IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Proteasomes and RARS modulate AIMP1/EMAP II secretion in human cancer cell lines. Proteasomes and RARS modulate AIMP1/EMAP II secretion in human cancer cell lines. J. Cell. Physiol. (0021-9541) 2007 imex curation Bottoni A., Vignali C., Piccin D., Tagliati F., Luchin A., Zatelli MC., Uberti EC. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of RARS arginyl t-RNA synthetase taken from PMID:15648093 ti8@unife.it 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bottoni A., Vignali C., Piccin D., Tagliati F., Luchin A., Zatelli MC., Uberti EC. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of RARS arginyl t-RNA synthetase taken from PMID:15648093 ti8@unife.it Accepted 2010-JUL-7 by ORCHARD. J. Cell. Physiol. (0021-9541) 2007 orchard Only protein-protein interactions imex curation Proteasomes and RARS modulate AIMP1/EMAP II secretion in human cancer cell lines. Proteasomes and RARS modulate AIMP1/EMAP II secretion in human cancer cell lines. J. Cell. Physiol. (0021-9541) 2007 imex curation Bottoni A., Vignali C., Piccin D., Tagliati F., Luchin A., Zatelli MC., Uberti EC. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of RARS arginyl t-RNA synthetase taken from PMID:15648093 ti8@unife.it 25-Jun-2010: Contacted by JYOTI. orchard Only protein-protein interactions human-mcf7 Homo sapiens mammary carcinoma cells mcf7 mammary carcinoma cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bottoni A., Vignali C., Piccin D., Tagliati F., Luchin A., Zatelli MC., Uberti EC. Apoptosis - Interactions involving proteins with a function related to apoptosis Source of RARS arginyl t-RNA synthetase taken from PMID:15648093 ti8@unife.it Accepted 2010-JUL-7 by ORCHARD. 25-Jun-2010: Contacted by JYOTI. J. Cell. Physiol. (0021-9541) 2007 orchard Only protein-protein interactions imex curation syrc_human Arginine--tRNA ligase, cytoplasmic RARS1 Arginyl-tRNA synthetase RARS protein protein human Homo sapiens Human MDVLVSECSARLLQQEEEIKSLTAEIDRLKNCGCLGASPNLEQLQEENLKLKYRLNILRKSLQAERNKPTKNMINIISRLQEVFGHAIKAAYPDLENPPLLVTPSQQAKFGDYQCNSAMGISQMLKTKEQKVNPREIAENITKHLPDNECIEKVEIAGPGFINVHLRKDFVSEQLTSLLVNGVQLPALGENKKVIVDFSSPNIAKEMHVGHLRSTIIGESISRLFEFAGYDVLRLNHVGDWGTQFGMLIAHLQDKFPDYLTVSPPIGDLQVFYKESKKRFDTEEEFKKRAYQCVVLLQGKNPDITKAWKLICDVSRQELNKIYDALDVSLIERGESFYQDRMNDIVKEFEDRGFVQVDDGRKIVFVPGCSIPLTIVKSDGGYTYDTSDLAAIKQRLFEEKADMIIYVVDNGQSVHFQTIFAAAQMIGWYDPKVTRVFHAGFGVVLGEDKKKFKTRSGETVRLMDLLGEGLKRSMDKLKEKERDKVLTAEELNAAQTSVAYGCIKYADLSHNRLNDYIFSFDKMLDDRGNTAAYLLYAFTRIRSIARLANIDEEMLQKAARETKILLDHEKEWKLGRCILRFPEILQKILDDLFLHTLCDYIYELATAFTEFYDSCYCVEKDRQTGKILKVNMWRMLLCEAVAAVMAKGFDILGIKPVQRM FE9FB5C910709956 aimp1_human Aminoacyl tRNA synthase complex-interacting multifunctional protein 1 AIMP1 EMAP2 SCYE1 Multisynthase complex auxiliary component p43 protein protein human Homo sapiens Human MANNDAVLKRLEQKGAEADQIIEYLKQQVSLLKEKAILQATLREEKKLRVENAKLKKEIEELKQELIQAEIQNGVKQIPFPSGTPLHANSMVSENVIQSTAVTTVSSGTKEQIKGGTGDEKKAKEKIEKKGEKKEKKQQSIAGSADSKPIDVSRLDLRIGCIITARKHPDADSLYVEEVDVGEIAPRTVVSGLVNHVPLEQMQNRMVILLCNLKPAKMRGVLSQAMVMCASSPEKIEILAPPNGSVPGDRITFDAFPGEPDKELNPKKKIWEQIQPDLHTNDECVATYKGVPFEVKGKGVCRAQTMSNSGIK 5F0BF73E58810C60 syk_human Lysine--tRNA ligase KARS1 KARS KIAA0070 Lysyl-tRNA synthetase protein protein human Homo sapiens Human MAAVQAAEVKVDGSEPKLSKNELKRRLKAEKKVAEKEAKQKELSEKQLSQATAAATNHTTDNGVGPEEESVDPNQYYKIRSQAIHQLKVNGEDPYPHKFHVDISLTDFIQKYSHLQPGDHLTDITLKVAGRIHAKRASGGKLIFYDLRGEGVKLQVMANSRNYKSEEEFIHINNKLRRGDIIGVQGNPGKTKKGELSIIPYEITLLSPCLHMLPHLHFGLKDKETRYRQRYLDLILNDFVRQKFIIRSKIITYIRSFLDELGFLEIETPMMNIIPGGAVAKPFITYHNELDMNLYMRIAPELYHKMLVVGGIDRVYEIGRQFRNEGIDLTHNPEFTTCEFYMAYADYHDLMEITEKMVSGMVKHITGSYKVTYHPDGPEGQAYDVDFTPPFRRINMVEELEKALGMKLPETNLFETEETRKILDDICVAKAVECPPPRTTARLLDKLVGEFLEVTCINPTFICDHPQIMSPLAKWHRSKEGLTERFELFVMKKEICNAYTELNDPMRQRQLFEEQAKAKAAGDDEAMFIDENFCTALEYGLPPTAGWGMGIDRVAMFLTDSNNIKEVLLFPAMKPEDKKENVATTDTLESTTVGTSV DIP protein Q15046 original sequence version: 69 DIP protein Q15046 original sequence version: 116 DIP protein Q15046-1 original sequence version: 124 DIP protein Q15046-1 original sequence version: 129 DIP protein Q15046 original sequence version: 129 E7770953332D905D rars-aimp1 1 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait association association Fig 2. The amount of RARS associated with AIMP1 is significantly higher in HeLa and MCF7 cells over-expressing RARS as compared to the amount of RARS associated with AIMP1 in control cells. To demonstrate that the immunoprecipitated AIMP1 was part of the ARS complex, we performed a Western blot for KARS. rars-aimp1-1 2 4 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey 3 unspecified role unspecified role prey prey association association Fig 2. The amount of RARS associated with AIMP1 is significantly higher in HeLa and MCF7 cells over-expressing RARS as compared to the amount of RARS associated with AIMP1 in control cells. To demonstrate that the immunoprecipitated AIMP1 was part of the ARS complex, we performed a Western blot for KARS.