IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ RalB and the Exocyst Mediate the Cellular Starvation Response by Direct Activation of Autophagosome Assembly. RalB and the Exocyst Mediate the Cellular Starvation Response by Direct Activation of Autophagosome Assembly. Cell (0092-8674) 2011 imex curation Bodemann BO., Orvedahl A., Cheng T., Ram RR., Ou YH., Formstecher E., Maiti M., Hazelett CC., Wauson EM., Balakireva M., Camonis JH., Yeaman C., Levine B., White MA. Apoptosis - Interactions involving proteins with a function related to apoptosis Additional information on origin of constructs obtained from the author. michael.white@utsouthwestern.edu jyoti Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bodemann BO., Orvedahl A., Cheng T., Ram RR., Ou YH., Formstecher E., Maiti M., Hazelett CC., Wauson EM., Balakireva M., Camonis JH., Yeaman C., Levine B., White MA. Apoptosis - Interactions involving proteins with a function related to apoptosis Tagged molecules were visualised using tag fluorescence. Additional information on origin of constructs obtained from the author. michael.white@utsouthwestern.edu Accepted 2011-APR-19 by JYOTI Cell (0092-8674) 2011 jyoti Only protein-protein interactions imex curation RalB and the Exocyst Mediate the Cellular Starvation Response by Direct Activation of Autophagosome Assembly. RalB and the Exocyst Mediate the Cellular Starvation Response by Direct Activation of Autophagosome Assembly. Cell (0092-8674) 2011 imex curation Bodemann BO., Orvedahl A., Cheng T., Ram RR., Ou YH., Formstecher E., Maiti M., Hazelett CC., Wauson EM., Balakireva M., Camonis JH., Yeaman C., Levine B., White MA. Apoptosis - Interactions involving proteins with a function related to apoptosis Additional information on origin of constructs obtained from the author. michael.white@utsouthwestern.edu jyoti Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Bodemann BO., Orvedahl A., Cheng T., Ram RR., Ou YH., Formstecher E., Maiti M., Hazelett CC., Wauson EM., Balakireva M., Camonis JH., Yeaman C., Levine B., White MA. Apoptosis - Interactions involving proteins with a function related to apoptosis Additional information on origin of constructs obtained from the author. michael.white@utsouthwestern.edu Accepted 2011-APR-19 by JYOTI Cell (0092-8674) 2011 2011 jyoti Only protein-protein interactions imex curation RalB and the Exocyst Mediate the Cellular Starvation Response by Direct Activation of Autophagosome Assembly. RalB and the Exocyst Mediate the Cellular Starvation Response by Direct Activation of Autophagosome Assembly. Cell (0092-8674) 2011 imex curation Bodemann BO., Orvedahl A., Cheng T., Ram RR., Ou YH., Formstecher E., Maiti M., Hazelett CC., Wauson EM., Balakireva M., Camonis JH., Yeaman C., Levine B., White MA. Apoptosis - Interactions involving proteins with a function related to apoptosis Additional information on origin of constructs obtained from the author. michael.white@utsouthwestern.edu jyoti Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Bodemann BO., Orvedahl A., Cheng T., Ram RR., Ou YH., Formstecher E., Maiti M., Hazelett CC., Wauson EM., Balakireva M., Camonis JH., Yeaman C., Levine B., White MA. Apoptosis - Interactions involving proteins with a function related to apoptosis Additional information on origin of constructs obtained from the author. michael.white@utsouthwestern.edu Accepted 2011-APR-19 by JYOTI Cell (0092-8674) 2011 2011 jyoti Only protein-protein interactions imex curation RalB and the Exocyst Mediate the Cellular Starvation Response by Direct Activation of Autophagosome Assembly. RalB and the Exocyst Mediate the Cellular Starvation Response by Direct Activation of Autophagosome Assembly. Cell (0092-8674) 2011 imex curation Bodemann BO., Orvedahl A., Cheng T., Ram RR., Ou YH., Formstecher E., Maiti M., Hazelett CC., Wauson EM., Balakireva M., Camonis JH., Yeaman C., Levine B., White MA. Apoptosis - Interactions involving proteins with a function related to apoptosis Additional information on origin of constructs obtained from the author. michael.white@utsouthwestern.edu jyoti Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Bodemann BO., Orvedahl A., Cheng T., Ram RR., Ou YH., Formstecher E., Maiti M., Hazelett CC., Wauson EM., Balakireva M., Camonis JH., Yeaman C., Levine B., White MA. Apoptosis - Interactions involving proteins with a function related to apoptosis Additional information on origin of constructs obtained from the author. michael.white@utsouthwestern.edu Accepted 2011-APR-19 by JYOTI Cell (0092-8674) 2011 jyoti Only protein-protein interactions imex curation RalB and the Exocyst Mediate the Cellular Starvation Response by Direct Activation of Autophagosome Assembly. RalB and the Exocyst Mediate the Cellular Starvation Response by Direct Activation of Autophagosome Assembly. Cell (0092-8674) 2011 imex curation Bodemann BO., Orvedahl A., Cheng T., Ram RR., Ou YH., Formstecher E., Maiti M., Hazelett CC., Wauson EM., Balakireva M., Camonis JH., Yeaman C., Levine B., White MA. Apoptosis - Interactions involving proteins with a function related to apoptosis Additional information on origin of constructs obtained from the author. michael.white@utsouthwestern.edu jyoti Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Bodemann BO., Orvedahl A., Cheng T., Ram RR., Ou YH., Formstecher E., Maiti M., Hazelett CC., Wauson EM., Balakireva M., Camonis JH., Yeaman C., Levine B., White MA. Apoptosis - Interactions involving proteins with a function related to apoptosis Tagged molecules were visualised using tag fluorescence. Additional information on origin of constructs obtained from the author. michael.white@utsouthwestern.edu Accepted 2011-APR-19 by JYOTI Cell (0092-8674) 2011 jyoti Only protein-protein interactions imex curation RalB and the Exocyst Mediate the Cellular Starvation Response by Direct Activation of Autophagosome Assembly. RalB and the Exocyst Mediate the Cellular Starvation Response by Direct Activation of Autophagosome Assembly. Cell (0092-8674) 2011 imex curation Bodemann BO., Orvedahl A., Cheng T., Ram RR., Ou YH., Formstecher E., Maiti M., Hazelett CC., Wauson EM., Balakireva M., Camonis JH., Yeaman C., Levine B., White MA. Apoptosis - Interactions involving proteins with a function related to apoptosis Additional information on origin of constructs obtained from the author. michael.white@utsouthwestern.edu jyoti Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. fluorescence imaging fluorescence microscopy fluorescence imaging anti tag immunost anti tag immunostaining anti tag immunost Bodemann BO., Orvedahl A., Cheng T., Ram RR., Ou YH., Formstecher E., Maiti M., Hazelett CC., Wauson EM., Balakireva M., Camonis JH., Yeaman C., Levine B., White MA. Apoptosis - Interactions involving proteins with a function related to apoptosis Tagged molecules were visualised using tag fluorescence. Additional information on origin of constructs obtained from the author. michael.white@utsouthwestern.edu Accepted 2011-APR-19 by JYOTI Cell (0092-8674) 2011 jyoti Only protein-protein interactions imex curation atg5_human Autophagy protein 5 APG5-like Apoptosis-specific protein ATG5 APG5L ASP protein protein human Homo sapiens Human MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDISEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPSKDAIEAHFMSCMKEADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRKLMEYPAEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLKEVCPSAIDPEDGEKKNQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIIPQPTD DIP protein Q9H1Y0 original sequence version: 63 DIP protein Q9H1Y0 original sequence version: 56 DIP protein Q9H1Y0-1 original sequence version: 105 DIP protein Q9H1Y0 original sequence version: 105 C33A1E0B3C1DBE5C exoc8_human Exocyst complex component 8 Exocyst complex 84 kDa subunit EXOC8 protein protein human Homo sapiens Human MAMAMSDSGASRLRRQLESGGFEARLYVKQLSQQSDGDRDLQEHRQRIQALAEETAQNLKRNVYQNYRQFIETAREISYLESEMYQLSHLLTEQKSSLESIPLTLLPAAAAAGAAAASGGEEGVGGAGGRDHLRGQAGFFSTPGGASRDGSGPGEEGKQRTLTTLLEKVEGCRHLLETPGQYLVYNGDLVEYDADHMAQLQRVHGFLMNDCLLVATWLPQRRGMYRYNALYSLDGLAVVNVKDNPPMKDMFKLLMFPESRIFQAENAKIKREWLEVLEDTKRALSEKRRREQEEAAAPRGPPQVTSKATNPFEDDEEEEPAVPEVEEEKVDLSMEWIQELPEDLDVCIAQRDFEGAVDLLDKLNHYLEDKPSPPPVKELRAKVEERVRQLTEVLVFELSPDRSLRGGPKATRRAVSQLIRLGQCTKACELFLRNRAAAVHTAIRQLRIEGATLLYIHKLCHVFFTSLLETAREFEIDFAGTDSGCYSAFVVWARSAMGMFVDAFSKQVFDSKESLSTAAECVKVAKEHCQQLGDIGLDLTFIIHALLVKDIQGALHSYKEIIIEATKHRNSEEMWRRMNLMTPEALGKLKEEMKSCGVSNFEQYTGDDCWVNLSYTVVAFTKQTMGFLEEALKLYFPELHMVLLESLVEIILVAVQHVDYSLRCEQDPEKKAFIRQNASFLYETVLPVVEKRFEEGVGKPAKQLQDLRNASRLIRVNPESTTSVV F4E5E344EF24FDCA bakor_mouse Beclin 1-associated autophagy-related key regulator Atg14 D14Ertd436e Kiaa0831 Autophagy-related protein 14-like protein Atg14L protein protein mouse Mus musculus Mouse MASPSGKGSWTPEAPGFGPRALARDLVDSVDDAEGLYVAVERCPLCNTTRRRLTCAKCVQSGDFVYFDGRDRERFIDKKERLSQLKNKQEEFQKEVLKAMEGKRLTDQLRWKIMSCKMRIEQLKQTICKGNEEMKKNSEGLLKNKEKNQKLYSRAQRHQEKKEKIQRHNRKLGDLVEKKTIDLKSHYERLARLRRSHILELTSIIFPIDEVKTSGRDPADVSSETDSAMTSSMVSKLAEARRTTYLSGRWVCDDHNGDTSISITGPWISLPNNGDYSAYYNWVEEKKTTQGPDMEHNNPAYTISAALGYATQLVNIVSHILDINLPKKLCNSEFCGENLSKQKLTRAVRKLNANILYLCSSQHVNLDQLQPLHTLRNLMHLVSPRSEHLGRSGPFEVRADLEESMEFVDPGVAGESDASGDERVSDEETDLGTDWENLPSPRFCDIPSQPVEVSQSQSTQVSPPIASSSAGGMISSAAASVTSWFKAYTGHR DIP protein Q8CDJ3 original sequence version: 76 A221B3C03C8E2F9A atg12_human Ubiquitin-like protein ATG12 Autophagy-related protein 12 ATG12 APG12 APG12L protein protein human Homo sapiens Human MAEEPQSVLQLPTSIAAGGEGLTDVSPETTTPEPPSSAAVSPGTEEPAGDTKKKIDILLKAVGDTPIMKTKKWAVERTRTIQGLIDFIKKFLKLVASEQLFIYVNQSFAPSPDQEVGTLYECFGSDGKLVLHYCKSQAWG DIP protein O94817 original sequence version: 63 DIP protein O94817-1 original sequence version: 102 109258521C2D194F exoc2_human Exocyst complex component 2 EXOC2 SEC5 SEC5L1 Exocyst complex component Sec5 protein protein human Homo sapiens Human MSRSRQPPLVTGISPNEGIPWTKVTIRGENLGTGPTDLIGLTICGHNCLLTAEWMSASKIVCRVGQAKNDKGDIIVTTKSGGRGTSTVSFKLLKPEKIGILDQSAVWVDEMNYYDMRTDRNKGIPPLSLRPANPLGIEIEKSKFSQKDLEMLFHGMSADFTSENFSAAWYLIENHSNTSFEQLKMAVTNLKRQANKKSEGSLAYVKGGLSTFFEAQDALSAIHQKLEADGTEKVEGSMTQKLENVLNRASNTADTLFQEVLGRKDKADSTRNALNVLQRFKFLFNLPLNIERNIQKGDYDVVINDYEKAKSLFGKTEVQVFKKYYAEVETRIEALRELLLDKLLETPSTLHDQKRYIRYLSDLHASGDPAWQCIGAQHKWILQLMHSCKEGYVKDLKGNPGLHSPMLDLDNDTRPSVLGHLSQTASLKRGSSFQSGRDDTWRYKTPHRVAFVEKLTKLVLSQLPNFWKLWISYVNGSLFSETAEKSGQIERSKNVRQRQNDFKKMIQEVMHSLVKLTRGALLPLSIRDGEAKQYGGWEVKCELSGQWLAHAIQTVRLTHESLTALEIPNDLLQTIQDLILDLRVRCVMATLQHTAEEIKRLAEKEDWIVDNEGLTSLPCQFEQCIVCSLQSLKGVLECKPGEASVFQQPKTQEEVCQLSINIMQVFIYCLEQLSTKPDADIDTTHLSVDVSSPDLFGSIHEDFSLTSEQRLLIVLSNCCYLERHTFLNIAEHFEKHNFQGIEKITQVSMASLKELDQRLFENYIELKADPIVGSLEPGIYAGYFDWKDCLPPTGVRNYLKEALVNIIAVHAEVFTISKELVPRVLSKVIEAVSEELSRLMQCVSSFSKNGALQARLEICALRDTVAVYLTPESKSSFKQALEALPQLSSGADKKLLEELLNKFKSSMHLQLTCFQAASSTMMKT DIP protein Q96KP1 original sequence version: 80 2234E463DE8B076F rubic_mouse Run domain Beclin-1-interacting and cysteine-rich domain-containing protein Rubcn Kiaa0226 protein protein mouse Mus musculus Mouse MRPEGAGMDLGGGDGERLLEKSRREHWQLLGNLKTTVEGLVSANCPNVWSKYGGLERLCRDMQNILYHGLIHDQVCCRQADYWQFVKDIRWLSPHSALHVEKFISLHESDQSDTDSVSERAVAELWLQHSLQCHCLSAQLRPLLGDRQYIRKFYTETAFLLSDAHVTAMLQCLEAVEQNNPRLLAQIDASMFARKQESPLLVTKSQSLTALPGSTYTPPASYAQHSYFGSSSSLQSMPQSSHSSERRSTSFSLSGPSWQPQEDRECLSPAETQTTPAPLPSDSTLAQDSPLTAQEMSDSTLTSPLEASWVSSQNDSPSDVSEGPEYLAIGNPAPHGRTASCESHSSNGESSSSHLFSSSSSQKLESAASSLGDQEEGRQSQAGSVLRRSSFSEGQTAPVASGTKKSHIRSHSDTNIASRGAAGGPRNITIIVEDPIAEGGQYLCSGEGMFRRPSEGQSLISYLSEQDFGSCADLEKENAHFSISESLIAAIELMKCNMMSQCLEEEEVEEEDSDREIQELKQKIRLRRQQIRTKNLLPAYRETENGSFRVTSSSSQFSSRDSTQLSESGSAEDADDLEIQDADIRRSAVSNGKSSFSQNLSHCFLHSTSAEAVAMGLLKQFEGMQLPAASELEWLVPEHDAPQKLLPIPDSLPISPDDGQHADIYKLRIRVRGNLEWAPPRPQIIFNVHPAPTRKIAVAKQNYRCAGCGIRTDPDYIKRLRYCEYLGKYFCQCCHENAQMVVPSRILRKWDFSKYYVSNFSKDLLLKIWNDPLFNVQDINSALYRKVKLLNQVRLLRVQLYHMKNMFKTCRLAKELLDSFDVVPGHLTEDLHLYSLSDLTATKKGELGPRLAELTRAGAAHVERCMLCQAKGFICEFCQNEEDVIFPFELHKCRTCEECKACYHKTCFKSGRCPRCERLQARRELLAKQSLESYLSDYEEEPTEALALEATVLETT E5D8CD691C0D5184 atg5_mouse Autophagy protein 5 Atg5 Apg5l APG5-like protein protein mouse Mus musculus Mouse MTDDKDVLRDVWFGRIPTCFTLYQDEITEREAEPYYLLLPRVSYLTLVTDKVKKHFQKVMRQEDVSEIWFEYEGTPLKWHYPIGLLFDLLASSSALPWNITVHFKSFPEKDLLHCPSKDAVEAHFMSCMKEADALKHKSQVINEMQKKDHKQLWMGLQNDRFDQFWAINRKLMEYPPEENGFRYIPFRIYQTTTERPFIQKLFRPVAADGQLHTLGDLLREVCPSAVAPEDGEKRSQVMIHGIEPMLETPLQWLSEHLSYPDNFLHISIVPQPTD DIP protein Q99J83 original sequence version: 54 F3FCE652D627B694 exoc4_human Exocyst complex component 4 EXOC4 KIAA1699 SEC8 SEC8L1 Exocyst complex component Sec8 protein protein human Homo sapiens Human MAAEAAGGKYRSTVSKSKDPSGLLISVIRTLSTSDDVEDRENEKGRLEEAYEKCDRDLDELIVQHYTELTTAIRTYQSITERITNSRNKIKQVKENLLSCKMLLHCKRDELRKLWIEGIEHKHVLNLLDEIENIKQVPQKLEQCMASKHYLSATDMLVSAVESLEGPLLQVEGLSDLRLELHSKKMNLHLVLIDELHRHLYIKSTSRVVQRNKEKGKISSLVKDASVPLIDVTNLPTPRKFLDTSHYSTAGSSSVREINLQDIKEDLELDPEENSTLFMGILIKGLAKLKKIPETVKAIIERLEQELKQIVKRSTTQVADSGYQRGENVTVENQPRLLLELLELLFDKFNAVAAAHSVVLGYLQDTVVTPLTQQEDIKLYDMADVWVKIQDVLQMLLTEYLDMKNTRTASEPSAQLSYASTGREFAAFFAKKKPQRPKNSLFKFESSSHAISMSAYLREQRRELYSRSGELQGGPDDNLIEGGGTKFVCKPGARNITVIFHPLLRFIQEIEHALGLGPAKQCPLREFLTVYIKNIFLNQVLAEINKEIEGVTKTSDPLKILANADTMKVLGVQRPLLQSTIIVEKTVQDLLNLMHDLSAYSDQFLNMVCVKLQEYKDTCTAAYRGIVQSEEKLVISASWAKDDDISRLLKSLPNWMNMAQPKQLRPKREEEEDFIRAAFGKESEVLIGNLGDKLIPPQDILRDVSDLKALANMHESLEWLASRTKSAFSNLSTSQMLSPAQDSHTNTDLPPVSEQIMQTLSELAKSFQDMADRCLLVLHLEVRVHCFHYLIPLAKEGNYAIVANVESMDYDPLVVKLNKDISAIEEAMSASLQQHKFQYIFEGLGHLISCILINGAQYFRRISESGIKKMCRNIFVLQQNLTNITMSREADLDFARQYYEMLYNTADELLNLVVDQGVKYTELEYIHALTLLHRSQTGVGELTTQNTRLQRLKEIICEQAAIKQATKDKKITTV DIP protein Q96A65 original sequence version: 76 DIP protein Q96A65 original sequence version: 88 DIP protein Q96A65 original sequence version: 135 122CD929C2635786 exoc7_human Exocyst complex component 7 EXOC7 EXO70 KIAA1067 Exocyst complex component Exo70 protein protein human Homo sapiens Human MIPPQEASARRREIEDKLKQEEETLSFIRDSLEKSDQLTKNMVSILSSFESRLMKLENSIIPVHKQTENLQRLQENVEKTLSCLDHVISYYHVASDTEKIIREGPTGRLEEYLGSMAKIQKAVEYFQDNSPDSPELNKVKLLFERGKEALESEFRSLMTRHSKVVSPVLILDLISGDDDLEAQEDVTLEHLPESVLQDVIRISRWLVEYGRNQDFMNVYYQIRSSQLDRSIKGLKEHFHKSSSSSGVPYSPAIPNKRKDTPTKKPVKRPGTIRKAQNLLKQYSQHGLDGKKGGSNLIPLEGLLPCTPRGGLPGPWINAACVCAADISPGHEHDFRVKHLSEALNDKHGPLAGRDDMLDVETDAYIHCVSAFVKLAQSEYQLLADIIPEHHQKKTFDSLIQDALDGLMLEGENIVSAARKAIVRHDFSTVLTVFPILRHLKQTKPEFDQVLQGTAASTKNKLPGLITSMETIGAKALEDFADNIKNDPDKEYNMPKDGTVHELTSNAILFLQQLLDFQETAGAMLASQETSSSATSYSSEFSKRLLSTYICKVLGNLQLNLLSKSKVYEDPALSAIFLHNNYNYILKSLEKSELIQLVAVTQKTAERSYREHIEQQIQTYQRSWLKVTDYIAEKNLPVFQPGVKLRDKERQIIKERFKGFNDGLEELCKIQKAWAIPDTEQRDRIRQAQKTIVKETYGAFLQKFGSVPFTKNPEKYIKYGVEQVGDMIDRLFDTSA DIP protein Q9UPT5 original sequence version: 142 6B929762542854B6 gbrl1_rat Gamma-aminobutyric acid receptor-associated protein-like 1 Gabarapl1 Gec1 GABA(A) receptor-associated protein-like 1 Glandular epithelial cell protein 1 protein protein rat Rattus norvegicus (Rat) Rat MKFQYKEDHPFEYRKKEGEKIRKKYPDRVPVIVEKAPKARVPDLDKRKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNTIPPTSATMGQLYEDNHEEDYFLYVAYSDESVYGK 7F56E345255F564B ralb_human Ras-related protein Ral-B RALB protein protein human Homo sapiens Human MAANKSKGQSSLALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLLVFSITEHESFTATAEFREQILRVKAEEDKIPLLVVGNKSDLEERRQVPVEEARSKAEEWGVQYVETSAKTRANVDKVFFDLMREIRTKKMSENKDKNGKKSSKNKKSFKERCCLL E0AC95130FB6452C ralb_macfa Ras-related protein Ral-B RALB QtsA-18830 protein protein macfa Macaca fascicularis (Crab-eating macaque) Cynomolgus monkey MAANKSKGQSSLALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLLVFSITEHESFTATAEFREQILRVKAEEDKIPLLVVGNKSDLEERRQVPVEEARSKAEEWGVQYVETSAKTRANVDKVFFDLMREIRTKKMSENKDKNGKKSGKNKKSFKERCCLL E0AC95130FA7012C exoc2_rat Exocyst complex component 2 Exoc2 Sec5 Sec5l1 Exocyst complex component Sec5 protein protein rat Rattus norvegicus (Rat) Rat MSRSRQPPLVTGISPNEGIPWTKVTIRGENLGTGPTDLIGLTICGHNCLLTAEWMSASKIVCRVGQAKNDKGDIIVTTKSGGRGTSTVSFKLLKPEKIGILDQSAVWVDEMNYYDMRTDRNKGIPPLSLRPANPLGIEIEKCKLPQKNLEVLFHGMSADFTSENFSAAWYLIENHSNTSFEQLKMAVTNLKRQANKKSEGSLAYVKGGLSTFFEAQDALSAIHQKLEADGTEKVEGSMTQKLENVLNRASNTADTLFQEVLGRKDKADSTRNALNVLQRFKFLFNLPLNIKRNIQKGDYDVVINDYEKAKSLFGKTEVQVFKKYYAEVEAGIEDLRELLLKKLLETPSTLHDQKRYIRYLSDLHAPGDPAWQCIGAQHKWTLKLMQDCKEGHMKSLKGNPGPHSPMLDLDNDARPSVLGHLSQTASLKRGSSFQSGRDDTWRYKTPHRVAFVEKLTKLVLSQLPNFWKLWISYVNGSLFSETAEKSGQIERSKNVRQRQNDFKKMIQEVMHSLVKLIRGALLPFSLREGDGRQYGGWEVQAELSGQWLAHVIQTIRLTYESLTALEIPNDMLQIIQDLILDLRIHCIMVTLQHTAEEIKRLAEKEDWIVDNEGLTSLPCQFEQSIVHSLQSLKGVVDCKPGEASVFQQPKTQEEVCQLCISIMQVFIYCLEQLSTKPDADIDTTHLSVDVSSPDLFGSIHEDFGLTSEQRLLIVLSNCCYLERHTFLNIAEHFEKHNFQGIEKITQVSMASLKELDQRLFENYIELKADPIVGSLETGIYAGYFDWKDCLPPAGVRNYLKEALVNIIAVHAEVFTISKELVPRVLARVIEAVSEELSRLMQCVSSFSRNGALQARLEICALRDTVAIYLTPESRSSFKQALEALPQLASGADKKLLEELLNKFKSSMHLQLTCFQAASPTVMKT 1903C0593B113373 rala_human Ras-related protein Ral-A RAL RALA protein protein human Homo sapiens Human MAANKPKGQNSLALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLCVFSITEMESFAATADFREQILRVKEDENVPFLLVGNKSDLEDKRQVSVEEAKNRAEQWNVNYVETSAKTRANVDKVFFDLMREIRARKMEDSKEKNGKKKRKSLAKRIRERCCIL 6974341EA18C1975 becn1_human Beclin-1 Coiled-coil myosin-like BCL2-interacting protein Protein GT197 BECN1 GT197 protein protein human Homo sapiens Human MEGSKTSNNSTMQVSFVCQRCSQPLKLDTSFKILDRVTIQELTAPLLTTAQAKPGETQEEETNSGEEPFIETPRQDGVSRRFIPPARMMSTESANSFTLIGEASDGGTMENLSRRLKVTGDLFDIMSGQTDVDHPLCEECTDTLLDQLDTQLNVTENECQNYKRCLEILEQMNEDDSEQLQMELKELALEEERLIQELEDVEKNRKIVAENLEKVQAEAERLDQEEAQYQREYSEFKRQQLELDDELKSVENQMRYAQTQLDKLKKTNVFNATFHIWHSGQFGTINNFRLGRLPSVPVEWNEINAAWGQTVLLLHALANKMGLKFQRYRLVPYGNHSYLESLTDKSKELPLYCSGGLRFFWDNKFDHAMVAFLDCVQQFKEEVEKGETRFCLPYRMDVEKGKIEDTGGSGGSYSIKTQFNSEEQWTKALKFMLTNLKWGLAWVSSQFYNK DIP protein Q14457 original sequence version: 95 DIP protein Q14457 original sequence version: 156 DIP protein Q14457 original sequence version: 122 DIP protein Q14457 original sequence version: 120 DIP protein Q14457 original sequence version: 133 DIP protein Q14457 original sequence version: 141 DIP protein Q14457 original sequence version: 106 ABF0C2DD7087473C exoc1_human Exocyst complex component 1 Exocyst complex component Sec3 EXOC1 SEC3 SEC3L1 BM-012 protein protein human Homo sapiens Human MTAIKHALQRDIFTPNDERLLSIVNVCKAGKKKKNCFLCATVTTERPVQVKVVKVKKSDKGDFYKRQIAWALRDLAVVDAKDAIKENPEFDLHFEKIYKWVASSTAEKNAFISCIWKLNQRYLRKKIDFVNVSSQLLEESVPSGENQSVTGGDEEVVDEYQELNAREEQDIEIMMEGCEYAISNAEAFAEKLSRELQVLDGANIQSIMASEKQVNILMKLLDEALKEVDQIELKLSSYEEMLQSVKEQMDQISESNHLIHLSNTNNVKLLSEIEFLVNHMDLAKGHIKALQEGDLASSRGIEACTNAADALLQCMNVALRPGHDLLLAVKQQQQRFSDLRELFARRLASHLNNVFVQQGHDQSSTLAQHSVELTLPNHHPFHRDLLRYAKLMEWLKSTDYGKYEGLTKNYMDYLSRLYEREIKDFFEVAKIKMTGTTKESKKFATLPRKESAVKQETESLHGSSGKLTGSTSSLNKLSVQSSGNRRSQSSSLLDMGNMSASDLDVADRTKFDKIFEQVLSELEPLCLAEQDFISKFFKLQQHQSMPGTMAEAEDLDGGTLSRQHNCGTPLPVSSEKDMIRQMMIKIFRCIEPELNNLIALGDKIDSFNSLYMLVKMSHHVWTAQNVDPASFLSTTLGNVLVTVKRNFDKCISNQIRQMEEVKISKKSKVGILPFVAEFEEFAGLAESIFKNAERRGDLDKAYTKLIRGVFVNVEKVANESQKTPRDVVMMENFHHIFATLSRLKISCLEAEKKEAKQKYTDHLQSYVIYSLGQPLEKLNHFFEGVEARVAQGIREEEVSYQLAFNKQELRKVIKEYPGKEVKKGLDNLYKKVDKHLCEEENLLQVVWHSMQDEFIRQYKHFEGLIARCYPGSGVTMEFTIQDILDYCSSIAQSH DIP protein Q9NV70 original sequence version: 75 F63C88E3A7F611BD rubic-exoc1 3 12 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1A bakor-exoc1 3 9 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1B exoc8-rubic 3 8 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1C exoc2-rubic 3 19 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1D exoc8-bakor 3 9 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1B exoc2-bakor 3 19 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1F exoc4-atg12-1 1 10 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey association association Fig. 1H ATG5/ATG12 antibody has been raised against the conjugated heterodimer rather than the individual proteins. becn1-ralb 5 21 unspecified role unspecified role neutral component neutral component 17 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 3A Beclin1 and RalB colocalize in cells pre and post induction of autophagy atg5-ralb 5 17 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 3C In addition, we found marked colocalization of endogenous RalB with GFP-ATG5 after a 90 min incubation in starvation media (Figure 3C) gbrl1-ralb 5 17 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 3D By 4 hr, GFP-LC3 punctae had accumulated, many of which were RalB positive (Figure 3D). ralb-atg12 1 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 17 unspecified role unspecified role bait bait association association Fig. 3G ATG5/ATG12 antibody has been raised against the conjugated heterodimer rather than the individual proteins. Sendai virus infection exoc8-becn1-1 3 21 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 4A, Supp. Figure 2A Nutrient starvation exoc2-becn1-2 3 19 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 21 unspecified role unspecified role prey prey Q14457:p.Phe123Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A Wed May 02 16:47:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe123ala' Tue Jun 30 17:54:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe123Ala' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association Figs. 4B, 4C, 4F, Supp. Fig. 2A Nutrient starvation Complex disassembly induced by nutrient starvation was reversed by addition of non-essential amino acids. becn1-exoc8-1 1 21 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association Fig. 4G Importantly, we found that immunoprecipitation of endogenous Beclin1 from nutrient-deprived versus nutrient replete cells resulted in selective coprecipitation of endogenous Exo84 under starvation conditions Nutrient deprivation becn1-becn1 6 8 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag certain certain sequence position certain certain certain sequence position certain 21 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 4I exoc2-becn1-1 6 19 unspecified role unspecified role neutral component neutral component region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 21 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Figs. 4J exoc2-rala-2 4 19 unspecified role unspecified role bait bait rbd region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag certain certain sequence position certain certain certain sequence position certain undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 20 unspecified role unspecified role prey prey human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. 17 unspecified role unspecified role prey prey human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. association association Fig. 5A RalA and RalB are GTP-bound exoc4-atg5 3 14 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association exoc4-becn1 3 14 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 21 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 4D exoc8-becn1-2 2 8 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 21 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 14 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q4R379:p.Gly23Val mutation mutation certain certain sequence position certain certain certain sequence position certain G V Wed May 02 15:38:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly23val' Tue Jun 30 17:08:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly23Val' association association Fig. 5B exoc8-becn1-3 3 14 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 5B