IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. Mol. Cell (1097-2765) 2009 imex curation Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA jyoti Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no Accepted 2010-APR-17 by JYOTI. 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA Mol. Cell (1097-2765) 2009 jyoti Only protein-protein interactions imex curation A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. Mol. Cell (1097-2765) 2009 imex curation Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA jyoti Only protein-protein interactions mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no Accepted 2010-APR-17 by JYOTI. 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA Mol. Cell (1097-2765) 2009 jyoti Only protein-protein interactions imex curation A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. Mol. Cell (1097-2765) 2009 imex curation Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA jyoti Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no Accepted 2010-APR-17 by JYOTI. 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA Mol. Cell (1097-2765) 2009 jyoti Only protein-protein interactions imex curation A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. Mol. Cell (1097-2765) 2009 imex curation Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no Accepted 2010-APR-17 by JYOTI. 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA Mol. Cell (1097-2765) 2009 jyoti Only protein-protein interactions imex curation A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. Mol. Cell (1097-2765) 2009 imex curation Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA jyoti Only protein-protein interactions mouse-hepatocyte Mouse hepatocyte hepatocyte Liver primary cell A hepatocyte is a cell of the main tissue of the liver. confocal microscopy confocal microscopy immunostaining immunostaining Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no Accepted 2010-APR-17 by JYOTI. 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA Mol. Cell (1097-2765) 2009 jyoti Only protein-protein interactions imex curation A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. Mol. Cell (1097-2765) 2009 imex curation Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA jyoti Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no Accepted 2010-APR-17 by JYOTI. 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA Mol. Cell (1097-2765) 2009 jyoti Only protein-protein interactions imex curation A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. Mol. Cell (1097-2765) 2009 imex curation Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA jyoti Only protein-protein interactions mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. confocal microscopy confocal microscopy immunostaining immunostaining Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no Accepted 2010-APR-17 by JYOTI. 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA Mol. Cell (1097-2765) 2009 jyoti Only protein-protein interactions imex curation A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. Mol. Cell (1097-2765) 2009 imex curation Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA jyoti Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no Accepted 2010-APR-17 by JYOTI. 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA Mol. Cell (1097-2765) 2009 jyoti Only protein-protein interactions imex curation A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. Mol. Cell (1097-2765) 2009 imex curation Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA jyoti Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no Accepted 2010-APR-17 by JYOTI. 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA Mol. Cell (1097-2765) 2009 jyoti Only protein-protein interactions imex curation A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. Mol. Cell (1097-2765) 2009 imex curation Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA jyoti Only protein-protein interactions mouse-liver Mus musculus liver liver anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no Accepted 2010-APR-17 by JYOTI. 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA Mol. Cell (1097-2765) 2009 jyoti Only protein-protein interactions imex curation A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. Mol. Cell (1097-2765) 2009 imex curation Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA jyoti Only protein-protein interactions human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no Accepted 2010-APR-17 by JYOTI. 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA Mol. Cell (1097-2765) 2009 jyoti Only protein-protein interactions imex curation A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. A role for NBR1 in autophagosomal degradation of ubiquitinated substrates. Mol. Cell (1097-2765) 2009 imex curation Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA jyoti Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Kirkin V., Lamark T., Sou YS., Bjorkoy G., Nunn JL., Bruun JA., Shvets E., McEwan DG., Clausen TH., Wild P., Bilusic I., Theurillat JP., Overvatn A., Ishii T., Elazar Z., Komatsu M., Dikic I., Johansen T. Apoptosis - Interactions involving proteins with a function related to apoptosis GFP-LC3 Tg:SQSTM1-/- mice used in this study ivan.dikic@biochem2.de, terjej@fagmed.uit.no Accepted 2010-APR-17 by JYOTI. 29-Mar-2010: Contacted by JYOTI. Human thymus cDNA Mol. Cell (1097-2765) 2009 jyoti Only protein-protein interactions imex curation atg8_yeast Autophagy-related protein 8 ATG8 APG8 AUT7 YBL078C YBL0732 CVT5 Autophagy-related ubiquitin-like modifier ATG8 Cytoplasm to vacuole targeting protein 5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKSTFKSEYPFEKRKAESERIADRFKNRIPVICEKAEKSDIPEIDKRKYLVPADLTVGQFVYVIRKRIMLPPEKAIFIFVNDTLPPTAALMSAIYQEHKDKDGFLYVTYSGENTFGR 1246CEFF5A038819 1246CEFF5A038819 nbr1_human Next to BRCA1 gene 1 protein Neighbor of BRCA1 gene 1 protein Membrane component chromosome 17 surface marker 2 Protein 1A1-3B NBR1 1A13B KIAA0049 M17S2 Cell migration-inducing gene 19 protein MIG19 protein protein human Homo sapiens Human MEPQVTLNVTFKNEIQSFLVSDPENTTWADIEAMVKVSFDLNTIQIKYLDEENEEVSINSQGEYEEALKMAVKQGNQLQMQVHEGHHVVDEAPPPVVGAKRLAARAGKKPLAHYSSLVRVLGSDMKTPEDPAVQSFPLVPCDTDQPQDKPPDWFTSYLETFREQVVNETVEKLEQKLHEKLVLQNPSLGSCPSEVSMPTSEETLFLPENQFSWHIACNNCQRRIVGVRYQCSLCPSYNICEDCEAGPYGHDTNHVLLKLRRPVVGSSEPFCHSKYSTPRLPAALEQVRLQKQVDKNFLKAEKQRLRAEKKQRKAEVKELKKQLKLHRKIHLWNSIHGLQSPKSPLGRPESLLQSNTLMLPLQPCTSVMPMLSAAFVDENLPDGTHLQPGTKFIKHWRMKNTGNVKWSADTKLKFMWGNLTLASTEKKDVLVPCLKAGHVGVVSVEFIAPALEGTYTSHWRLSHKGQQFGPRVWCSIIVDPFPSEESPDNIEKGMISSSKTDDLTCQQEETFLLAKEERQLGEVTEQTEGTAACIPQKAKNVASERELYIPSVDLLTAQDLLSFELLDINIVQELERVPHNTPVDVTPCMSPLPHDSPLIEKPGLGQIEEENEGAGFKALPDSMVSVKRKAENIASVEEAEEDLSGTQFVCETVIRSLTLDAAPDHNPPCRQKSLQMTFALPEGPLGNEKEEIIHIAEEEAVMEEEEDEEDEEEEDELKDEVQSQSSASSEDYIIILPECFDTSRPLGDSMYSSALSQPGLERGAEGKPGVEAGQEPAEAGERLPGGENQPQEHSISDILTTSQTLETVPLIPEVVELPPSLPRSSPCVHHHGSPGVDLPVTIPEVSSVPDQIRGEPRGSSGLVNSRQKSYDHSRHHHGSSIAGGLVKGALSVAASAYKALFAGPPVTAQPIISEDQTAALMAHLFEMGFCDRQLNLRLLKKHNYNILQVVTELLQLNNNDWYSQRY 6A057E67947838EF sqstm_human Sequestosome-1 SQSTM1 ORCA OSIL Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa Ubiquitin-binding protein p62 EBI3-associated protein of 60 kDa protein protein human Homo sapiens Human MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL DIP protein Q13501 original sequence version: 139 DIP protein Q13501 original sequence version: 142 DIP protein Q13501 original sequence version: 163 DIP protein Q13501 original sequence version: 146 DIP protein Q13501 original sequence version: 162 DIP protein Q13501 original sequence version: 155 DIP protein Q13501 original sequence version: 140 462D94C171F337CD gbrl2_human Gamma-aminobutyric acid receptor-associated protein-like 2 GABA(A) receptor-associated protein-like 2 Golgi-associated ATPase enhancer of 16 kDa General protein transport factor p16 Ganglioside expression factor 2 MAP1 light chain 3-related protein GABARAPL2 FLC3A GEF2 protein protein human Homo sapiens Human MKWMFKEDHSLEHRCVESAKIRAKYPDRVPVIVEKVSGSQIVDIDKRKYLVPSDITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEKDEDGFLYVAYSGENTFGF DIP protein P60520 original sequence version: 93 DIP protein P60520 original sequence version: 112 17ACB540FD5B975B gbrap_human Gamma-aminobutyric acid receptor-associated protein GABA(A) receptor-associated protein MM46 GABARAP FLC3B HT004 protein protein human Homo sapiens Human MKFVYKEEHPFEKRRSEGEKIRKKYPDRVPVIVEKAPKARIGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRAEDALFFFVNNVIPPTSATMGQLYQEHHEEDFFLYIAYSDESVYGL DIP protein O95166 original sequence version: 109 DIP protein O95166 original sequence version: 112 DIP protein O95166 original sequence version: 128 DIP protein O95166 original sequence version: 132 BC0B84B8A51C1E32 sqstm_mouse Sequestosome-1 Ubiquitin-binding protein p62 STONE14 Sqstm1 A170 STAP protein protein mouse Mus musculus Mouse MASFTVKAYLLGKEEATREIRRFSFCFSPEPEAEAQAAAGPGPCERLLSRVAVLFPTLRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRREHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHREHSKLIFPNPFGHLSDSFSHSRWLRKLKHGHFGWPGWEMGPPGNWSPRPPRAGDGRPCPTAESASAPPEDPNVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPTTPESSSTGTEDKSNTQPSSCSSEVSKPDGAGEGPAQSLTEQMKKIALESVGQPEEQMESGNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL DIP protein Q64337 original sequence version: 121 DIP protein Q64337 original sequence version: 130 ED4CCCA7741D35CA ubb_human Polyubiquitin-B UBB protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGC 33011162F1C48BB1 ubiq_mouse Ubiquitin Rps27a Uba80 Ubcep1 Uba52 Ubcep2 Ubb Ubc protein protein mouse Mus musculus Mouse MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG C42A35397FFD9B52 gbrl1_human Gamma-aminobutyric acid receptor-associated protein-like 1 GABA(A) receptor-associated protein-like 1 Glandular epithelial cell protein 1 Early estrogen-regulated protein GABARAPL1 GEC1 protein protein human Homo sapiens Human MKFQYKEDHPFEYRKKEGEKIRKKYPDRVPVIVEKAPKARVPDLDKRKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNTIPPTSATMGQLYEDNHEEDYFLYVAYSDESVYGK DIP protein Q9H0R8 original sequence version: 129 7F56E345255F564B mlp3b_human Microtubule-associated proteins 1A/1B light chain 3B MAP1LC3B MAP1ALC3 Microtubule-associated protein 1 light chain 3 beta MAP1A/MAP1B light chain 3 B MAP1 light chain 3-like protein 2 Autophagy-related protein LC3 B Autophagy-related ubiquitin-like modifier LC3 B protein protein human Homo sapiens Human MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDGFLYMVYASQETFGMKLSV DIP protein Q9GZQ8 original sequence version: 69 DIP protein Q9GZQ8 original sequence version: 108 DIP protein Q9GZQ8 original sequence version: 129 DIP protein Q9GZQ8 original sequence version: 132 DIP protein Q9GZQ8 original sequence version: 148 DIP protein Q9GZQ8 original sequence version: 152 DIP protein Q9GZQ8 original sequence version: 111 DIP protein Q9GZQ8 original sequence version: 153 BB141DEC7653E83F mlp3a_human Microtubule-associated proteins 1A/1B light chain 3A Microtubule-associated protein 1 light chain 3 alpha MAP1A/MAP1B light chain 3 A MAP1 light chain 3-like protein 1 Autophagy-related protein LC3 A Autophagy-related ubiquitin-like modifier LC3 A MAP1LC3A protein protein human Homo sapiens Human MPSDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADIYEQEKDEDGFLYMVYASQETFGF 48C1FBE8F7892AF3 nbr1_mouse Next to BRCA1 gene 1 protein Neighbor of BRCA1 gene 1 protein Membrane component chromosome 17 surface marker 2 homolog Nbr1 M17s2 protein protein mouse Mus musculus Mouse MEPQVTLNVTFKNETQSFLVSDPENTTWADVEAMVKVSFDLNTIQIKYLDEENEEISINSQGEYEEALKMANIKQGNQLQMQVHEGYHVVDEALPKNVVENQAAARTGKKPLAHYSSLVRVLGSDMKTTEEPAPEQCSSAPCDTDQPQDKPPDWFTSYLEMFREQVVKETVEKLEQRLQEKLVLQKPLLSSSPTEVSMPISEETLFLPENQFSWHIACSHCQKRIVGVRYQCSLCPSYNICEDCEAGPYTHDTNHVLLKLRRPVVISSEPFFYSKYSAPRLPAALEQVRLQKQVDKNFVKAEKQRLRAEKKQRKAEVKELKKQLKLHRKIHLWNSIHGLQSPKSPLGRPESLLQSNTLMLPLQPCAPVMPTLSAAFVDENLPDGTHLQPGTKFIKHWRMKNTGNVKWNTDTKLKFMWGNLTLASTEKKDVLVPCLKAGHVGVVSVEFIAPTLEGTYTSHWRLSHKGQQFGPRVWCSIIVDPFPSSESPDNVEGDRISSSKADDFSCEQEEAFLLAEEEIPLGEVTKQTEGTGASASQKTRRAASERELYIPSVDLLTAQDLLSFELLDINIVQELERVPHNTPVDMTPCMSPLPHDSPLIEKPGLGQIQEESEGAGFKAPPDSTVSAKRKAETPASVEETEEDLSGTQFVCETVIRSLTLDAAPDHNPPCRQRSPQRELQLYSTEGQQPLVLPGFCRKDSSLKFALPEEGPRGDEREEIVHIVEEEVVEEEEEVQDEEVQSQSSASSEDYIIILPECFDTSRPLGDSMYSSALSQPGLERGAEGEPGIESGLEPTEARERLPERESQPQEQSISDILTTSQPLDTVPLVPEVAGLPAALSRSAPCGQCESSGVDSPGVDSPATMHEVPPAPDDIRGEPRGSTGLANSRQRSCDHSRHHNGSSIAGGLVKGALSVAASAYKALFSGPPVTAQPIVSEDQTTALMAHLFEMGFCDRQLNLRLLRKHNYNILQVVTELLQVNNNDWYSHRY E5BB4B281FE33CE0 mlp3c_human Microtubule-associated proteins 1A/1B light chain 3C MAP1LC3C Microtubule-associated protein 1 light chain 3 gamma MAP1A/MAP1B light chain 3 C MAP1 light chain 3-like protein 3 Autophagy-related protein LC3 C Autophagy-related ubiquitin-like modifier LC3 C protein protein human Homo sapiens Human MPPPQKIPSVRPFKQRKSLAIRQEEVAGIRAKFPNKIPVVVERYPRETFLPPLDKTKFLVPQELTMTQFLSIIRSRMVLRATEAFYLLVNNKSLVSMSATMAEIYRDYKDEDGFVYMTYASQETFGCLESAAPRDGSSLEDRPCNPL DIP protein Q9BXW4 original sequence version: 92 DIP protein Q9BXW4 original sequence version: 111 D3DFA8EE5985C3AA atg8-nbr1-1 8 13 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association Identification of NBR1 as an Interaction Partner for ATG8 Family Proteins Fig. 1A atg8-sqstm1-1 8 15 unspecified role unspecified role prey prey 13 unspecified role unspecified role bait bait physical association physical association Importantly, p62 was also picked in the ATG8 screen (Figure 1A). Fig. 1A map1lc3b-sqstm1-7 8 22 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey physical association physical association only p62 was recovered as an LC3 interaction partner Fig. 1A gabarapl2-nbr1-1 8 16 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey physical association physical association In additional yeast-two-hybrid screens, NBR1 and p62 were identified as GABARAPL2 interacting proteins Fig. 1A sqstm1-nbr1-2 6 15 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization GFP-tagged wild type, but not D50R mutant, of NBR1 colocalizes with endogenous p62 in cytoplasmic speckles in transfected HeLa cells. Supp. Fig. 1B atg8-nbr1-2 4 13 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association We verified the observed interaction between NBR1 and scATG8 in yeast by retransforming the isolated prey vector encoding a partial C-terminal sequence (amino acids 691�966) of NBR1 into the yeast strain expressing scATG8 as bait (Figure 1B). Fig. 1B atg8-sqstm1-2 4 13 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1B gabarapl2-nbr1-2 4 16 unspecified role unspecified role bait bait 14 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1B map1lc3b-sqstm1-8 4 22 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1B gabarapl2-sqstm1-2 8 15 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait physical association physical association In additional yeast-two-hybrid screens, NBR1 and p62 were identified as GABARAPL2 interacting proteins Fig. 1A gabarapl2-sqstm1-3 4 16 unspecified role unspecified role bait bait 15 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 1B atg8-nbr1-3 12 14 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 13 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 1C, Supp Fog. 1c Binding is not altered by a Asp50Arg mutation in NBR1. gabarap-nbr1-1 12 14 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 17 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 1C, Supp. Fig. 1C Binding is not altered by a Asp50Arg mutation in NBR1. gabarapl1-nbr1-1 12 14 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 21 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 1C, Supp. Fig. 1C Binding is not altered by a Asp50Arg mutation in NBR1. gabarapl2-nbr1-3 12 14 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 16 unspecified role unspecified role bait bait lir region binding region binding-associated region binding region deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-2606046]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 727-727, sequence length 117 [EBI-2606046]727-738 lir2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-2606048]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 540-540, sequence length 117 [EBI-2606048]540-636 region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 1C, Supp. Figs. 1C, 2D, 2E Binding is not altered by a Asp50Arg mutation in NBR1. ATG family proteins contain 2 NBR1 binding sites and will tolerate the loss of either but not both. map1lc3a-nbr1-1 12 14 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 23 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 1C, Supp. Fig. 1C Binding is not altered by a Asp50Arg mutation in NBR1. map1lc3b-nbr1-1 12 22 unspecified role unspecified role bait bait lir region binding region binding-associated region binding region deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-2606039]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 727-727, sequence length 125 [EBI-2606039]727-738 lir2 region binding region binding-associated region binding region deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-2606041]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 540-540, sequence length 125 [EBI-2606041]540-636 region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 1C, Supp. Figs. 1C, 2D, 2E Binding is not altered by a Asp50Arg mutation in NBR1. ATG family proteins contain 2 NBR1 binding sites and will tolerate the loss of either but not both. map1lc3c-nbr1-1 12 14 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 25 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 1C, Supp. Fig. 1C Binding is not altered by a Asp50Arg mutation in NBR1. map1lc3b-sqstm1-9 12 15 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 22 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 1C map1lc3c-sqstm1-1 12 15 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 25 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 1C gabarapl1-sqstm1-1 12 21 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 15 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 1C atg8-sqstm1-3 12 13 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 15 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 1C map1lc3a-sqstm1-1 12 15 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 23 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 1C gabarap-sqstm1-1 12 17 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 15 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 1C gabarapl2-sqstm1-4 12 15 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 16 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Fig. 1C atg8-nbr1-4 9 13 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association Fig. 1D gabarap-nbr1-2 9 17 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role prey prey Q14596:p.Tyr732Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 18:34:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr732ala' Tue Jun 30 19:22:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr732Ala' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Figs. 1D, 3C, Supp. Fig. 1D Binding was reduced but not preventing when region 540-636 was removed from NBR1 suggesting the existence of 2 binding sites. gabarapl1-nbr1-2 9 14 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain in vitro In vitro 21 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1D gabarapl2-nbr1-4 9 16 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role prey prey Q14596:p.Tyr732Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 15:36:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr732ala' Tue Jun 30 18:09:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr732Ala' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction Figs. 1D, 3C, Supp. Fig. 1D Binding was reduced but not preventing when region 540-636 was removed from NBR1 suggesting the existence of 2 binding sites. map1lc3a-nbr1-2 9 14 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 23 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1D map1lc3b-nbr1-2 9 14 unspecified role unspecified role prey prey Q14596:p.Tyr732Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 14:59:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr732ala' Tue Jun 30 15:55:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr732Ala' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 22 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1D, 3B Binding was reduced but not preventing when region 540-636 was removed from NBR1 suggesting the existence of 2 binding sites. map1lc3c-nbr1-2 9 25 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction Fig. 1D map1lc3b-nbr1-3 1 14 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 22 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1E gabarap-nbr1-3 1 14 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 17 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1E gabarapl2-nbr1-5 1 16 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. direct interaction direct interaction Fig. 1D gabarap-sqstm1-2 1 17 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 15 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. direct interaction direct interaction Fig. 1E gabarapl2-sqstm1-5 1 16 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 15 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. direct interaction direct interaction Fig. 1D map1lc3b-sqstm1-10 1 15 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 22 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1E map1lc3b-sqstm1-11 2 18 unspecified role unspecified role prey prey 22 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 2A map1lc3b-nbr1-4 2 24 unspecified role unspecified role prey prey 22 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 2A Interaction observed in SQSTM1 -/- cells. gabarapl2-nbr1-6 2 24 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 2A Interaction observed in the SQSTM1 -/- mice. gabarapl2-sqstm1-6 2 18 unspecified role unspecified role prey prey 16 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 2A map1lc3b-nbr1-5 10 24 unspecified role unspecified role prey prey 22 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 2C Interaction observed in SQSTM1 -/- cells. atg8-nbr1-5 1 14 unspecified role unspecified role bait bait Q14596:p.Tyr732Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 18:39:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr732ala' Tue Jun 30 19:24:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr732Ala' region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 13 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Fig. 3B, Supp. Fig. 2C Deletion studies suggest there are 2 binding sites for ATG family proteins on NBR1 gabarapl2-nbr1-7 1 14 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q14596:p.Tyr732Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 20:24:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr732ala' Tue Jun 30 16:51:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr732Ala' ecoli Escherichia coli (strain K12) 16 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3B, Supp.Fig 2C Deletion mutant studies suggest there are two binding sites for NBR1 on ATG family proteins. map1lc3b-nbr1-6 1 14 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q14596:p.Tyr732Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y A Wed May 02 17:58:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr732ala' Tue Jun 30 18:26:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr732Ala' region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 22 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3C, Supp. Fig. 2C Deletion analysis suggests 2 binding sites for ATG family members on NBR1. map1lc3b-nbr1-7 9 22 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9GZQ8:p.Arg10_Arg11delinsAlaAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain RR AA Wed May 02 19:22:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg_arg10-11ala_ala' Tue Jun 30 19:57:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg10_Arg11delinsAlaAla' Q9GZQ8:p.Arg70Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 16:17:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg70ala' Tue Jun 30 17:39:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg70Ala' ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction Fig. 3D GST pull-down assays using in vitro translated (35S)-labeled HA-NBR1(D50R)(ΔCC1) or HA-p62(R21A/D69A) and immobilized GST or indicated GST-LC3B constructs. nbr1-sqstm1-1 11 14 unspecified role unspecified role prey prey Q14596:p.Asp50Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D R Wed May 02 14:40:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp50arg' Tue Jun 30 15:45:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp50Arg' region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 1A HA-tagged p62 or p62(K7A) was immunoprecipitated (with HA-antibody) from lysates of HEK293 cells expressing the indicated p62 and NBR1 constructs, and co-purifying NBR1 or NBR1(D50R) was detected by western blot (with GFP-antibody). nbr1-sqstm1-2 11 15 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q13501:p.Lys7Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A Wed May 02 15:35:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys7ala' Tue Jun 30 16:57:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys7Ala' 14 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 1A HA-tagged p62 or p62(K7A) was immunoprecipitated (with HA-antibody) from lysates of HEK293 cells expressing the indicated p62 and NBR1 constructs, and co-purifying NBR1 or NBR1(D50R) was detected by western blot (with GFP-antibody). atg8-nbr1-6 1 13 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 14 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. physical association physical association Supp. Fig. 2B map1lc3b-nbr1-9 1 14 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 22 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Supp. Fig. 2B gabarapl2-nbr1-8 1 14 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag deletion analysis deletion analysis undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 16 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association Supp. Fig. 2B map1lc3b-nbr1-10 6 14 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 22 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Supp. Fig. 3 LIR, but not "LIR2", is essential for co-localization of mCherry-NBR1 with GFP-LC3B sqstm1-nbr1-1 7 18 unspecified role unspecified role neutral component neutral component 24 unspecified role unspecified role neutral component neutral component colocalization colocalization Wild type and p62-/- MEFs were left untreated or treated with 0,2 μM Bafilomycin A1 for 12h, fixed and stained for Nbr1 (monoclonal antibody) and p62 (guinea pig polyclonal antibody). Supp. Fig. 4 sqstm1-nbr1-3 5 24 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component colocalization colocalization NBR1 co-localizes with p62 and Ub in inclusions in autophagy-deficient (ATG7-/-) hepatocytes. Supp. Fig. 5 rps27a-nbr1-1 5 24 unspecified role unspecified role neutral component neutral component 20 unspecified role unspecified role neutral component neutral component colocalization colocalization NBR1 co-localizes with p62 and Ub in inclusions in autophagy-deficient (ATG7-/-) hepatocytes. Supp. Fig. 5 sqstm1-nbr1-4 3 14 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component colocalization colocalization Supp. Fig. 8 rps27a-nbr1-2 3 19 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component colocalization colocalization Supp. Fig. 8