IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Proteome-wide substrate analysis indicates substrate exclusion as a mechanism to generate caspase-7 versus caspase-3 specificity. Proteome-wide substrate analysis indicates substrate exclusion as a mechanism to generate caspase-7 versus caspase-3 specificity. Mol. Cell Proteomics (1535-9476) 2009 imex curation Demon D., Van Damme P., Vanden Berghe T., Deceuninck A., Van Durme J., Verspurten J., Helsens K., Impens F., Wejda M., Schymkowitz J., Rousseau F., Madder A., Vandekerckhove J., Declercq W., Gevaert K., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis In this experiment, mouse macrophages were metabolically labeled by SILACm lysed and cleaved by recombinant caspases. The cleaved samples were then analyzed via COFRADIC-coupled mass spectrometry. peter.vandenabeele@dmbr.UGent.be Only protein-protein interactions in vitro In vitro protease assay protease assay autoradiography autoradiography Demon D., Van Damme P., Vanden Berghe T., Deceuninck A., Van Durme J., Verspurten J., Helsens K., Impens F., Wejda M., Schymkowitz J., Rousseau F., Madder A., Vandekerckhove J., Declercq W., Gevaert K., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis In this experiment, mouse macrophages were metabolically labeled by SILACm lysed and cleaved by recombinant caspases. The cleaved samples were then analyzed via COFRADIC-coupled mass spectrometry. peter.vandenabeele@dmbr.UGent.be Accepted 2011-DEC-19 by JYOTI Mol. Cell Proteomics (1535-9476) 2009 Only protein-protein interactions imex curation Proteome-wide substrate analysis indicates substrate exclusion as a mechanism to generate caspase-7 versus caspase-3 specificity. Proteome-wide substrate analysis indicates substrate exclusion as a mechanism to generate caspase-7 versus caspase-3 specificity. Mol. Cell Proteomics (1535-9476) 2009 imex curation Demon D., Van Damme P., Vanden Berghe T., Deceuninck A., Van Durme J., Verspurten J., Helsens K., Impens F., Wejda M., Schymkowitz J., Rousseau F., Madder A., Vandekerckhove J., Declercq W., Gevaert K., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis In this experiment, mouse macrophages were metabolically labeled by SILACm lysed and cleaved by recombinant caspases. The cleaved samples were then analyzed via COFRADIC-coupled mass spectrometry. peter.vandenabeele@dmbr.UGent.be Only protein-protein interactions in vitro In vitro protease assay protease assay tag fluorescence tag visualisation by fluorescence tag fluorescence Demon D., Van Damme P., Vanden Berghe T., Deceuninck A., Van Durme J., Verspurten J., Helsens K., Impens F., Wejda M., Schymkowitz J., Rousseau F., Madder A., Vandekerckhove J., Declercq W., Gevaert K., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis In this experiment, mouse macrophages were metabolically labeled by SILACm lysed and cleaved by recombinant caspases. The cleaved samples were then analyzed via COFRADIC-coupled mass spectrometry. In this experiment, mouse macrophages were metabolically labeled by SILACm lysed and cleaved by recombinant caspases. The cleaved samples were then analyzed via COFRADIC-coupled mass spectrometry. peter.vandenabeele@dmbr.UGent.be Accepted 2011-DEC-19 by JYOTI Mol. Cell Proteomics (1535-9476) 2009 Only protein-protein interactions imex curation Proteome-wide substrate analysis indicates substrate exclusion as a mechanism to generate caspase-7 versus caspase-3 specificity. Proteome-wide substrate analysis indicates substrate exclusion as a mechanism to generate caspase-7 versus caspase-3 specificity. Mol. Cell Proteomics (1535-9476) 2009 imex curation Demon D., Van Damme P., Vanden Berghe T., Deceuninck A., Van Durme J., Verspurten J., Helsens K., Impens F., Wejda M., Schymkowitz J., Rousseau F., Madder A., Vandekerckhove J., Declercq W., Gevaert K., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis In this experiment, mouse macrophages were metabolically labeled by SILACm lysed and cleaved by recombinant caspases. The cleaved samples were then analyzed via COFRADIC-coupled mass spectrometry. peter.vandenabeele@dmbr.UGent.be Only protein-protein interactions in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Demon D., Van Damme P., Vanden Berghe T., Deceuninck A., Van Durme J., Verspurten J., Helsens K., Impens F., Wejda M., Schymkowitz J., Rousseau F., Madder A., Vandekerckhove J., Declercq W., Gevaert K., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis In this experiment, mouse macrophages were metabolically labeled by SILACm lysed and cleaved by recombinant caspases. The cleaved samples were then analyzed via COFRADIC-coupled mass spectrometry. peter.vandenabeele@dmbr.UGent.be Accepted 2011-DEC-19 by JYOTI Mol. Cell Proteomics (1535-9476) 2009 Only protein-protein interactions imex curation parp1_mouse Poly [ADP-ribose] polymerase 1 Poly[ADP-ribose] synthase 1 NAD(+) ADP-ribosyltransferase 1 Parp1 Adprp Adprt Adprt1 ADP-ribosyltransferase diphtheria toxin-like 1 DNA ADP-ribosyltransferase PARP1 Protein poly-ADP-ribosyltransferase PARP1 protein protein mouse Mus musculus Mouse MAEASERLYRVQYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGQSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVAGKGQDGSGGKAEKTLGDFAAEYAKSNRSMCKGCLEKIEKGQMRLSKKMVDPEKPQLGMIDRWYHPTCFVKKRDELGFRPEYSASQLKGFSLLSAEDKEALKKQLPAIKNEGKRKGDEVDGTDEVAKKKSRKETDKYSKLEKALKAQNELIWNIKDELKKACSTNDLKELLIFNQQQVPSGESAILDRVADGMAFGALLPCKECSGQLVFKSDAYYCTGDVTAWTKCMVKTQNPSRKEWVTPKEFREISYLKKLKVKKQDRIFPPESSAPITVHWPLSVTSAPTAVNSSAPADKPLSNMKILTLGKLSQNKDEAKAVIEKLGGKLTGSANKASLCISIKKEVEKMNKKMEEVKEANIRVVSEDFLQDVSASTKSLQDLLSAHSLSPWGAEVKAEPGEVVAPRGKSAAPSKKSKGCFKEEGVNKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKESRYWIFRSWGRLGTVIGSNKLEQMPSKEEAVEQFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVKPGTKSKLPKPVQELVGMIFDVDSMKKALVEYEIDLQKMPLGKLSRRQIQAAYSILSEVQQPVSQGSSESQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEVIRKYVKNTHATTHNAYDLEVIDIFKIEREGESQRYKPFRQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLIMLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSASITLEGVEVPLGTGIPSGVNDTALLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW DIP protein P11103 original sequence version: 122 DIP protein P11103 original sequence version: 163 DIP protein P11103 original sequence version: 164 5E54C3E5F60BB922 kpym_mouse Pyruvate kinase PKM Pkm Pk3 Pkm2 Pykm Pyruvate kinase muscle isozyme protein protein mouse Mus musculus Mouse MPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP DIP protein P52480 original sequence version: 159 34CBBC01BC0C047D casp7_mouse Caspase-7 Casp7 Lice2 Mch3 Apoptotic protease Mch-3 Cysteine protease LICE2 protein protein mouse Mus musculus Mouse MTDDQDCAAELEKVDSSSEDGVDAKPDRSSIISSILLKKKRNASAGPVRTGRDRVPTYLYRMDFQKMGKCIIINNKNFDKATGMDVRNGTDKDAGALFKCFQNLGFEVTVHNDCSCAKMQDLLRKASEEDHSNSACFACVLLSHGEEDLIYGKDGVTPIKDLTAHFRGDRCKTLLEKPKLFFIQACRGTELDDGIQADSGPINDIDANPRNKIPVEADFLFAYSTVPGYYSWRNPGKGSWFVQALCSILNEHGKDLEIMQILTRVNDRVARHFESQSDDPRFNEKKQIPCMVSMLTKELYFSR 747787B5BDE5F744 nucks_mouse Nuclear ubiquitous casein and cyclin-dependent kinase substrate 1 Nucks1 Nucks JC7 protein protein mouse Mus musculus Mouse MSRPVRNRKVVDYSQFQESDDADEDYGRDSGPPAKKIRSSPREAKNKRRSGKNSQEDSEDSEEKDVKTKKDDSHSAEDSEDEKDDHKNVRQQRQAASKAASKQREMLLEDVGSEEEPEEDDEAPFQEKDSGSDEDFLMEDDDDSDYGSSKKKNKKMVKKSKPERKEKKMPKPRLKATVTPSPVKGKAKVGRPTASKKSKEKTPSPKEEDEEAESPPEKKSGDEGSEDEASSGED CB2075863AA0E87A casp3_mouse Caspase-3 Casp3 Cpp32 Apopain Cysteine protease CPP32 Protein Yama SREBP cleavage activity 1 LICE protein protein mouse Mus musculus Mouse MENNKTSVDSKSINNFEVKTIHGSKSVDSGIYLDSSYKMDYPEMGICIIINNKNFHKSTGMSSRSGTDVDAANLRETFMGLKYQVRNKNDLTREDILELMDSVSKEDHSKRSSFVCVILSHGDEGVIYGTNGPVELKKLTSFFRGDYCRSLTGKPKLFIIQACRGTELDCGIETDSGTDEEMACQKIPVEADFLYAYSTAPGYYSWRNSKDGSWFIQSLCSMLKLYAHKLEFMHILTRVNRKVATEFESFSLDSTFHAKKQIPCIVSMLTKELYFYH DIP protein P70677 original sequence version: 99 DIP protein P70677 original sequence version: 110 CE91598F74826605 acta_mouse Actin, aortic smooth muscle Acta2 Actsa Actvs Alpha-actin-2 protein protein mouse Mus musculus Mouse MCEEEDSTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDEAGPSIVHRKCF 2D0543262DB35CA5 mk67i_mouse MKI67 FHA domain-interacting nucleolar phosphoprotein Nifk Mki67ip Nucleolar protein interacting with the FHA domain of pKI-67 protein protein mouse Mus musculus Mouse MAGLAGPAKPSLALNPQEDSQFEKALTQIQGRTKKPQQKKKEKLNRGVVYLGHLPSTLSESHIYNYCAQFGDISRFRLSRSKRTGNSKGYAFVEFESEDVAKIVAETMDNYLFGERLLSCKFMPRKKVHKDLFSQRNALFHRPSFPAVKRYNRKRGHLQMLKMEYRFKKKEKLLRKKLAAKGIDYSFPSLVLPKPKNIAVAHRDSEGNQVLPDQKEGLSGEPRRKEKMMKEDISNNIPKKRKRSRRKKSSVDSQGPTPVCTPTFLERRKSQVMEVGGDKDDEIILKLPVPPVKEDTQKTPTSASPGGKRPRKRKSKQ 08CC82E54EA2D580 mbb1a_mouse Myb-binding protein 1A Mybbp1a P160 Myb-binding protein of 160 kDa protein protein mouse Mus musculus Mouse MAEMKSPTKAEPATPAEAAQSDRHSLLEHSREFLDFFWDIAKPDQETRLRATEKLLEYLRTRPNDSEMKYALKRLITGLGVGREAARPCYSLALAQLLQSFEDIPLCDILDQIQEKYSLQAMNKAMMRPSLFANLFGVLALFQSGRLVKDKEALMKSVQLLKILSQHPNHLQGQPIKALVDILSEVPESMFQEILPKVLKGNMKVILRSPKYLELFLLAKQRVPTKLESLMGSVDLFSEDNIPSLVNILKVAANSVKKEHKLPNVALDLLRLALKESRFELFWKKVLEEGLLKNPSWTSSYMCFRLLGASLPLLSEEQLQLVMRGDLIRHFGENMVISKPQNLFKIIPEISTYVGTFLEGCQDDPKRQLTMMVAFTTITNQGLPVMPTFWRVTRFLNAEALQSYVAWLRDMFLQPDLNSLVDFSTANQKRAQDASLNVPERAVFRLRKWIIHRLVSLVDHLHLEKDEAVVEQIARFCLFHAFFKTKKATPQIPETKQHFSFPLDDRNRGVFVSAFFSLLQTLSVKFRQTPDLAENGKPWTYRLVQLADMLLNHNRNVTSVTSLTTQQRQAWDQMMSTLKELEARSSETRAIAFQHLLLLVGLHIFKSPAESCDVLGDIQTCIKKSMEQNPRRSRSRAKASQEPVWVEVMVEILLSLLAQPSNLMRQVVRSVFGHICPHLTPRCLQLILAVLSPVTNEDEDDNVVVTDDADEKQLQHGEDEDSDNEDNKNSESDMDSEDGEESEEEDRDKDVDPGFRQQLMEVLKAGNALGGVDNEEEEELGDEAMMALDQNLASLFKEQKMRIQARNEEKNKLQKEKKLRRDFQIRALDLIEVLVTKQPEHPLILELLEPLLNVIQHSMRSKGSTKQEQDLLHKTARIFMHHLCRARRYCHEVGPCAEALHAQVERLVQQAGSQADASVALYYFNASLYLLRVLKGNTNKRHQDGHKLHGADTEDSEDQAANCLDLDFVTRVYSASLESLLTKRNSSLTVPMFLSLFSRYPVICKNLLPVLAQHVAGPSRPRHQAQACLMLQKTLSARELRVCFEDPEWEQLITQLLGKATQTLQTLGEAQSKGEHQKELSILELLNTLLRTVNHEKLSVDLTAPLGVLQSKQQKLQQSLQQGNHSSGSNRLYDLYWQAMRMLGVQRPKSEKKNAKDIPSDTQSPVSTKRKKKGFLPETKKRKKLKSEGTTPEKNAASQQDAVTEGAMPAATGKDQPPSTGKKKRKRVKASTPSQVNGITGAKSPAPSNPTLSPSTPAKTPKLQKKKEKLSQVNGATPVSPIEPESKKHHQEALSTKEVIRKSPHPQSALPKKRARLSLVSRSPSLLQSGVKKRRVASRRVQTP DIP protein Q7TPV4 original sequence version: 72 64D3420981CD0767 rl28_mouse 60S ribosomal protein L28 Rpl28 protein protein mouse Mus musculus Mouse MSAHLQWMVVRNCSSFLIKRNKQTYSTEPNNLKARNSFRYNGLIHRKTVGVEPAADGKGVVVVMKRRSGQRKPATSYVRTTINKNARATLSSIRHMIRKNKYRPDLRMAAIRRASAILRSQKPVVVKRKRTRPTKSS DIP protein P41105 original sequence version: 96 44D9CF515F9B687B rs18_mouse 40S ribosomal protein S18 Rps18 Ke-3 protein protein mouse Mus musculus Mouse MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK 4DAF0662C3F37F22 wasc2_mouse WASH complex subunit 2 Washc2 D6Wsu116e Fam21 Kiaa0592 protein protein mouse Mus musculus Mouse MNRTSPDSERPPASEPVWERPWSVEEIRRSSQNWSLAADAGLLQFLQEFSQQTISRTHEIKKQVDGLIQETKATHCRLHNVFNDFLMLSNTQFIENRVYDEEVEEQVLKAEAEKAEQEKTREQKEIDLIPKVQEAVNYGLQVLDSAFEQLDIKAGNSDSEEDDANERVDLILEPKDLYIDRPLPYLIGSKLFMEQEDVGLGELSSEEGSVGSDRGSIVDSEDEKEEEESDEDFASHSDNDQNQHTTQISDEEEDDDGDLFADSEKEGDDIEDIEESAKSKRPTSFADELAARIKGDISNQRKEGQTDGKPQKTVKEKKERRTPADDEEDILFPPPTLTDEDFSPFGSRGGLFSNGQGLFDDEDESDLFKEAPRARPAQAPVSEELPPSPKPGKKIPAGAVSVLLGHPDVSGSTSAPSLKELQKHGQPTPGKSSHLPTPAGLFDDDDNDNDEDDNNFFMPSSSKPSKTDKVKSTAIIFDDDEGDLFKEKAEALPAASVSQTHESKTRADKTIALPSSKNLKLVSETKTQKGLFSDEEDSEDLFSSQSSSKPKSASLPSSQPPTSVSLFGDEDEEDSLFGSAAAKKQTSSLQPQSQEKAKPSEQPSKKTSALLFSSDEEDQWNIADSHTKLASDNKSKGELWDSGATQGQEAKAVKKTNLFEDDDDDEVDLFAIAKDSQKKTQRTSLLFEDDAESGSSLFGLPPTSVPSATTKKESVPKVPLLFSDEEDSEVPSGVKPEDLKVDNARVSPEVGSADVASIAQKEGLLPASDQEAGGPSDIFSSSSPLDKGAKGRTRTVLSLFDEDEDKVEDESSTCAPQDGREKGLKTDSRPKSTGVFQDEELLFSHKLQKDNDPDVDLFAGTKKIRSSVPSGGSLFGDDEDDDLFSSAKTQPVVPEKKGTLKKDHPVSLKNQDPLDSTQGSKEKSTWKTEPAQDSSGLTPFKSREPSSRIGKIQANLAINPAALLPTVALQIPGTKPVSSELAFPSSEPGRSHILESVPTLPGSVEAGVSFDLPAQADTLHSANKSRVKVRGKRRPQTRAARRLAAQESSEAEDVTVDRGPVAQLSSSPVLPNGHQPLLQPRMASGQTSSETATAPPWEGGPVLSAADRSFFVKSRPQTGNEADLFDSGDIFPKSRGSQSVEGAGVMAGEPPSHSSGGRKEKSLAFPDLSEGSSTEDLFQSVKPRAAKNRNPFPLLEDEEDLFADPRGKKNERKPDSHQDSVSKTHDIFEDDIFATEAIKPFPKKREKGRTLEPNLFDDNIDIFADLTVKPKEKSKKKVAAKSMFDDDTDDIFSSGLQAKASKPKSQSAEAASEQRSEHKVASIFDDPLNAFGSQ AD35C3FFAC27C159 hdgf_mouse Hepatoma-derived growth factor Hdgf Tdrm1 protein protein mouse Mus musculus Mouse MSRSNRQKEYKCGDLVFAKMKGYPHWPARIDEMPEAAVKSTANKYQVFFFGTHETAFLGPKDLFPYEESKEKFGKPNKRKGFSEGLWEIENNPTVKASGYQSSQKKSCAAEPEVEPEAHEGDGDKKGSAEGSSDEEGKLVIDEPAKEKNEKGTLKRRAGDVLEDSPKRPKESGDHEEEDKEIAALEGERPLPVEVEKNSTPSEPDSGQGPPAEEEEGEEEAAKEEAEAQGVRDHESL AAE4CF574DA4733F casp7-rps18-1 1 6 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage 2a, 5c and 6e casp7-mk67i-1 1 10 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 6 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 2b casp7-mybbp1a 1 6 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 11 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage 2c casp7-rl28 1 12 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 6 enzyme enzyme unspecified role unspecified role purified purified casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 2d casp7-nucks 1 7 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 6 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 2f casp7-hdgf 1 6 enzyme enzyme unspecified role unspecified role purified purified casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 15 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage 2f casp7-fam21 1 6 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 14 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage 2h casp7-acta-1 1 6 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 9 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage 2i casp7-kpym-1 1 6 enzyme enzyme unspecified role unspecified role purified purified casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 5 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage 2j casp3-kpym-1 1 5 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 8 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 2j casp3-acta2-1 1 9 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 8 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 2i casp3-fam21-1 1 8 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 14 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage 2h casp3-hdgf-1 1 15 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 8 enzyme enzyme unspecified role unspecified role purified purified casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 2g casp3-nucks-1 1 8 enzyme enzyme unspecified role unspecified role purified purified casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 7 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage 2f casp7-rps18-2 3 13 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified p6-p5' undecapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pentapeptide sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 6 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 4a, 5a, 6a, 6b, 6c, 6d and 6e casp3-rps18-2 1 8 enzyme enzyme unspecified role unspecified role purified purified casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P62270:p.Lys88Ala mutation mutation certain certain sequence position certain certain certain sequence position certain K A Wed May 02 15:59:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys88ala' Tue Jun 30 17:25:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys88Ala' This mutation is required for casp3 to be able to cleave this peptide. rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage 5d casp3-parp 3 8 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 4 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified P11103:p.Gly210Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G K Wed May 02 16:23:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly210lys' Tue Jun 30 19:37:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly210Lys' This mutation is required for casp3 to be able to cleave this peptide. p6-p5' undecapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tetrapeptide sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage 5f and 6h casp7-parp 3 6 enzyme enzyme unspecified role unspecified role purified purified casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 4 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified p6-p5' undecapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tetrapeptide sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage 5e and 6f casp3-rps18-1 3 13 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified p6-p5' undecapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P62270:p.Lys88Ala mutation mutation certain certain sequence position certain certain certain sequence position certain K A Wed May 02 15:27:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys88ala' Tue Jun 30 17:25:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys88Ala' This mutation is required for casp3 to be able to cleave this peptide. chemical synthesis Chemical synthesis (Chemical synthesis) 8 enzyme enzyme unspecified role unspecified role purified purified casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 149 150 protein cleavage protein cleavage 5b casp7-parp1 1 6 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 4 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage 5g and 6f casp3-parp1-1 1 8 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 4 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified P11103:p.Gly210Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G K Wed May 02 18:37:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly210lys' Tue Jun 30 18:45:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly210Lys' This mutation is required for casp3 to be able to cleave this peptide. [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage 5h and 6h casp7-parp1-1 2 4 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified tetrapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain amc fluorogenic tag fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) 6 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) protein cleavage protein cleavage table 1 casp3-parp1-2 2 8 enzyme enzyme unspecified role unspecified role purified purified strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 4 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified tetrapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain amc fluorogenic tag fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage table 1 casp7-rps18-3 2 6 enzyme enzyme unspecified role unspecified role purified purified casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified p6-p5' undecapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The peptide has a Y(NO2) tag in the C-terminus. amc fluorogenic tag fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range tetrapeptide sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain kcat = 0.23 s-1 Km = 53 microM chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage table 1 casp7-rps18-4 2 13 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified amc fluorogenic tag fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range pentapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The peptide has a Y(NO2) tag in the C-terminus. P62270:p.Lys88Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A Wed May 02 20:17:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys88ala' Tue Jun 30 20:22:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys88Ala' chemical synthesis Chemical synthesis (Chemical synthesis) 6 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range casp7 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) protein cleavage protein cleavage table 1 casp3-rps18-5 2 13 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified tetrapeptide sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain kcat = 0.74 s-1 Km = 267 microM amc fluorogenic tag fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range hexapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The peptide has a Y(NO2) tag in the C-terminus. P62270:p.Gln87Ala mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q A Wed May 02 16:48:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln87ala' Tue Jun 30 17:08:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln87Ala' chemical synthesis Chemical synthesis (Chemical synthesis) 8 enzyme enzyme unspecified role unspecified role purified purified casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) protein cleavage protein cleavage table 1 casp3-rps18-6 2 13 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified P62270:p.Lys88Ala mutation increasing mutation increasing interaction mutation increasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:22:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys88ala' Tue Jun 30 16:03:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys88Ala' amc fluorogenic tag fluorophore fluorophore n-term range n-terminal range n-term range n-term range n-terminal range n-term range pentapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain The peptide has a Y(NO2) tag in the C-terminus. chemical synthesis Chemical synthesis (Chemical synthesis) 8 enzyme enzyme unspecified role unspecified role purified purified casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) protein cleavage protein cleavage table 1 casp3-rps18-7 3 8 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range strep tag strep tag strep tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range casp3 without prodomain sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified p6-p2' octapeptide sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain pentapeptide sufficient to bind sufficient binding region sufficient to bind mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain The interaction is increased with this shortened peptide. chemical synthesis Chemical synthesis (Chemical synthesis) protein cleavage protein cleavage 6c, 6d and 6g