IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Essential role for nuclear PTEN in maintaining chromosomal integrity. Essential role for nuclear PTEN in maintaining chromosomal integrity. Cell (0092-8674) 2007 imex curation Shen WH., Balajee AS., Wang J., Wu H., Eng C., Pandolfi PP., Yin Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Some of the coimmunoprecipitations could not be curated as general anti-centromere antibodies were used and not anti-CENP-C. yy151@columbia.edu 10-APR-2007: Contacted by JYOTI. Only protein-protein interactions 4 mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Shen WH., Balajee AS., Wang J., Wu H., Eng C., Pandolfi PP., Yin Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Some of the coimmunoprecipitations could not be curated as general anti-centromere antibodies were used and not anti-CENP-C. yy151@columbia.edu Accepted 2007-APR-6 by JYOTI. 10-APR-2007: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 4 imex curation Essential role for nuclear PTEN in maintaining chromosomal integrity. Essential role for nuclear PTEN in maintaining chromosomal integrity. Cell (0092-8674) 2007 imex curation Shen WH., Balajee AS., Wang J., Wu H., Eng C., Pandolfi PP., Yin Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Some of the coimmunoprecipitations could not be curated as general anti-centromere antibodies were used and not anti-CENP-C. yy151@columbia.edu 10-APR-2007: Contacted by JYOTI. Only protein-protein interactions 4 mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Shen WH., Balajee AS., Wang J., Wu H., Eng C., Pandolfi PP., Yin Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Some of the coimmunoprecipitations could not be curated as general anti-centromere antibodies were used and not anti-CENP-C. yy151@columbia.edu Accepted 2007-APR-6 by JYOTI. 10-APR-2007: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 4 imex curation Essential role for nuclear PTEN in maintaining chromosomal integrity. Essential role for nuclear PTEN in maintaining chromosomal integrity. Cell (0092-8674) 2007 imex curation Shen WH., Balajee AS., Wang J., Wu H., Eng C., Pandolfi PP., Yin Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Some of the coimmunoprecipitations could not be curated as general anti-centromere antibodies were used and not anti-CENP-C. yy151@columbia.edu 10-APR-2007: Contacted by JYOTI. Only protein-protein interactions 4 mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Shen WH., Balajee AS., Wang J., Wu H., Eng C., Pandolfi PP., Yin Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Some of the coimmunoprecipitations could not be curated as general anti-centromere antibodies were used and not anti-CENP-C. yy151@columbia.edu Accepted 2007-APR-6 by JYOTI. 10-APR-2007: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 4 imex curation Essential role for nuclear PTEN in maintaining chromosomal integrity. Essential role for nuclear PTEN in maintaining chromosomal integrity. Cell (0092-8674) 2007 imex curation Shen WH., Balajee AS., Wang J., Wu H., Eng C., Pandolfi PP., Yin Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Some of the coimmunoprecipitations could not be curated as general anti-centromere antibodies were used and not anti-CENP-C. yy151@columbia.edu 10-APR-2007: Contacted by JYOTI. Only protein-protein interactions 4 mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Shen WH., Balajee AS., Wang J., Wu H., Eng C., Pandolfi PP., Yin Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Some of the coimmunoprecipitations could not be curated as general anti-centromere antibodies were used and not anti-CENP-C. yy151@columbia.edu Accepted 2007-APR-6 by JYOTI. 10-APR-2007: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 4 imex curation Essential role for nuclear PTEN in maintaining chromosomal integrity. Essential role for nuclear PTEN in maintaining chromosomal integrity. Cell (0092-8674) 2007 imex curation Shen WH., Balajee AS., Wang J., Wu H., Eng C., Pandolfi PP., Yin Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer Some of the coimmunoprecipitations could not be curated as general anti-centromere antibodies were used and not anti-CENP-C. yy151@columbia.edu 10-APR-2007: Contacted by JYOTI. Only protein-protein interactions 4 human-pc3 Human PC3 Caucasian prostate adenocarcinoma pc3 Human Caucasian prostate adenocarcinoma ch-ip chromatin immunoprecipitation assay ch-ip primer specific pcr primer specific pcr Shen WH., Balajee AS., Wang J., Wu H., Eng C., Pandolfi PP., Yin Y. Apoptosis - Interactions involving proteins with a function related to apoptosis Some of the coimmunoprecipitations could not be curated as general anti-centromere antibodies were used and not anti-CENP-C. The two primers were used in BLAST searches to find the DNA sequence. yy151@columbia.edu Accepted 2007-APR-6 by JYOTI. 10-APR-2007: Contacted by JYOTI. Cell (0092-8674) 2007 Only protein-protein interactions 4 imex curation rad51_mouse_gene DNA repair protein RAD51 homolog 1 Rad51 Rad51a Reca RAD51 homolog A gene gene mouse Mus musculus Mouse pten_mouse Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Mutated in multiple advanced cancers 1 Pten Mmac1 Phosphatase and tensin homolog protein protein mouse Mus musculus Mouse MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHSQITKV DIP protein O08586 original sequence version: 125 DIP protein O08586 original sequence version: 81 DIP protein O08586 original sequence version: 129 DIP protein O08586 original sequence version: 154 75F97C3DD6843BA9 rad51_human_gene RAD51_HUMAN_GENE gene gene human Homo sapiens Human e2f1_mouse Transcription factor E2F1 E2f1 protein protein mouse Mus musculus Mouse MAVAPAGGQHAPALEALLGAGALRLLDSSQIVIISTAPDVGAPQLPAAPPTGPRDSDVLLFATPQAPRPAPSAPRPALGRPPVKRRLDLETDHQYLAGSSGPFRGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSRSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSHTMVGIGKRLEGLTQDLQQLQESEQQLDHLMHICTTQLQLLSEDSDTQRLAYVTCQDLRSIADPAEQMVIVIKAPPETQLQAVDSSETFQISLKSKQGPIDVFLCPEESADGISPGKTSCQETSSGEDRTADSGPAGPPPSPPSTSPALDPSQSLLGLEQEAVLPRMGHLRVPMEEDQLSPLVAADSLLEHVKEDFSGLLPGEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF C5DF18AD3B4DFEFA cenpc_mouse Centromere protein C Cenpc Cenpc1 Centromere autoantigen C Centromere protein C 1 protein protein mouse Mus musculus Mouse MASFHLDHLKNYHRRYCRSSRAPNIHTKKGQNMLEILQDCFEDQSKASFLDDFTESLTSSTQKKKANYSQSSSKKCPESHSKPVPVSSRTGEASLQASAEPSEAAGGSVQANEVHHGASDELDLCVGSPVVLLDANVNTLQKAASPAGQKRVASVSRSPVDRQASNKNISFKTRKRLNFEDKVTLSTAETENSVLQVEDNLSKGQEGTSSEITQKRDDLSSDVQSRSKKNFSELFLETVKRKSKSSSVVRHTAAVPFSPPPPSDMKLLEDEFIIDRSDRSFSSRLWVMIPSKDRHLSAHKPSPENTALLQGKKSREKSHSLSAMTFARNTQSDKAHPIEEAQLSVEENPATTCTDELENDCRSPENKMQSETAKTPPAWERTTKQSQRRVSKPKAAEELRKGQSSWENSNVSNTGQDKLQINSKRNMKDCEEVRNEPNPKKQKPALENKKKTNSTQTNKEKSGKKFFSGGSKNKFVPKKVTLTSRRSCRISQRPSEWWRVKSDESSVDRNPSKENNSPVVYPNKKKQTKRNHVSKRAGKKPGSSKRQKTEMSPRVQKSLNVKDSGGTVSGHDDTSRSQRKPLKIIEADPTQKSLAISRPKRGCKYRNNVMTSPNVHLKSHTEEYTSKTQMESASNSEMSKRSVWEESGPSRFKNYEMPGSSNSEMGDEQDQKSLHFTTRSFNMVPDKKLHHKLVLPSNSPNVRRSNRIRLKPLEYWRGERVDYQESSSGQLVLEIISPSSVPTKIKAQRNLGKVNKKVTKKPTHLNSHEKAKMELPLDMRLGDPFQATLAKDPETAELVPMDLIRPRDTYRFFVEQHGLKVFKTLDTIYFSTGKLVLGPYEEKGKQHVGQDILVFYVNFGDLLCTLHETPYKLTTGDSFYVPSGNHYNIKNLLNVESSLLFTQIKR 0DCFE76496B33946 pten_human Phosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN Phosphatase and tensin homolog Mutated in multiple advanced cancers 1 PTEN MMAC1 TEP1 protein protein human Homo sapiens Human MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV 75F97C3DD6802BA9 pten-cenpc1 1 7 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association 2 C, 4 G We wished to determine whether the PTEN-associated 140 kDa protein that was recognized by the anti-centromere antibody (Figure 2B) could in fact be CENP-C. This possibility was confirmed by using a CENP-C-specific antibody that detected the 140 kDa PTEN-associated protein in Pten+/+ MEFs The PTEN-CENP-C interaction is markedly disrupted in MEFs also expressing human PTEN (aa 1-188). cenpc1-pten 1 10 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 2 D PTEN was detected in an anti-CENP-C immunoprecipitated complex from Pten+/+ MEFs This interaction occurs in p53+/+ and p53-/- MEFs. cenpc1-pten-1 2 11 unspecified role unspecified role prey prey c-terminal required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role bait bait physical association physical association 3 B, C P60484 tranfection The results shown in Figures 3A and 3C indicate that the C-terminal domain of PTEN is required for its association with centromeres and CENP-C since the truncated mutant PTEN189 no longer interacts pten-pten 3 7 unspecified role unspecified role prey prey 11 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association ectopic PTEN189 interacts with endogenous wild-type PTEN 4 H P60484 transfection pten-rad51_human_dna 5 8 unspecified role unspecified role prey prey promoter_region binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait association association 6 F the PTEN-bound Rad51 promoter was detected from chromatin of PC-3 cells pten-rad51-1 4 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait association association 5 G the mouse Rad51 promoter was PCR-amplified from ChIP products of Pten+/+ MEFs The primers given did not match the mouse RAD51 gene in a BLAST search, although they did match the human RAD51 gene. e2f1-rad51-1 4 6 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait association association 7 C The primers given did not match the mouse RAD51 gene in a BLAST search, although they did match the human RAD51 gene. the Rad51 promoter was amplified from the anti- E2F-1 antibody immunoprecipitates from E2F-1+/+ MEFs