DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Bcl-xL induces Drp1-dependent synapse formation in cultured hippocampal neurons. Bcl-xL induces Drp1-dependent synapse formation in cultured hippocampal neurons. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2008 imex curation Li H., Chen Y., Jones AF., Sanger RH., Collis LP., Flannery R., McNay EC., Yu T., Schwarzenbacher R., Bossy B., Bossy-Wetzel E., Bennett MV., Pypaert M., Hickman JA., Smith PJ., Hardwick JM., Jonas EA. Apoptosis - Interactions involving proteins with a function related to apoptosis Coimmunoprecipitations were carried out on lysates of rat hippocampal neurons. The authors confirmed by email that the BCL-XL construct used was human. mbennett@aecom.yu.edu,elizabeth.jonas@yale.edu 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions rat-hippocampus Rattus norvegicus hippocampal neurons hippocampus Hippocampus [Brain hippocampus] anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Li H., Chen Y., Jones AF., Sanger RH., Collis LP., Flannery R., McNay EC., Yu T., Schwarzenbacher R., Bossy B., Bossy-Wetzel E., Bennett MV., Pypaert M., Hickman JA., Smith PJ., Hardwick JM., Jonas EA. Apoptosis - Interactions involving proteins with a function related to apoptosis Coimmunoprecipitations were carried out on lysates of rat hippocampal neurons. The authors confirmed by email that the BCL-XL construct used was human. mbennett@aecom.yu.edu,elizabeth.jonas@yale.edu Accepted 2008-SEP-6 by DAVET. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2008 davet IMEx Bcl-xL induces Drp1-dependent synapse formation in cultured hippocampal neurons. Bcl-xL induces Drp1-dependent synapse formation in cultured hippocampal neurons. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2008 imex curation Li H., Chen Y., Jones AF., Sanger RH., Collis LP., Flannery R., McNay EC., Yu T., Schwarzenbacher R., Bossy B., Bossy-Wetzel E., Bennett MV., Pypaert M., Hickman JA., Smith PJ., Hardwick JM., Jonas EA. Apoptosis - Interactions involving proteins with a function related to apoptosis Coimmunoprecipitations were carried out on lysates of rat hippocampal neurons. The authors confirmed by email that the BCL-XL construct used was human. mbennett@aecom.yu.edu,elizabeth.jonas@yale.edu 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions in vitro In vitro gtpase assay gtpase assay GTPase gtp hydrolisis gtp hydrolisis GTPase predetermined featur predetermined feature Li H., Chen Y., Jones AF., Sanger RH., Collis LP., Flannery R., McNay EC., Yu T., Schwarzenbacher R., Bossy B., Bossy-Wetzel E., Bennett MV., Pypaert M., Hickman JA., Smith PJ., Hardwick JM., Jonas EA. Apoptosis - Interactions involving proteins with a function related to apoptosis Coimmunoprecipitations were carried out on lysates of rat hippocampal neurons. The authors confirmed by email that the BCL-XL construct used was human. mbennett@aecom.yu.edu,elizabeth.jonas@yale.edu Accepted 2008-SEP-6 by DAVET. 27-Aug-2008: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2008 davet IMEx Bcl-xL induces Drp1-dependent synapse formation in cultured hippocampal neurons. Bcl-xL induces Drp1-dependent synapse formation in cultured hippocampal neurons. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2008 imex curation Li H., Chen Y., Jones AF., Sanger RH., Collis LP., Flannery R., McNay EC., Yu T., Schwarzenbacher R., Bossy B., Bossy-Wetzel E., Bennett MV., Pypaert M., Hickman JA., Smith PJ., Hardwick JM., Jonas EA. Apoptosis - Interactions involving proteins with a function related to apoptosis Coimmunoprecipitations were carried out on lysates of rat hippocampal neurons. The authors confirmed by email that the BCL-XL construct used was human. mbennett@aecom.yu.edu,elizabeth.jonas@yale.edu 27-Aug-2008: Contacted by JYOTI. davet Only protein-protein interactions rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Li H., Chen Y., Jones AF., Sanger RH., Collis LP., Flannery R., McNay EC., Yu T., Schwarzenbacher R., Bossy B., Bossy-Wetzel E., Bennett MV., Pypaert M., Hickman JA., Smith PJ., Hardwick JM., Jonas EA. Apoptosis - Interactions involving proteins with a function related to apoptosis Coimmunoprecipitations were carried out on lysates of rat hippocampal neurons. The authors confirmed by email that the BCL-XL construct used was human. mbennett@aecom.yu.edu,elizabeth.jonas@yale.edu Accepted 2008-SEP-6 by DAVET. 27-Aug-2008: Contacted by JYOTI. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2008 davet IMEx dnm1l_human Dynamin-1-like protein Dynamin-like protein Dnm1p/Vps1p-like protein Dynamin family member proline-rich carboxyl-terminal domain less Dynamin-related protein 1 Dynamin-like protein 4 Dynamin-like protein IV DNM1L DLP1 DRP1 protein protein human Homo sapiens Human MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGIVTRRPLILQLVHVSQEDKRKTTGEENGVEAEEWGKFLHTKNKLYTDFDEIRQEIENETERISGNNKGVSPEPIHLKIFSPNVVNLTLVDLPGMTKVPVGDQPKDIELQIRELILRFISNPNSIILAVTAANTDMATSEALKISREVDPDGRRTLAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVVNRSQLDINNKKSVTDSIRDEYAFLQKKYPSLANRNGTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLITKFATEYCNTIEGTAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIELAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAVSRDKSSKVPSALAPASQEPSPAASAEADGKLIQDSRRETKNVASGGGGVGDGVQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW F9521A376B785B71 F9521A376B785B71 gtp guanosine triphosphate - GTP 5'-GTP GTP Guanosine 5'-triphosphate guanosine 5'-triphosphoric acid guanosine triphosphate GUANOSINE-5'-TRIPHOSPHATE H4gtp guanosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 InChI=1/C10H16N5O14P3/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18)/t3-,5-,6-,9-/m1/s1/f/h14,19-20,22,24H,11H2 q07817-1 Bcl-2-like protein 1 BCLX Apoptosis regulator Bcl-X BCL2L1 BCL2L Bcl-xL protein protein human Homo sapiens Human MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK DIP protein Q07817 original sequence version: 82 DIP protein Q07817 original sequence version: 86 DIP protein Q07817-1 original sequence version: 100 DIP protein Q07817-1 original sequence version: 108 DIP protein Q07817 original sequence version: 131 DIP protein Q07817-1 original sequence version: 110 DIP protein Q07817-1 original sequence version: 135 DIP protein Q07817-1 original sequence version: 131 DIP protein Q07817-1 original sequence version: 172 DIP protein Q07817-1 original sequence version: 174 DIP protein Q07817 original sequence version: 174 DIP protein Q07817-1 original sequence version: 189 DIP protein Q07817-1 original sequence version: 192 DIP protein Q07817 original sequence version: 190 E09D3CDD851AE9BE dnm1l_rat Dynamin-1-like protein Dnm1l Dlp1 Drp1 Dynamin-like protein protein protein rat Rattus norvegicus (Rat) Rat MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGVVTRRPLILQLVHVSPEDKRKTTGEENDPATWKNSRHLSKGVEAEEWGKFLHTKNKLYTDFDEIRQEIENETERISGNNKGVSPEPIHLKVFSPNVVNLTLVDLPGMTKVPVGDQPKDIELQIRELILRFISNPNSIILAVTAANTDMATSEALKISREVDPDGRRTLAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVVNRSQLDINNKKSVTDSIRDEYAFLQKKYPSLANRNGTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLITKFATEYCNTIEGTAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIELAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAVSRDKSSKVPSALAPASQEPSPAASAEADGKLIQDNRRETKNVASAGGGIGDGGRIGDGGQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW DIP protein O35303 original sequence version: 51 0568353907794C43 p53563-1 Bcl-2-like protein 1 Bcl-xL Bcl2l1 Bclx Blc2l Apoptosis regulator Bcl-X protein protein rat Rattus norvegicus (Rat) Rat MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEETEPERETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIASWMATYLNDHLEPWIQENGGWDTFVDLYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK DIP protein P53563-1 original sequence version: 81 DIP protein P53563 original sequence version: 135 2B62B6C63864BC8F b2cl1_human Bcl-2-like protein 1 BCL2L1 BCL2L BCLX Apoptosis regulator Bcl-X protein protein human Homo sapiens Human MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK E09D3CDD851AE9BE bcl2l1-dnm1l-3 1 6 unspecified role unspecified role bait bait n-terminal gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey physical association physical association 6 A Immunoprecipitation of GFP-Bcl-xL with anti-GFP antibody coprecipitated endogenous Drp1 Q07817-1 transduction bcl2l1-dnm1l-1 3 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 6 B Anti-Drp1 antibody coprecipitated endogenous Bcl-xL protein from brain lysate, and conversely, anti-Bcl-xL antibody coprecipitated endogenous brain Drp1 bcl2l1-dnm1l-2 3 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 6 B Anti-Drp1 antibody coprecipitated endogenous Bcl-xL protein from brain lysate, and conversely, anti-Bcl-xL antibody coprecipitated endogenous brain Drp1 dnml1-gtp 2 5 enzyme target enzyme target substrate substrate unspecified role unspecified role 9 stimulator stimulator unspecified role unspecified role 4 enzyme enzyme unspecified role unspecified role gtpase reaction gtpase reaction 6 C Bcl-xL, which had little GTPase activity itself, increased Drp1 GTPase activity by 60%, relative to a control protein BSA Bcl-xL increased Drp1 GTPase activity by about 60%, relative to the control protein, BSA.