IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ IAP Antagonists Induce Autoubiquitination of c-IAPs, NF-kappaB Activation, and TNFalpha-Dependent Apoptosis. IAP Antagonists Induce Autoubiquitination of c-IAPs, NF-kappaB Activation, and TNFalpha-Dependent Apoptosis. Cell (0092-8674) 2007 imex curation Varfolomeev E., Blankenship JW., Wayson SM., Fedorova AV., Kayagaki N., Garg P., Zobel K., Dynek JN., Elliott LO., Wallweber HJ., Flygare JA., Fairbrother WJ., Deshayes K., Dixit VM., Vucic D. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of c-IAP1/Birc2 taken from PMID:11090634, c-IAP2/Birc3 from PMID: 11801603, X-IAP/Birc4 from PMID: 15485396 domagoj@gene.com 16-April-2008: Contacted by JYOTI. Only protein-protein interactions 2 in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Varfolomeev E., Blankenship JW., Wayson SM., Fedorova AV., Kayagaki N., Garg P., Zobel K., Dynek JN., Elliott LO., Wallweber HJ., Flygare JA., Fairbrother WJ., Deshayes K., Dixit VM., Vucic D. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of c-IAP1/Birc2 taken from PMID:11090634, c-IAP2/Birc3 from PMID: 11801603, X-IAP/Birc4 from PMID: 15485396 domagoj@gene.com Accepted 2008-APR-17 by CDEROW. 16-April-2008: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 2 imex curation IAP Antagonists Induce Autoubiquitination of c-IAPs, NF-kappaB Activation, and TNFalpha-Dependent Apoptosis. IAP Antagonists Induce Autoubiquitination of c-IAPs, NF-kappaB Activation, and TNFalpha-Dependent Apoptosis. Cell (0092-8674) 2007 imex curation Varfolomeev E., Blankenship JW., Wayson SM., Fedorova AV., Kayagaki N., Garg P., Zobel K., Dynek JN., Elliott LO., Wallweber HJ., Flygare JA., Fairbrother WJ., Deshayes K., Dixit VM., Vucic D. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of c-IAP1/Birc2 taken from PMID:11090634, c-IAP2/Birc3 from PMID: 11801603, X-IAP/Birc4 from PMID: 15485396 domagoj@gene.com 16-April-2008: Contacted by JYOTI. Only protein-protein interactions 2 in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Varfolomeev E., Blankenship JW., Wayson SM., Fedorova AV., Kayagaki N., Garg P., Zobel K., Dynek JN., Elliott LO., Wallweber HJ., Flygare JA., Fairbrother WJ., Deshayes K., Dixit VM., Vucic D. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of c-IAP1/Birc2 taken from PMID:11090634, c-IAP2/Birc3 from PMID: 11801603, X-IAP/Birc4 from PMID: 15485396 domagoj@gene.com Accepted 2008-APR-17 by CDEROW. Cell (0092-8674) 2007 Only protein-protein interactions 2 imex curation IAP Antagonists Induce Autoubiquitination of c-IAPs, NF-kappaB Activation, and TNFalpha-Dependent Apoptosis. IAP Antagonists Induce Autoubiquitination of c-IAPs, NF-kappaB Activation, and TNFalpha-Dependent Apoptosis. Cell (0092-8674) 2007 imex curation Varfolomeev E., Blankenship JW., Wayson SM., Fedorova AV., Kayagaki N., Garg P., Zobel K., Dynek JN., Elliott LO., Wallweber HJ., Flygare JA., Fairbrother WJ., Deshayes K., Dixit VM., Vucic D. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of c-IAP1/Birc2 taken from PMID:11090634, c-IAP2/Birc3 from PMID: 11801603, X-IAP/Birc4 from PMID: 15485396 domagoj@gene.com 16-April-2008: Contacted by JYOTI. Only protein-protein interactions 2 in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Varfolomeev E., Blankenship JW., Wayson SM., Fedorova AV., Kayagaki N., Garg P., Zobel K., Dynek JN., Elliott LO., Wallweber HJ., Flygare JA., Fairbrother WJ., Deshayes K., Dixit VM., Vucic D. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of c-IAP1/Birc2 taken from PMID:11090634, c-IAP2/Birc3 from PMID: 11801603, X-IAP/Birc4 from PMID: 15485396 domagoj@gene.com Accepted 2008-APR-17 by CDEROW. 16-April-2008: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 2 imex curation IAP Antagonists Induce Autoubiquitination of c-IAPs, NF-kappaB Activation, and TNFalpha-Dependent Apoptosis. IAP Antagonists Induce Autoubiquitination of c-IAPs, NF-kappaB Activation, and TNFalpha-Dependent Apoptosis. Cell (0092-8674) 2007 imex curation Varfolomeev E., Blankenship JW., Wayson SM., Fedorova AV., Kayagaki N., Garg P., Zobel K., Dynek JN., Elliott LO., Wallweber HJ., Flygare JA., Fairbrother WJ., Deshayes K., Dixit VM., Vucic D. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of c-IAP1/Birc2 taken from PMID:11090634, c-IAP2/Birc3 from PMID: 11801603, X-IAP/Birc4 from PMID: 15485396 domagoj@gene.com 16-April-2008: Contacted by JYOTI. Only protein-protein interactions 2 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Varfolomeev E., Blankenship JW., Wayson SM., Fedorova AV., Kayagaki N., Garg P., Zobel K., Dynek JN., Elliott LO., Wallweber HJ., Flygare JA., Fairbrother WJ., Deshayes K., Dixit VM., Vucic D. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of c-IAP1/Birc2 taken from PMID:11090634, c-IAP2/Birc3 from PMID: 11801603, X-IAP/Birc4 from PMID: 15485396 domagoj@gene.com Accepted 2008-APR-17 by CDEROW. 16-April-2008: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 2 imex curation methyl 1-{(2s)-2-cyclohexyl-2-[(n-methyl-l-alanyl)amino]acetyl}-l-prolyl-beta-phenyl-l-phenylalaninate Monovalent IAP antagonist 1-{(2S)-2-cyclohexyl-2-[(N-methyl-L-alanyl)amino]acetyl}-L-prolyl-beta-phenyl-L-phenylalaninate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) casp9_human Caspase-9 ICE-like apoptotic protease 6 Apoptotic protease Mch-6 Apoptotic protease-activating factor 3 CASP9 MCH6 protein protein human Homo sapiens Human MDEADRRLLRRCRLRLVEELQVDQLWDALLSRELFRPHMIEDIQRAGSGSRRDQARQLIIDLETRGSQALPLFISCLEDTGQDMLASFLRTNRQAAKLSKPTLENLTPVVLRPEIRKPEVLRPETPRPVDIGSGGFGDVGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRTRTGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELAQQDHGALDCCVVVILSHGCQASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEVASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRDPKSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMPGCFNFLRKKLFFKTS DIP protein P55211 original sequence version: 174 DIP protein P55211 original sequence version: 167 DIP protein P55211 original sequence version: 124 DIP protein P55211 original sequence version: 125 DIP protein P55211 original sequence version: 123 DIP protein P55211 original sequence version: 153 78E0180DF2A3BDD2 n,n'-(hexane-1,6-diyl)bis(1-{(2s)-2-cyclohexyl-2-[(n-methyl-l-alanyl)amino]acetyl}-l-prolyl-beta-phenyl-l-phenylalaninamide) Bivalent IAP antagonist N,N'-hexane-1,6-diylbis[1-{(2S)-2-cyclohexyl-2-[(N-methyl-L-alanyl)amino]acetyl}-L-prolyl-beta-phenyl-L-phenylalaninamide] small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) xiap_human E3 ubiquitin-protein ligase XIAP Inhibitor of apoptosis protein 3 X-linked inhibitor of apoptosis protein IAP-like protein XIAP API3 IAP3 BIRC4 Baculoviral IAP repeat-containing protein 4 RING-type E3 ubiquitin transferase XIAP protein protein human Homo sapiens Human MTFNSFEGSKTCVPADINKEEEFVEEFNRLKTFANFPSGSPVSASTLARAGFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLENSATQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDISDTIYPRNPAMYSEEARLKSFQNWPDYAHLTPRELASAGLYYTGIGDQVQCFCCGGKLKNWEPCDRAWSEHRRHFPNCFFVLGRNLNIRSESDAVSSDRNFPNSTNLPRNPSMADYEARIFTFGTWIYSVNKEQLARAGFYALGEGDKVKCFHCGGGLTDWKPSEDPWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEECLVRTTEKTPSLTRRIDDTIFQNPMVQEAIRMGFSFKDIKKIMEEKIQISGSNYKSLEVLVADLVNAQKDSMQDESSQTSLQKEISTEEQLRRLQEEKLCKICMDRNIAIVFVPCGHLVTCKQCAEAVDKCPMCYTVITFKQKIFMS DIP protein P98170 original sequence version: 168 DIP protein P98170 original sequence version: 118 DIP protein P98170 original sequence version: 153 9D394C16D45EB635 m3k14_human Mitogen-activated protein kinase kinase kinase 14 MAP3K14 NIK Serine/threonine-protein kinase NIK NF-kappa-beta-inducing kinase protein protein human Homo sapiens Human MAVMEMACPGAPGSAVGQQKELPKAKEKTPPLGKKQSSVYKLEAVEKSPVFCGKWEILNDVITKGTAKEGSEAGPAAISIIAQAECENSQEFSPTFSERIFIAGSKQYSQSESLDQIPNNVAHATEGKMARVCWKGKRRSKARKKRKKKSSKSLAHAGVALAKPLPRTPEQESCTIPVQEDESPLGAPYVRNTPQFTKPLKEPGLGQLCFKQLGEGLRPALPRSELHKLISPLQCLNHVWKLHHPQDGGPLPLPTHPFPYSRLPHPFPFHPLQPWKPHPLESFLGKLACVDSQKPLPDPHLSKLACVDSPKPLPGPHLEPSCLSRGAHEKFSVEEYLVHALQGSVSSGQAHSLTSLAKTWAARGSRSREPSPKTEDNEGVLLTEKLKPVDYEYREEVHWATHQLRLGRGSFGEVHRMEDKQTGFQCAVKKVRLEVFRAEELMACAGLTSPRIVPLYGAVREGPWVNIFMELLEGGSLGQLVKEQGCLPEDRALYYLGQALEGLEYLHSRRILHGDVKADNVLLSSDGSHAALCDFGHAVCLQPDGLGKSLLTGDYIPGTETHMAPEVVLGRSCDAKVDVWSSCCMMLHMLNGCHPWTQFFRGPLCLKIASEPPPVREIPPSCAPLTAQAIQEGLRKEPIHRVSAAELGGKVNRALQQVGGLKSPWRGEYKEPRHPPPNQANYHQTLHAQPRELSPRAPGPRPAEETTGRAPKLQPPLPPEPPEPNKSPPLTLSKEESGMWEPLPLSSLEPAPARNPSSPERKATVPEQELQQLEIELFLNSLSQPFSLEEQEQILSCLSIDSLSLSDDSEKNPSKASQSSRDTLSSGVHSWSSQAEARSSSWNMVLARGRPTDTPSYFNGVKVQIQSLNGEHLHIREFHRVKVGDIATGISSQIPAAAFSLVTKDGQPVRYDMEVPDSGIDLQCTLAPDGSFAWSWRVKHGQLENRP DIP protein Q99558 original sequence version: 159 C9D10F67FF7F48AC birc3_human Baculoviral IAP repeat-containing protein 3 Inhibitor of apoptosis protein 1 TNFR2-TRAF-signaling complex protein 1 IAP homolog C Apoptosis inhibitor 2 RING finger protein 49 BIRC3 API2 MIHC RNF49 RING-type E3 ubiquitin transferase BIRC3 Cellular inhibitor of apoptosis 2 protein protein human Homo sapiens Human MNIVENSIFLSNLMKSANTFELKYDLSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKRGDSPTEKHKKLYPSCRFVQSLNSVNNLEATSQPTFPSSVTNSTHSLLPGTENSGYFRGSYSNSPSNPVNSRANQDFSALMRSSYHCAMNNENARLLTFQTWPLTFLSPTDLAKAGFYYIGPGDRVACFACGGKLSNWEPKDNAMSEHLRHFPKCPFIENQLQDTSRYTVSNLSMQTHAARFKTFFNWPSSVLVNPEQLASAGFYYVGNSDDVKCFCCDGGLRCWESGDDPWVQHAKWFPRCEYLIRIKGQEFIRQVQASYPHLLEQLLSTSDSPGDENAESSIIHFEPGEDHSEDAIMMNTPVINAAVEMGFSRSLVKQTVQRKILATGENYRLVNDLVLDLLNAEDEIREEERERATEEKESNDLLLIRKNRMALFQHLTCVIPILDSLLTAGIINEQEHDVIKQKTQTSLQARELIDTILVKGNIAATVFRNSLQEAEAVLYEHLFVQQDIKYIPTEDVSDLPVEEQLRRLQEERTCKVCMDKEVSIVFIPCGHLVVCKDCAPSLRKCPICRSTIKGTVRTFLS DIP protein Q13489 original sequence version: 127 8581A00BA9AAB4A7 birc2_human Baculoviral IAP repeat-containing protein 2 Inhibitor of apoptosis protein 2 TNFR2-TRAF-signaling complex protein 2 IAP homolog B RING finger protein 48 BIRC2 API1 MIHB RNF48 RING-type E3 ubiquitin transferase BIRC2 Cellular inhibitor of apoptosis 1 protein protein human Homo sapiens Human MHKTASQRLFPGPSYQNIKSIMEDSTILSDWTNSNKQKMKYDFSCELYRMSTYSTFPAGVPVSERSLARAGFYYTGVNDKVKCFCCGLMLDNWKLGDSPIQKHKQLYPSCSFIQNLVSASLGSTSKNTSPMRNSFAHSLSPTLEHSSLFSGSYSSLSPNPLNSRAVEDISSSRTNPYSYAMSTEEARFLTYHMWPLTFLSPSELARAGFYYIGPGDRVACFACGGKLSNWEPKDDAMSEHRRHFPNCPFLENSLETLRFSISNLSMQTHAARMRTFMYWPSSVPVQPEQLASAGFYYVGRNDDVKCFCCDGGLRCWESGDDPWVEHAKWFPRCEFLIRMKGQEFVDEIQGRYPHLLEQLLSTSDTTGEENADPPIIHFGPGESSSEDAVMMNTPVVKSALEMGFNRDLVKQTVQSKILTTGENYKTVNDIVSALLNAEDEKREEEKEKQAEEMASDDLSLIRKNRMALFQQLTCVLPILDNLLKANVINKQEHDIIKQKTQIPLQARELIDTILVKGNAAANIFKNCLKEIDSTLYKNLFVDKNMKYIPTEDVSGLSLEEQLRRLQEERTCKVCMDKEVSVVFIPCGHLVVCQECAPSLRKCPICRGIIKGTVRTFLS C1778D328063586D mv1-birc2-1 2 5 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey bir2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Table 1, Supp. Table 1 MV1 is biotinylated to enable attachment mv1-birc2-2 2 11 unspecified role unspecified role prey prey bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role bait bait direct interaction direct interaction Table 1, Supp. Table 1 MV1 is biotinylated to enable attachment mv1-birc2-3 2 11 unspecified role unspecified role prey prey bir2_bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role bait bait direct interaction direct interaction Table 1, Supp. Table 1 MV1 is biotinylated to enable attachment bv6-birc2-1 2 7 unspecified role unspecified role bait bait 11 unspecified role unspecified role prey prey bir2_bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Table 1, Supp. Table 1 The bivalent inhibitor binds at the second BIR site KD=2.9E-6M BV6 is biotinylated to enable attachment mv1-birc4-1 2 8 unspecified role unspecified role prey prey bir2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role bait bait direct interaction direct interaction Table 1, Supp. Table 1 MV1 is biotinylated to enable attachment mv1-birc4-2 2 5 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Table 1, Supp. Table 1 MV1 is biotinylated to enable attachment mv1-birc4-3 2 8 unspecified role unspecified role prey prey bir2_bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role bait bait direct interaction direct interaction Table 1, Supp. Table 1 MV1 is biotinylated to enable attachment bv6-birc4-1 2 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey bir2_bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Table 1, Supp. Table 1 BV6 is biotinylated to enable attachment The bivalent inhibitor BV6 binds to the second Bir region KD=4.3E-6M mv1-birc2-4 3 5 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component bir2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Supp. Table 2 Ki=1.45E-5M mv1-birc2-5 3 5 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Supp. Table 2 Ki=4.0E-8M mv1-birc4-4 3 5 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component bir2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Supp. Table 2 Ki=4.83E-7M mv1-birc4-5 3 8 unspecified role unspecified role neutral component neutral component bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Supp. Table 2 Ki=3.73E-7M mv1-birc3-1 3 10 unspecified role unspecified role neutral component neutral component bir2 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Supp. Table 2 Ki=8.41E-6M mv1-birc3-2 3 10 unspecified role unspecified role neutral component neutral component bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Supp. Table 2 Ki=8.7E-8M bv6-birc2-2 3 7 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component bir2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Supp. Table 2 Ki=1.05E-5M bv6-birc2-3 3 7 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Supp. Table 2 Ki=1.69-7M bv6-birc4-6 3 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component bir2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Supp. Table 2 Ki=9.05E-7M bv6-birc4-2 3 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Supp. Table 2 Ki=4.86E-7M bv6-birc3-1 3 7 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component bir2 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Supp. Table 2 Ki=2.69E-6M bv6-birc3-2 3 7 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Supp. Table 2 Ki=2.34E-7M mv1-birc2-6 1 5 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component bir2_bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Table 1, Supp Table 3 bv6-birc2-4 1 11 unspecified role unspecified role neutral component neutral component bir2_bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 7 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Table 1, Table S3 In contrast, BV6 induced dimerization of c-IAP1 BIR2-BIR3, mv1-birc4-6 1 5 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component bir2_bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Table 1, Supp Fig. 3 bv6-birc4-7 1 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component bir2_bir3 region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) direct interaction direct interaction Table 1, Supp Table 3 birc2-map3k14-1 4 9 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined bir1 region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association 293T cells were transiently cotransfected with Myc-tagged NIK plasmid and indicated Flag-tagged c-IAP1 constructs. Fig. 6B, Supp Fig. 7 Exact coordinates of BIRC2 required to bind MAP3$14 not given in text but is described as the region required for binding to Traf proteins which is Bir1. birc4-casp9-1 4 6 unspecified role unspecified role prey prey p35 region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined certain certain sequence position certain 8 unspecified role unspecified role bait bait bir3 region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association IAP antagonists block XIAP-BIR3 binding to processed caspase-9. IAP antagonists such as MV1 and BV6 Sup Fig. S2 P98170 transfection; P55211 transfection;