IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mps1 phosphorylates Borealin to control Aurora B activity and chromosome alignment. Mps1 phosphorylates Borealin to control Aurora B activity and chromosome alignment. Cell (0092-8674) 2008 imex curation Jelluma N., Brenkman AB., van den Broek NJ., Cruijsen CW., van Osch MH., Lens SM., Medema RH., Kops GJ. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of TTK/MPS1 taken from PMID: 11927556 and mutation mapping. Source of Borealin identified from mapping of mass spec peptides. g.j.p.l.kops@umcutrecht.nl 14-May-2008: Contacted by JYOTI. Only protein-protein interactions 3 human-u2-os Human U2-OS osteosarcoma cell line u2-os U2-OS osteocaroma cell line Not in CAPRI and no primary literature reference pull down pull down western blot western blot Immuno blot Immuno blot Jelluma N., Brenkman AB., van den Broek NJ., Cruijsen CW., van Osch MH., Lens SM., Medema RH., Kops GJ. Apoptosis - Interactions involving proteins with a function related to apoptosis Cells treated with Nocodazole for 16 hr. Immunoprecipitates isolated following pulldown of LAP-Borealin using S-protein-Agarose. Source of TTK/MPS1 taken from PMID: 11927556 and mutation mapping. Source of Borealin identified from mapping of mass spec peptides. g.j.p.l.kops@umcutrecht.nl Accepted 2008-MAY-16 by CDEROW. 14-May-2008: Contacted by JYOTI. Cell (0092-8674) 2008 Only protein-protein interactions 3 imex curation Mps1 phosphorylates Borealin to control Aurora B activity and chromosome alignment. Mps1 phosphorylates Borealin to control Aurora B activity and chromosome alignment. Cell (0092-8674) 2008 imex curation Jelluma N., Brenkman AB., van den Broek NJ., Cruijsen CW., van Osch MH., Lens SM., Medema RH., Kops GJ. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of TTK/MPS1 taken from PMID: 11927556 and mutation mapping. Source of Borealin identified from mapping of mass spec peptides. g.j.p.l.kops@umcutrecht.nl 14-May-2008: Contacted by JYOTI. Only protein-protein interactions 3 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Jelluma N., Brenkman AB., van den Broek NJ., Cruijsen CW., van Osch MH., Lens SM., Medema RH., Kops GJ. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of TTK/MPS1 taken from PMID: 11927556 and mutation mapping. Source of Borealin identified from mapping of mass spec peptides. g.j.p.l.kops@umcutrecht.nl Accepted 2008-MAY-16 by CDEROW. 14-May-2008: Contacted by JYOTI. Cell (0092-8674) 2008 Only protein-protein interactions 3 imex curation birc5_human Baculoviral IAP repeat-containing protein 5 Apoptosis inhibitor survivin Apoptosis inhibitor 4 BIRC5 API4 IAP4 protein protein human Homo sapiens Human MGAPTLPPAWQPFLKDHRISTFKNWPFLEGCACTPERMAEAGFIHCPTENEPDLAQCFFCFKELEGWEPDDDPIEEHKKHSSGCAFLSVKKQFEELTLGEFLKLDRERAKNKIAKETNNKKKEFEETAKKVRRAIEQLAAMD DIP protein O15392 original sequence version: 129 DIP protein O15392 original sequence version: 132 DIP protein O15392-1 original sequence version: 141 DIP protein O15392-1 original sequence version: 143 9E7CADCDF2822286 ttk_human Dual specificity protein kinase TTK TTK MPS1L1 Phosphotyrosine picked threonine-protein kinase MPS1 protein protein human Homo sapiens Human MESEDLSGRELTIDSIMNKVRDIKNKFKNEDLTDELSLNKISADTTDNSGTVNQIMMMANNPEDWLSLLLKLEKNSVPLSDALLNKLIGRYSQAIEALPPDKYGQNESFARIQVRFAELKAIQEPDDARDYFQMARANCKKFAFVHISFAQFELSQGNVKKSKQLLQKAVERGAVPLEMLEIALRNLNLQKKQLLSEEEKKNLSASTVLTAQESFSGSLGHLQNRNNSCDSRGQTTKARFLYGENMPPQDAEIGYRNSLRQTNKTKQSCPFGRVPVNLLNSPDCDVKTDDSVVPCFMKRQTSRSECRDLVVPGSKPSGNDSCELRNLKSVQNSHFKEPLVSDEKSSELIITDSITLKNKTESSLLAKLEETKEYQEPEVPESNQKQWQSKRKSECINQNPAASSNHWQIPELARKVNTEQKHTTFEQPVFSVSKQSPPISTSKWFDPKSICKTPSSNTLDDYMSCFRTPVVKNDFPPACQLSTPYGQPACFQQQQHQILATPLQNLQVLASSSANECISVKGRIYSILKQIGSGGSSKVFQVLNEKKQIYAIKYVNLEEADNQTLDSYRNEIAYLNKLQQHSDKIIRLYDYEITDQYIYMVMECGNIDLNSWLKKKKSIDPWERKSYWKNMLEAVHTIHQHGIVHSDLKPANFLIVDGMLKLIDFGIANQMQPDTTSVVKDSQVGTVNYMPPEAIKDMSSSRENGKSKSKISPKSDVWSLGCILYYMTYGKTPFQQIINQISKLHAIIDPNHEIEFPDIPEKDLQDVLKCCLKRDPKQRISIPELLAHPYVQIQTHPVNQMAKGTTEEMKYVLGQLVGLNSPNSILKAAKTLYEHYSGGESHNSSSSKTFEKKRGKK 51F40A3CD1677AC5 borea_human Borealin Dasra-B Cell division cycle-associated protein 8 Pluripotent embryonic stem cell-related gene 3 protein CDCA8 PESCRG3 protein protein human Homo sapiens Human MAPRKGSSRVAKTNSLRRRKLASFLKDFDREVEIRIKQIESDRQNLLKEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEINKLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPPSKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVFKTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQRHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK DIP protein Q53HL2 original sequence version: 57 519978A7C295C571 aurkb_human Aurora kinase B AIRK2 Aurora- and IPL1-like midbody-associated protein 1 Aurora/IPL1-related kinase 2 STK-1 Serine/threonine-protein kinase 12 Serine/threonine-protein kinase 5 STK1 STK5 Aurora 1 AURKB AIK2 AIM1 ARK2 STK12 Serine/threonine-protein kinase aurora-B protein protein human Homo sapiens Human MAQKENSYPWPYGRQTAPSGLSTLPQRVLRKEPVTPSALVLMSRSNVQPTAAPGQKVMENSSGTPDILTRHFTIDDFEIGRPLGKGKFGNVYLAREKKSHFIVALKVLFKSQIEKEGVEHQLRREIEIQAHLHHPNILRLYNYFYDRRRIYLILEYAPRGELYKELQKSCTFDEQRTATIMEELADALMYCHGKKVIHRDIKPENLLLGLKGELKIADFGWSVHAPSLRRKTMCGTLDYLPPEMIEGRMHNEKVDLWCIGVLCYELLVGNPPFESASHNETYRRIVKVDLKFPASVPMGAQDLISKLLRHNPSERLPLAQVSAHPWVRANSRRVLPPSALQSVA DIP protein Q96GD4 original sequence version: 88 DIP protein Q96GD4 original sequence version: 113 DIP protein Q96GD4 original sequence version: 116 DIP protein Q96GD4 original sequence version: 122 DIP protein Q96GD4 original sequence version: 177 DIP protein Q96GD4-1 original sequence version: 177 A5ED13EF5A1FAFBF cdca8-ttk-1 2 4 enzyme enzyme neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P33981:p.Asp664Ala mutation mutation certain certain sequence position certain certain certain sequence position certain D A Wed May 02 16:38:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp664ala' Tue Jun 30 17:25:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp664Ala' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 5 enzyme target enzyme target substrate substrate neutral component neutral component region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecobb Escherichia coli (strain B / BL21) phosphorylation phosphorylation reaction phosphorylation Phosphorylation of CDCA8. Subsequent mass spec and mutagenesis studies show Thr-88, Thr-94, Thr-169 and Thr-230 of CDCA8 to be phosphorylated by TTK Phosphorylation of Borealin by TTK/Mps1 Figs. 6A, 6B Supp. Fig. 4 Asp664Ala is catalytically inactive cdca8-aurkb-1 1 5 unspecified role unspecified role bait bait lap tagged region tag tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey association association Figs. 6D Q53HL2 transfection ttk-1 2 4 putative self putative self putative self putative self P33981:p.Asp664Ala mutation mutation certain certain sequence position certain certain certain sequence position certain D A Wed May 02 20:01:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp664ala' Tue Jun 30 17:11:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp664Ala' region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. phosphorylation phosphorylation reaction phosphorylation true Autophosphorylation of TTK (Mps1). Kinase activity of anti-GFP immunoprecipitates of cells transfected with empty vector (Con) or RNAi-resistant (indicated by asteriks) LAP-tagged wild-type (WT*) or kinase-dead (KD*) Mps1. Figs. 1B Autophosphorylation not present in kinase-dead asp664ala mutant.