IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Granzyme A cleaves a mitochondrial complex I protein to initiate caspase-independent cell death. Granzyme A cleaves a mitochondrial complex I protein to initiate caspase-independent cell death. Cell (0092-8674) 2008 imex curation Martinvalet D., Dykxhoorn DM., Ferrini R., Lieberman J. Apoptosis - Interactions involving proteins with a function related to apoptosis lieberman@cbr.med.harvard.edu 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions 3 in vitro In vitro protease assay protease assay anti tag western anti tag western blot anti tag western Martinvalet D., Dykxhoorn DM., Ferrini R., Lieberman J. Apoptosis - Interactions involving proteins with a function related to apoptosis lieberman@cbr.med.harvard.edu Accepted 2008-SEP-13 by DAVET. Cell (0092-8674) 2008 davet Only protein-protein interactions 3 imex curation Granzyme A cleaves a mitochondrial complex I protein to initiate caspase-independent cell death. Granzyme A cleaves a mitochondrial complex I protein to initiate caspase-independent cell death. Cell (0092-8674) 2008 imex curation Martinvalet D., Dykxhoorn DM., Ferrini R., Lieberman J. Apoptosis - Interactions involving proteins with a function related to apoptosis lieberman@cbr.med.harvard.edu 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions 3 human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Martinvalet D., Dykxhoorn DM., Ferrini R., Lieberman J. Apoptosis - Interactions involving proteins with a function related to apoptosis lieberman@cbr.med.harvard.edu Accepted 2008-SEP-13 by DAVET. 27-Aug-2008: Contacted by JYOTI. Cell (0092-8674) 2008 davet Only protein-protein interactions 3 imex curation Granzyme A cleaves a mitochondrial complex I protein to initiate caspase-independent cell death. Granzyme A cleaves a mitochondrial complex I protein to initiate caspase-independent cell death. Cell (0092-8674) 2008 imex curation Martinvalet D., Dykxhoorn DM., Ferrini R., Lieberman J. Apoptosis - Interactions involving proteins with a function related to apoptosis lieberman@cbr.med.harvard.edu 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions 3 human-k_562 Homo sapiens myeloid leukemia cells. k562 Myelogenous leukemia K562 cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Martinvalet D., Dykxhoorn DM., Ferrini R., Lieberman J. Apoptosis - Interactions involving proteins with a function related to apoptosis lieberman@cbr.med.harvard.edu Accepted 2008-SEP-13 by DAVET. 27-Aug-2008: Contacted by JYOTI. Cell (0092-8674) 2008 davet Only protein-protein interactions 3 imex curation nu6m_human NADH-ubiquinone oxidoreductase chain 6 MT-ND6 MTND6 NADH6 ND6 NADH dehydrogenase subunit 6 protein protein human Homo sapiens Human MMYALFLLSVGLVMGFVGFSSKPSPIYGGLVLIVSGVVGCVIILNFGGGYMGLMVFLIYLGGMMVVFGYTTAMAIEEYPEAWGSGVEVLVSVLVGLAMEVGLVLWVKEYDGVVVVVNFNSVGSWMIYEGEGSGLIREDPIGAGALYDYGRWLVVVTGWTLFVGVYIVIEIARGN E50CD9F3FAFC600D ndua9_human NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial NADH-ubiquinone oxidoreductase 39 kDa subunit Complex I-39kD NDUFA9 NDUFS2L protein protein human Homo sapiens Human MAAAAQSRVVRVLSMSRSAITAIATSVCHGPPCRQLHHALMPHGKGGRSSVSGIVATVFGATGFLGRYVVNHLGRMGSQVIIPYRCDKYDIMHLRPMGDLGQLLFLEWDARDKDSIRRVVQHSNVVINLIGRDWETKNFDFEDVFVKIPQAIAQLSKEAGVEKFIHVSHLNANIKSSSRYLRNKAVGEKVVRDAFPEAIIVKPSDIFGREDRFLNSFASMHRFGPIPLGSLGWKTVKQPVYVVDVSKGIVNAVKDPDANGKSFAFVGPSRYLLFHLVKYIFAVAHRLFLPFPLPLFAYRWVARVFEISPFEPWITRDKVERMHITDMKLPHLPGLEDLGIQATPLELKAIEVLRRHRTYRWLSAEIEDVKPAKTVNI DIP protein Q16795 original sequence version: 88 66A1CC7FCE86DD0E ndus3_human NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial NDUFS3 NADH-ubiquinone oxidoreductase 30 kDa subunit Complex I-30kD protein protein human Homo sapiens Human MAAAAVARLWWRGILGASALTRGTGRPSVLLLPVRRESAGADTRPTVRPRNDVAHKQLSAFGEYVAEILPKYVQQVQVSCFNELEVCIHPDGVIPVLTFLRDHTNAQFKSLVDLTAVDVPTRQNRFEIVYNLLSLRFNSRIRVKTYTDELTPIESAVSVFKAANWYEREIWDMFGVFFANHPDLRRILTDYGFEGHPFRKDFPLSGYVELRYDDEVKRVVAEPVELAQEFRKFDLNSPWEAFPVYRQPPESLKLEAGDKKPDAK C058D62779BEF17B C058D62779BEF17B ndub6_human NADH dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 NADH-ubiquinone oxidoreductase B17 subunit Complex I-B17 NDUFB6 protein protein human Homo sapiens Human MTGYTPDEKLRLQQLRELRRRWLKDQELSPREPVLPPQKMGPMEKFWNKFLENKSPWRKMVHGVYKKSIFVFTHVLVPVWIIHYYMKYHVSEKPYGIVEKKSRIFPGDTILETGEVIPPMKEFPDQHH E8F419F5EB154D0B hsp71_human Heat shock 70 kDa protein 1A/1B HSPA1A HSPA1 HSPA1B Heat shock 70 kDa protein 1/2 HSX70 protein protein human Homo sapiens Human MAKAAAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVINDGDKPKVQVSYKGETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDRTGKGERNVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQASLEIDSLFEGIDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD 78F513118C96DE66 hs90a_human Heat shock protein HSP 90-alpha HSP90AA1 HSP90A HSPC1 Heat shock 86 kDa Renal carcinoma antigen NY-REN-38 HSPCA Lipopolysaccharide-associated protein 2 protein protein human Homo sapiens Human MPEETQTQDQPMEEEEVETFAFQAEIAQLMSLIINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKLDSGKELHINLIPNKQDRTLTIVDTGIGMTKADLINNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAYLVAEKVTVITKHNDDEQYAWESSAGGSFTVRTDTGEPMGRGTKVILHLKEDQTEYLEERRIKEIVKKHSQFIGYPITLFVEKERDKEVSDDEAEEKEDKEEEKEKEEKESEDKPEIEDVGSDEEEEKKDGDKKKKKKIKEKYIDQEELNKTKPIWTRNPDDITNEEYGEFYKSLTNDWEDHLAVKHFSVEGQLEFRALLFVPRRAPFDLFENRKKKNNIKLYVRRVFIMDNCEELIPEYLNFIRGVVDSEDLPLNISREMLQQSKILKVIRKNLVKKCLELFTELAEDKENYKKFYEQFSKNIKLGIHEDSQNRKKLSELLRYYTSASGDEMVSLKDYCTRMKENQKHIYYITGETKDQVANSAFVERLRKHGLEVIYMIEPIDEYCVQQLKEFEGKTLVSVTKEGLELPEDEEEKKKQEEKKTKFENLCKIMKDILEKKVEKVVVSNRLVTSPCCIVTSTYGWTANMERIMKAQALRDNSTMGYMAAKKHLEINPDHSIIETLRQKAEADKNDKSVKDLVILLYETALLSSGFSLEDPQTHANRIYRMIKLGLGIDEDDPTADDTSAAVTEEMPPLEGDDDTSRMEEVD DIP protein P07900 original sequence version: 197 DIP protein P07900 original sequence version: 116 DIP protein P07900 original sequence version: 150 DIP protein P07900-1 original sequence version: 158 DIP protein P07900 original sequence version: 207 DIP protein P07900 original sequence version: 192 DIP protein P07900 original sequence version: 156 969F65FCC0BC86FD graa_human Granzyme A Granzyme-1 Cytotoxic T-lymphocyte proteinase 1 Hanukkah factor CTL tryptase Fragmentin-1 GZMA CTLA3 HFSP protein protein human Homo sapiens Human MRNSYRFLASSLSVVVSLLLIPEDVCEKIIGGNEVTPHSRPYMVLLSLDRKTICAGALIAKDWVLTAAHCNLNKRSQVILGAHSITREEPTKQIMLVKKEFPYPCYDPATREGDLKLLQLMEKAKINKYVTILHLPKKGDDVKPGTMCQVAGWGRTHNSASWSDTLREVNITIIDRKVCNDRNHYNFNPVIGMNMVCAGSLRGGRDSCNGDSGSPLLCEGVFRGVTSFGLENKCGDPRGPGVYILLSKKHLNWIIMTIKGAV FD773628BA6F301B gzma-ndufs3-1 1 10 enzyme enzyme neutral component neutral component P12544:p.Ser212Ala Alters kinetics of interaction mutation mutation certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:32:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser212ala' Tue Jun 30 16:51:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser212Ala' ecobb Escherichia coli (strain B / BL21) 6 enzyme target enzyme target substrate substrate neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range protein cleavage protein cleavage Cleavage of NDAFS3 at Lys-56, confirmed by mass spectrometry analysis. rhNDUFS3 was specifically and directly cleaved by human GzmA, Fig. 3A The GZMA (P12544) mutation given in the paper could not be mapped to the given sequence. It has been assumed that the nearest serine to this position (allowing for removal of processed peptides) is the residue which has been mutated. ndufs3-ndufb6-1 3 6 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey association association Overexpressed NDUFS3 and K56A-NDUFS3 were incorporated into complex I since they coimmunoprecipitated with NDUFA9, MTND6, and NDUFB6 using an HA antibody Fig. 3C gzma-hsp71-1 2 10 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey association association Manufacturer shows Ab to be raised against C-terminal of HSP90A but gives no detail on cross-reactivity against HSP90B GzmA interacts with Hsp70 and Hsp90, Fig. 5B