IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. Cancer Cell (1535-6108) 2010 imex curation Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 31 human-hb1119 Homo sapiens acute lymphoblastic leukemia cell line HB1119 hb1119 Human acute lymphoblastic leukemia cell line HB1119 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Trembl entries for fusion proteins are given as a reference only. EBI-2614786: looks OK-ish to me - there should be 2 entries for this cell line, and the preys look correct for this interaction after altering the fusion protein IDS. I have only entered an interaction when I can see a distinct band on the image.(some are not very clear as to wether there is a band of not...nothing is stated regarding presence/absence of specific bands in the text, for the most part) Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu Accepted 2010-APR-24 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 31 imex curation A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. Cancer Cell (1535-6108) 2010 imex curation Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 31 human-kp-l-ry Human: KP-L-RY leukemic blast cell line kp-l-ry Human KP-L-RY leukemic blast cell line Lymphoblast cell line obtained from the peripheral blood of an ALL infant with MLL rearrangement. In the fusion transcript the MLL exon 6 is fused in frame to the 5' side of the putative transactivation domain of AF5q31. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Trembl entries for fusion proteins are given as a reference only. Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu Accepted 2010-APR-24 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 31 imex curation A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. Cancer Cell (1535-6108) 2010 imex curation Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 31 human-ml_2 Homo sapiens ML-2 acute myeloid leukemia-derived cell line ml_2 Human acute myeloid leukemia-derived cell line ML-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu Accepted 2010-APR-24 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 31 imex curation A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. Cancer Cell (1535-6108) 2010 imex curation Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 31 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu Accepted 2010-APR-24 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 31 imex curation A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. Cancer Cell (1535-6108) 2010 imex curation Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 31 human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom ms participant Identification by mass spectrometry ms participant Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu Accepted 2010-APR-24 by ORCHARD. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 31 imex curation A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. Cancer Cell (1535-6108) 2010 imex curation Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 31 human-ml_2 Homo sapiens ML-2 acute myeloid leukemia-derived cell line ml_2 Human acute myeloid leukemia-derived cell line ML-2 affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom ms participant Identification by mass spectrometry ms participant Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu Accepted 2010-APR-24 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 31 imex curation A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. Cancer Cell (1535-6108) 2010 imex curation Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 31 human-sem Human acute lymphoblastic leukemia cell line SEM sem Human acute lymphoblastic leukemia cell line SEM SEM-K2 cells express amplified wild-type FLT3 along with the MLL-AF4 fusion protein. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Cell line used was SEM-K2 -a subclone of the SEM cell line. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu Accepted 2010-APR-24 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 31 imex curation A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. Cancer Cell (1535-6108) 2010 imex curation Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 31 human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu Accepted 2010-APR-24 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 31 imex curation A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. A higher-order complex containing AF4 and ENL family proteins with P-TEFb facilitates oncogenic and physiologic MLL-dependent transcription. Cancer Cell (1535-6108) 2010 imex curation Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions 31 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yokoyama A., Lin M., Naresh A., Kitabayashi I., Cleary ML. Apoptosis - Interactions involving proteins with a function related to apoptosis Anti AF4 monoclonal antibodies were X linked to G Sepharose beads. Additional detail of constructs supplied by the author. ayokoyam@ncc.go.jp, mcleary@stanford.edu Accepted 2010-APR-24 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. Cancer Cell (1535-6108) 2010 orchard Only protein-protein interactions 31 imex curation dot1l_human Histone-lysine N-methyltransferase, H3 lysine-79 specific DOT1L KIAA1814 KMT4 Histone H3-K79 methyltransferase DOT1-like protein Lysine N-methyltransferase 4 protein protein human Homo sapiens Human MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTDDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVIFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRESKSNAATPTKGPEGKVAGPADAPMDSGAEEEKAGAATVKKPSPSKARKKKLNKKGRKMAGRKRGRPKKMNTANPERKPKKNQTALDALHAQTVSQTAASSPQDAYRSPHSPFYQLPPSVQRHSPNPLLVAPTPPALQKLLESFKIQYLQFLAYTKTPQYKASLQELLGQEKEKNAQLLGAAQQLLSHCQAQKEEIRRLFQQKLDELGVKALTYNDLIQAQKEISAHNQQLREQSEQLEQDNRALRGQSLQLLKARCEELQLDWATLSLEKLLKEKQALKSQISEKQRHCLELQISIVELEKSQRQQELLQLKSCVPPDDALSLHLRGKGALGRELEPDASRLHLELDCTKFSLPHLSSMSPELSMNGQAAGYELCGVLSRPSSKQNTPQYLASPLDQEVVPCTPSHVGRPRLEKLSGLAAPDYTRLSPAKIVLRRHLSQDHTVPGRPAASELHSRAEHTKENGLPYQSPSVPGSMKLSPQDPRPLSPGALQLAGEKSSEKGLRERAYGSSGELITSLPISIPLSTVQPNKLPVSIPLASVVLPSRAERARSTPSPVLQPRDPSSTLEKQIGANAHGAGSRSLALAPAGFSYAGSVAISGALAGSPASLTPGAEPATLDESSSSGSLFATVGSRSSTPQHPLLLAQPRNSLPASPAHQLSSSPRLGGAAQGPLPEASKGDLPSDSGFSDPESEAKRRIVFTITTGAGSAKQSPSSKHSPLTASARGDCVPSHGQDSRRRGRRKRASAGTPSLSAGVSPKRRALPSVAGLFTQPSGSPLNLNSMVSNINQPLEITAISSPETSLKSSPVPYQDHDQPPVLKKERPLSQTNGAHYSPLTSDEEPGSEDEPSSARIERKIATISLESKSPPKTLENGGGLAGRKPAPAGEPVNSSKWKSTFSPISDIGLAKSADSPLQASSALSQNSLFTFRPALEEPSADAKLAAHPRKGFPGSLSGADGLSPGTNPANGCTFGGGLAADLSLHSFSDGASLPHKGPEAAGLSSPLSFPSQRGKEGSDANPFLSKRQLDGLAGLKGEGSRGKEAGEGGLPLCGPTDKTPLLSGKAAKARDREVDLKNGHNLFISAAAVPPGSLLSGPGLAPAASSAGGAASSAQTHRSFLGPFPPGPQFALGPMSLQANLGSVAGSSVLQSLFSSVPAAAGLVHVSSAATRLTNSHAMGSFSGVAGGTVGGN DIP protein Q8TEK3 original sequence version: 90 DIP protein Q8TEK3 original sequence version: 110 9CEA12850DC4ACB1 mll_aff4_fusion-1 Fusion of Human MLL (N -terminal region) and Human AFF4 ( C terminal region). protein protein human Homo sapiens Human An amino acid sequence which is not represented in UniProt q2q440_human protein protein human Homo sapiens Human FLCASSGHVEDANKESSKTS C2131CFE37A4CEAD q1wd23_human MLL/AF6 fusion protein protein human Homo sapiens Human RPSIPVKQKPKEKEKPPPVNKQENAGTLNILSTLSNGNSSKQKIPADGVHRIRVDFKDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRL B91B9C7219F768FC q03164-pro_0000390949 MLL cleavage product N320 KMT2A ALL-1 ALL1 CXXC-type zinc finger protein 7 CXXC7 HRX HTRX MLL MLL1 Myeloid/lymphoid or mixed-lineage leukemia Myeloid/lymphoid or mixed-lineage leukemia protein 1 TRX1 Trithorax-like protein Zinc finger protein HRX Cysteine methyltransferase KMT2A protein protein human Homo sapiens Human MAHSCRWRFPARPGTTGGGGGGGRRGLGGAPRQRVPALLLPPGPPVGGGGPGAPPSPPAVAAAAAAAGSSGAGVPGGAAAASAASSSSASSSSSSSSSASSGPALLRVGPGFDAALQVSAAIGTNLRRFRAVFGESGGGGGSGEDEQFLGFGSDEEVRVRSPTRSPSVKTSPRKPRGRPRSGSDRNSAILSDPSVFSPLNKSETKSGDKIKKKDSKSIEKKRGRPPTFPGVKIKITHGKDISELPKGNKEDSLKKIKRTPSATFQQATKIKKLRAGKLSPLKSKFKTGKLQIGRKGVQIVRRRGRPPSTERIKTPSGLLINSELEKPQKVRKDKEGTPPLTKEDKTVVRQSPRRIKPVRIIPSSKRTDATIAKQLLQRAKKGAQKKIEKEAAQLQGRKVKTQVKNIRQFIMPVVSAISSRIIKTPRRFIEDEDYDPPIKIARLESTPNSRFSAPSCGSSEKSSAASQHSSQMSSDSSRSSSPSVDTSTDSQASEEIQVLPEERSDTPEVHPPLPISQSPENESNDRRSRRYSVSERSFGSRTTKKLSTLQSAPQQQTSSSPPPPLLTPPPPLQPASSISDHTPWLMPPTIPLASPFLPASTAPMQGKRKSILREPTFRWTSLKHSRSEPQYFSSAKYAKEGLIRKPIFDNFRPPPLTPEDVGFASGFSASGTAASARLFSPLHSGTRFDMHKRSPLLRAPRFTPSEAHSRIFESVTLPSNRTSAGTSSSGVSNRKRKRKVFSPIRSEPRSPSHSMRTRSGRLSSSELSPLTPPSSVSSSLSISVSPLATSALNPTFTFPSHSLTQSGESAEKNQRPRKQTSAPAEPFSSSSPTPLFPWFTPGSQTERGRNKDKAPEELSKDRDADKSVEKDKSRERDREREKENKRESRKEKRKKGSEIQSSSALYPVGRVSKEKVVGEDVATSSSAKKATGRKKSSSHDSGTDITSVTLGDTTAVKTKILIKKGRGNLEKTNLDLGPTAPSLEKEKTLCLSTPSSSTVKHSTSSIGSMLAQADKLPMTDKRVASLLKKAKAQLCKIEKSKSLKQTDQPKAQGQESDSSETSVRGPRIKHVCRRAAVALGRKRAVFPDDMPTLSALPWEEREKILSSMGNDDKSSIAGSEDAEPLAPPIKPIKPVTRNKAPQEPPVKKGRRSRRCGQCPGCQVPEDCGVCTNCLDKPKFGGRNIKKQCCKMRKCQNLQWMPSKAYLQKQAKAVKKKEKKSKTSEKKDSKESSVVKNVVDSSQKPTPSAREDPAPKKSSSEPPPRKPVEEKSEEGNVSAPGPESKQATTPASRKSSKQVSQPALVIPPQPPTTGPPRKEVPKTTPSEPKKKQPPPPESGPEQSKQKKVAPRPSIPVKQKPKEKEKPPPVNKQENAGTLNILSTLSNGNSSKQKIPADGVHRIRVDFKEDCEAENVWEMGGLGILTSVPITPRVVCFLCASSGHVEFVYCQVCCEPFHKFCLEENERPLEDQLENWCCRRCKFCHVCGRQHQATKQLLECNKCRNSYHPECLGPNYPTKPTKKKKVWICTKCVRCKSCGSTTPGKGWDAQWSHDFSLCHDCAKLFAKGNFCPLCDKCYDDDDYESKMMQCGKCDRWVHSKCENLSDEMYEILSNLPESVAYTCVNCTERHPAEWRLALEKELQISLKQVLTALLNSRTTSHLLRYRQAAKPPDLNPETEESIPSRSSPEGPDPPVLTEVSKQDDQQPLDLEGVKRKMDQGNYTSVLEFSDDIVKIIQAAINSDGGQPEIKKANSMVKSFFIRQMERVFPWFSVKKSRFWEPNKVSSNSGMLPNAVLPPSLDHNYAQWQEREENSHTEQPPLMKKIIPAPKPKGPGEPDSPTPLHPPTPPILSTDRSREDSPELNPPPGIEDNRQCALCLTYGDDSANDAGRLLYIGQNEWTHVNCALWSAEVFEDDDGSLKNVHMAVIRGKQLRCEFCQKPGATVGCCLTSCTSNYHFMCSRAKNCVFLDDKKVYCQRHRDLIKGEVVPENGFEVFRRVFVDFEGISLRRKFLNGLEPENIHMMIGSMTIDCLGILNDLSDCEDKLFPIGYQCSRVYWSTTDARKRCVYTCKIVECRPPVVEPDINSTVEHDENRTIAHSPTSFTESSSKESQNTAEIISPPSPDRPPHSQTSGSCYYHVISKVPRIRTPSYSPTQRSPGCRPLPSAGSPTPTTHEIVTVGDPLLSSGLRSIGSRRHSTSSLSPQRSKLRIMSPMRTGNTYSRNNVSSVSTTGTATDLESSAKVVDHVLGPLNSSTSLGQNTSTSSNLQRTVVTVGNKNSHLDGSSSSEMKQSSASDLVSKSSSLKGEKTKVLSSKSSEGSAHNVAYPGIPKLAPQVHNTTSRELNVSKIGSFAEPSSVSFSSKEALSFPHLHLRGQRNDRDQHTDSTQSANSSPDEDTEVKTLKLSGMSNRSSIINEHMGSSSRDRRQKGKKSCKETFKEKHSSKSFLEPGQVTTGEEGNLKPEFMDEVLTPEYMGQRPCNNVSSDKIGDKGLSMPGVPKAPPMQVEGSAKELQAPRKRTVKVTLTPLKMENESQSKNALKESSPASPLQIESTSPTEPISASENPGDGPVAQPSPNNTSCQDSQSNNYQNLPVQDRNLMLPDGPKPQEDGSFKRRYPRRSARARSNMFFGLTPLYGVRSYGEEDIPFYSSSTGKKRGKRSAEGQVDGADDLSTSDEDDLYYYNFTRTVISSGGEERLASHNLFREEEQCDLPKISQLD Amino acids 1 � 2718. An N-terminal cleavage product of 320 kDa, identifier Q03164- PRO_0000390949. Short name is p320. 1 2718 08B1A59A345C4CFD aff1_human AF4/FMR2 family member 1 AFF1 AF4 FEL MLLT2 PBM1 ALL1-fused gene from chromosome 4 protein Proto-oncogene AF4 Protein FEL protein protein human Homo sapiens Human MAAQSSLYNDDRNLLRIREKERRNQEAHQEKEAFPEKIPLFGEPYKTAKGDELSSRIQNMLGNYEEVKEFLSTKSHTHRLDASENRLGKPKYPLIPDKGSSIPSSSFHTSVHHQSIHTPASGPLSVGNISHNPKMAQPRTEPMPSLHAKSCGPPDSQHLTQDRLGQEGFGSSHHKKGDRRADGDHCASVTDSAPERELSPLISLPSPVPPLSPIHSNQQTLPRTQGSSKVHGSSNNSKGYCPAKSPKDLAVKVHDKETPQDSLVAPAQPPSQTFPPPSLPSKSVAMQQKPTAYVRPMDGQDQAPSESPELKPLPEDYRQQTFEKTDLKVPAKAKLTKLKMPSQSVEQTYSNEVHCVEEILKEMTHSWPPPLTAIHTPSTAEPSKFPFPTKDSQHVSSVTQNQKQYDTSSKTHSNSQQGTSSMLEDDLQLSDSEDSDSEQTPEKPPSSSAPPSAPQSLPEPVASAHSSSAESESTSDSDSSSDSESESSSSDSEENEPLETPAPEPEPPTTNKWQLDNWLTKVSQPAAPPEGPRSTEPPRRHPESKGSSDSATSQEHSESKDPPPKSSSKAPRAPPEAPHPGKRSCQKSPAQQEPPQRQTVGTKQPKKPVKASARAGSRTSLQGEREPGLLPYGSRDQTSKDKPKVKTKGRPRAAASNEPKPAVPPSSEKKKHKSSLPAPSKALSGPEPAKDNVEDRTPEHFALVPLTESQGPPHSGSGSRTSGCRQAVVVQEDSRKDRLPLPLRDTKLLSPLRDTPPPQSLMVKITLDLLSRIPQPPGKGSRQRKAEDKQPPAGKKHSSEKRSSDSSSKLAKKRKGEAERDCDNKKIRLEKEIKSQSSSSSSSHKESSKTKPSRPSSQSSKKEMLPPPPVSSSSQKPAKPALKRSRREADTCGQDPPKSASSTKSNHKDSSIPKQRRVEGKGSRSSSEHKGSSGDTANPFPVPSLPNGNSKPGKPQVKFDKQQADLHMREAKKMKQKAELMTDRVGKAFKYLEAVLSFIECGIATESESQSSKSAYSVYSETVDLIKFIMSLKSFSDATAPTQEKIFAVLCMRCQSILNMAMFRCKKDIAIKYSRTLNKHFESSSKVAQAPSPCIASTGTPSPLSPMPSPASSVGSQSSAGSVGSSGVAATISTPVTIQNMTSSYVTITSHVLTAFDLWEQAEALTRKNKEFFARLSTNVCTLALNSSLVDLVHYTRQGFQQLQELTKTP DIP protein P51825 original sequence version: 102 F0E334DF8FC2FF04 q8td98_human protein protein human Homo sapiens Human TTGPPRKEVPKTTPSEPKKKQPPPPESGPEQSKQKKVAPRPSIPVKQKPKEKEKPPPVNKQENAGTLNILSTLSNGNSSKQKIPADGVHRIRVDFKEDCEAENVWEMGGLGILTSVPITPRVVCFLCASSGHVEQTYSNEVHCVEEILKEMTHSWPPPLAAIHTPSTAEPSKFPFPTKDSQHVSSVTQNQKQYDTSSKTHSNSQQGTSSMLEDDLQLSDSEDSDSEQTPEKPPSHLHLQVLHSPFQNQWHQHIPAVQSQKAPVTQTVPQTQRARAVQVTASSSDSEENEPLETPAPEPEPPTTNKWQLDNWLTKVSQPAAPPEGPRSTEPPRRHPESKGSSDSATSQEHSESKDPPPKSSSKAPRAPPEAPHPGKRSCQKSPAQQEPPQRQTVGTKQPKKPVKASARAGSRTSLQGEREPGLLPYGSRDQTSKDKPKVKTKGRPRAAASNEPKPAVPPSSEKKKHKSSLPAPSKALSGPEPAKDNVEDRTPEHFALVPLTESQGPPHSGSGSRTSGCRQAVVVQEDSRKDRLPLPLRDTKLLSPLRDTPPPQSLMVKITLDLLSRIPQPPGKGSRQRKAEDKQPPAGKKHSSEKRSSDSSSKLAKKRKGEAERDCDNKKIRLEK 712B9258F5E272AD mll_enl_human_protein Fusion of human MLL (N-terminal region) with human ENL C-terminal residues 373-559 protein protein human Homo sapiens Human An amino acid sequence which is not represented in UniProt enl_human Protein ENL MLLT1 ENL LTG19 YEATS1 YEATS domain-containing protein 1 protein protein human Homo sapiens Human MDNQCTVQVRLELGHRAQLRKKPTTEGFTHDWMVFVRGPEQCDIQHFVEKVVFWLHDSFPKPRRVCKEPPYKVEESGYAGFIMPIEVHFKNKEEPRKVCFTYDLFLNLEGNPPVNHLRCEKLTFNNPTTEFRYKLLRAGGVMVMPEGADTVSRPSPDYPMLPTIPLSAFSDPKKTKPSHGSKDANKESSKTSKPHKVTKEHRERPRKDSESKSSSKELEREQAKSSKDTSRKLGEGRLPKEEKAPPPKAAFKEPKMALKETKLESTSPKGGPPPPPPPPPRASSKRPATADSPKPSAKKQKKSSSKGSRSAPGTSPRTSSSSSFSDKKPAKDKSSTRGEKVKAESEPREAKKALEVEESNSEDEASFKSESAQSSPSNSSSSSDSSSDSDFEPSQNHSQGPLRSMVEDLQSEESDEDDSSSGEEAAGKTNPGRDSRLSFSDSESDNSADSSLPSREPPPPQKPPPPNSKVSGRRSPESCSKPEKILKKGTYDKAYTDELVELHRRLMALRERNVLQQIVNLIEETGHFNVTNTTFDFDLFSLDETTVRKLQSCLEAVAT A8480BA0F8742173 aff4_human AF4/FMR2 family member 4 AFF4 AF5Q31 MCEF ALL1-fused gene from chromosome 5q31 protein Major CDK9 elongation factor-associated protein HSPC092 protein protein human Homo sapiens Human MNREDRNVLRMKERERRNQEIQQGEDAFPPSSPLFAEPYKVTSKEDKLSSRIQSMLGNYDEMKDFIGDRSIPKLVAIPKPTVPPSADEKSNPNFFEQRHGGSHQSSKWTPVGPAPSTSQSQKRSSGLQSGHSSQRTSAGSSSGTNSSGQRHDRESYNNSGSSSRKKGQHGSEHSKSRSSSPGKPQAVSSLNSSHSRSHGNDHHSKEHQRSKSPRDPDANWDSPSRVPFSSGQHSTQSFPPSLMSKSNSMLQKPTAYVRPMDGQESMEPKLSSEHYSSQSHGNSMTELKPSSKAHLTKLKIPSQPLDASASGDVSCVDEILKEMTHSWPPPLTAIHTPCKTEPSKFPFPTKESQQSNFGTGEQKRYNPSKTSNGHQSKSMLKDDLKLSSSEDSDGEQDCDKTMPRSTPGSNSEPSHHNSEGADNSRDDSSSHSGSESSSGSDSESESSSSDSEANEPSQSASPEPEPPPTNKWQLDNWLNKVNPHKVSPASSVDSNIPSSQGYKKEGREQGTGNSYTDTSGPKETSSATPGRDSKTIQKGSESGRGRQKSPAQSDSTTQRRTVGKKQPKKAEKAAAEEPRGGLKIESETPVDLASSMPSSRHKAATKGSRKPNIKKESKSSPRPTAEKKKYKSTSKSSQKSREIIETDTSSSDSDESESLPPSSQTPKYPESNRTPVKPSSVEEEDSFFRQRMFSPMEEKELLSPLSEPDDRYPLIVKIDLNLLTRIPGKPYKETEPPKGEKKNVPEKHTREAQKQASEKVSNKGKRKHKNEDDNRASESKKPKTEDKNSAGHKPSSNRESSKQSAAKEKDLLPSPAGPVPSKDPKTEHGSRKRTISQSSSLKSSSNSNKETSGSSKNSSSTSKQKKTEGKTSSSSKEVKEKAPSSSSNCPPSAPTLDSSKPRRTKLVFDDRNYSADHYLQEAKKLKHNADALSDRFEKAVYYLDAVVSFIECGNALEKNAQESKSPFPMYSETVDLIKYTMKLKNYLAPDATAADKRLTVLCLRCESLLYLRLFKLKKENALKYSKTLTEHLKNSYNNSQAPSPGLGSKAVGMPSPVSPKLSPGNSGNYSSGASSASASGSSVTIPQKIHQMAASYVQVTSNFLYATEIWDQAEQLSKEQKEFFAELDKVMGPLIFNASIMTDLVRYTRQGLHWLRQDAKLIS 474E1906B8832AC1 men1_human Menin MEN1 SCG2 protein protein human Homo sapiens Human MGLKAAQKTLFPLRSIDDVVRLFAAELGREEPDLVLLSLVLGFVEHFLAVNRVIPTNVPELTFQPSPAPDPPGGLTYFPVADLSIIAALYARFTAQIRGAVDLSLYPREGGVSSRELVKKVSDVIWNSLSRSYFKDRAHIQSLFSFITGWSPVGTKLDSSGVAFAVVGACQALGLRDVHLALSEDHAWVVFGPNGEQTAEVTWHGKGNEDRRGQTVNAGVAERSWLYLKGSYMRCDRKMEVAFMVCAINPSIDLHTDSLELLQLQQKLLWLLYDLGHLERYPMALGNLADLEELEPTPGRPDPLTLYHKGIASAKTYYRDEHIYPYMYLAGYHCRNRNVREALQAWADTATVIQDYNYCREDEEIYKEFFEVANDVIPNLLKEAASLLEAGEERPGEQSQGTQSQGSALQDPECFAHLLRFYDGICKWEEGSPTPVLHVGWATFLVQSLGRFEGQVRQKVRIVSREAEAAEAEEPWGEEAREGRRRGPRRESKPEEPPPPKKPALDKGLGTGQGAVSGPPRKPPGTVAGTARGPEGGSTAQVPAPTASPPPEGPVLTFQSEKMKGMKELLVATKINSSAIKLQLTAQSQVQMKKQKVSTPSDYTLSFLKRQRKGL DIP protein O00255 original sequence version: 100 DIP protein O00255 original sequence version: 131 DDDF850EA5AB77B4 af9_human Protein AF-9 ALL1-fused gene from chromosome 9 protein Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein YEATS domain-containing protein 3 MLLT3 AF9 YEATS3 protein protein human Homo sapiens Human MASSCAVQVKLELGHRAQVRKKPTVEGFTHDWMVFVRGPEHSNIQHFVEKVVFHLHESFPRPKRVCKDPPYKVEESGYAGFILPIEVYFKNKEEPRKVRFDYDLFLHLEGHPPVNHLRCEKLTFNNPTEDFRRKLLKAGGDPNRSIHTSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSSTSFSKPHKLMKEHKEKPSKDSREHKSAFKEPSRDHNKSSKESSKKPKENKPLKEEKIVPKMAFKEPKPMSKEPKPDSNLLTITSGQDKKAPSKRPPISDSEELSAKKRKKSSSEALFKSFSSAPPLILTCSADKKQIKDKSHVKMGKVKIESETSEKKKSTLPPFDDIVDPNDSDVEENISSKSDSEQPSPASSSSSSSSSFTPSQTRQQGPLRSIMKDLHSDDNEEESDEVEDNDNDSEMERPVNRGGSRSRRVSLSDGSDSESSSASSPLHHEPPPPLLKTNNNQILEVKSPIKQSKSDKQIKNGECDKAYLDELVELHRRLMTLRERHILQQIVNLIEETGHFHITNTTFDFDLCSLDKTTVRKLQSYLETSGTS DIP protein P42568 original sequence version: 135 DIP protein P42568 original sequence version: 103 DIP protein P42568 original sequence version: 136 0A020B7FB34132F9 ccnt1_human Cyclin-T1 CCNT1 protein protein human Homo sapiens Human MEGERKNNNKRWYFTREQLENSPSRRFGVDPDKELSYRQQAANLLQDMGQRLNVSQLTINTAIVYMHRFYMIQSFTQFPGNSVAPAALFLAAKVEEQPKKLEHVIKVAHTCLHPQESLPDTRSEAYLQQVQDLVILESIILQTLGFELTIDHPHTHVVKCTQLVRASKDLAQTSYFMATNSLHLTTFSLQYTPPVVACVCIHLACKWSNWEIPVSTDGKHWWEYVDATVTLELLDELTHEFLQILEKTPNRLKRIWNWRACEAAKKTKADDRGTDEKTSEQTILNMISQSSSDTTIAGLMSMSTSTTSAVPSLPVSEESSSNLTSVEMLPGKRWLSSQPSFKLEPTQGHRTSENLALTGVDHSLPQDGSNAFISQKQNSKSVPSAKVSLKEYRAKHAEELAAQKRQLENMEANVKSQYAYAAQNLLSHHDSHSSVILKMPIEGSENPERPFLEKADKTALKMRIPVAGGDKAASSKPEEIKMRIKVHAAADKHNSVEDSVTKSREHKEKHKTHPSNHHHHHNHHSHKHSHSQLPVGTGNKRPGDPKHSSQTSNLAHKTYSLSSSFSSSSSTRKRGPSEETGGAVFDHPAKIAKSTKSSSLNFSFPSLPTMGQMPGHSSDTSGLSFSQPSCKTRVPHSKLDKGPTGANGHNTTQTIDYQDTVNMLHSLLSAQGVQPTQPTAFEFVRPYSDYLNPRSGGISSRSGNTDKPRPPPLPSEPPPPLPPLPK DIP protein O60563 original sequence version: 85 DIP protein O60563 original sequence version: 79 DIP protein O60563 original sequence version: 98 DIP protein O60563 original sequence version: 102 DIP protein O60563 original sequence version: 164 DIP protein O60563 original sequence version: 125 DIP protein O60563 original sequence version: 103 4637EFB2DDEDFE13 q03164-pro_0000390950 MLL cleavage product C180 KMT2A ALL-1 ALL1 CXXC-type zinc finger protein 7 CXXC7 HRX HTRX MLL MLL1 Myeloid/lymphoid or mixed-lineage leukemia Myeloid/lymphoid or mixed-lineage leukemia protein 1 TRX1 Trithorax-like protein Zinc finger protein HRX Cysteine methyltransferase KMT2A protein protein human Homo sapiens Human GVDDGTESDTSVTATTRKSSQIPKRNGKENGTENLKIDRPEDAGEKEHVTKSSVGHKNEPKMDNCHSVSRVKTQGQDSLEAQLSSLESSRRVHTSTPSDKNLLDTYNTELLKSDSDNNNSDDCGNILPSDIMDFVLKNTPSMQALGESPESSSSELLNLGEGLGLDSNREKDMGLFEVFSQQLPTTEPVDSSVSSSISAEEQFELPLELPSDLSVLTTRSPTVPSQNPSRLAVISDSGEKRVTITEKSVASSESDPALLSPGVDPTPEGHMTPDHFIQGHMDADHISSPPCGSVEQGHGNNQDLTRNSSTPGLQVPVSPTVPIQNQKYVPNSTDSPGPSQISNAAVQTTPPHLKPATEKLIVVNQNMQPLYVLQTLPNGVTQKIQLTSSVSSTPSVMETNTSVLGPMGGGLTLTTGLNPSLPTSQSLFPSASKGLLPMSHHQHLHSFPAATQSSFPPNISNPPSGLLIGVQPPPDPQLLVSESSQRTDLSTTVATPSSGLKKRPISRLQTRKNKKLAPSSTPSNIAPSDVVSNMTLINFTPSQLPNHPSLLDLGSLNTSSHRTVPNIIKRSKSSIMYFEPAPLLPQSVGGTAATAAGTSTISQDTSHLTSGSVSGLASSSSVLNVVSMQTTTTPTSSASVPGHVTLTNPRLLGTPDIGSISNLLIKASQQSLGIQDQPVALPPSSGMFPQLGTSQTPSTAAITAASSICVLPSTQTTGITAASPSGEADEHYQLQHVNQLLASKTGIHSSQRDLDSASGPQVSNFTQTVDAPNSMGLEQNKALSSAVQASPTSPGGSPSSPSSGQRSASPSVPGPTKPKPKTKRFQLPLDKGNGKKHKVSHLRTSSSEAHIPDQETTSLTSGTGTPGAEAEQQDTASVEQSSQKECGQPAGQVAVLPEVQVTQNPANEQESAEPKTVEEEESNFSSPLMLWLQQEQKRKESITEKKPKKGLVFEISSDDGFQICAESIEDAWKSLTDKVQEARSNARLKQLSFAGVNGLRMLGILHDAVVFLIEQLSGAKHCRNYKFRFHKPEEANEPPLNPHGSARAEVHLRKSAFDMFNFLASKHRQPPEYNPNDEEEEEVQLKSARRATSMDLPMPMRFRHLKKTSKEAVGVYRSPIHGRGLFCKRNIDAGEMVIEYAGNVIRSIQTDKREKYYDSKGIGCYMFRIDDSEVVDATMHGNAARFINHSCEPNCYSRVINIDGQKHIVIFAMRKIYRGEELTYDYKFPIEDASNKLPCNCGAKKCRKFLN Amino acids 2719 � 3969. An C-terminal cleavage product of 180 kDa, identifier Q03164-PRO_0000390950. Short name is p180. 2719 3969 C15A37D79569D083 cdk9_human Cyclin-dependent kinase 9 CDK9 CDC2L4 Cell division protein kinase 9 Serine/threonine-protein kinase PITALRE Cell division cycle 2-like protein kinase 4 C-2K TAK Tat-associated kinase complex catalytic subunit protein protein human Homo sapiens Human MAKQYDSVECPFCDEVSKYEKLAKIGQGTFGEVFKARHRKTGQKVALKKVLMENEKEGFPITALREIKILQLLKHENVVNLIEICRTKASPYNRCKGSIYLVFDFCEHDLAGLLSNVLVKFTLSEIKRVMQMLLNGLYYIHRNKILHRDMKAANVLITRDGVLKLADFGLARAFSLAKNSQPNRYTNRVVTLWYRPPELLLGERDYGPPIDLWGAGCIMAEMWTRSPIMQGNTEQHQLALISQLCGSITPEVWPNVDNYELYEKLELVKGQKRKVKDRLKAYVRDPYALDLIDKLLVLDPAQRIDSDDALNHDFFWSDPMPSDLKGMLSTHLTSMFEYLAPPRRKGSQITQQSTNQSRNPATTNQTEFERVF DIP protein P50750 original sequence version: 100 DIP protein P50750 original sequence version: 115 DIP protein P50750-1 original sequence version: 119 DIP protein P50750 original sequence version: 170 DIP protein P50750 original sequence version: 184 DIP protein P50750-1 original sequence version: 144 DIP protein P50750 original sequence version: 70 DIP protein P50750 original sequence version: 120 69E851CC6F7A0388 mll_aff4_fusion Fusion of Human MLL (N -terminus) and Human AFF4 (518-1163) -C terminal region. protein protein human Homo sapiens Human An amino acid sequence which is not represented in UniProt af4-aff1-1 5 24 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait 19 unspecified role unspecified role prey prey association association Fig 1B. In the identification of AF4-associated proteins in vivo, Mass spectrometry identified AFF4/AF5q31, ENL, CDK9, and cyclin T1 in the purified materials. men1-mlln 8 20 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait physical association physical association Fig 1C. MLL did not pull down AEP components in K562 cells while coprecipitating menin, a component of the MLL complex. af4-2 6 19 unspecified role unspecified role bait bait 18 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey association association Fig S1A. I am assuming "AF5" on the image is the same as AF5q31 - as the only entry for AF5 in SwissP gives MLL1 - which looks to be wrong, and no entries seen anywhere else (in the paper, supplement, google) for "AF5". AFF1/AF4, AFF4/AF5q31, and ENL associate in an endogenous higher-order complex. af4-3 8 19 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait association association Fig 1C. Reciprocal immunoprecipitation further confirmed that all five proteins compose an endogenous complex consistent with previous observations. af4-4 8 24 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait 18 unspecified role unspecified role prey prey association association Fig 1C. Reciprocal immunoprecipitation further confirmed that all five proteins compose an endogenous complex consistent with previous observations. af4-5 8 18 unspecified role unspecified role bait bait 19 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey association association Fig 1C. Reciprocal immunoprecipitation further confirmed that all five proteins compose an endogenous complex consistent with previous observations. mlln-mllc 8 23 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait physical association physical association Fig 1C. Reciprocal immunoprecipitation further confirmed that all five proteins compose an endogenous complex consistent with previous observations. ?this could have been amalgamated with EBI-2610215 -(same bait) but as they were done in 2 different expts I have left them separate. af4-7 8 24 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey association association Fig 1C. Reciprocal immunoprecipitation further confirmed that all five proteins compose an endogenous complex consistent with previous observations. af4-8 1 14 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey association association Fig 1E. Reciprocal IP using human leukemia cell lines that express MLL-ENL, MLL-AF4, or MLL-AF5q31 showed that the respective fusion proteins form similar AEP-like complexes. af4-9 1 18 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait 22 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey association association Fig 1E. Reciprocal IP using human leukemia cell lines that express MLL-ENL, MLL-AF4, or MLL-AF5q31 showed that the respective fusion proteins form similar AEP-like complexes. af4-10 2 16 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait 18 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 15 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey association association Fig 1E. Reciprocal IP using human leukemia cell lines that express MLL-ENL, MLL-AF4, or MLL-AF5q31 showed that the respective fusion proteins form similar AEP-like complexes. I see no band for AFF4/AF5q31 (nor did Marine) af4-11 2 16 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait 22 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey association association Fig 1E. Reciprocal IP using human leukemia cell lines that express MLL-ENL, MLL-AF4, or MLL-AF5q31 showed that the respective fusion proteins form similar AEP-like complexes. mlln-mlaf6 3 13 unspecified role unspecified role prey prey 14 unspecified role unspecified role bait bait physical association physical association Fig 1E. MLL-AF6, an MLL fusion with a cytoplasmic protein that was not copurified with AF4 or AF5q31, did not coprecipitate any of the AEP components in ML-2 cells. ML-2 cells have no normal MLL gene. af4-13 3 19 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait 22 unspecified role unspecified role prey prey association association Fig 1E. MLL-AF6, an MLL fusion with a cytoplasmic protein that was not copurified with AF4 or AF5q31, did not coprecipitate any of the AEP components in ML-2 cells. ML-2 cells have no normal MLL gene. aff1-ptefb 4 24 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait gal4bd GAL4 DNA binding domain fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined subregion_1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain association association Fig 3B. P-TEFb interacts with AFF1/AF4 and AFF4/AF5q31 via subregion 1, which contains the N-terminal homology domain (NHD). aff4-ptefb 4 24 unspecified role unspecified role prey prey 19 unspecified role unspecified role bait bait subregion_1 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined gal4bd GAL4 DNA binding domain fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role prey prey association association Fig 3B. P-TEFb interacts with AFF1/AF4 and AFF4/AF5q31 via subregion 1, which contains the N-terminal homology domain (NHD). I have not added the NHD as a feature - as it is not listed in SwissP/InterPro and it is much smaller than this "subregion". I cannot see that ENL is producing a band here - but it is hard to see anything - so not entered. FLAG tag is attached to GAL4 fusion proteins according to author. enl-aff4 4 18 unspecified role unspecified role bait bait Q03111:p.Leu550Glu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain L E Wed May 02 17:16:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu550glu' Tue Jun 30 19:05:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu550Glu' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined gal4bd GAL4 DNA binding domain fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 19 unspecified role unspecified role prey prey physical association physical association Fig 4B. C-terminal ENL residues (494�559) are required for the interaction with (endogenous) AFF4/AF5q31. af9-dot1l 9 10 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 21 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P42568:p.Leu558Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L E Wed May 02 18:10:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu558glu' Tue Jun 30 18:59:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu558Glu' c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gal4bd GAL4 DNA binding domain fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 4D. The AHD of ENL also mediated association with DOT1L. This (AHD) region is evolutionally conserved in AF9. lys558glu entered as "mutation decreasing" as the mutation is not specifically mentioned in the text at all- though the band has vanished. enl-dot1l 9 10 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 18 unspecified role unspecified role bait bait Q03111:p.Leu550Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L E Wed May 02 17:30:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu550glu' Tue Jun 30 18:54:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu550Glu' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined gal4bd GAL4 DNA binding domain fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 4D. The AHD of ENL also mediated association with DOT1L. af9-aff4 4 19 unspecified role unspecified role prey prey 21 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P42568:p.Leu558Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain L E Wed May 02 17:25:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu558glu' Tue Jun 30 15:37:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu558Glu' c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain gal4bd GAL4 DNA binding domain fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 4B. C-terminal ENL residues (494�559) are required for the interaction with (endogenous) AFF4/AF5q31. This (AHD) region is evolutionally conserved in AF9. lys558glu entered as "mutation decreasing" as the mutation is not specifically mentioned in the text at all- though the band has vanished. dot1l-enl 9 18 unspecified role unspecified role prey prey xpress-tag Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys Tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 5A. Diificult to tally lanes with "headings" for this blot. Not sure if I have entered this "rare" tag as I should. ENL coprecipitated both AFF4/AF5q31 and DOT1L, but the latter two did not pull down each other, indicating that the three proteins do not form a trimeric complex. enl-dot1l-1 9 19 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 18 unspecified role unspecified role bait bait xpress-tag Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys Tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig 5A. Diificult to tally lanes with "headings" for this blot. Not sure if I have entered the "rare" Xpress tag as I should. ENL coprecipitated both AFF4/AF5q31 and DOT1L, but the latter two did not pull down each other, indicating that the three proteins do not form a trimeric complex. aff4-enl 9 18 unspecified role unspecified role prey prey xpress-tag Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys Tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 19 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 5A. Diificult to tally lanes with "headings" for this blot. Not sure if I have entered the "rare" Xpress tag as I should. ENL coprecipitated both AFF4/AF5q31 and DOT1L, but the latter two did not pull down each other, indicating that the three proteins do not form a trimeric complex. Dot1l and AF4 family proteins mutually exclusively bind to the same region of ENL. mll-enl-aff1 9 17 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig 5C. FLAG-tagged MLL fusion proteins were coexpressed with AFF1 or DOT1L. mll-aff4-aff1 9 11 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig 5C. Artificial MLL fusion protein (with AFF4) that does associate with AFF1. aff1-ptefb-1 7 22 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait 19 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey association association Fig S1B. Reciprocal IP using human leukemia cell lines that express MLL-ENL, MLL-AFF1(AF4), or MLL-AFF1(AF5q31) showed that the respective fusion proteins form similar AEP-like complexes. mll-ptefb 7 14 unspecified role unspecified role bait bait 19 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey association association Fig S1B. Reciprocal IP using human leukemia cell lines that express MLL-ENL, MLL-AFF1(AF4), or MLL-AFF1(AF5q31) showed that the respective fusion proteins form similar AEP-like complexes. mll-aff4-34region 9 25 unspecified role unspecified role prey prey xpress-tag Asp-Leu-Tyr-Asp-Asp-Asp-Asp-Lys Tag fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role prey prey 15 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig S3A. Preferential hetero-dimerization was also observed in co-IP experiments of endogenous or transfected MLL-AFF4(AF5q31). I dont see how the sequence 518-1163 can be shown to be associated with AFF4 rather than MLL. Unless I add as a entry "create an entry using the protein editor" as a fusion protein -Section 8. So I have done this: I have created a DUPLICATE entry - I will remove whichever you dont like. Problem - I used your entry for "vamp7_hrb_fusion" to make this entry - one of the Xrefs is "see-also" - I have not used this as I cannot make out from the Manual if I should have done...( I could have added the Xpress tag to the "mll_aff4_fusion" entry as well? - rather than add as a Feature?) aff4-ct1 9 19 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role prey prey physical association physical association Fig S3A. Preferential hetero-dimerization was also observed in co-IP experiments of endogenous or transfected MLL-AFF4(AF5q31). enl-dot1l-2 9 10 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 18 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined gal4bd GAL4 DNA binding domain fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig S4. AFF4 co-precipitates ENL but not DOT1L under conditions where ENL pulls down DOT1L. Dot1l and AF4 family proteins mutually exclusively bind to the same region of ENL. aff4-enl-1 9 18 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined gal4bd GAL4 DNA binding domain fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 19 unspecified role unspecified role bait bait subregion_3 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined gal4bd GAL4 DNA binding domain fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig S4. AFF4 co-precipitates ENL but not DOT1L under conditions where ENL pulls down DOT1L. Constructs are all FLAG tagged; and the band present in every lane at 55kDa is human IgG so can be ignored...