IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Acetylcholinesterase-R increases germ cell apoptosis but enhances sperm motility. Acetylcholinesterase-R increases germ cell apoptosis but enhances sperm motility. J. Cell. Mol. Med. (1582-1838) 2008 Mor I., Sklan EH., Podoly E., Pick M., Kirschner M., Yogev L., Bar-Sheshet Itach S., Schreiber L., Geyer B., Mor T., Grisaru D., Soreq H. Apoptosis - Interactions involving proteins with a function related to apoptosis Synapse - Interactions of proteins with an established role in the presynapse. A UniProtKB entry corresponding to the form of ACHE referred to as AChE-R by the authors could not be found. Thus the parent sequence for the ACHE entry has been used. soreq@cc.huji.ac.il 27-Oct-2007: Contacted by JYOTI. An adult human testis cDNA library was used. orchard mouse-testis Mus musculus testis testis anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mor I., Sklan EH., Podoly E., Pick M., Kirschner M., Yogev L., Bar-Sheshet Itach S., Schreiber L., Geyer B., Mor T., Grisaru D., Soreq H. Apoptosis - Interactions involving proteins with a function related to apoptosis A UniProtKB entry corresponding to the form of ACHE referred to as AChE-R by the authors could not be found. Thus the parent sequence for the ACHE entry has been used. soreq@cc.huji.ac.il Accepted 2008-JUN-20 by ORCHARD. 27-Oct-2007: Contacted by JYOTI. An adult human testis cDNA library was used. J. Cell. Mol. Med. (1582-1838) 2008 orchard Acetylcholinesterase-R increases germ cell apoptosis but enhances sperm motility. Acetylcholinesterase-R increases germ cell apoptosis but enhances sperm motility. J. Cell. Mol. Med. (1582-1838) 2008 Mor I., Sklan EH., Podoly E., Pick M., Kirschner M., Yogev L., Bar-Sheshet Itach S., Schreiber L., Geyer B., Mor T., Grisaru D., Soreq H. Apoptosis - Interactions involving proteins with a function related to apoptosis Synapse - Interactions of proteins with an established role in the presynapse. A UniProtKB entry corresponding to the form of ACHE referred to as AChE-R by the authors could not be found. Thus the parent sequence for the ACHE entry has been used. soreq@cc.huji.ac.il 27-Oct-2007: Contacted by JYOTI. An adult human testis cDNA library was used. orchard yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Mor I., Sklan EH., Podoly E., Pick M., Kirschner M., Yogev L., Bar-Sheshet Itach S., Schreiber L., Geyer B., Mor T., Grisaru D., Soreq H. Apoptosis - Interactions involving proteins with a function related to apoptosis A UniProtKB entry corresponding to the form of ACHE referred to as AChE-R by the authors could not be found. Thus the parent sequence for the ACHE entry has been used. soreq@cc.huji.ac.il Accepted 2008-JUN-20 by ORCHARD. An adult human testis cDNA library was used. J. Cell. Mol. Med. (1582-1838) 2008 orchard Acetylcholinesterase-R increases germ cell apoptosis but enhances sperm motility. Acetylcholinesterase-R increases germ cell apoptosis but enhances sperm motility. J. Cell. Mol. Med. (1582-1838) 2008 Mor I., Sklan EH., Podoly E., Pick M., Kirschner M., Yogev L., Bar-Sheshet Itach S., Schreiber L., Geyer B., Mor T., Grisaru D., Soreq H. Apoptosis - Interactions involving proteins with a function related to apoptosis Synapse - Interactions of proteins with an established role in the presynapse. A UniProtKB entry corresponding to the form of ACHE referred to as AChE-R by the authors could not be found. Thus the parent sequence for the ACHE entry has been used. soreq@cc.huji.ac.il 27-Oct-2007: Contacted by JYOTI. An adult human testis cDNA library was used. orchard in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Mor I., Sklan EH., Podoly E., Pick M., Kirschner M., Yogev L., Bar-Sheshet Itach S., Schreiber L., Geyer B., Mor T., Grisaru D., Soreq H. Apoptosis - Interactions involving proteins with a function related to apoptosis A UniProtKB entry corresponding to the form of ACHE referred to as AChE-R by the authors could not be found. Thus the parent sequence for the ACHE entry has been used. A UniProtKB entry corresponding to the form of ACHE referred to as AChE-R by the authors could not be found. Thus the parent sequence for the ACHE entry has been used. A UniProtKB entry corresponding to the form of ACHE referred to as AChE-R by the authors could not be found. Thus the parent sequence for the ACHE entry has been used. soreq@cc.huji.ac.il Accepted 2008-JUN-20 by ORCHARD. 27-Oct-2007: Contacted by JYOTI. An adult human testis cDNA library was used. J. Cell. Mol. Med. (1582-1838) 2008 orchard enoa_human Alpha-enolase ENO1 ENO1L1 MBPB1 MPB1 2-phospho-D-glycerate hydro-lyase Non-neural enolase Enolase 1 Phosphopyruvate hydratase C-myc promoter-binding protein MBP-1 MPB-1 Plasminogen-binding protein protein protein human Homo sapiens Human MSILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGVSKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVIGMDVAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKAKFAGRNFRNPLAK DIP protein P06733 original sequence version: null A0ED663FCC15ADA5 enoa_mouse Alpha-enolase Eno1 Eno-1 2-phospho-D-glycerate hydro-lyase Non-neural enolase Enolase 1 protein protein mouse Mus musculus Mouse MSILRIHAREIFDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDNDKTRFMGKGVSQAVEHINKTIAPALVSKKVNVVEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNPEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEALELLKTAIAKAGYTDQVVIGMDVAASEFYRSGKYDLDFKSPDDPSRYITPDQLADLYKSFVQNYPVVSIEDPFDQDDWGAWQKFTASAGIQVVGDDLTVTNPKRIAKAASEKSCNCLLLKVNQIGSVTESLQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQILRIEEELGSKAKFAGRSFRNPLAK DBEF6270A70DE3A6 2-phospho-d-glyceric acid 2-phospho-D-glyceric acid small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) rack1_human Receptor of activated protein C kinase 1 Small ribosomal subunit protein RACK1 HLC7 PIG21 RACK1 Cell proliferation-inducing gene 21 protein GNB2L1 Guanine nucleotide-binding protein subunit beta-2-like 1 Guanine nucleotide-binding protein subunit beta-like protein 12.3 Human lung cancer oncogene 7 protein Receptor for activated C kinase protein protein human Homo sapiens Human MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR DIP protein P63244 original sequence version: 71 257F91E369ED2044 q9jjp2-1 Tumor protein p73 Tp73 P73 Trp73 p53-like transcription factor p53-related protein Alpha protein protein mouse Mus musculus Mouse MAQTSSSSSSTFEHLWSSLEPDSTYFDLPQPSQGTSEASGSEESNMDVFHLQGMAQFNLLSSAMDQMGSRAAPASPYTPEHAASAPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCPIQIKVSTPPPPGTAIRAMPVYKKAEHVTDIVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLAQYVDDPVTGRQSVVVPYEPPQVGTEFTTILYNFMCNSSCVGGMNRRPILVIITLETRDGQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESTTKNGAASKRAFKQSPPAIPALGTNVKKRRHGDEDMFYMHVRGRENFEILMKVKESLELMELVPQPLVDSYRQQQQQQLLQRPSHLQPPSYGPVLSPMNKVHGGVNKLPSVNQLVGQPPPHSSAAGPNLGPMGSGMLNSHGHSMPANGEMNGGHSSQTMVSGSHCTPPPPYHADPSLVSFLTGLGCPNCIECFTSQGLQSIYHLQNLTIEDLGALKVPDQYRMTIWRGLQDLKQSHDCGQQLLRSSSNAATISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGAGAVTGPDEWADFGFDLPDCKSRKQPIKEEFTETESH E364D566A90CBF1D q9jjp2-2 Tumor protein p73 Tp73 P73 Trp73 p53-like transcription factor p53-related protein dN-Alpha protein protein mouse Mus musculus Mouse MLYVGDPMRHLATAQFNLLSSAMDQMGSRAAPASPYTPEHAASAPTHSPYAQPSSTFDTMSPAPVIPSNTDYPGPHHFEVTFQQSSTAKSATWTYSPLLKKLYCQIAKTCPIQIKVSTPPPPGTAIRAMPVYKKAEHVTDIVKRCPNHELGRDFNEGQSAPASHLIRVEGNNLAQYVDDPVTGRQSVVVPYEPPQVGTEFTTILYNFMCNSSCVGGMNRRPILVIITLETRDGQVLGRRSFEGRICACPGRDRKADEDHYREQQALNESTTKNGAASKRAFKQSPPAIPALGTNVKKRRHGDEDMFYMHVRGRENFEILMKVKESLELMELVPQPLVDSYRQQQQQQLLQRPSHLQPPSYGPVLSPMNKVHGGVNKLPSVNQLVGQPPPHSSAAGPNLGPMGSGMLNSHGHSMPANGEMNGGHSSQTMVSGSHCTPPPPYHADPSLVSFLTGLGCPNCIECFTSQGLQSIYHLQNLTIEDLGALKVPDQYRMTIWRGLQDLKQSHDCGQQLLRSSSNAATISIGGSGELQRQRVMEAVHFRVRHTITIPNRGGAGAVTGPDEWADFGFDLPDCKSRKQPIKEEFTETESH Produced by alternative promoter usage. 8580C7EFA21B5797 aces_human Acetylcholinesterase ACHE protein protein human Homo sapiens Human MRPPQCLLHTPSLASPLLLLLLWLLGGGVGAEGREDAELLVTVRGGRLRGIRLKTPGGPVSAFLGIPFAEPPMGPRRFLPPEPKQPWSGVVDATTFQSVCYQYVDTLYPGFEGTEMWNPNRELSEDCLYLNVWTPYPRPTSPTPVLVWIYGGGFYSGASSLDVYDGRFLVQAERTVLVSMNYRVGAFGFLALPGSREAPGNVGLLDQRLALQWVQENVAAFGGDPTSVTLFGESAGAASVGMHLLSPPSRGLFHRAVLQSGAPNGPWATVGMGEARRRATQLAHLVGCPPGGTGGNDTELVACLRTRPAQVLVNHEWHVLPQESVFRFSFVPVVDGDFLSDTPEALINAGDFHGLQVLVGVVKDEGSYFLVYGAPGFSKDNESLISRAEFLAGVRVGVPQVSDLAAEAVVLHYTDWLHPEDPARLREALSDVVGDHNVVCPVAQLAGRLAAQGARVYAYVFEHRASTLSWPLWMGVPHGYEIEFIFGIPLDPSRNYTAEEKIFAQRLMRYWANFARTGDPNEPRDPKAPQWPPYTAGAQQYVSLDLRPLEVRRGLRAQACAFWNRFLPKLLSATDTLDEAERQWKAEFHRWSSYMVHWKNQFDHYSKQDRCSDL B9AA84C77831C302 rack1_mouse Receptor of activated protein C kinase 1 p205 Receptor of activated protein kinase C 1 12-3 Gnb2-rs1 Gnb2l1 Guanine nucleotide-binding protein subunit beta-2-like 1 Rack1 Receptor for activated C kinase protein protein mouse Mus musculus Mouse MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWNTLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTLDGGDIINALCFSPNRYWLCAATGPSIKIWDLEGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQTLFAGYTDNLVRVWQVTIGTR 257F91E369ED2044 human_arp26_peptide ARP26 peptide based on the C-terminal sequence of the AChE-R isoform of human acetylcholinesterase peptide peptide polypeptide oligopeptide oligopeptide polypeptide human Homo sapiens Human GMQGPAGSGWEEGSGSPPGVTPLFSP An amino acid sequence which is not represented in UniProt 965EE35DD1C5143A acetylcholine Acetylcholine small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ache-gnb2l1 2 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain c-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association A cDNA expression library from adult human testis (3.5 106 clones) was screened with the C-terminal region of human AChE-R (ARP-53) as bait [5] (Fig. 1A). This yielded 13 independent clones reflecting partner protein interactions. Four of the identified clones included RACK1 1 A, B ache-eno1-1 2 4 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role bait bait c-terminal sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association A cDNA expression library from adult human testis (3.5 106 clones) was screened with the C-terminal region of human AChE-R (ARP-53) as bait [5] (Fig. 1A). This yielded 13 independent clones reflecting partner protein interactions. Four of the identified clones included RACK1 sequences spanning most of the coding sequence, including WD domains 5�6 shown to interact with AChE-R in neurons [5] (Fig. 1B). A single enolase- encoding clone covered 49% of the coding region, spanning its active site 1 A, B ache-acetylcholine 3 13 enzyme target enzyme target substrate substrate unspecified role unspecified role 10 enzyme enzyme unspecified role unspecified role nicbe Nicotiana benthamiana 4 stimulator stimulator unspecified role unspecified role enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction 1 C This elevated AChE-Rs cholinesterase activity Incubation with enolase-alpha increased ACHE's acetylcholinesterase activity. Km = 4.6 E-5 M; Km (in presence of enolase-alpha = 5.5 E-5. Vmax = 12.6 arbitrary units/min; Vmax (in presence of enolase-alpha) = 15 arbitrary units/min. ache-gnb2l1-2 1 10 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey physical association physical association 1 D Antibodies to the AChE common domain, but not nonspecific rabbit immunoglobulins, co-precipitated a 36KD protein recognized by antibodies to RACK1 This interaction occurred in testicular homogenates from TgR mice, which overexpress human ACHE (hAChE-R). P22303 overexpressed eno1-ache 1 5 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association 1 D antibodies to nonneuronal enolase co-precipitated 60 and 66KD proteins, recognized by the anti-AChE antibodies P22303 overexpressed This interaction occurred in testicular homogenates from TgR mice, which overexpress human ACHE (hAChE-R). gnb2l1-trp53 1 8 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait association association 3 D RACK1 precipitates from TgR testes pulled down less TAp73 than control homogenates (RACK1 i.p., Fig. 3D), suggesting larger amounts of TAp73 remained free. Supporting this notion, TgR homogenates showed elevated levels of Np73, known to be induced by TAp73, which also co-precipitated with RACK1 eno1-2_phospho_d 3 10 stimulator stimulator neutral component neutral component 4 enzyme enzyme unspecified role unspecified role 6 enzyme target enzyme target substrate substrate unspecified role unspecified role 12 stimulator stimulator neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction 4 B The enolase-alpha activity of enolase-alpha was stimulated by the peptide ARP26 (GMQGPAGSGWEEGSGSPPGVTPLFSP), which is at the C-terminus of the form of ACHE referred to by the authors as AChE-R. The enolase-alpha activity of enolase-alpha was not stimulated by the peptide ASP23 (WSSYMVHWKQFDHYSKQDRCSDL), which is at the C-terminus of the form of ACHE referred to by the authors as AChE-S. ache-acetylcholine-1 3 13 enzyme target enzyme target substrate substrate unspecified role unspecified role 10 enzyme enzyme unspecified role unspecified role nicbe Nicotiana benthamiana 7 stimulator stimulator unspecified role unspecified role enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction 1 C This elevated AChE-Rs cholinesterase activity Incubation with GNB2L1 increased ACHE's acetylcholinesterase activity. Km = 4.6 E-5 M; Km (in presence of GNB2L1) = 4.8 E-5 M. Vmax = 12.6 arbitrary units/min; Vmax (in presence of GNB2L1) = 15 arbitrary units/min. ache-acetylcholine-2 3 13 enzyme target enzyme target substrate substrate unspecified role unspecified role 10 enzyme enzyme unspecified role unspecified role nicbe Nicotiana benthamiana enzymatic reaction enzymatic reaction Biochemical reaction Biochemical reaction 1 C This elevated AChE-Rs cholinesterase activity Km = 4.6 E-5 M. Vmax = 12.6 arbitrary units/min.