IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Modulation of the vitamin D3 response by cancer-associated mutant p53. Modulation of the vitamin D3 response by cancer-associated mutant p53. Cancer Cell (1535-6108) 2010 imex curation Stambolsky P., Tabach Y., Fontemaggi G., Weisz L., Maor-Aloni R., Sigfried Z., Shiff I., Kogan I., Shay M., Kalo E., Blandino G., Simon I., Oren M., Rotter V. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Hu13K human promoter array was used. perrystam@hotmail.com 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 5 human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Stambolsky P., Tabach Y., Fontemaggi G., Weisz L., Maor-Aloni R., Sigfried Z., Shiff I., Kogan I., Shay M., Kalo E., Blandino G., Simon I., Oren M., Rotter V. Apoptosis - Interactions involving proteins with a function related to apoptosis Hu13K human promoter array was used. perrystam@hotmail.com Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 5 imex curation Modulation of the vitamin D3 response by cancer-associated mutant p53. Modulation of the vitamin D3 response by cancer-associated mutant p53. Cancer Cell (1535-6108) 2010 imex curation Stambolsky P., Tabach Y., Fontemaggi G., Weisz L., Maor-Aloni R., Sigfried Z., Shiff I., Kogan I., Shay M., Kalo E., Blandino G., Simon I., Oren M., Rotter V. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Hu13K human promoter array was used. perrystam@hotmail.com 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 5 human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Stambolsky P., Tabach Y., Fontemaggi G., Weisz L., Maor-Aloni R., Sigfried Z., Shiff I., Kogan I., Shay M., Kalo E., Blandino G., Simon I., Oren M., Rotter V. Apoptosis - Interactions involving proteins with a function related to apoptosis Hu13K human promoter array was used. perrystam@hotmail.com Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 5 imex curation Modulation of the vitamin D3 response by cancer-associated mutant p53. Modulation of the vitamin D3 response by cancer-associated mutant p53. Cancer Cell (1535-6108) 2010 imex curation Stambolsky P., Tabach Y., Fontemaggi G., Weisz L., Maor-Aloni R., Sigfried Z., Shiff I., Kogan I., Shay M., Kalo E., Blandino G., Simon I., Oren M., Rotter V. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Hu13K human promoter array was used. perrystam@hotmail.com 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 5 human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion filter binding filter binding Filter overlay assay Filter overlay assay western blot western blot Immuno blot Immuno blot Stambolsky P., Tabach Y., Fontemaggi G., Weisz L., Maor-Aloni R., Sigfried Z., Shiff I., Kogan I., Shay M., Kalo E., Blandino G., Simon I., Oren M., Rotter V. Apoptosis - Interactions involving proteins with a function related to apoptosis Hu13K human promoter array was used. perrystam@hotmail.com Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 5 imex curation Modulation of the vitamin D3 response by cancer-associated mutant p53. Modulation of the vitamin D3 response by cancer-associated mutant p53. Cancer Cell (1535-6108) 2010 imex curation Stambolsky P., Tabach Y., Fontemaggi G., Weisz L., Maor-Aloni R., Sigfried Z., Shiff I., Kogan I., Shay M., Kalo E., Blandino G., Simon I., Oren M., Rotter V. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Hu13K human promoter array was used. perrystam@hotmail.com 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 5 human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion chip-chip chromatin immunoprecipitation array chip-chip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Stambolsky P., Tabach Y., Fontemaggi G., Weisz L., Maor-Aloni R., Sigfried Z., Shiff I., Kogan I., Shay M., Kalo E., Blandino G., Simon I., Oren M., Rotter V. Apoptosis - Interactions involving proteins with a function related to apoptosis Hu13K human promoter array was used. perrystam@hotmail.com Accepted 2010-MAY-12 by ORCHARD. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 5 imex curation Modulation of the vitamin D3 response by cancer-associated mutant p53. Modulation of the vitamin D3 response by cancer-associated mutant p53. Cancer Cell (1535-6108) 2010 imex curation Stambolsky P., Tabach Y., Fontemaggi G., Weisz L., Maor-Aloni R., Sigfried Z., Shiff I., Kogan I., Shay M., Kalo E., Blandino G., Simon I., Oren M., Rotter V. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Hu13K human promoter array was used. perrystam@hotmail.com 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 5 human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Stambolsky P., Tabach Y., Fontemaggi G., Weisz L., Maor-Aloni R., Sigfried Z., Shiff I., Kogan I., Shay M., Kalo E., Blandino G., Simon I., Oren M., Rotter V. Apoptosis - Interactions involving proteins with a function related to apoptosis Hu13K human promoter array was used. perrystam@hotmail.com Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 5 imex curation Modulation of the vitamin D3 response by cancer-associated mutant p53. Modulation of the vitamin D3 response by cancer-associated mutant p53. Cancer Cell (1535-6108) 2010 imex curation Stambolsky P., Tabach Y., Fontemaggi G., Weisz L., Maor-Aloni R., Sigfried Z., Shiff I., Kogan I., Shay M., Kalo E., Blandino G., Simon I., Oren M., Rotter V. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Hu13K human promoter array was used. perrystam@hotmail.com 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 5 human-mda_mb_231 Homo sapiens Caucasian breast adenocarcinoma mda_mb_231 Human Caucasian breast adenocarcinoma ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Stambolsky P., Tabach Y., Fontemaggi G., Weisz L., Maor-Aloni R., Sigfried Z., Shiff I., Kogan I., Shay M., Kalo E., Blandino G., Simon I., Oren M., Rotter V. Apoptosis - Interactions involving proteins with a function related to apoptosis Hu13K human promoter array was used. perrystam@hotmail.com Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 5 imex curation Modulation of the vitamin D3 response by cancer-associated mutant p53. Modulation of the vitamin D3 response by cancer-associated mutant p53. Cancer Cell (1535-6108) 2010 imex curation Stambolsky P., Tabach Y., Fontemaggi G., Weisz L., Maor-Aloni R., Sigfried Z., Shiff I., Kogan I., Shay M., Kalo E., Blandino G., Simon I., Oren M., Rotter V. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Hu13K human promoter array was used. perrystam@hotmail.com 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 5 human-skbr3 Homo sapiens SKBR3 skbr3 SKBR3 Species: human; Tissue: breast; Tumor: adenocarcinoma; Derived from: metastatic site: pleural effusion anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Stambolsky P., Tabach Y., Fontemaggi G., Weisz L., Maor-Aloni R., Sigfried Z., Shiff I., Kogan I., Shay M., Kalo E., Blandino G., Simon I., Oren M., Rotter V. Apoptosis - Interactions involving proteins with a function related to apoptosis Hu13K human promoter array was used. perrystam@hotmail.com Accepted 2010-MAY-12 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 5 imex curation klf10_human_gene Human KLF10 promoter gene gene human Homo sapiens Human lig1_human_gene Human LIG1 promoter gene gene human Homo sapiens Human cp24a_human_gene Human CYP24A1 promoter VDRE (Vitamin D Response Element) gene gene human Homo sapiens Human skp2_human_gene Human SKP2 promoter gene gene human Homo sapiens Human nfs1_human_gene Human NFS1 gene gene gene human Homo sapiens Human fras1_human_gene Human FRAS1 promoter gene gene human Homo sapiens Human cpt1b_human _gene Human CPT1B (muscle isoform) promoter gene gene human Homo sapiens Human SwissProt entry is Q92523 ece2_human_gene Human ECE2 promoter gene gene human Homo sapiens Human mkl2_human_gene Human MKL2 gene gene gene human Homo sapiens Human pcdha7_human_gene Human PCDHA7 promoter gene gene human Homo sapiens Human vipr2_human_gene Human VIPR2 promoter gene gene human Homo sapiens Human cite1_human Cbp/p300-interacting transactivator 1 CITED1 MSG1 Melanocyte-specific protein 1 protein protein human Homo sapiens Human MPTTSRPALDVKGGTSPAKEDANQEMSSVAYSNLAVKDRKAVAILHYPGVASNGTKASGAPTSSSGSPIGSPTTTPPTKPPSFNLHPAPHLLASMHLQKLNSQYQGMAAATPGQPGEAGPLQNWDFGAQAGGAESLSPSAGAQSPAIIDSDPVDEEVLMSLVVELGLDRANELPELWLGQNEFDFTADFPSSC 9B0CF50CA639ECCB tgfb1_human_gene TGFB1_HUMAN_GENE gene gene human Homo sapiens Human mageh1_human_gene Human MAGEH1 promoter gene gene human Homo sapiens Human c12orf49_human_gene Human C12orf49 promoter gene gene human Homo sapiens Human vdr_human Vitamin D3 receptor 1,25-dihydroxyvitamin D3 receptor Nuclear receptor subfamily 1 group I member 1 VDR NR1I1 protein protein human Homo sapiens Human MEAMAASTSLPDPGDFDRNVPRICGVCGDRATGFHFNAMTCEGCKGFFRRSMKRKALFTCPFNGDCRITKDNRRHCQACRLKRCVDIGMMKEFILTDEEVQRKREMILKRKEEEALKDSLRPKLSEEQQRIIAILLDAHHKTYDPTYSDFCQFRPPVRVNDGGGSHPSRPNSRHTPSFSGDSSSSCSDHCITSSDMMDSSSFSNLDLSEEDSDDPSVTLELSQLSMLPHLADLVSYSIQKVIGFAKMIPGFRDLTSEDQIVLLKSSAIEVIMLRSNESFTMDDMSWTCGNQDYKYRVSDVTKAGHSLELIEPLIKFQVGLKKLNLHEEEHVLLMAICIVSPDRPGVQDAALIEAIQDRLSNTLQTYIRCRHPPPGSHLLYAKMIQKLADLRSLNEEHSKQYRCLSFQPECSMKLTPLVLEVFGNEIS DIP protein P11473 original sequence version: 179 DIP protein P11473 original sequence version: 146 F95F300D042C4CB7 p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 tuba1c_human_gene Human TUBA1C promoter gene gene human Homo sapiens Human mark2_human_gene Human MARK2 promoter gene gene human Homo sapiens Human ecrg4_human_gene Human ECRG4 promoter gene gene human Homo sapiens Human cp27b_human_gene Human CYP27B1 promoter nVDRE (negative Vitamin D Response Element) gene gene human Homo sapiens Human vdre_probe_human Human Vitamin D Response Element VDRE: consensus oligonucleotide dna deoxyribonucleic acid DNA deoxyribonucleic acid dna DNA deoxyribonucleic acid human Homo sapiens Human AGGTCANNNAGGTCA VDRE consensus oligonucleotide sequence. 0778F6672BB11F41 hira_human_gene Human HIRA promoter VDRE (Vitamin D Response Element) gene gene human Homo sapiens Human stath_human_gene Human STATH promoter gene gene human Homo sapiens Human actr10_human_gene Human ACTR10 promoter gene gene human Homo sapiens Human ghr_human_gene Human Growth Hormone Receptor Promoter gene gene human Homo sapiens Human mutp53-vipr2 4 18 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 17:38:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 18:31:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). mutp53-mkl2 4 16 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 14:26:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 18:27:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). mutp53-mageh1 4 21 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 17:53:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 17:46:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). mutp53-ece2 4 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 20:02:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 16:00:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' 15 unspecified role unspecified role prey prey association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). mutp53-fras1 4 13 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 20:03:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 20:14:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). mutp53-ghr 4 33 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 17:16:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 18:55:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). mutp53-mark2 4 26 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 15:14:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 18:41:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). mutp53-cpt1b 4 14 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 19:18:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 17:54:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). mutp53-skp2 4 11 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 16:40:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 18:34:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). mutp53-nfs1 4 12 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 17:21:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 16:04:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). mutp53-ecrg4 4 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 14:31:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 16:31:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' 27 unspecified role unspecified role prey prey association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). mutp53-klf10 4 8 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 19:08:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 17:50:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). mutp53-actr10 4 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 18:10:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 16:22:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' 32 unspecified role unspecified role prey prey association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). mutp53-lig1 4 9 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 19:39:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 18:12:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). mutp53-stath 4 31 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 17:51:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 16:10:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). mutp53-tuba1c 4 25 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 15:10:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 18:28:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). mutp53-c12orf49 4 22 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 20:20:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 16:53:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). mutp53-pcdha7 4 17 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 14:46:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 19:11:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Table 1 One of thirty Gene Promoters preferentially Bound by Mutp53(R175H). vdre-mutp53 3 29 unspecified role unspecified role bait bait 24 unspecified role unspecified role prey prey P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 14:45:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 18:28:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' physical association physical association Fig S1B. mutp53 displayed weak specific binding to the VDRE under basal conditions, which was significantly augmented upon vitamin D3 treatment. 100nM vitamin D3 vdre-vdr 3 29 unspecified role unspecified role bait bait 23 unspecified role unspecified role prey prey physical association physical association Fig S1B. Vitamin D3 treatment augmented the binding of VDR to a wild-type but not mutant VDRE sequence, as expected. 100nM vitamin D3 vdr-mutp53 2 23 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 18:28:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 19:17:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' colocalization colocalization Fig 2D. Zn2+ (to induce p53 expression). Vitamin D3. In untreated H1299R175H-i cells VDR is largely cytoplasmic. Combining mutp53 induction with vitamin D3 rendered VDR staining more prominent and almost exclusively nuclear. vdr-mutp53-1 7 23 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 16:51:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 20:16:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' physical association physical association Fig 3A. Vitamin D3 Reciprocal coimmunoprecipitations carried out with either a mutp53-specific antibody or anti-VDR antibody. vdr-mutp53-2 7 24 unspecified role unspecified role prey prey P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 16:35:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 16:32:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' 23 unspecified role unspecified role bait bait physical association physical association Fig 3A, Fig S2A. Vitamin D3 Pull-down of VDR coprecipitated p53 from both SKBR3 and MCF7 cell extracts. The coimmunoprecipitation of mutp53 with VDR was further augmented when a wild-type, but not mutant, VDRE consensus oligonucleotide was added to the cell extract during incubation, indicating that the VDRE sequence can recruit both proteins simultaneously. Wild type VDRE consensus oligonucleotide vdr-mutp53-3 1 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 16:17:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 17:12:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' 23 unspecified role unspecified role prey prey physical association physical association Fig 3A. Vitamin D3 Reciprocal coimmunoprecipitations carried out with either a mutp53-specific antibody or anti-VDR antibody. vdr-mutp53-4 1 24 unspecified role unspecified role prey prey 23 unspecified role unspecified role bait bait physical association physical association Fig 3A. Vitamin D3 Pull-down of VDR coprecipitated p53 from both SKBR3 and MCF7 cell extracts. vdr-hira 5 30 unspecified role unspecified role prey prey 23 unspecified role unspecified role bait bait association association Fig 1A. Vitamin D3 Significant binding of mutp53 to the VDRE-containing regions of both HIRA and TGF-B1 promoters was clearly detectable, and Vitamin D3 treatment markedly increased binding. p53mut-hira 5 30 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 16:17:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 17:27:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Fig 1A. Vitamin D3 Significant binding of mutp53 to the VDRE-containing regions of both HIRA and TGF-B1 promoters was clearly detectable, and Vitamin D3 treatment markedly increased binding. p53mut-tgfb1 5 20 unspecified role unspecified role prey prey promoter vdre (vitamin d response element) binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 18:10:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 19:14:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Fig 1A. Vitamin D3 Significant binding of mutp53 to the VDRE-containing regions of both HIRA and TGF-B1 promoters was clearly detectable, and Vitamin D3 treatment markedly increased binding. vdr-tgfb1 5 20 unspecified role unspecified role prey prey promoter vdre (vitamin d response element) binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 23 unspecified role unspecified role bait bait association association Fig 1A. Vitamin D3 Significant binding of mutp53 to the VDRE-containing regions of both HIRA and TGF-B1 promoters was clearly detectable, and Vitamin D3 treatment markedly increased binding. vdr-cp24a 5 10 unspecified role unspecified role prey prey 23 unspecified role unspecified role bait bait association association Fig 3D. Vitamin D3 mutp53, VDR, and p300 bound to the CYP24A1 promoter VDRE. p53mut-cyp24a 5 10 unspecified role unspecified role prey prey 24 unspecified role unspecified role bait bait P04637:p.Arg175His mutation mutation certain certain sequence position certain certain certain sequence position certain R H Wed May 02 17:09:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 16:57:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' association association Fig 3D. Vitamin D3 mutp53, VDR, and p300 bound to the CYP24A1 promoter VDRE. p300-cyp24a-1 5 19 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey association association Fig 3D. Vitamin D3 mutp53, VDR, and p300 bound to the CYP24A1 promoter VDRE. vdr-cp24a-p53 6 23 unspecified role unspecified role bait bait 24 unspecified role unspecified role prey prey P04637:p.Arg280Lys mutation mutation certain certain sequence position certain certain certain sequence position certain R K Wed May 02 16:55:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg280lys' Tue Jun 30 16:39:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg280Lys' 10 unspecified role unspecified role prey prey association association Fig 3E. Vitamin D3 ChIP for p53 followed by re-ChIP for VDR or p300 enriched for the presence of the CYP24A VDRE when treated with vitamin D3. p300-cyp24a-p53 6 24 unspecified role unspecified role prey prey P04637:p.Arg280Lys mutation mutation certain certain sequence position certain certain certain sequence position certain R K Wed May 02 20:22:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg280lys' Tue Jun 30 16:36:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg280Lys' 10 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig 3E. Vitamin D3 ChIP for p53 followed by re-ChIP for VDR or p300 enriched for the presence of the CYP24A VDRE when treated with vitamin D3. cite1-cp27b 5 28 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait association association Fig 3G. Vitamin D3 Mutp53 was not associated with the region of the CYP27B gene harboring the nVDRE.