IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Proteome-wide Identification of HtrA2/Omi Substrates. Proteome-wide Identification of HtrA2/Omi Substrates. J. Proteome Res. (1535-3893) 2007 imex curation Vande Walle L., Van Damme P., Lamkanfi M., Saelens X., Vandekerckhove J., Gevaert K., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis peter.vandenabeele@dmbr.UGent.be 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protease assay protease assay weight by comassie molecular weight estimation by coomasie staining weight by comassie Vande Walle L., Van Damme P., Lamkanfi M., Saelens X., Vandekerckhove J., Gevaert K., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis peter.vandenabeele@dmbr.UGent.be Accepted 2012-JAN-16 by JYOTI J. Proteome Res. (1535-3893) 2007 Only protein-protein interactions imex curation Proteome-wide Identification of HtrA2/Omi Substrates. Proteome-wide Identification of HtrA2/Omi Substrates. J. Proteome Res. (1535-3893) 2007 imex curation Vande Walle L., Van Damme P., Lamkanfi M., Saelens X., Vandekerckhove J., Gevaert K., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis peter.vandenabeele@dmbr.UGent.be 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protease assay protease assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Vande Walle L., Van Damme P., Lamkanfi M., Saelens X., Vandekerckhove J., Gevaert K., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis This was performed as a COFRADIC experiment in which Jurkat cells extracts were submitted to protease cleavage by HtrA2 and an inactive HtrA2 and then differentially labelled to distinguish specifically cleaved peptides. peter.vandenabeele@dmbr.UGent.be Accepted 2012-JAN-16 by JYOTI 14-Mar-2012: Contacted by JYOTI. J. Proteome Res. (1535-3893) 2007 Only protein-protein interactions imex curation Proteome-wide Identification of HtrA2/Omi Substrates. Proteome-wide Identification of HtrA2/Omi Substrates. J. Proteome Res. (1535-3893) 2007 imex curation Vande Walle L., Van Damme P., Lamkanfi M., Saelens X., Vandekerckhove J., Gevaert K., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis peter.vandenabeele@dmbr.UGent.be 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro protease assay protease assay western blot western blot Immuno blot Immuno blot Vande Walle L., Van Damme P., Lamkanfi M., Saelens X., Vandekerckhove J., Gevaert K., Vandenabeele P. Apoptosis - Interactions involving proteins with a function related to apoptosis This was performed as a COFRADIC experiment in which Jurkat cells extracts were submitted to protease cleavage by HtrA2 and an inactive HtrA2 and then differentially labelled to distinguish specifically cleaved peptides. peter.vandenabeele@dmbr.UGent.be Accepted 2012-JAN-16 by JYOTI 14-Mar-2012: Contacted by JYOTI. J. Proteome Res. (1535-3893) 2007 Only protein-protein interactions imex curation tif1b_human Transcription intermediary factor 1-beta TRIM28 KAP1 RNF96 TIF1B Tripartite motif-containing protein 28 Nuclear corepressor KAP-1 KRAB-associated protein 1 KRAB-interacting protein 1 RING finger protein 96 E3 SUMO-protein ligase TRIM28 RING-type E3 ubiquitin transferase TIF1-beta protein protein human Homo sapiens Human MAASAAAASAAAASAASGSPGPGEGSAGGEKRSTAPSAAASASASAAASSPAGGGAEALELLEHCGVCRERLRPEREPRLLPCLHSACSACLGPAAPAAANSSGDGGAAGDGTVVDCPVCKQQCFSKDIVENYFMRDSGSKAATDAQDANQCCTSCEDNAPATSYCVECSEPLCETCVEAHQRVKYTKDHTVRSTGPAKSRDGERTVYCNVHKHEPLVLFCESCDTLTCRDCQLNAHKDHQYQFLEDAVRNQRKLLASLVKRLGDKHATLQKSTKEVRSSIRQVSDVQKRVQVDVKMAILQIMKELNKRGRVLVNDAQKVTEGQQERLERQHWTMTKIQKHQEHILRFASWALESDNNTALLLSKKLIYFQLHRALKMIVDPVEPHGEMKFQWDLNAWTKSAEAFGKIVAERPGTNSTGPAPMAPPRAPGPLSKQGSGSSQPMEVQEGYGFGSGDDPYSSAEPHVSGVKRSRSGEGEVSGLMRKVPRVSLERLDLDLTADSQPPVFKVFPGSTTEDYNLIVIERGAAAAATGQPGTAPAGTPGAPPLAGMAIVKEEETEAAIGAPPTATEGPETKPVLMALAEGPGAEGPRLASPSGSTSSGLEVVAPEGTSAPGGGPGTLDDSATICRVCQKPGDLVMCNQCEFCFHLDCHLPALQDVPGEEWSCSLCHVLPDLKEEDGSLSLDGADSTGVVAKLSPANQRKCERVLLALFCHEPCRPLHQLATDSTFSLDQPGGTLDLTLIRARLQEKLSPPYSSPQEFAQDVGRMFKQFNKLTEDKADVQSIIGLQRFFETRMNEAFGDTKFSAVLVEPPPMSLPGAGLSSQELSGGPGDGP DIP protein Q13263 original sequence version: 156 DIP protein Q13263 original sequence version: 154 DIP protein Q13263-1 original sequence version: 143 DIP protein Q13263 original sequence version: 198 DIP protein Q13263 original sequence version: 155 2027BABB7C94FE20 o43464-pro_0000026946 Serine protease HTRA2, mitochondrial OMI PRSS25 HTRA2 Omi stress-regulated endoprotease High temperature requirement protein A2 Serine protease 25 Serine proteinase OMI protein protein human Homo sapiens Human AVPSPPPASPRSQYNFIADVVEKTAPAVVYIEILDRHPFLGREVPISNGSGFVVAADGLIVTNAHVVADRRRVRVRLLSGDTYEAVVTAVDPVADIATLRIQTKEPLPTLPLGRSADVRQGEFVVAMGSPFALQNTITSGIVSSAQRPARDLGLPQTNVEYIQTDAAIDFGNSGGPLVNLDGEVIGVNTMKVTAGISFAIPSDRLREFLHRGEKKNSSSGISGSQRRYIGVMMLTLSPSILAELQLREPSFPDVQHGVLIHKVILGSPAHRAGLRPGDVILAIGEQMVQNAEDVYEAVRTQSQLAVQIRRGRETLTLYVTPEVTE 134 458 0F60587BB70D22CB if4g1_human Eukaryotic translation initiation factor 4 gamma 1 p220 EIF4G1 EIF4G EIF4GI EIF4F protein protein human Homo sapiens Human MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPTPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN DIP protein Q04637 original sequence version: 68 DIP protein Q04637 original sequence version: 166 DIP protein Q04637 original sequence version: 160 DIP protein Q04637-1 original sequence version: 94 DIP protein Q04637 original sequence version: 115 DIP protein Q04637 original sequence version: 119 DIP protein Q04637 original sequence version: 146 DIP protein Q04637-1 original sequence version: 148 DIP protein Q04637 original sequence version: 150 324088B60863DA34 vime_human Vimentin VIM protein protein human Homo sapiens Human MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE DIP protein P08670 original sequence version: 195 DIP protein P08670 original sequence version: 209 DIP protein P08670 original sequence version: 192 DIP protein P08670 original sequence version: 175 BAB54026665B015A q86xe2_human PDXDC1 protein protein human Homo sapiens Human MDASLEKIADPTLAEMGKNLKEAVKMLEDSQRRTEEENGKKLISGDIPGPLQGSGQDMVSILQLVQNLMHGDEDEEPQSPRIQNIGEQGHMALLGHSLGAYISTLDKEKLRKLTTRILSDTTLWLCRIFRYENGCAYFHEEEREGLAKICRLAIHSRYEDFVVDGFNVLYNKKPVIYLSAAARPGLGQYLCNQLGLPFPCLCRVPCNTVFGSQHQMDVAFLEKLIKDDIERGRLPLLLVANAGTAAVGHTDKIGRLKELCEQYGIWLHVEGVNLATLALGYVSSSVLAAAKCDSMTMTPGPWLGLPAVPAVTLYKHDDPALTLVAGLTSNKPTDKLRALPLWLSLQYLGLDGFVERIKHACQLSQRLQESLKKVNYIKILVEDELSSPVVVFRFFQELPGSDPVFKAVPVPNMTPSGVGRERHSCDALNRWLGEQLKQLVPASGLTVMDLEAEGTCLRFSPLMTAAGWSHCGTTTRYDCVRSRLTTRLPGSTGEQAPP 84A47B0228909E0A casb_bovin Beta-casein CSN2 protein protein bovin Bos taurus (Bovine) Bovine MKVLILACLVALALARELEELNVPGEIVESLSSSEESITRINKKIEKFQSEEQQQTEDELQDKIHPFAQTQSLVYPFPGPIPNSLPQNIPPLTQTPVVVPPFLQPEVMGVSKVKEAMAPKHKEMPFPKYPVEPFTESQSLTLTDVENLHLPLPLLQSWMHQPHQPLPPTVMFPPQSVLSLSQSKVLPVPQKAVPYPQRDMPIQAFLLYQEPVLGPVRGPFPIIV DIP protein P02666 original sequence version: 88 DIP protein P02666 original sequence version: 101 F0BBDD8148A238AE hcd2_human 3-hydroxyacyl-CoA dehydrogenase type-2 HSD17B10 SCHAD XH98G2 HADH2 3-hydroxyacyl-CoA dehydrogenase type II Type II HADH 3-hydroxy-2-methylbutyryl-CoA dehydrogenase Endoplasmic reticulum-associated amyloid beta-peptide-binding protein Short-chain type dehydrogenase/reductase XH98G2 Mitochondrial ribonuclease P protein 2 2-methyl-3-hydroxybutyryl-CoA dehydrogenase ERAB MRPP2 SDR5C1 Short chain dehydrogenase/reductase family 5C member 1 17-beta-estradiol 17-dehydrogenase 3-alpha-(17-beta)-hydroxysteroid dehydrogenase (NAD(+)) 3alpha(or 20beta)-hydroxysteroid dehydrogenase 7-alpha-hydroxysteroid dehydrogenase protein protein human Homo sapiens Human MAAACRSVKGLVAVITGGASGLGLATAERLVGQGASAVLLDLPNSGGEAQAKKLGNNCVFAPADVTSEKDVQTALALAKGKFGRVDVAVNCAGIAVASKTYNLKKGQTHTLEDFQRVLDVNLMGTFNVIRLVAGEMGQNEPDQGGQRGVIINTASVAAFEGQVGQAAYSASKGGIVGMTLPIARDLAPIGIRVMTIAPGLFGTPLLTSLPEKVCNFLASQVPFPSRLGDPAEYAHLVQAIIENPFLNGEVIRLDGAIRMQP 9E74F242E3E6FEF1 ef1a1_human Elongation factor 1-alpha 1 EEF1A1 EEF1A EF1A LENG7 Eukaryotic elongation factor 1 A-1 Elongation factor Tu Leukocyte receptor cluster member 7 protein protein human Homo sapiens Human MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK DIP protein P68104 original sequence version: null DIP protein P68104 original sequence version: 134 D465615545AF686A ccar2_human Cell cycle and apoptosis regulator protein 2 CCAR2 Cell division cycle and apoptosis regulator protein 2 DBC1 DBIRD complex subunit KIAA1967 Deleted in breast cancer gene 1 protein KIAA1967 p30 DBC NET35 protein protein human Homo sapiens Human MSQFKRQRINPLPGGRNFSGTASTSLLGPPPGLLTPPVATELSQNARHLQGGEKQRVFTGIVTSLHDYFGVVDEEVFFQLSVVKGRLPQLGEKVLVKAAYNPGQAVPWNAVKVQTLSNQPLLKSPAPPLLHVAALGQKQGILGAQPQLIFQPHRIPPLFPQKPLSLFQTSHTLHLSHLNRFPARGPHGRLDQGRSDDYDSKKRKQRAGGEPWGAKKPRHDLPPYRVHLTPYTVDSPICDFLELQRRYRSLLVPSDFLSVHLSWLSAFPLSQPFSLHHPSRIQVSSEKEAAPDAGAEPITADSDPAYSSKVLLLSSPGLEELYRCCMLFVDDMAEPRETPEHPLKQIKFLLGRKEEEAVLVGGEWSPSLDGLDPQADPQVLVRTAIRCAQAQTGIDLSGCTKWWRFAEFQYLQPGPPRRLQTVVVYLPDVWTIMPTLEEWEALCQQKAAEAAPPTQEAQGETEPTEQAPDALEQAADTSRRNAETPEATTQQETDTDLPEAPPPPLEPAVIARPGCVNLSLHGIVEDRRPKERISFEVMVLAELFLEMLQRDFGYRVYKMLLSLPEKVVSPPEPEKEEAAKEEATKEEEAIKEEVVKEPKDEAQNEGPATESEAPLKEDGLLPKPLSSGGEEEEKPRGEASEDLCEMALDPELLLLRDDGEEEFAGAKLEDSEVRSVASNQSEMEFSSLQDMPKELDPSAVLPLDCLLAFVFFDANWCGYLHRRDLERILLTLGIRLSAEQAKQLVSRVVTQNICQYRSLQYSRQEGLDGGLPEEVLFGNLDLLPPPGKSTKPGAAPTEHKALVSHNGSLINVGSLLQRAEQQDSGRLYLENKIHTLELKLEESHNRFSATEVTNKTLAAEMQELRVRLAEAEETARTAERQKSQLQRLLQELRRRLTPLQLEIQRVVEKADSWVEKEEPAPSN DIP protein Q8N163 original sequence version: 96 DIP protein Q8N163 original sequence version: 139 1733934377E35D21 tba1a_human Tubulin alpha-1A chain TUBA1A Tubulin B-alpha-1 Tubulin alpha-3 chain Alpha-tubulin 3 TUBA3 protein protein human Homo sapiens Human MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLIGQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY DIP protein Q71U36 original sequence version: 111 00F8429A4A10E5FE acts_human Actin, alpha skeletal muscle ACTA1 ACTA Alpha-actin-1 protein protein human Homo sapiens Human MCDEDETTALVCDNGSGLVKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPTLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRLDLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEKSYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNVMSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWITKQEYDEAGPSIVHRKCF DIP protein P68133 original sequence version: null DF2A3A046346A179 csn2-htra2-3 1 5 enzyme enzyme unspecified role unspecified role purified purified O43464-PRO_0000026946:p.Ser173Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:52:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser173ala' Tue Jun 30 19:16:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser173Ala' his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 9 enzyme target enzyme target substrate substrate unspecified role unspecified role purified purified bovin Bos taurus (Bovine) Bovine protein cleavage protein cleavage 1b and 1c Protease inhibitor Ucf-101 can inhibit the reaction. acta1-htra2 3 5 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O43464-PRO_0000026946:p.Ser173Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 16:04:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser173ala' Tue Jun 30 16:37:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser173Ala' ecoli Escherichia coli (strain K12) 14 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate human-jurkat Homo sapiens jurkat Human T cell leukemia protein cleavage protein cleavage 3a eef1a1-htra2-1 3 11 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate human-jurkat Homo sapiens jurkat Human T cell leukemia 5 enzyme enzyme unspecified role unspecified role purified purified O43464-PRO_0000026946:p.Ser173Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 18:07:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser173ala' Tue Jun 30 18:48:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser173Ala' his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 3a htra2-tuba1a 3 5 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O43464-PRO_0000026946:p.Ser173Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:39:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser173ala' Tue Jun 30 17:39:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser173Ala' ecoli Escherichia coli (strain K12) 13 enzyme target enzyme target substrate substrate unspecified role unspecified role protein cleavage protein cleavage 3a htra2-trim28-1 3 5 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O43464-PRO_0000026946:p.Ser173Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 18:55:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser173ala' Tue Jun 30 17:32:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser173Ala' ecoli Escherichia coli (strain K12) 4 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate human-jurkat Homo sapiens jurkat Human T cell leukemia protein cleavage protein cleavage 3a eif4g1-htra2-1 3 6 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate human-jurkat Homo sapiens jurkat Human T cell leukemia 5 enzyme enzyme unspecified role unspecified role purified purified O43464-PRO_0000026946:p.Ser173Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 16:19:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser173ala' Tue Jun 30 17:31:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser173Ala' his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 3a htra2-vim-1 2 5 enzyme enzyme unspecified role unspecified role purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range O43464-PRO_0000026946:p.Ser173Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:28:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser173ala' Tue Jun 30 18:00:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser173Ala' ecoli Escherichia coli (strain K12) 7 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage 3b htra2-hcd2 2 10 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 5 enzyme enzyme unspecified role unspecified role purified purified O43464-PRO_0000026946:p.Ser173Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 15:02:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser173ala' Tue Jun 30 18:09:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser173Ala' his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 3b hsd17b10-htra2-1 2 5 enzyme enzyme unspecified role unspecified role purified purified O43464-PRO_0000026946:p.Ser173Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:07:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser173ala' Tue Jun 30 18:20:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser173Ala' his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 12 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes protein cleavage protein cleavage 3b htra2-kiaa0251 2 8 enzyme target enzyme target substrate substrate unspecified role unspecified role cell lysate cell lysate [35s] labelling 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined rabit-reticulocyte Oryctolagus cuniculus reticulocyte reticulocyte Reticulocytes 5 enzyme enzyme unspecified role unspecified role purified purified O43464-PRO_0000026946:p.Ser173Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A Wed May 02 18:04:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser173ala' Tue Jun 30 15:54:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser173Ala' his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) protein cleavage protein cleavage 3b