UniProt UniProt http://www.uniprot.org SIRT3 interacts with the daf-16 homolog FOXO3a in the Mitochondria, as well as increases FOXO3a Dependent Gene expression. SIRT3 interacts with the daf-16 homolog FOXO3a in the Mitochondria, as well as increases FOXO3a Dependent Gene expression. Int. J. Biol. Sci. 2008 Jacobs K.M., Pennington J.D., Bisht K.S., Aykin-Burns N., Kim H.S., Mishra M., Sun L., Nguyen P., Ahn B.H., Leclerc J., Deng C.X., Spitz D.R., Gius D. Diabetes - Interactions investigated in the context of Diabetes Apoptosis - Interactions involving proteins with a function related to apoptosis fig 1A shows interaction of transfected hSIRT3 with COS7 endogenous FOX3O3a for which no seq is available. HCT116 cells, which contain undetectable levels of endogenous SIRT3 by western analysis, were stably transfected with p-myc-hSIRT3-wt. giusd@mail.nih.gov 16-Dec-2008: Contacted by JYOTI. davet human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Jacobs K.M., Pennington J.D., Bisht K.S., Aykin-Burns N., Kim H.S., Mishra M., Sun L., Nguyen P., Ahn B.H., Leclerc J., Deng C.X., Spitz D.R., Gius D. Diabetes - Interactions investigated in the context of Diabetes fig 1A shows interaction of transfected hSIRT3 with COS7 endogenous FOX3O3a for which no seq is available. HCT116 cells, which contain undetectable levels of endogenous SIRT3 by western analysis, were stably transfected with p-myc-hSIRT3-wt. giusd@mail.nih.gov Accepted 2008-DEC-20 by DAVET. 16-Dec-2008: Contacted by JYOTI. Int. J. Biol. Sci. 2008 davet SIRT3 interacts with the daf-16 homolog FOXO3a in the Mitochondria, as well as increases FOXO3a Dependent Gene expression. SIRT3 interacts with the daf-16 homolog FOXO3a in the Mitochondria, as well as increases FOXO3a Dependent Gene expression. Int. J. Biol. Sci. 2008 Jacobs K.M., Pennington J.D., Bisht K.S., Aykin-Burns N., Kim H.S., Mishra M., Sun L., Nguyen P., Ahn B.H., Leclerc J., Deng C.X., Spitz D.R., Gius D. Diabetes - Interactions investigated in the context of Diabetes Apoptosis - Interactions involving proteins with a function related to apoptosis fig 1A shows interaction of transfected hSIRT3 with COS7 endogenous FOX3O3a for which no seq is available. HCT116 cells, which contain undetectable levels of endogenous SIRT3 by western analysis, were stably transfected with p-myc-hSIRT3-wt. giusd@mail.nih.gov 16-Dec-2008: Contacted by JYOTI. davet human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Jacobs K.M., Pennington J.D., Bisht K.S., Aykin-Burns N., Kim H.S., Mishra M., Sun L., Nguyen P., Ahn B.H., Leclerc J., Deng C.X., Spitz D.R., Gius D. Diabetes - Interactions investigated in the context of Diabetes fig 1A shows interaction of transfected hSIRT3 with COS7 endogenous FOX3O3a for which no seq is available. HCT116 cells, which contain undetectable levels of endogenous SIRT3 by western analysis, were stably transfected with p-myc-hSIRT3-wt. giusd@mail.nih.gov Accepted 2008-DEC-20 by DAVET. 16-Dec-2008: Contacted by JYOTI. Int. J. Biol. Sci. 2008 davet sco2_human_gene SCO2_HUMAN_GENE gene gene human Homo sapiens Human sodm_human_gene SODM_HUMAN_GENE/SOD2/Superoxide dismutase [Mn], mitochondrial gene gene human Homo sapiens Human sir3_human NAD-dependent protein deacetylase sirtuin-3, mitochondrial SIRT3 SIR2L3 SIR2-like protein 3 Regulatory protein SIR2 homolog 3 protein protein human Homo sapiens Human MAFWGWRAAAALRLWGRVVERVEAGGGVGPFQACGCRLVLGGRDDVSAGLRGSHGARGEPLDPARPLQRPPRPEVPRAFRRQPRAAAPSFFFSSIKGGRRSISFSVGASSVVGSGGSSDKGKLSLQDVAELIRARACQRVVVMVGAGISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELYPGNYKPNVTHYFLRLLHDKGLLLRLYTQNIDGLERVSGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK DIP protein Q9NTG7 original sequence version: 123 DIP protein Q9NTG7 original sequence version: 80 4BA8BD3AC5FC7901 foxo3_human Forkhead box protein O3 FOXO3 FKHRL1 FOXO3A Forkhead in rhabdomyosarcoma-like 1 AF6q21 protein protein protein human Homo sapiens Human MAEAPASPAPLSPLEVELDPEFEPQSRPRSCTWPLQRPELQASPAKPSGETAADSMIPEEEDDEDDEDGGGRAGSAMAIGGGGGSGTLGSGLLLEDSARVLAPGGQDPGSGPATAAGGLSGGTQALLQPQQPLPPPQPGAAGGSGQPRKCSSRRNAWGNLSYADLITRAIESSPDKRLTLSQIYEWMVRCVPYFKDKGDSNSSAGWKNSIRHNLSLHSRFMRVQNEGTGKSSWWIINPDGGKSGKAPRRRAVSMDNSNKYTKSRGRAAKKKAALQTAPESADDSPSQLSKWPGSPTSRSSDELDAWTDFRSRTNSNASTVSGRLSPIMASTELDEVQDDDAPLSPMLYSSSASLSPSVSKPCTVELPRLTDMAGTMNLNDGLTENLMDDLLDNITLPPSQPSPTGGLMQRSSSFPYTTKGSGLGSPTSSFNSTVFGPSSLNSLRQSPMQTIQENKPATFSSMSHYGNQTLQDLLTSDSLSHSDVMMTQSDPLMSQASTAVSAQNSRRNVMLRNDPMMSFAAQPNQGSLVNQNLLHHQHQTQGALGGSRALSNSVSNMGLSESSSLGSAKHQQQSPVSQSMQTLSDSLSGSSLYSTSANLPVMGHEKFPSDLDLDMFNGSLECDMESIIRSELMDADGLDFNFDSLISTQNVVGLNVGNFTGAKQASSQSWVPG E5B4E830665A9982 DIP protein O43524 original sequence version: 82 E5B4E830665A9982 sirt3a-foxo3 1 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-hct116 Human colon carcinoma HCT116 cells hct116 Human colon carcinoma line HCT116 physical association physical association 1B and 1C A SIRT3 acetylation null mutant did also interact. The mutation site (his248tyr) could not be mapped to the SIRT3 aa sequence. foxo3-sodm 2 4 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait association association 3D foxo3-human 2 3 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role bait bait association association 3D