTSVEKHCTNGVWSRSHYQVGEGSSNEDSRRGRKDIRHSQFNRGTERVRKDLSTGCGDGEPRILEASQRLQGHPEKYGKGEPKTESKSSKFKSNSDSDYKGERINSSWEKETPGERSHSRVDSQSDKKLERQSERSQNINRKEVKSQDKEERKVDQKPKSVVKDQDHWRRSERASLPHSKNEITFSHNSSKYHLEERRGWEDCKRDKSVNSHSFQDGRCPSSLSNSRTHKNIDSKEVDAMHQWENTPLKAERHRTEDKRKREQESKEENRHIRNEKRVPTEHLQKTNKETKKTTTDLKKQNEPKTDKGEVLDNGVSEGADNKELAMKAESGPNETKNKDLKLSFMKKLNLTLSPAKKQPVSQDNQHKITDIPKSSGVCDSESSMQVKTVAYVPSISEHILGEAAVSEHTMGETKSTLLEPKVALLAVTEPRIGISETNKEDENSLLVRSVDNTMHCEEPICGTETSFPSPMEIQQTESLFPSTGMKQTINNGRAAAPVVMDVLQTDVSQNFGLELDTKRNDNSDYCGISEGMEMKVALSTTVSETTESILQPSIEEADILPIMLSEDNNPKFEPSVIVTPLVESKSCHLEPCLPKETLDSSLQQTELMDHRMATGETNSVYHDDDNSVLSIDLNHLRPIPEAISPLNSPVRPVAKVLRNESPPQVPVYNNSHKDVFLPNSAHSTSKSQSDLNKENQKPIYKSDKCTEADTCKNSPLDELEEGEIRSDSETSKPQESFEKNSKRRVSADVRKSKTIPRRGKSTVCLDKDSRKTHVRIHQTNNKWNKRPDKSSRSSKTEKKDKVMSTSSLEKIVPIIAVPSSEQEIMHMLRMIRKHVRKNYMKFKAKFSLIQFHRIIESAILSFTSLIKHLNLHKISKSVTTLQKNLCDIIESKLKQVKKNGIVDRLFEQQLPDMKKKLWKFVDDQLDYLFAKLKKILVCDSKSFGRDSDEGKLEKTSKQNAQYSNSQKRSVDNSNRELLKEKLSKSEDPVHYKSLVGCKKSEENYQDQNNSSINTVKHDIKKNFNICFDNIKNSQSEERSLEVHCPSTPKSEKNEGSSIEDAQTSQHATLKPERSFEILTEQQASSLTFNLVSDAQMGEIFKSLLQGSDLLDSSVNCTEKSEWELKTPEKQLLETLKCESIPACTTEELVSGVASPCPKMISDDNWSLLSSEKGPSLSSGLSLPVHPDVLDESCMFEVSTNLPLSKDNVCSVEKSKPCVSSILLEDLAVSLTVPSPLKSDGHLSFLKPDMSSSSTPEEVISAHFSEDALLEEEDASEQDIHLALESDNSSSKSSCSSSWTSRSVAPGFQYHPNLPMHAVIMEKSNDHFIVKIRRATPSTSSGLKQSMMPDELLTSLPRHGKEADEGPEKEYISCQNTVFKSVEELENSNKNVDGSKSTHEEQSSMIQTQVPDIYEFLKDASDKMGHSDEVADECFKLHQVWETKVPESIEELPSMEEISHSVGEHLPNTYVDLTKDPVTETKNLGEFIEVTVLHIDQLGCSGGNLNQSAQILDNSLQADTVGAFIDLTQDASSEAKSEGNHPALAVEDLGCGVIQVDEDNCKEEKAQVANRPLKCIVEETYIDLTTESPSSCEVKKDELKSEPGSNCDNSELPGTLHNSHKKRRNISDLNHPHKKQRKETDLTNKEKTKKPTQDSCENTEAHQKKASKKKAPPVTKDPSSLKATPGIKDSSAALATSTSLSAKNVIKKKGEIIILWTRNDDREILLECQKRGPSFKTFAYLAAKLDKNPNQVSERFQQLMKLFEKSKCR DIP protein Q9UKL3 original sequence version: 101 AF2B1A7798C19E39 pias1_human E3 SUMO-protein ligase PIAS1 Protein inhibitor of activated STAT protein 1 Gu-binding protein RNA helicase II-binding protein DEAD/H box-binding protein 1 PIAS1 DDXBP1 E3 SUMO-protein transferase PIAS1 protein protein human Homo sapiens Human MADSAELKQMVMSLRVSELQVLLGYAGRNKHGRKHELLTKALHLLKAGCSPAVQMKIKELYRRRFPQKIMTPADLSIPNVHSSPMPATLSPSTIPQLTYDGHPASSPLLPVSLLGPKHELELPHLTSALHPVHPDIKLQKLPFYDLLDELIKPTSLASDNSQRFRETCFAFALTPQQVQQISSSMDISGTKCDFTVQVQLRFCLSETSCPQEDHFPPNLCVKVNTKPCSLPGYLPPTKNGVEPKRPSRPINITSLVRLSTTVPNTIVVSWTAEIGRNYSMAVYLVKQLSSTVLLQRLRAKGIRNPDHSRALIKEKLTADPDSEIATTSLRVSLLCPLGKMRLTIPCRALTCSHLQCFDATLYIQMNEKKPTWVCPVCDKKAPYEHLIIDGLFMEILKYCTDCDEIQFKEDGTWAPMRSKKEVQEVSASYNGVDGCLSSTLEHQVASHHQSSNKNKKVEVIDLTIDSSSDEEEEEPSAKRTCPSLSPTSPLNNKGILSLPHQASPVSRTPSLPAVDTSYINTSLIQDYRHPFHMTPMPYDLQGLDFFPFLSGDNQHYNTSLLAAAAAAVSDDQDLLHSSRFFPYTSSQMFLDQLSAGGSTSLPTTNGSSSGSNSSLVSSNSLRESHSHTVTNRSSTDTASIFGIIPDIISLD DIP protein O75925 original sequence version: 147 AA69338221124119 c8ap2_mouse CASP8-associated protein 2 Casp8ap2 Flash FLICE-associated huge protein protein protein mouse Mus musculus Mouse MAADDDNGDGTGLFDVCPASPLKNNDEGSLDIYAGLDSAVSDSTARSCVSFRNCLDLYEEILTEEGTAKEATYNDLQIEYGKCQQQMKDLMKRFKEIQTQNLNLKNENQSLKKNISALIKTARVEINRKDEEINHLHQRLSEFPHFRNNHKTARTKDSQSTSPHLDDCSKTDHGVKSDVQKDVHPNTAQPNLEKEGKSHSEAQNPLHLSTGVEKHCANNVWSRSPYQVGEGNSNEDNRRGRSGTRHSQCSRGTDRTQKDLHSSCNDSEPRDKEANSRLQGHPEKHGNSEARTESKISESKSSTGMGYKSERSASSWEKETSRERPHTRVESQHDKNLEKQNERLQNMHRKELPSQDKTERKVDVKFKPAGEEQGHRGRVDRALPPHPKNDVKHYGFNKYHPEERRGREDCKRDRGMNSHGFQDRRCSSFLSSNRNSKYPHSKEVSVAHQWENTPFKAERHRTEDRRKRERENKEESRHVKSDKKSPPEHLQRTHKDTKKSTADGKRQTEPKHGKGAVSNSELSKGTDSKEGATKVESGPNEAKGKDLKLSFMEKLNLTLSPAKKQPACQDNPHQITGVPEPSGTCDSRSLETTGTVACLPSGSEHNREETKSELPEPKEALLATSQLRISIPENKMKEEKRLLFKSVENTVPCELLACGTEISLPAPVEIEQARCLLGSVEVEETCGGARTAASVVMHVLPEHASEDASQELDTKRHDGINACAISEGVKTKVILSPKAAAASESHLAPLVEEPSISLVNCSGDNNPKLEPSLEERPIVETKSCPLESCLPKETFVPSPQKTELIDHKIETGESNSVYQDDDNSVLSIDFNNLRPIPDPISPLNSPVRPVCKVVSMESSCAIPLYDSSHKDEFPSNSTLSTFKSQSDLNKENEKPVPKFDKCSEADSCKHLSLDELEEGEIRSDDEESVAQKRLEKSARPRVSAEVQPGKSSPGSRRSTVHVHKDNGRTAVKLPRDRLTWSKRSSESRPSNTERKSKTMSISSLEKILPLILVPSSLWEVMHMLRLLGKHVRKNYMKFKIKFSLTQFHRIIESAILSFTSLVKCLDLSKICKSVSTLQKSLCEVIESNLKQVKKNGIVDRLFEQQQTDMKKKLWKFVDEQLDYLFEKLKKILLKFCDSVNFENENSEGKLGKKYKERTQHSNCQKKKMDNKEIRREKVLKSENTVNFKSSLGCEKSEEKHQDQNKTNASIVKHDVKRTFSTCSDNTKNAECKEQFLEKSCPSTPRPGKDEGHTEEEAQAAQHASAKSERSFEILTEQQASSLTFNLVSDAQMGEIFKSLLQGSDLLDTSGTEKAEWELKTPEKQLLESLKCESAPACATEELVSEGASLCPKVISDDNWSLLSSEKGPSLSSGLSLPVHPDVLDENCMFEVSSNTALGKDNVYSSEKSKPCISSILLEDLAVSLTVPSPLKSDGHLSFLKPEVLSTSTPEEVISAHFSEDALLEEEDASEQDIHLALESDNSSSKSSCSSWTSRSVASGFQYHPNLPMHAVIMEKSNDHFIVKIRRATPSTSPGLKHGVVAEESLTSLPRTGKEAGVATEKEPNLFQSTVLKPVKDLENTDKNIDKSKLTHEEQNSIVQTQVPDIYEFLKDASNKVVHCDQVVDDCFKLHQVWEPKVSENLQELPSMEKIPHSLDNHLPDTHIDLTKDSATETKSLGELMEVTVLNVDHLECSQTNLDQDAEITCSSLQPDTIDAFIDLTHDASSESKNEGSEPVLAVEGMGCQVICIDEDTNKEGKMGRANSPLESIVEETCIDLTSESPGSCEIKRHNLKSEPPSKLDCLELPETLGNGHKKRKNSPGVSHSSQKKQRKDIDLSSEKTQRLSPNSDRNGDAHRKQASKKREPAVNETSLSSEASPEVKGSTAVLAASPASLSAKNVIKKKGEIIVSWTRNDDREILLECQKRMPSLKTFTYLAVKLNKNPNQVSERFQQLKKLFEKSKCR 7B3FC637BB98D9EF flash-pias1-1 3 6 unspecified role unspecified role bait bait c-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey n-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association fig.1a flash-pias1-2 1 6 unspecified role unspecified role bait bait c-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role prey prey physical association physical association fig. 1b flash-pias1-3 1 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait n-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association fig. 1b flash-pias1-4 2 7 unspecified role unspecified role prey prey n-term flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chlae-cos_1 Chlorocebus aethiops COS-1 cells Grivet cos_1 CV-1 Simian cells (cercopithecus aethiops) transformed by SV40 6 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n-term sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association fig. 1d and fig. 2c cmyb-pias1-2 4 8 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component n-term flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization fig. 1g cmyb-pias1-3 1 5 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey c-term required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association fig. 4a