IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ TGF-beta signaling specifies axons during brain development. TGF-beta signaling specifies axons during brain development. Cell (0092-8674) 2010 imex curation Yi JJ., Barnes AP., Hand R., Polleux F., Ehlers MD. Apoptosis - Interactions involving proteins with a function related to apoptosis Nothing found in the Synapse Dataset. ehlers@neuro.duke.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions mouse-neocortex Mus musculus Neocortex neocortex NEOCORTEX The neocortex is the top layer of the cerebral hemispheres, 2-4 mm thick, and made up of six layers, labelled I to VI (with VI being the innermost and I being the outermost). It is involved in higher functions such as sensory perception, generation of motor commands, spatial reasoning, conscious thought, and in humans, language. confocal microscopy confocal microscopy immunostaining immunostaining Yi JJ., Barnes AP., Hand R., Polleux F., Ehlers MD. Apoptosis - Interactions involving proteins with a function related to apoptosis Nothing found in the Synapse Dataset. ehlers@neuro.duke.edu Accepted 2010-OCT-3 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation TGF-beta signaling specifies axons during brain development. TGF-beta signaling specifies axons during brain development. Cell (0092-8674) 2010 imex curation Yi JJ., Barnes AP., Hand R., Polleux F., Ehlers MD. Apoptosis - Interactions involving proteins with a function related to apoptosis Nothing found in the Synapse Dataset. ehlers@neuro.duke.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions mouse-neocortex Mus musculus Neocortex neocortex NEOCORTEX The neocortex is the top layer of the cerebral hemispheres, 2-4 mm thick, and made up of six layers, labelled I to VI (with VI being the innermost and I being the outermost). It is involved in higher functions such as sensory perception, generation of motor commands, spatial reasoning, conscious thought, and in humans, language. confocal microscopy confocal microscopy immunostaining immunostaining Yi JJ., Barnes AP., Hand R., Polleux F., Ehlers MD. Apoptosis - Interactions involving proteins with a function related to apoptosis Nothing found in the Synapse Dataset. ehlers@neuro.duke.edu Accepted 2010-OCT-3 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation TGF-beta signaling specifies axons during brain development. TGF-beta signaling specifies axons during brain development. Cell (0092-8674) 2010 imex curation Yi JJ., Barnes AP., Hand R., Polleux F., Ehlers MD. Apoptosis - Interactions involving proteins with a function related to apoptosis Nothing found in the Synapse Dataset. ehlers@neuro.duke.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions rat-embryonic_forebrain Rattus norvegicus embryonic forebrain embryonic_forebrain Embryo forebrain anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yi JJ., Barnes AP., Hand R., Polleux F., Ehlers MD. Apoptosis - Interactions involving proteins with a function related to apoptosis Nothing found in the Synapse Dataset. ehlers@neuro.duke.edu Accepted 2010-OCT-3 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation TGF-beta signaling specifies axons during brain development. TGF-beta signaling specifies axons during brain development. Cell (0092-8674) 2010 imex curation Yi JJ., Barnes AP., Hand R., Polleux F., Ehlers MD. Apoptosis - Interactions involving proteins with a function related to apoptosis Nothing found in the Synapse Dataset. ehlers@neuro.duke.edu 21-Dec-2010: Contacted by JYOTI. jyoti Only protein-protein interactions rat-hippocampus Rattus norvegicus hippocampal neurons hippocampus Hippocampus [Brain hippocampus] confocal microscopy confocal microscopy immunostaining immunostaining Yi JJ., Barnes AP., Hand R., Polleux F., Ehlers MD. Apoptosis - Interactions involving proteins with a function related to apoptosis Nothing found in the Synapse Dataset. ehlers@neuro.duke.edu Accepted 2010-OCT-3 by JYOTI. 21-Dec-2010: Contacted by JYOTI. Cell (0092-8674) 2010 jyoti Only protein-protein interactions imex curation tgfr1_mouse TGF-beta receptor type-1 Tgfbr1 Transforming growth factor-beta receptor type I ESK2 protein protein mouse Mus musculus Mouse MEAAAAAPRRPQLLIVLVAAATLLPGAKALQCFCHLCTKDNFTCETDGLCFVSVTETTDKVIHNSMCIAEIDLIPRDRPFVCAPSSKTGAVTTTYCCNQDHCNKIELPTTGPFSEKQSAGLGPVELAAVIAGPVCFVCIALMLMVYICHNRTVIHHRVPNEEDPSLDRPFISEGTTLKDLIYDMTTSGSGSGLPLLVQRTIARTIVLQESIGKGRFGEVWRGKWRGEEVAVKIFSSREERSWFREAEIYQTVMLRHENILGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLNRYTVTVEGMIKLALSTASGLAHLHMEIVGTQGKPAIAHRDLKSKNILVKKNGTCCIADLGLAVRHDSATDTIDIAPNHRVGTKRYMAPEVLDDSINMKHFESFKRADIYAMGLVFWEIARRCSIGGIHEDYQLPYYDLVPSDPSVEEMRKVVCEQKLRPNIPNRWQSCEALRVMAKIMRECWYANGAARLTALRIKKTLSQLSQQEGIKM DIP protein Q64729 original sequence version: 120 DIP protein Q64729 original sequence version: 131 DIP protein Q64729 original sequence version: 157 BB8BB6D2261793AF par6a_rat Partitioning defective 6 homolog alpha Pard6a Par-6a Par6a protein protein rat Rattus norvegicus (Rat) Rat MARPQRTPARSPDSIVEVKSKFDAEFRRFALPRTSVRGFQEFSRLLCVVHQIPGLDVLLGYTDAHGDLLPLTNDDSLHRALASGPPPLRLLVQKRAEGDSSGLAFASNSLQRRKKGLLLRPVAPLRTRPPLLISLPQDFRQVSSVIDVDLLPETHRRVRLHKHGSDRPLGFYIRDGMSVRVAPQGLERVPGIFISRLVRGGLAESTGLLAVSDEILEVNGIEVAGKTLDQVTDMMVANSHNLIVTVKPANQRNNVVRGASGRLTGPSSVGPGPTDPDSDDDNSDPVIENRHPPCSNGLSQGPLCWDLQPGCLHPSAGSSLPSLDSREQANSGWGNGMRGDVSGFSL not sure about protein subtype, an alpha form was given by me DIP protein Q6B4M5 original sequence version: 40 E363D7F8960DEF7F tgfr1_rat TGF-beta receptor type-1 Tgfbr1 Transforming growth factor-beta receptor type I TGF-beta type I receptor Serine/threonine-protein kinase receptor R4 protein protein rat Rattus norvegicus (Rat) Rat MEAASAALRRCLLLIVLVAAATLLPGAKALQCFCHLCTKDNFTCETDGLCFVSVTETTDKVIHNSMCIAEIDLIPRDRPFVCAPSSKTGAVTYCCNQDHCNKIELPTTGPFSEKQSAGLGPVELAAVIAGPVCFVCIALMLMVYICHNRTVIHHRVPNEEDPSLDRPFISEGTTLKDLIYDMTTSGSGSGLPLLVQRTIARTIVLQESIGKGRFGEVWRGKWRGEEVAVKIFSSREERSWFREAEIYQTVMLRHENILGFIAADNKDNGTWTQLWLVSDYHEHGSLFDYLNRYTVTVEGMIKLALSTASGLAHLHMEIVGTQGKPAIAHRDLKSKNILVKKNGTCCIADLGLAVRHDSATDTIDIAPNHRVGTKRYMAPEVLDDSINMKHFESFKRADIYAMGLVFWEIARRCSIGGIHEDYQLPYYDLVPSDPSVEEMRKVVCEQKLRPNIPNRWQSCEALRVMAKIMRECWYANGAARLTALRIKKTLSQLSQQEGIKM EAD7F8D37E456102 tgfr2_mouse TGF-beta receptor type-2 Tgfbr2 Transforming growth factor-beta receptor type II TGF-beta type II receptor protein protein mouse Mus musculus Mouse MGRGLLRGLWPLHIVLWTRIASTIPPHVPKSDVEMEAQKDASIHLSCNRTIHPLKHFNSDVMASDNGGAVKLPQLCKFCDVRLSTCDNQKSCMSNCSITAICEKPHEVCVAVWRKNDKNITLETVCHDPKLTYHGFTLEDAASPKCVMKEKKRAGETFFMCACNMEECNDYIIFSEEYTTSSPDLLLVIIQVTGVSLLPPLGIAIAVIIIFYCYRVHRQQKLSPSWESSKPRKLMDFSDNCAIILEDDRSDISSTCANNINHNTELLPIELDTLVGKGRFAEVYKAKLKQNTSEQFETVAVKIFPYEEYSSWKTEKDIFSDINLKHENILQFLTAEERKTELGKQYWLITAFHAKGNLQEYLTRHVISWEDLRKLGSSLARGIAHLHSDHTPCGRPKMPIVHRDLKSSNILVKNDLTCCLCDFGLSLRLDPTLSVDDLANSGQVGTARYMAPEVLESRMNLENVESFKQTDVYSMALVLWEMTSRCNAVGEVKDYEPPFGSKVREHPCVESMKDSVLRDRGRPEIPSFWLNHQGIQIVCETLTECWDHDPEARLTAQCVAERFSELEHPERLSGRSCSQEKIPEDGSLNTTK DIP protein Q62312 original sequence version: 121 DIP protein Q62312 original sequence version: 129 1A12D58550921F5E nest_mouse Nestin Nes protein protein mouse Mus musculus Mouse MEGCVGEESFQMWELNRRLEAYLTRVKTLEEQNQLLSAELGGLRAQSGDASWRARADDELAALRVLVDQRWREKHEAEVQRDNLAEELESVAGRCQQVRLARERTIEEAACSRRALEAEKNARGWLSTQAAELERELEALRASHEEERAHLNAQAACTPRRPPAPAHASPIRAPEVEELARRLGEVWRGAVRDYQERVAHMESSLGQARERLGQAVRGARESRLEVQQLQADRDSLQERREALEQRLEGRWQDRLQATEKFQLAVEALEQEKQGLQSQIAQILEGGQQLAHLKMSLSLEVATYRTLLEAENSRLQTPGRSSQASLGFPDPKLKLHFLGIPEDQHLGSVLPVLSPTSFSSPLPNTLETPVTAFLKTQEFLKARTPTLASTPIPPMSEAPYPKNAEVRAQDVPHSLLQGGRQQAPEPLWAEATVPSSTGVLPELEEPGGEQPDHFPDDPTSLAPPLNPHHSILEAKDRESSESRVSSIFQEEEGQIWELVKKEAATEVKVENSLAQEIQESGLDTEEIQDSQGPLQMETLEALGDEPLMSLKTQNHETPGKENCNSSIEENSGTVKSPEKEKQTPLKSLEEKNVEAEKTLENGVLELSKPLGEEEPRMEDQELMSPEHTLETVSFLGKENQEVVRSSEEQNLESLITFKEESQYPLGGPEAEDQMLERLVEKEDQRFPRSPEEDQQAFRPLEKENQEPLRFEEAEDQVLERLIEKERQESLKSPEEEDQQAFRLLEKENQEPLRFEDAEDQVLERLIEKERQESLKSPEEEDQQAFRLLEKENQEPLRFEEAEDQVLERLVEKESQESLKSPEEEDQRTGKPLEKENQESLRSLDENQETIVLLESKNQRPLRSLEVEEEEQRIVKPLEKVSQVSLESLEKENVQSPRYLEEDDHMIKSLLEDKTHEILGSLEDRNGENFIPPENETQGSLRPPEEEDQRIVNHLEKESQEFLRSPEAEEEEEQVMVRSLEGENHDPLSSVVKEEQMAESKLENESQDSRKSLEDESQETFGSLEKENLESLRSLAGQDQEEQKLEQETQQPLRAVEDEQMTVNPPEKVDPELPKPLRNDQEVVRSLDKENQESLVSLNEGGMETVKSSETENIESLETVGECLGRRKSVDTQEPLWSTEVTSETIEPLEDETQEPLGCVDENQEVLTPLERESQELRSLGKWNPETVESPGGVEDSQQCLEVEEGPEREQHQESLRSLGEVEWELPGSGSQQRWEDVVEDGEGQEASLGATGVETEDKAELHLRGQGGEEKAVEEGELLQDAVGEAWSLGSSEPKEQRVPAEPLDDLEGQPEQTGTLEVPVAQGMPEATEQDEDRAQAGEQDSVEVTLGLEAARAGLELEQEVVGLEDPRHFAREEAIHPSLGEESVKAKIDQGLEEPGKEPKEAGALDSGIPELPKTSSETLECKGWEESGEGWGEEEASLETSDHEGSHAPQPRPPKTEEDEGLQAALTVPGPKLLEPCSPIPILTDAHELQPQAEGIQEAGWQPEAGTEALGRVEDEPEFGRGEIPEGLQDWEEGREDSEADELGETLPDSTPLGLYLKSPASPKWEQAGEQRLFPQGEARKEGWSPAALAAQGLSDPPEEEQQGHDSDLSSEEFEDLGTEASLLPGVPKEVSDHLGQEPPVLQPACWDQGGESDGFADEEESGEEGEEEDADEEEGAESGTQWWGPGPSGGGVKVQDVTQRGDLEHESVGDSGLWDDGLSGAAANVLVTALETVSQDSAEPSGSEGSESASLEGEEGQAIDHLDAPQEVTSVVPGAGDTFDISGQGPNLESEQVNGRMENGLEQAEGQVVLHGDEDQGIPLQEQGTLKAPLVGSPVHLGPSQPLKFTLSGVDGDSWSSGED B9DF21005D977983 cntn2_mouse Contactin-2 Axonin-1 Axonal glycoprotein TAG-1 Transient axonal glycoprotein 1 Cntn2 Tax protein protein mouse Mus musculus Mouse MGAPARKRASLLLLLLATMALVSSPGWSFSQGTPATFGPVFEEQPVGLLFPEESAEDQVTLACRARASPPATYRWKMNGTEMNLEPGSRHQLMGGNLVIMSPTKAQDAGVYQCLASNPVGTVVSKEAVLRFGFLQEFSKEERDPVKTHEGWGVMLPCNPPAHYPGLSYRWLLNEFPNFIPTDGRHFVSQTTGNLYIARTNASDLGNYSCLATSHLDFSTKSVFSKFAQLNLAAEDPRLFAPSIKARFPPETYALVGQQVTLECFAFGNPVPRIKWRKVDGSLSPQWGTAEPTLQIPSVSFEDEGTYECEAENSKGRDTVQGRIIVQAQPEWLKVISDTEADIGSNLRWGCAAAGKPRPMVRWLRNGEPLASQNRVEVLAGDLRFSKLNLEDSGMYQCVAENKHGTIYASAELAVQALAPDFRQNPVRRLIPAARGGEISIPCQPRAAPKATILWSKGTEILGNSTRVTVTLDGTLIIRNISRSDEGKYTCFAENFMGKANSTGILSVRDATKITLAPSSADINVGDNLTLQCHASHDPTMDLTFTWTLDDFPVDFDKPGGHYRRASVKETIGDLTILNAQLRHGGTYTCMAQTVVDGASKEATVLVRGPPGPPGGVVVRDIGDTTVQLSWSRGFDNHSPIAKYTLQARTPPSGKWKQVRTNPVNIEGNAETAQVLGLMPWMDYEFRVSASNILGTGEPSGPSSRIRTKEAVPSVAPSGLSGGGGAPGELTINWTPMSREYQNGDGFGYLLSFRRQGSSSWQTARVPGADTQYFVYSNDSIHPYTPFEVKIRSYNRRGDGPESLTAIVYSAEEEPKVAPAKVWAKGSSSSEMNVSWEPVLQDMNGILLGYEIRYWKAGDKEAAADRVRTAGLDSSARVTGLYPNTKYHVTVRAYNRAGTGPASPSADAMTMKPPPRRPPGNISWTFSSSSLSLKWDPVVPLRNESTVTGYKMLYQNDLQPTPMLHLTSKNWIEIPVPEDIGHALVQIRTTGPGGDGIPAEVHIVRNGGTSMMVESSAVRPAHPGPVFSCMVILMLAGCQRL 012C05DDF7F97462 pard3_rat Partitioning defective 3 homolog Pard3 Par3 Atypical PKC isotype-specific-interacting protein Atypical PKC-specific-binding protein protein protein rat Rattus norvegicus (Rat) Rat MKVTVCFGRTRVVVPCGDGRMKVFSLIQQAVTRYRKAVAKDPNYWIQVHRLEHGDGGILDLDDILCDVADDKDRLVAVFDEQDPHHGGDGTSASSTGTQSPEIFGSELGTNNVSAFRPYQTTSEIEVTPSVLRANMPLHVRRSSDPALTGLSTSVSDNNFSSEEPSRKNPTRWSTTAGFLKQNTTGSPKTCDRKKDENYRSLPRDPSSWSNQFQRDNARSSLSASHPMVDRWLEKQEQDEEGTEEDSSRVEPVGHADTGLENMPNFSLDDMVKLVQVPNDGGPLGIHVVPFSARGGRTLGLLVKRLEKGGKAEQENLFHENDCIVRINDGDLRNRRFEQAQHMFRQAMRARVIWFHVVPAANKEQYEQLSQREMNNYSPGRFSPDSHCVANRSVANNAPQALPRAPRLSQPPEQLDAHPRLPHSAHASTKPPTAPALAPPNVLSTSVGSVYNTKRVGKRLNIQLKKGTEGLGFSITSRDVTIGGSAPIYVKNILPRGAAIQDGRLKAGDRLIEVNGVDLAGKSQEEVVSLLRSTKMEGTVSLLVFRQEEAFHPREMNAEPSQMQSPKETKAEDEDIVLTPDGTREFLTFEVPLNDSGSAGLGVSVKGNRSKENHADLGIFVKSIINGGAASKDGRLRVNDQLIAVNGESLLGKANQEAMETLRRSMSTEGNKRGMIQLIVARRISRCNELRSPGSPAAPELPIETELDDRERRISHSLYSGIEGLDESPTRNAALSRIMGESGKCQLSPTVNMPHDDTVMIEDDRLPVLPPHLSDQSSSSSHDDVGFIMTEAGTWAKATISDSADCSLSPDVDPVLAFQREGFGRQSMSEKRTKQFSNASQLDFVKTRKSKSMDLGIADETKLNTVDDQRAGSPNRDVGPSLGLKKSSSLESLQTAVAEVTLNGNIPFHRPRPRIIRGRGCNESFRAAIDKSYDKPMVDDDDEGMETLEEDTEESSRSGRESVSTSSDQPSYSLERQMNGDPEKRDKAEKKKDKAGKDKKKDREKEKDKLKAKKGMLKGLGDMFRFGKHRKDDKMEKMGRIKIQDSFTSEEDRVRMKEEQERIQAKTREFRERQARERDYAEIQDFHRTFGCDDELLYGGMSSYDGCLALNARPQSPREGHLMDTLYAQVKKPRSSKPGDSNRSTPSNHDRIQRLRQEFQQAKQDEDVEDRRRTYSFEQSWSSSRPASQSGRHSVSVEVQVQRQRQEERESFQQAQRQYSSLPRQSRKNASSVSQDSWEQNYAPGEGFQSAKENPRYSSYQGSRNGYLGGHGFNARVMLETQELLRQEQRRKEQQLKKQPPADGVRGPFRQDVPPSPSQVARLNRLQTPEKGRPFYS DIP protein Q9Z340 original sequence version: 109 EC980C5106B52F9C par6a_mouse Partitioning defective 6 homolog alpha Pard6a Par6a protein protein mouse Mus musculus Mouse MARPQRTPARSPDSIVEVKSKFDAEFRRFALPRTSVRGFQEFSRLLCVVHQIPGLDVLLGYTDAHGDLLPLTNDDSLHRALASGPPPLRLLVQKRAEGDSSGLAFASNSLQRRKKGLLLRPVAPLRTRPPLLISLPQDFRQVSSVIDVDLLPETHRRVRLHKHGSDRPLGFYIRDGMSVRVAPQGLERVPGIFISRLVRGGLAESTGLLAVSDEILEVNGIEVAGKTLDQVTDMMVANSHNLIVTVKPANQRNNVVRGASGRLTGPSSVGPGPTDPDSDDDSSDLVIENRHPPCSNGLSQGPLCWDLQPGCLLPGAGSSLPSLDSREQANSGWGNGMRGDVSGFSL DIP protein Q9Z101 original sequence version: 97 8F150B83B7E1F36A tgfr2_rat TGF-beta receptor type-2 Tgfbr2 Transforming growth factor-beta receptor type II TGF-beta type II receptor protein protein rat Rattus norvegicus (Rat) Rat MGRGLLRGLWPLHIVLWTRIASTIPPHVPKSVNSDLMAGDNSGAVKLPQLCKFCDVTLSTCDNQKSCMSNCSVTSICEKPQEVCVAVWRKNDKNITLETVCHDPKFTYHGFTLEDATSPTCVMKEKKRAGETFFMCSCNTEECNDYIIFNEEYTTSSPDLLLVIIQVTGVSLLPPLGIAIAVIAIFYCYRVHRQQKLSPSWESSKPRKLMDFSDNCAIILEDDRSDISSTCANNINHNTELLPIELDTLVGKGRFAEVYKAKLKQNTSEQFETVAVKIFPYEEYSSWKTEKDIFSDINLKHENILQFLTAEERKTEMGKQYWLITAFHAKGNLQEYLTRHVISWEDLRKLGSSLARGIAHLHSDHTPCGRPKMPIVHRDLKSSNILVKNDLTCCLCDFGLSLRLDPTLSVDDLANSGQVGTARYMAPEVLESRMNLENMESFKQTDVYSMALVLWEMTSRCNAVGEVKDYEPPFGSKVREHPCVESMKDNVLRDRGRPEIPSFWLNHQGIQIVCETLTECWDHDPEARLTAQCVAERFSELEHPDRLSGRSCSQEKIPEDGSLNTTK EC1D7642A51A3B75 tbr1-par 3 11 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Fig 6E. Both Par6 and Par3 coimmunoprecipitated with TBR1 from E18 rat forebrain lysates, indicating that TBR1 and Par3/Par6 form a biochemical complex in developing brain. tbr2-tag1 1 10 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Simultaneously labelling of the cortex with an antibody for TBR2 and TAG1, (a marker of corticofugal axons) showed coincident immunoreactivity between TBR2 and TAG1. Fig 1 E+F. there does not seem to be an entry for 'corticofugal axon' in GO. tgfr1_2-nestin 1 5 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component colocalization colocalization Both TBR1 and TBR2 are highly expressed throughout the mouse neocortex, including nestin-positive radial glial progenitors. Both receptor types are present at apical domains of radial glia. Fig 1 A+B. Not sure if any Xrefs are appropriate:'radial glial cell (progenitor)' is not a GO term - (only a "CL, TAO or ZFA" term - TAO/ZFA are for fish only!.) tgfr1_2 1 8 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component colocalization colocalization Both TBR1 and TBR2 were found in the cell bodies of layer 6 neurons, and diffuse TBR1 labeling was found within the intermediate zone (IZ) of the cortical wall. Fig 1 C+D. I cannot see if Tuj1 is co-localised or not on the (1C)image - so have not entered either as the text does not clarify. (it is a B-tubulin marker only). tgfr1_2-1 4 7 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component colocalization colocalization Punctate distribution of TBR1 and TBR2 at the tips of all neurites can be seen in stage 2 cells prior to polarization. By stage 3 (DIV4), TBR1and TBR2 were enriched within the within the growth cone of the axon and largely absent from dendrites. Fig 3 D-F. tgfr1_2-nestin-par6 2 12 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component colocalization colocalization Using antibody labeled brain slices from E18 mouse embryos both Par6 and TBR1 are enriched within apical domains of radial glia in the VZ. Fig 6 A+B. Not sure if any Xrefs are appropriate:'radial glial cell (progenitor)' is not a GO term - (only a "CL, TAO or ZFA" term - TAO/ZFA are for fish only!.) tgfr1-par6 4 6 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization In dissociated hippocampal neurons, surface TBR1 and Par6 colocalize in discrete punctate clusters within growth cones of undifferentiated neurites, particularly within filopodial protrusions. Fig 6 C+D. I have left in "filopodium" as the definition contains this: "Thin, stiff protrusion extended by the leading edge of a motile cell such as a crawling fibroblast or amoeba, or an axonal growth cone;" (the number of GO terms might be OTT)