IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The mitochondrial serine protease HtrA2/Omi cleaves RIP1 during apoptosis of Ba/F3 cells induced by growth factor withdrawal. The mitochondrial serine protease HtrA2/Omi cleaves RIP1 during apoptosis of Ba/F3 cells induced by growth factor withdrawal. Cell Res. (1001-0602) 2010 imex curation Vande Walle L., Wirawan E., Lamkanfi M., Festjens N., Verspurten J., Saelens X., Vanden Berghe T., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis peter.vandenabeele@dmbr.VIB-UGent.be 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protease assay protease assay western blot western blot Immuno blot Immuno blot Vande Walle L., Wirawan E., Lamkanfi M., Festjens N., Verspurten J., Saelens X., Vanden Berghe T., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis peter.vandenabeele@dmbr.VIB-UGent.be Accepted 2011-OCT-26 by ORCHARD Cell Res. (1001-0602) 2010 Only protein-protein interactions imex curation The mitochondrial serine protease HtrA2/Omi cleaves RIP1 during apoptosis of Ba/F3 cells induced by growth factor withdrawal. The mitochondrial serine protease HtrA2/Omi cleaves RIP1 during apoptosis of Ba/F3 cells induced by growth factor withdrawal. Cell Res. (1001-0602) 2010 imex curation Vande Walle L., Wirawan E., Lamkanfi M., Festjens N., Verspurten J., Saelens X., Vanden Berghe T., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis peter.vandenabeele@dmbr.VIB-UGent.be 21-Dec-2011: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protease assay protease assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Vande Walle L., Wirawan E., Lamkanfi M., Festjens N., Verspurten J., Saelens X., Vanden Berghe T., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis peter.vandenabeele@dmbr.VIB-UGent.be Accepted 2011-OCT-26 by ORCHARD 21-Dec-2011: Contacted by JYOTI. Cell Res. (1001-0602) 2010 Only protein-protein interactions imex curation ripk1_mouse Receptor-interacting serine/threonine-protein kinase 1 Cell death protein RIP Receptor-interacting protein 1 Ripk1 Rinp Rip protein protein mouse Mus musculus Mouse MQPDMSLDNIKMASSDLLEKTDLDSGGFGKVSLCYHRSHGFVILKKVYTGPNRAEYNEVLLEEGKMMHRLRHSRVVKLLGIIIEEGNYSLVMEYMEKGNLMHVLKTQIDVPLSLKGRIIVEAIEGMCYLHDKGVIHKDLKPENILVDRDFHIKIADLGVASFKTWSKLTKEKDNKQKEVSSTTKKNNGGTLYYMAPEHLNDINAKPTEKSDVYSFGIVLWAIFAKKEPYENVICTEQFVICIKSGNRPNVEEILEYCPREIISLMERCWQAIPEDRPTFLGIEEEFRPFYLSHFEEYVEEDVASLKKEYPDQSPVLQRMFSLQHDCVPLPPSRSNSEQPGSLHSSQGLQMGPVEESWFSSSPEYPQDENDRSVQAKLQEEASYHAFGIFAEKQTKPQPRQNEAYNREEERKRRVSHDPFAQQRARENIKSAGARGHSDPSTTSRGIAVQQLSWPATQTVWNNGLYNQHGFGTTGTGVWYPPNLSQMYSTYKTPVPETNIPGSTPTMPYFSGPVADDLIKYTIFNSSGIQIGNHNYMDVGLNSQPPNNTCKEESTSRHQAIFDNTTSLTDEHLNPIRENLGRQWKNCARKLGFTESQIDEIDHDYERDGLKEKVYQMLQKWLMREGTKGATVGKLAQALHQCCRIDLLNHLIRASQS DIP protein Q60855 original sequence version: 85 DIP protein Q60855 original sequence version: 109 DIP protein Q60855 original sequence version: 152 DIP protein Q60855 original sequence version: 130 DIP protein Q60855 original sequence version: 84 DIP protein Q60855 original sequence version: 123 DIP protein Q60855 original sequence version: 158 ABB350B523879933 htra2_human Serine protease HTRA2, mitochondrial High temperature requirement protein A2 Omi stress-regulated endoprotease Serine proteinase OMI Serine protease 25 HTRA2 PRSS25 OMI protein protein human Homo sapiens Human MAAPRAGRGAGWSLRAWRALGGIRWGRRPRLTPDLRALLTSGTSDPRARVTYGTPSLWARLSVGVTEPRACLTSGTPGPRAQLTAVTPDTRTREASENSGTRSRAWLAVALGAGGAVLLLLWGGGRGPPAVLAAVPSPPPASPRSQYNFIADVVEKTAPAVVYIEILDRHPFLGREVPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQTNVEYIQTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNSSSGISGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTPEVTE CEA955A7D0DD8C0D htra2-ripk1 1 3 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 4 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O43464:p.Ser306Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 20:24:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser306ala' Tue Jun 30 20:16:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser306Ala' This mutation disrupts the protease activity of htra2. It could still be interacting with ripk1, but the protease assay performed cannot really discern that. ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 3e htra2-ripk1-1 2 3 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined intermediate domain sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain htra2 cleavage site required to bind necessary binding region required to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Residues 451 to 456 were mutated to ala in order to determine where the cleavage site for HtrA2 was in the Ripk1 sequence. The protease assay performed doesn't allow to determine if the proteins are not interactign anymore, it just shows that the cleavage doesn't take place. rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 4 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 4a, 4b, 4c, 4d and 4f