Molecular Connections Molecular Connections Pvt. Ltd. 2/2, Kariappa Road, Basavanagudi, Bangalore -560 004 www.molecularconnections.com A novel mechanism of cell growth regulation by Cell Cycle and Apoptosis Regulatory Protein (CARP)-1. A novel mechanism of cell growth regulation by Cell Cycle and Apoptosis Regulatory Protein (CARP)-1. J Mol Signal 2010 imex curation Jiang Y., Puliyappadamba VT., Zhang L., Wu W., Wali A., Yaffe MB., Fontana JA., Rishi AK. Apoptosis - Interactions involving proteins with a function related to apoptosis Rishia@Karmanos.org 21-Dec-2010: Contacted by JYOTI. mgt Only protein-protein interactions Curation by: Molecular Connections Pvt. Ltd human-mda_mb_468 Homo sapiens MDA-MB-468 mda_mb_468 MDA-MB-468 breast adenocarcinoma Species: human, Black; Tissue: breast; Tumor: adenocarcinoma; Derived from: pleural effusion anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jiang Y., Puliyappadamba VT., Zhang L., Wu W., Wali A., Yaffe MB., Fontana JA., Rishi AK. Apoptosis - Interactions involving proteins with a function related to apoptosis Rishia@Karmanos.org. Rishia@Karmanos.org Accepted 2010-OCT-30 by MGT. 21-Dec-2010: Contacted by JYOTI. J Mol Signal 2010 mgt Only protein-protein interactions imex curation Curation by: Molecular Connections Pvt. Ltd A novel mechanism of cell growth regulation by Cell Cycle and Apoptosis Regulatory Protein (CARP)-1. A novel mechanism of cell growth regulation by Cell Cycle and Apoptosis Regulatory Protein (CARP)-1. J Mol Signal 2010 imex curation Jiang Y., Puliyappadamba VT., Zhang L., Wu W., Wali A., Yaffe MB., Fontana JA., Rishi AK. Apoptosis - Interactions involving proteins with a function related to apoptosis Rishia@Karmanos.org 21-Dec-2010: Contacted by JYOTI. mgt Only protein-protein interactions Curation by: Molecular Connections Pvt. Ltd chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Jiang Y., Puliyappadamba VT., Zhang L., Wu W., Wali A., Yaffe MB., Fontana JA., Rishi AK. Apoptosis - Interactions involving proteins with a function related to apoptosis Rishia@Karmanos.org. Rishia@Karmanos.org Accepted 2010-OCT-30 by MGT. 21-Dec-2010: Contacted by JYOTI. J Mol Signal 2010 mgt Only protein-protein interactions imex curation Curation by: Molecular Connections Pvt. Ltd A novel mechanism of cell growth regulation by Cell Cycle and Apoptosis Regulatory Protein (CARP)-1. A novel mechanism of cell growth regulation by Cell Cycle and Apoptosis Regulatory Protein (CARP)-1. J Mol Signal 2010 imex curation Jiang Y., Puliyappadamba VT., Zhang L., Wu W., Wali A., Yaffe MB., Fontana JA., Rishi AK. Apoptosis - Interactions involving proteins with a function related to apoptosis Rishia@Karmanos.org 21-Dec-2010: Contacted by JYOTI. mgt Only protein-protein interactions Curation by: Molecular Connections Pvt. Ltd chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fluorescence imaging fluorescence microscopy fluorescence imaging anti tag immunost anti tag immunostaining anti tag immunost Jiang Y., Puliyappadamba VT., Zhang L., Wu W., Wali A., Yaffe MB., Fontana JA., Rishi AK. Apoptosis - Interactions involving proteins with a function related to apoptosis Rishia@Karmanos.org. Rishia@Karmanos.org Accepted 2010-OCT-30 by MGT. 21-Dec-2010: Contacted by JYOTI. J Mol Signal 2010 mgt Only protein-protein interactions imex curation Curation by: Molecular Connections Pvt. Ltd A novel mechanism of cell growth regulation by Cell Cycle and Apoptosis Regulatory Protein (CARP)-1. A novel mechanism of cell growth regulation by Cell Cycle and Apoptosis Regulatory Protein (CARP)-1. J Mol Signal 2010 imex curation Jiang Y., Puliyappadamba VT., Zhang L., Wu W., Wali A., Yaffe MB., Fontana JA., Rishi AK. Apoptosis - Interactions involving proteins with a function related to apoptosis Rishia@Karmanos.org 21-Dec-2010: Contacted by JYOTI. mgt Only protein-protein interactions Curation by: Molecular Connections Pvt. Ltd human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Jiang Y., Puliyappadamba VT., Zhang L., Wu W., Wali A., Yaffe MB., Fontana JA., Rishi AK. Apoptosis - Interactions involving proteins with a function related to apoptosis Rishia@Karmanos.org. Rishia@Karmanos.org Accepted 2010-OCT-30 by MGT. 21-Dec-2010: Contacted by JYOTI. J Mol Signal 2010 mgt Only protein-protein interactions imex curation Curation by: Molecular Connections Pvt. Ltd A novel mechanism of cell growth regulation by Cell Cycle and Apoptosis Regulatory Protein (CARP)-1. A novel mechanism of cell growth regulation by Cell Cycle and Apoptosis Regulatory Protein (CARP)-1. J Mol Signal 2010 imex curation Jiang Y., Puliyappadamba VT., Zhang L., Wu W., Wali A., Yaffe MB., Fontana JA., Rishi AK. Apoptosis - Interactions involving proteins with a function related to apoptosis Rishia@Karmanos.org 21-Dec-2010: Contacted by JYOTI. mgt Only protein-protein interactions Curation by: Molecular Connections Pvt. Ltd human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jiang Y., Puliyappadamba VT., Zhang L., Wu W., Wali A., Yaffe MB., Fontana JA., Rishi AK. Apoptosis - Interactions involving proteins with a function related to apoptosis Rishia@Karmanos.org. Rishia@Karmanos.org Accepted 2010-OCT-30 by MGT. 21-Dec-2010: Contacted by JYOTI. J Mol Signal 2010 mgt Only protein-protein interactions imex curation Curation by: Molecular Connections Pvt. Ltd ccar1_human Cell division cycle and apoptosis regulator protein 1 CCAR1 CARP1 DIS Cell cycle and apoptosis regulatory protein 1 Death inducer with SAP domain protein protein human Homo sapiens Human MAQFGGQKNPPWATQFTATAVSQPAALGVQQPSLLGASPTIYTQQTALAAAGLTTQTPANYQLTQTAALQQQAAAAAAALQQQYSQPQQALYSVQQQLQQPQQTLLTQPAVALPTSLSLSTPQPTAQITVSYPTPRSSQQQTQPQKQRVFTGVVTKLHDTFGFVDEDVFFQLSAVKGKTPQVGDRVLVEATYNPNMPFKWNAQRIQTLPNQNQSQTQPLLKTPPAVLQPIAPQTTFGVQTQPQPQSLLQAQISAASITPLLQTQPQPLLQQPQQKAGLLQPPVRIVSQPQPARRLDPPSRFSGRNDRGDQVPNRKDDRSRERERERRRSRERSPQRKRSRERSPRRERERSPRRVRRVVPRYTVQFSKFSLDCPSCDMMELRRRYQNLYIPSDFFDAQFTWVDAFPLSRPFQLGNYCNFYVMHREVESLEKNMAILDPPDADHLYSAKVMLMASPSMEDLYHKSCALAEDPQELRDGFQHPARLVKFLVGMKGKDEAMAIGGHWSPSLDGPDPEKDPSVLIKTAIRCCKALTGIDLSVCTQWYRFAEIRYHRPEETHKGRTVPAHVETVVLFFPDVWHCLPTRSEWETLSRGYKQQLVEKLQGERKEADGEQDEEEKDDGEAKEISTPTHWSKLDPKTMKVNDLRKELESRALSSKGLKSQLIARLTKQLKVEEQKEEQKELEKSEKEEDEDDDRKSEDDKEEEERKRQEEIERQRRERRYILPDEPAIIVHPNWAAKSGKFDCSIMSLSVLLDYRLEDNKEHSFEVSLFAELFNEMLQRDFGVRIYKSLLSLPEKEDKKEKDKKSKKDERKDKKEERDDETDEPKPKRRKSGDDKDKKEDRDERKKEDKRKDDSKDDDETEEDNNQDEYDPMEAEEAEDEEDDRDEEEMTKRDDKRDINRYCKERPSKDKEKEKTQMITINRDLLMAFVYFDQSHCGYLLEKDLEEILYTLGLHLSRAQVKKLLNKVVLRESCFYRKLTDTSKDEENHEESESLQEDMLGNRLLLPTPTVKQESKDVEENVGLIVYNGAMVDVGSLLQKLEKSEKVRAEVEQKLQLLEEKTDEDEKTILNLENSNKSLSGELREVKKDLSQLQENLKISENMNLQFENQMNKTIRNLSTVMDEIHTVLKKDNVKNEDKDQKSKENGASV 30618DCC8097F552 wwtr1_human WW domain-containing transcription regulator protein 1 Transcriptional coactivator with PDZ-binding motif WWTR1 TAZ protein protein human Homo sapiens Human MNPASAPPPLPPPGQQVIHVTQDLDTDLEALFNSVMNPKPSSWRKKILPESFFKEPDSGSHSRQSSTDSSGGHPGPRLAGGAQHVRSHSSPASLQLGTGAGAAGSPAQQHAHLRQQSYDVTDELPLPPGWEMTFTATGQRYFLNHIEKITTWQDPRKAMNQPLNHMNLHPAVSSTPVPQRSMAVSQPNLVMNHQHQQQMAPSTLSQQNHPTQNPPAGLMSMPNALTTQQQQQQKLRLQRIQMERERIRMRQEELMRQEAALCRQLPMEAETLAPVQAAVNPPTMTPDMRSITNNSSDPFLNGGPYHSREQSTDSGLGLGCYSVPTTPEDFLSNVDEMDTGENAGQTPMNINPQQTRFPDFLDCLPGTNVDLGTLESEDLIPLFNDVESALNKSEPFLTWL 630B50F46FB74C60 wwtr1_mouse WW domain-containing transcription regulator protein 1 Wwtr1 Taz Transcriptional coactivator with PDZ-binding motif protein protein mouse Mus musculus Mouse MNPSSVPHPLPPPGQQVIHVTQDLDTDLEALFNSVMNPKPSSWRKKILPESFFKEPDSGSHSRQSSTDSSGGHPGPRLAGGAQHVRSHSSPASLQLGTGAGAAGGPAQQHAHLRQQSYDVTDELPLPPGWEMTFTATGQRYFLNHIEKITTWQDPRKVMNQPLNHVNLHPSITSTSVPQRSMAVSQPNLAMNHQHQQVVATSLSPQNHPTQNQPTGLMSVPNALTTQQQQQQKLRLQRIQMERERIRMRQEELMRQEAALCRQLPMETETMAPVNTPAMSTDMRSVTNSSSDPFLNGGPYHSREQSTDSGLGLGCYSVPTTPEDFLSNMDEMDTGENSGQTPMTVNPQQTRFPDFLDCLPGTNVDLGTLESEDLIPLFNDVESALNKSEPFLTWL DIP protein Q9EPK5 original sequence version: 112 1B8A7487D4CA341C carp1-taz-1 1 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association fig. 3a carp1-taz-2 5 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association fig. 3a taz-carp1 4 6 unspecified role unspecified role prey prey c-term myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-term his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait n-terminal position flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association fig. 3b carp1-taz-4 3 6 unspecified role unspecified role neutral component neutral component c-term his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-term myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role neutral component neutral component n-terminal position flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization fig. 3d taz-carp1-2 2 6 unspecified role unspecified role prey prey c-term myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-term his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait n-term flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Additional file 1a carp1-taz-6 2 6 unspecified role unspecified role bait bait c-term myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-term his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role prey prey n-term flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Additional file 1a taz-carp1-1 2 6 unspecified role unspecified role prey prey c-term myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q8IX12:p.Thr667Asp mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain T D Wed May 02 20:12:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr667asp' Tue Jun 30 16:28:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr667Asp' Q8IX12:p.Thr667Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain T A Wed May 02 17:41:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr667ala' Tue Jun 30 16:26:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr667Ala' c-term his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait n-terminal position gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association additional file 1b and fig. 5b