IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The mitochondrial TOM complex is required for tBid/Bax-induced cytochrome c release. The mitochondrial TOM complex is required for tBid/Bax-induced cytochrome c release. J. Biol. Chem. (0021-9258) 2007 Ott M., Norberg E., Walter KM., Schreiner P., Kemper C., Rapaport D., Zhivotovsky B., Orrenius S. Apoptosis - Interactions involving proteins with a function related to apoptosis add apoptosis dataset tag The N. crassa TOM core complex (Tom40, Tom22, Tom5, Tom6 and Tom7) was incubated with tBID and BAX for 15 min at 30 C, followed by immunoprecipitation. Sten.Orrenius@ki.se 27-Aug-2008: Contacted by JYOTI. jyoti in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ott M., Norberg E., Walter KM., Schreiner P., Kemper C., Rapaport D., Zhivotovsky B., Orrenius S. Apoptosis - Interactions involving proteins with a function related to apoptosis add apoptosis dataset tag The N. crassa TOM core complex (Tom40, Tom22, Tom5, Tom6 and Tom7) was incubated with tBID and BAX for 15 min at 30 C, followed by immunoprecipitation. Sten.Orrenius@ki.se Accepted 2008-AUG-24 by JYOTI. 27-Aug-2008: Contacted by JYOTI. J. Biol. Chem. (0021-9258) 2007 jyoti The mitochondrial TOM complex is required for tBid/Bax-induced cytochrome c release. The mitochondrial TOM complex is required for tBid/Bax-induced cytochrome c release. J. Biol. Chem. (0021-9258) 2007 Ott M., Norberg E., Walter KM., Schreiner P., Kemper C., Rapaport D., Zhivotovsky B., Orrenius S. Apoptosis - Interactions involving proteins with a function related to apoptosis add apoptosis dataset tag The N. crassa TOM core complex (Tom40, Tom22, Tom5, Tom6 and Tom7) was incubated with tBID and BAX for 15 min at 30 C, followed by immunoprecipitation. Sten.Orrenius@ki.se 27-Aug-2008: Contacted by JYOTI. jyoti in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Ott M., Norberg E., Walter KM., Schreiner P., Kemper C., Rapaport D., Zhivotovsky B., Orrenius S. Apoptosis - Interactions involving proteins with a function related to apoptosis add apoptosis dataset tag The N. crassa TOM core complex (Tom40, Tom22, Tom5, Tom6 and Tom7) was incubated with tBID and BAX for 15 min at 30 C, followed by immunoprecipitation. Sten.Orrenius@ki.se Accepted 2008-AUG-24 by JYOTI. J. Biol. Chem. (0021-9258) 2007 jyoti tom40_neucr Mitochondrial import receptor subunit tom40 tom40 Protein MOM38 Translocase of outer membrane 40 kDa subunit mom38 B10H4.150 NCU01179 protein protein neucr Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) MASFSTESPLAMLRDNAIYSSLSDAFNAFQERRKQFGLSNPGTIETIAREVQRDTLLTNYMFSGLRADVTKAFSLAPLFQVSHQFAMGERLNPYAFAALYGTNQIFAQGNLDNEGALSTRFNYRWGDRTITKTQFSIGGGQDMAQFEHEHLGDDFSASLKAINPSFLDGGLTGIFVGDYLQAVTPRLGLGLQAVWQRQGLTQGPDTAISYFARYKAGDWVASAQLQAQGALNTSFWKKLTDRVQAGVDMTLSVAPSQSMMGGLTKEGITTFGAKYDFRMSTFRAQIDSKGKLSCLLEKRLGAAPVTLTFAADVDHVTQQAKLGMSVSIEASDVDLQEQQEGAQSLNIPF C17F11B576B36427 tom22_yeast Mitochondrial import receptor subunit TOM22 MOM22 TOM22 MAS17 MAS22 YNL131W N1217 N1862 Mitochondrial 22 kDa outer membrane protein Translocase of outer membrane 22 kDa subunit Protein MAS17 Mitochondrial 17 kDa assembly protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MVELTEIKDDVVQLDEPQFSRNQAIVEEKASATNNDVVDDEDDSDSDFEDEFDENETLLDRIVALKDIVPPGKRQTISNFFGFTSSFVRNAFTKSGNLAWTLTTTALLLGVPLSLSILAEQQLIEMEKTFDLQSDANNILAQGEKDAAATAN 6ACEC5D37D143CD3 6ACEC5D37D143CD3 tom40_yeast Mitochondrial import receptor subunit TOM40 TOM40 ISP42 YMR203W YM8325.04 MOM38 Mitochondrial import site protein ISP42 Translocase of outer membrane 40 kDa subunit protein protein yeast Saccharomyces cerevisiae Baker's yeast MSAPTPLAEASQIPTIPALSPLTAKQSKGNFFSSNPISSFVVDTYKQLHSHRQSLELVNPGTVENLNKEVSRDVFLSQYFFTGLRADLNKAFSMNPAFQTSHTFSIGSQALPKYAFSALFANDNLFAQGNIDNDLSVSGRLNYGWDKKNISKVNLQISDGQPTMCQLEQDYQASDFSVNVKTLNPSFSEKGEFTGVAVASFLQSVTPQLALGLETLYSRTDGSAPGDAGVSYLTRYVSKKQDWIFSGQLQANGALIASLWRKVAQNVEAGIETTLQAGMVPITDPLMGTPIGIQPTVEGSTTIGAKYEYRQSVYRGTLDSNGKVACFLERKVLPTLSVLFCGEIDHFKNDTKIGCGLQFETAGNQELLMLQQGLDADGNPLQALPQL 6436FBBA4020464B bax_human Apoptosis regulator BAX BAX BCL2L4 Bcl-2-like protein 4 protein protein human Homo sapiens Human MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG DIP protein Q07812 original sequence version: 73 DIP protein Q07812 original sequence version: 156 DIP protein Q07812 original sequence version: 92 DIP protein Q07812 original sequence version: 94 DIP protein Q07812-1 original sequence version: 93 DIP protein Q07812 original sequence version: 95 DIP protein Q07812 original sequence version: 120 DIP protein Q07812 original sequence version: 126 DIP protein Q07812 original sequence version: 143 DIP protein Q07812 original sequence version: 172 DIP protein Q07812 original sequence version: 175 6C0CDB0A7DEE4994 tom40-bax 2 3 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 3 A Antibodies against Tom40 recovered Tom40 and, to a lesser extent, Bax bax-tom40 2 6 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association 3 A antibodies against Bax could precipitate Bax together with a lower, but significant, amount of Tom40 tom22-bax 1 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey association association 3 B Antibodies against Tom22 allowed isolating Tom22 together with Tom40. Bax was only co-immunoprecipitated with the TOM complex from lysates of mitochondria that were exposed to Bax for 2 min but not from lysates of mitochondria that were incubated with Bax for 30 min BAX was coimmunoprecipitated in lysates from mitochondria exposed to BAX for 2 min but not after 30 min. The authors suggest this is because BAX interacts transiently with the TOM complex during its insertion into the outer mitochondrial membrane. tom22-tom40 1 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 3 B Antibodies against Tom22 allowed isolating Tom22 together with Tom40. BAX was coimmunoprecipitated in lysates from mitochondria exposed to BAX for 2 min but not after 30 min. The authors suggest this is because BAX interacts transiently with the TOM complex during its insertion into the outer mitochondrial membrane.