IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Phylogenetic Profiles Reveal Structural and Functional Determinants of Lipid-binding Phylogenetic Profiles Reveal Structural and Functional Determinants of Lipid-binding J Proteomics Bioinform 2009 imex curation Hong Y., Chalkia D., Ko K.D., Bhardwaj G., Chang G.S., van Rossum D.B., Patterson R.L. Apoptosis - Interactions involving proteins with a function related to apoptosis 2009-03-25: van Rossum DB, Department of Biology, Huck Institutes of the Life Sciences, Center for Computational Proteomics, The Pennsylvania State University, PA Authors performed PIP-strip lipid binding assays using bacterially purified GST-tagged peptides of the SH2 domain from mouse BTK. dbv10@psu.edu,rlp25@psu.edu 23-Feb-2010: Contacted by JYOTI. Only protein-protein interactions imex curation in vitro In vitro affinity techniques affinity technology affinity techniques western blot western blot Immuno blot Immuno blot Hong Y., Chalkia D., Ko K.D., Bhardwaj G., Chang G.S., van Rossum D.B., Patterson R.L. Apoptosis - Interactions involving proteins with a function related to apoptosis 2009-03-25: van Rossum DB, Department of Biology, Huck Institutes of the Life Sciences, Center for Computational Proteomics, The Pennsylvania State University, PA waiting to obtain coordinates for TXK and ITK peptides Authors performed PIP-strip lipid binding assays using bacterially purified GST-tagged peptides of the SH2 domain from mouse BTK. dbv10@psu.edu,rlp25@psu.edu 23-Feb-2010: Contacted by JYOTI. J Proteomics Bioinform 2009 imex curation Phylogenetic Profiles Reveal Structural and Functional Determinants of Lipid-binding Phylogenetic Profiles Reveal Structural and Functional Determinants of Lipid-binding J Proteomics Bioinform 2009 imex curation Hong Y., Chalkia D., Ko K.D., Bhardwaj G., Chang G.S., van Rossum D.B., Patterson R.L. Apoptosis - Interactions involving proteins with a function related to apoptosis 2009-03-25: van Rossum DB, Department of Biology, Huck Institutes of the Life Sciences, Center for Computational Proteomics, The Pennsylvania State University, PA Authors performed PIP-strip lipid binding assays using bacterially purified GST-tagged peptides of the SH2 domain from mouse BTK. dbv10@psu.edu,rlp25@psu.edu 23-Feb-2010: Contacted by JYOTI. Only protein-protein interactions imex curation human-ws Homo sapiens Werner Syndrom (WS) cell line transformed with SV40 (AG11395 cell line) ws Human Werner Syndrome (WS) cell line transformed with SV40 (AG11395 cell line) affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom peptide synthesis peptide synthesis Hong Y., Chalkia D., Ko K.D., Bhardwaj G., Chang G.S., van Rossum D.B., Patterson R.L. Apoptosis - Interactions involving proteins with a function related to apoptosis 2009-03-25: van Rossum DB, Department of Biology, Huck Institutes of the Life Sciences, Center for Computational Proteomics, The Pennsylvania State University, PA Authors performed PIP-strip lipid binding assays using bacterially purified GST-tagged peptides of the SH2 domain from mouse BTK. dbv10@psu.edu,rlp25@psu.edu 23-Feb-2010: Contacted by JYOTI. J Proteomics Bioinform 2009 imex curation pi3p 1-phosphatidyl-1D-myo-inositol 3-phosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 1-phosphatidyl-1d-myo-inositol 3,5-bisphosphate 1-phosphatidyl-1D-myo-inositol 3,5-bisphosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 1-phosphatidyl-1d-myo-inositol 4,5-bisphosphate 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) btk_mouse Tyrosine-protein kinase BTK Bruton tyrosine kinase B-cell progenitor kinase Kinase EMB Btk Bpk Agammaglobulinemia tyrosine kinase protein protein mouse Mus musculus Mouse MAAVILESIFLKRSQQKKKTSPLNFKKRLFLLTVHKLSYYEYDFERGRRGSKKGSIDVEKITCVETVIPEKNPPPERQIPRRGEESSEMEQISIIERFPYPFQVVYDEGPLYVFSPTEELRKRWIHQLKNVIRYNSDLVQKYHPCFWIDGQYLCCSQTAKNAMGCQILENRNGSLKPGSSHRKTKKPLPPTPEEDQILKKPLPPEPTAAPISTTELKKVVALYDYMPMNANDLQLRKGEEYFILEESNLPWWRARDKNGQEGYIPSNYITEAEDSIEMYEWYSKHMTRSQAEQLLKQEGKEGGFIVRDSSKAGKYTVSVFAKSTGEPQGVIRHYVVCSTPQSQYYLAEKHLFSTIPELINYHQHNSAGLISRLKYPVSKQNKNAPSTAGLGYGSWEIDPKDLTFLKELGTGQFGVVKYGKWRGQYDVAIKMIREGSMSEDEFIEEAKVMMNLSHEKLVQLYGVCTKQRPIFIITEYMANGCLLNYLREMRHRFQTQQLLEMCKDVCEAMEYLESKQFLHRDLAARNCLVNDQGVVKVSDFGLSRYVLDDEYTSSVGSKFPVRWSPPEVLMYSKFSSKSDIWAFGVLMWEIYSLGKMPYERFTNSETAEHIAQGLRLYRPHLASERVYTIMYSCWHEKADERPSFKILLSNILDVMDEES E502B798BC36E223 txk_mouse Tyrosine-protein kinase TXK Txk Rlk PTK-RL-18 Resting lymphocyte kinase protein protein mouse Mus musculus Mouse MILSSYSSFQSVLCCCCCRCSVQKRQVRTQISLSREEELSEKHSQRQRPWFAKLMGKTQSNRGGVQPSKRKPLPPLPQEPPDERIQVKALYDFLPREPGNLALKRAEEYLILERCDPHWWKARDRFGNEGLIPSNYVTENRLANLEIYEWYHKNITRNQTERLLRQEAKEGAFIVRDSRHLGSYTISVFTRARRHTQSSIKHYQIKKNDSGQWYITERHLFPSVPELIQYHQYNAAGLISRLRYPIGLLGSCLPATSGFSYEKWEIDPSELAFVKEIGSGQFGVVHLGEWRAHIPVAIKAINEGSMSEEDFIEEAKVMMKLSHSRLVQLYGVCIQQKPLYIVTEFMENGCLLDYLRERKGQLQKALLLSMCQDICEGMAYLERSCYIHRDLAARNCLVSSACVVKISDFGMARYVLDDEYISSSGAKFPVKWCPPEVFHFNKYSSKSDVWSFGVLMWEVFTEGKMPFENKSNLQVVEAISQGFRLYRPHLAPMTIYRVMYSCWHESPKGRPTFAELLQVLTEIAETW D3A32C09B3A201FA itk_mouse Tyrosine-protein kinase ITK/TSK T-cell-specific kinase IL-2-inducible T-cell kinase Kinase EMT Kinase TLK Itk Emt Tlk Tsk protein protein mouse Mus musculus Mouse MNNFILLEEQLIKKSQQKRRTSPSNFKVRFFVLTKASLAYFEDRHGKKRTLKGSIELSRIKCVEIVKSDISIPCHYKYPFQTLVYLQVVHDNYLLYVFAPDCESRQRWVLTLKEETRNNNSLVSKYHPNFWMDGRWRCCSQLEKPAVGCAPYDPSKNASKKPLPPTPEDNRRSFQEPEETLVIALYDYQTNDPQELALRCDEEYYLLDSSEIHWWRVQDKNGHEGYAPSSYLVEKSPNNLETYEWYNKSISRDKAEKLLLDTGKEGAFMVRDSRTPGTYTVSVFTKAIISENPCIKHYHIKETNDSPKRYYVAEKYVFDSIPLLIQYHQYNGGGLVTRLRYPVCSWRQKAPVTAGLRYGKWVIQPSELTFVQEIGSGQFGLVHLGYWLNKDKVAIKTIQEGAMSEEDFIEEAEVMMKLSHPKLVQLYGVCLEQAPICLVFEFMEHGCLSDYLRSQRGLFAAETLLGMCLDVCEGMAYLEKACVIHRDLAARNCLVGENQVIKVSDFGMTRFVLDDQYTSSTGTKFPVKWASPEVFSFSRYSSKSDVWSFGVLMWEVFSEGKIPYENRSNSEVVEDISTGFRLYKPRLASCHVYQIMNHCWKEKPEDRPPFSQLLSQLAEIAEAGL DIP protein Q03526 original sequence version: 117 F7A4A18A8A1AADDC pi5p 1-phosphatidyl-1D-myo-inositol 5-phosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) phosphatidic acids phosphatidic acids small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 1-phosphatidyl-1d-myo-inositol 1-phosphatidyl-1D-myo-inositols small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) 1-phosphatidyl-1d-myo-inositol 3,4,5-trisphosphate 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphates small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) diglycerides diglycerides/DAG small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) btk-pi3p-1 2 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P35991:p.Glu348_Lys349delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain EK AA Thu Jul 07 12:16:45 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was glu348ala lys349ala Fri Jul 22 11:11:53 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'glu348ala,lys349ala' Wed May 02 16:05:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_lys348-349ala_ala' Tue Jun 30 18:21:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu348_Lys349delinsAlaAla' ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role bait bait direct interaction direct interaction 3a btk-pi4p-1 2 9 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P35991:p.Glu348_Lys349delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain EK AA Thu Jul 07 12:16:28 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was glu348ala lys349ala Fri Jul 22 10:56:58 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'glu348ala,lys349ala' Wed May 02 16:56:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_lys348-349ala_ala' Tue Jun 30 18:53:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu348_Lys349delinsAlaAla' ecoli Escherichia coli (strain K12) direct interaction direct interaction 3a btk-pi5p-1 2 9 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P35991:p.Glu348_Lys349delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain EK AA Thu Jul 07 12:16:35 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was glu348ala lys349ala Fri Jul 22 11:05:01 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'glu348ala,lys349ala' Wed May 02 18:57:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_lys348-349ala_ala' Tue Jun 30 16:29:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu348_Lys349delinsAlaAla' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 3a btk-pi35p2-1 2 4 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P35991:p.Glu348_Lys349delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain EK AA Thu Jul 07 12:16:56 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was glu348ala lys349ala Fri Jul 22 11:06:53 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'glu348ala,lys349ala' Wed May 02 18:39:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_lys348-349ala_ala' Tue Jun 30 15:56:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu348_Lys349delinsAlaAla' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3a btk-pi345p3-3 2 12 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey P35991:p.Glu348_Lys349delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain EK AA Thu Jul 07 12:16:57 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was glu348ala lys349ala Fri Jul 22 10:52:38 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'glu348ala,lys349ala' Wed May 02 18:47:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_lys348-349ala_ala' Tue Jun 30 17:12:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu348_Lys349delinsAlaAla' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3a btk-pi345p3-1 1 6 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P35991:p.Asn365Tyr mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain N Y Wed May 02 18:38:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn365tyr' Tue Jun 30 17:30:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn365Tyr' P35991:p.Arg372Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R G Wed May 02 16:13:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg372gly' Tue Jun 30 16:27:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg372Gly' P35991:p.Arg288Gln mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain R Q Wed May 02 20:32:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg288gln' Tue Jun 30 18:16:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg288Gln' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait direct interaction direct interaction 3b, 3c btk-pi-2 1 6 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P35991:p.Arg288Gln mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain R Q Wed May 02 15:14:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg288gln' Tue Jun 30 17:18:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg288Gln' P35991:p.Asn365Tyr mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain N Y Wed May 02 20:11:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn365tyr' Tue Jun 30 18:15:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn365Tyr' ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role bait bait direct interaction direct interaction 3b tyk-dag-1 1 7 unspecified role unspecified role prey prey region optyr O4'-phospho-L-tyrosine (2S)-2-amino-3-(4-phosphonooxyphenyl)propanoic acid 2-azanyl-3-(4-phosphonooxyphenyl)propanoic acid MOD_RES Phosphotyrosine O4-phosphotyrosine tyrosine phosphate 2-amino-3-(4-hydroxyphenyl)propanoic acid 4'-phosphate O4'-phospho-L-tyrosine O4'-phosphorylated L-tyrosine OPTyr Phospho Phospho Tyrosinyl Phosphorylation phosphorylationy range range range range region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role bait bait direct interaction direct interaction 3b itk-dag-1 1 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role bait bait direct interaction direct interaction 3b tyk-pa-1 1 7 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role bait bait direct interaction direct interaction 3b itk-pa-1 1 8 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind range range range range ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role bait bait direct interaction direct interaction 3b btk-pa-2 1 6 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P35991:p.Arg288Gln mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R Q Wed May 02 16:41:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg288gln' Tue Jun 30 19:43:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg288Gln' P35991:p.His362Gln mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain H Q Wed May 02 15:59:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his362gln' Tue Jun 30 17:49:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His362Gln' P35991:p.Asn365Tyr mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain N Y Wed May 02 17:24:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn365tyr' Tue Jun 30 16:41:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn365Tyr' P35991:p.Arg372Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R G Wed May 02 19:10:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg372gly' Tue Jun 30 16:00:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg372Gly' ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role bait bait direct interaction direct interaction 3b, 3c btk-pi45p2-1 2 6 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh3-sh2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role bait bait direct interaction direct interaction 3a btk-pi-1 2 11 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined sh3-sh2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction 3a btk-pa-1 2 6 unspecified role unspecified role prey prey sh3-sh2 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P35991:p.Glu348_Lys349delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain EK AA Thu Jul 07 12:15:57 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was glu348ala lys349ala Fri Jul 22 11:08:39 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'glu348ala,lys349ala' Wed May 02 20:01:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_lys348-349ala_ala' Tue Jun 30 18:12:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu348_Lys349delinsAlaAla' ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role bait bait direct interaction direct interaction 3a btk-dag-1 1 13 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P35991:p.Arg288Gln mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R Q Wed May 02 18:35:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg288gln' Tue Jun 30 18:07:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg288Gln' P35991:p.His362Gln mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain H Q Wed May 02 19:37:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his362gln' Tue Jun 30 20:16:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His362Gln' P35991:p.Asn365Tyr mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain N Y Wed May 02 18:02:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn365tyr' Tue Jun 30 18:06:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn365Tyr' P35991:p.Arg372Gly mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R G Wed May 02 16:41:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg372gly' Tue Jun 30 19:24:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg372Gly' ecoli Escherichia coli (strain K12) direct interaction direct interaction 3c btk-pi345p3-2 1 12 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P35991:p.Arg288Gln mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain R Q Wed May 02 17:25:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg288gln' Tue Jun 30 18:47:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg288Gln' P35991:p.Asn365Tyr mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain N Y Wed May 02 15:31:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn365tyr' Tue Jun 30 16:38:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn365Tyr' ecoli Escherichia coli (strain K12) direct interaction direct interaction 3c