IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. J. Biol. Chem. (0021-9258) 2011 imex curation Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr 21-Dec-2011: Contacted by JYOTI. Human Jurkat T cell cDNA Only protein-protein interactions human-697 Homo sapiens 697 Dexamethasone-resistant human Pre-B leukemia 697 cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr Accepted 2011-SEP-13 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Human Jurkat T cell cDNA J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation For Fig. 3 you say "I would specify the isoform for Tid1 as this expt does not tell you if Stat5b is interacting with one or both isoforms" - I am assuming you meant that you wouldn't specify the isoform. The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. J. Biol. Chem. (0021-9258) 2011 imex curation Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr 21-Dec-2011: Contacted by JYOTI. Human Jurkat T cell cDNA Only protein-protein interactions chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr Accepted 2011-SEP-13 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Human Jurkat T cell cDNA J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation Short label for EBI-4322290 was automatically created as dnaja3-dnaja3 - which is not correct, should I change this manually? The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. J. Biol. Chem. (0021-9258) 2011 imex curation Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr 21-Dec-2011: Contacted by JYOTI. Human Jurkat T cell cDNA Only protein-protein interactions human-jurkat Homo sapiens jurkat Human T cell leukemia anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr Accepted 2011-SEP-13 by ORCHARD Human Jurkat T cell cDNA J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. J. Biol. Chem. (0021-9258) 2011 imex curation Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr 21-Dec-2011: Contacted by JYOTI. Human Jurkat T cell cDNA Only protein-protein interactions mouse-ba_f3 Mouse pro B cells IL-3 dependent mouse-baf3 EBI-6922762 ba_f3 Mouse pro B cells IL-3 dependent BA/F3 baf3 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr Accepted 2011-SEP-13 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Human Jurkat T cell cDNA J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation For Fig. 3 you say "I would specify the isoform for Tid1 as this expt does not tell you if Stat5b is interacting with one or both isoforms" - I am assuming you meant that you wouldn't specify the isoform. The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. J. Biol. Chem. (0021-9258) 2011 imex curation Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr 21-Dec-2011: Contacted by JYOTI. Human Jurkat T cell cDNA Only protein-protein interactions ecolx Escherichia coli 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr Accepted 2011-SEP-13 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Human Jurkat T cell cDNA J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. The tumor suppressor hTid1 inhibits STAT5b activity via functional interaction. J. Biol. Chem. (0021-9258) 2011 imex curation Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr 21-Dec-2011: Contacted by JYOTI. Human Jurkat T cell cDNA Only protein-protein interactions ecolx Escherichia coli 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Dhennin-Duthille I., Nyga R., Yahiaoui S., Gouilleux-Gruart V., Regnier A., Lassoued K., Gouilleux F. Apoptosis - Interactions involving proteins with a function related to apoptosis fabrice.gouilleux@univ-tours.fr Accepted 2011-SEP-13 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Human Jurkat T cell cDNA J. Biol. Chem. (0021-9258) 2011 Only protein-protein interactions imex curation sta5b_human Signal transducer and activator of transcription 5B STAT5B protein protein human Homo sapiens Human MAVWIQAQQLQGEALHQMQALYGQHFPIEVRHYLSQWIESQAWDSVDLDNPQENIKATQLLEGLVQELQKKAEHQVGEDGFLLKIKLGHYATQLQNTYDRCPMELVRCIRHILYNEQRLVREANNGSSPAGSLADAMSQKHLQINQTFEELRLVTQDTENELKKLQQTQEYFIIQYQESLRIQAQFGPLAQLSPQERLSRETALQQKQVSLEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCEKLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQTKFAATVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNDYSGEILNNCCVMEYHQATGTLSAHFRNMSLKRIKRSDRRGAESVTEEKFTILFESQFSVGGNELVFQVKTLSLPVVVIVHGSQDNNATATVLWDNAFAEPGRVPFAVPDKVLWPQLCEALNMKFKAEVQSNRGLTKENLVFLAQKLFNNSSSHLEDYSGLSVSWSQFNRENLPGRNYTFWQWFDGVMEVLKKHLKPHWNDGAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSQERMFWNLMPFTTRDFSIRSLADRLGDLNYLIYVFPDRPKDEVYSKYYTPVPCESATAKAVDGYVKPQIKQVVPEFVNASADAGGGSATYMDQAPSPAVCPQAHYNMYPQNPDSVLDTDGDFDLEDTMDVARRVEELLGRPMDSQWIPHAQS AA2F1CAB20955ACA q96ey1-2 DnaJ homolog subfamily A member 3, mitochondrial DNAJA3 HCA57 TID1 DnaJ protein Tid-1 Hepatocellular carcinoma-associated antigen 57 Tumorous imaginal discs protein Tid56 homolog Tid-1(S) protein protein human Homo sapiens Human MAARCSTRWLLVVVGTPRLPAISGRGARPPREGVVGAWLSRKLSVPAFASSLTSCGPRALLTLRPGVSLTGTKHNPFICTASFHTSAPLAKEDYYQILGVPRNASQKEIKKAYYQLAKKYHPDTNKDDPKAKEKFSQLAEAYEVLSDEVKRKQYDAYGSAGFDPGASGSQHSYWKGGPTVDPEELFRKIFGEFSSSSFGDFQTVFDQPQEYFMELTFNQAAKGVNKEFTVNIMDTCERCNGKGNEPGTKVQHCHYCGGSGMETINTGPFVMRSTCRRCGGRGSIIISPCVVCRGAGQAKQKKRVMIPVPAGVEDGQTVRMPVGKREIFITFRVQKSPVFRRDGADIHSDLFISIAQALLGGTARAQGLYETINVTIPPGTQTDQKIRMGGKGIPRINSYGYGDHYIHIKIRVPKRLTSRQQSLILSYAEDETDVEGTVNGVTLTSSGKRSTGN E235B8DC1DB99FBC sta5b_mouse Signal transducer and activator of transcription 5B Stat5b protein protein mouse Mus musculus Mouse MAMWIQAQQLQGDALHQMQALYGQHFPIEVRHYLSQWIESQAWDSIDLDNPQENIKATQLLEGLVQELQKKAEHQVGEDGFLLKIKLGHYATQLQSTYDRCPMELVRCIRHILYNEQRLVREANNGSSPAGSLADAMSQKHLQINQTFEELRLITQDTENELKKLQQTQEYFIIQYQESLRIQAQFAQLGQLNPQERMSRETALQQKQVSLETWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQQLAGNGGPPEGSLDVLQSWCEKLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNATITDIISALVTSTFIIEKQPPQVLKTQTKFAATVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRNDYSGEILNNCCVMEYHQATGTLSAHFRNMSLKRIKRSDRRGAESVTEEKFTILFDSQFSVGGNELVFQVKTLSLPVVVIVHGSQDNNATATVLWDNAFAEPGRVPFAVPDKVLWPQLCEALNMKFKAEVQSNRGLTKENLVFLAQKLFNISSNHLEDYNSMSVSWSQFNRENLPGRNYTFWQWFDGVMEVLKKHLKPHWNDGAILGFVNKQQAHDLLINKPDGTFLLRFSDSEIGGITIAWKFDSQERMFWNLMPFTTRDFSIRSLADRLGDLNYLIYVFPDRPKDEVYSKYYTPVPCEPATAKAADGYVKPQIKQVVPEFANASTDAGSGATYMDQAPSPVVCPQAHYNMYPPNPDSVLDTDGDFDLEDTMDVARRVEELLGRPMDSQWIPHAQS A8FE76405E41B2EF q96ey1-1 DnaJ homolog subfamily A member 3, mitochondrial Tid-1(L) DNAJA3 HCA57 TID1 DnaJ protein Tid-1 Hepatocellular carcinoma-associated antigen 57 Tumorous imaginal discs protein Tid56 homolog protein protein human Homo sapiens Human MAARCSTRWLLVVVGTPRLPAISGRGARPPREGVVGAWLSRKLSVPAFASSLTSCGPRALLTLRPGVSLTGTKHNPFICTASFHTSAPLAKEDYYQILGVPRNASQKEIKKAYYQLAKKYHPDTNKDDPKAKEKFSQLAEAYEVLSDEVKRKQYDAYGSAGFDPGASGSQHSYWKGGPTVDPEELFRKIFGEFSSSSFGDFQTVFDQPQEYFMELTFNQAAKGVNKEFTVNIMDTCERCNGKGNEPGTKVQHCHYCGGSGMETINTGPFVMRSTCRRCGGRGSIIISPCVVCRGAGQAKQKKRVMIPVPAGVEDGQTVRMPVGKREIFITFRVQKSPVFRRDGADIHSDLFISIAQALLGGTARAQGLYETINVTIPPGTQTDQKIRMGGKGIPRINSYGYGDHYIHIKIRVPKRLTSRQQSLILSYAEDETDVEGTVNGVTLTSSGGSTMDSSAGSKARREAGEDEEGFLSKLKKMFTS 5A57B9020992CF59 stat1_human Signal transducer and activator of transcription 1-alpha/beta STAT1 Transcription factor ISGF-3 components p91/p84 protein protein human Homo sapiens Human MSQWYELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQHNIRKSKRNLQDNFQEDPIQMSMIIYSCLKEERKILENAQRFNQAQSGNIQSTVMLDKQKELDSKVRNVKDKVMCIEHEIKSLEDLQDEYDFKCKTLQNREHETNGVAKSDQKQEQLLLKKMYLMLDNKRKEVVHKIIELLNVTELTQNALINDELVEWKRRQQSACIGGPPNACLDQLQNWFTIVAESLQQVRQQLKKLEELEQKYTYEHDPITKNKQVLWDRTFSLFQQLIQSSFVVERQPCMPTHPQRPLVLKTGVQFTVKLRLLVKLQELNYNLKVKVLFDKDVNERNTVKGFRKFNILGTHTKVMNMEESTNGSLAAEFRHLQLKEQKNAGTRTNEGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVAEPRNLSFFLTPPCARWAQLSEVLSWQFSSVTKRGLNVDQLNMLGEKLLGPNASPDGLIPWTRFCKENINDKNFPFWLWIESILELIKKHLLPLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHAVEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDGPKGTGYIKTELISVSEVHPSRLQTTDNLLPMSPEEFDEVSRIVGSVEFDSMMNTV 054A813522364BA6 dnja3_human DnaJ homolog subfamily A member 3, mitochondrial DNAJA3 TID1 HCA57 Tumorous imaginal discs protein Tid56 homolog DnaJ protein Tid-1 Hepatocellular carcinoma-associated antigen 57 protein protein human Homo sapiens Human MAARCSTRWLLVVVGTPRLPAISGRGARPPREGVVGAWLSRKLSVPAFASSLTSCGPRALLTLRPGVSLTGTKHNPFICTASFHTSAPLAKEDYYQILGVPRNASQKEIKKAYYQLAKKYHPDTNKDDPKAKEKFSQLAEAYEVLSDEVKRKQYDAYGSAGFDPGASGSQHSYWKGGPTVDPEELFRKIFGEFSSSSFGDFQTVFDQPQEYFMELTFNQAAKGVNKEFTVNIMDTCERCNGKGNEPGTKVQHCHYCGGSGMETINTGPFVMRSTCRRCGGRGSIIISPCVVCRGAGQAKQKKRVMIPVPAGVEDGQTVRMPVGKREIFITFRVQKSPVFRRDGADIHSDLFISIAQALLGGTARAQGLYETINVTIPPGTQTDQKIRMGGKGIPRINSYGYGDHYIHIKIRVPKRLTSRQQSLILSYAEDETDVEGTVNGVTLTSSGGSTMDSSAGSKARREAGEDEEGFLSKLKKMFTS DIP protein Q96EY1 original sequence version: 121 5A57B9020992CF59 stat1_mouse Signal transducer and activator of transcription 1 Stat1 protein protein mouse Mus musculus Mouse MSQWFELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAAYDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQHNIRKSKRNLQDNFQEDPVQMSMIIYNCLKEERKILENAQRFNQAQEGNIQNTVMLDKQKELDSKVRNVKDQVMCIEQEIKTLEELQDEYDFKCKTSQNREGEANGVAKSDQKQEQLLLHKMFLMLDNKRKEIIHKIRELLNSIELTQNTLINDELVEWKRRQQSACIGGPPNACLDQLQTWFTIVAETLQQIRQQLKKLEELEQKFTYEPDPITKNKQVLSDRTFLLFQQLIQSSFVVERQPCMPTHPQRPLVLKTGVQFTVKSRLLVKLQESNLLTKVKCHFDKDVNEKNTVKGFRKFNILGTHTKVMNMEESTNGSLAAELRHLQLKEQKNAGNRTNEGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVTEPRNLSFFLNPPCAWWSQLSEVLSWQFSSVTKRGLNADQLSMLGEKLLGPNAGPDGLIPWTRFCKENINDKNFSFWPWIDTILELIKNDLLCLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHAVEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDDPKRTGYIKTELISVSEVHPSRLQTTDNLLPMSPEEFDEMSRIVGPEFDSMMSTV DIP protein P42225 original sequence version: 131 DIP protein P42225 original sequence version: 71 249D919952BE65F1 stat5b-dnaja3 6 9 unspecified role unspecified role bait bait region activation domain activation domain tag activation domain certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role prey prey physical association physical association stat5b-dnaja3-1 5 12 unspecified role unspecified role prey prey region binding region binding-associated region binding region range range range range 9 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 22 20 physical association physical association Fig. 1B dnaja3-dnaja3 2 9 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged anti tag western anti tag western blot anti tag western undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait physical association physical association Fig. 2A dnaja3-stat5b-3 3 12 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Fig. 2B dnaja3-stat5b-5 1 7 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association Fig. 2B, 3B dnaja3-stat5b-6 4 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait physical association physical association Fig. 2C dnaja3-stat5b-7 4 9 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Fig. 2C dnaja3-stat5b-8 1 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Fig. 2C dnaja3-stat5b-9 1 10 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Fig. 2C stat5b-dnaja3-2 2 10 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait physical association physical association Fig. 5B dnaja3-stat1-2 1 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait physical association physical association Figs. 2D, 3C dnaja3-stat5b-11 4 12 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association Fig. 3A dnaja3-stat1-3 4 12 unspecified role unspecified role bait bait 13 unspecified role unspecified role prey prey physical association physical association Figs. 2D, 3C