IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Drosophila IKK-related kinase regulates nonapoptotic function of caspases via degradation of IAPs. Drosophila IKK-related kinase regulates nonapoptotic function of caspases via degradation of IAPs. Cell (0092-8674) 2006 imex curation Kuranaga E., Kanuka H., Tonoki A., Takemoto K., Tomioka T., Kobayashi M., Hayashi S., Miura M. Apoptosis - Interactions involving proteins with a function related to apoptosis miura@mol.f.u-tokyo.ac.jp 25-SEP-2006: Contacted by JYOTI. Only protein-protein interactions 6 drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kuranaga E., Kanuka H., Tonoki A., Takemoto K., Tomioka T., Kobayashi M., Hayashi S., Miura M. Apoptosis - Interactions involving proteins with a function related to apoptosis miura@mol.f.u-tokyo.ac.jp Accepted 2006-SEP-7 by DAVET. 25-SEP-2006: Contacted by JYOTI. Cell (0092-8674) 2006 Only protein-protein interactions 6 imex curation Drosophila IKK-related kinase regulates nonapoptotic function of caspases via degradation of IAPs. Drosophila IKK-related kinase regulates nonapoptotic function of caspases via degradation of IAPs. Cell (0092-8674) 2006 imex curation Kuranaga E., Kanuka H., Tonoki A., Takemoto K., Tomioka T., Kobayashi M., Hayashi S., Miura M. Apoptosis - Interactions involving proteins with a function related to apoptosis miura@mol.f.u-tokyo.ac.jp 25-SEP-2006: Contacted by JYOTI. Only protein-protein interactions 6 drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kuranaga E., Kanuka H., Tonoki A., Takemoto K., Tomioka T., Kobayashi M., Hayashi S., Miura M. Apoptosis - Interactions involving proteins with a function related to apoptosis miura@mol.f.u-tokyo.ac.jp Accepted 2006-SEP-7 by DAVET. 25-SEP-2006: Contacted by JYOTI. Cell (0092-8674) 2006 Only protein-protein interactions 6 imex curation Drosophila IKK-related kinase regulates nonapoptotic function of caspases via degradation of IAPs. Drosophila IKK-related kinase regulates nonapoptotic function of caspases via degradation of IAPs. Cell (0092-8674) 2006 imex curation Kuranaga E., Kanuka H., Tonoki A., Takemoto K., Tomioka T., Kobayashi M., Hayashi S., Miura M. Apoptosis - Interactions involving proteins with a function related to apoptosis miura@mol.f.u-tokyo.ac.jp 25-SEP-2006: Contacted by JYOTI. Only protein-protein interactions 6 in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Kuranaga E., Kanuka H., Tonoki A., Takemoto K., Tomioka T., Kobayashi M., Hayashi S., Miura M. Apoptosis - Interactions involving proteins with a function related to apoptosis miura@mol.f.u-tokyo.ac.jp Accepted 2006-SEP-7 by DAVET. 25-SEP-2006: Contacted by JYOTI. Cell (0092-8674) 2006 Only protein-protein interactions 6 imex curation xiap_human E3 ubiquitin-protein ligase XIAP Inhibitor of apoptosis protein 3 X-linked inhibitor of apoptosis protein IAP-like protein XIAP API3 IAP3 BIRC4 Baculoviral IAP repeat-containing protein 4 RING-type E3 ubiquitin transferase XIAP protein protein human Homo sapiens Human MTFNSFEGSKTCVPADINKEEEFVEEFNRLKTFANFPSGSPVSASTLARAGFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLENSATQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDISDTIYPRNPAMYSEEARLKSFQNWPDYAHLTPRELASAGLYYTGIGDQVQCFCCGGKLKNWEPCDRAWSEHRRHFPNCFFVLGRNLNIRSESDAVSSDRNFPNSTNLPRNPSMADYEARIFTFGTWIYSVNKEQLARAGFYALGEGDKVKCFHCGGGLTDWKPSEDPWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEECLVRTTEKTPSLTRRIDDTIFQNPMVQEAIRMGFSFKDIKKIMEEKIQISGSNYKSLEVLVADLVNAQKDSMQDESSQTSLQKEISTEEQLRRLQEEKLCKICMDRNIAIVFVPCGHLVTCKQCAEAVDKCPMCYTVITFKQKIFMS DIP protein P98170 original sequence version: 168 DIP protein P98170 original sequence version: 118 DIP protein P98170 original sequence version: 153 9D394C16D45EB635 diap1_drome Death-associated inhibitor of apoptosis 1 Diap1 Apoptosis 1 inhibitor E3 ubiquitin-protein ligase th Iap1 Inhibitor of apoptosis 1 Protein thread th CG12284 RING-type E3 ubiquitin transferase Diap1 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MASVVADLPSYGPIAFDQVDNNTNATQLFKNNINKTRMNDLNREETRLKTFTDWPLDWLDKRQLAQTGMYFTHAGDKVKCFFCGVEIGCWEQEDQPVPEHQRWSPNCPLLRRRTTNNVPINAEALDRILPPISYDICGANDSTLEMREHAYAEGVIPMSQLIQSIGMNAVNAAGSVTGTAAPQPRVTVATHASTATQATGDVQPETCRPSAASGNYFPQYPEYAIETARLRTFEAWPRNLKQKPHQLAEAGFFYTGVGDRVRCFSCGGGLMDWNDNDEPWEQHALWLSQCRFVKLMKGQLYIDTVAAKPVLAEEKEESSSIGGVAVASTQASEEEQQTSLSSEEAVSGDVAPSVAPTAATRIFNKIVEATAVATPSTNSSGSTSIPEEKLCKICYGAEYNTAFLPCGHVVACAKCASSVTKCPLCRKPFTDVMRVYFS 24CA8BC13F5DEF31 tbk1_human Serine/threonine-protein kinase TBK1 TBK1 NAK TANK-binding kinase 1 T2K NF-kappa-B-activating kinase protein protein human Homo sapiens Human MQSTSNHLWLLSDILGQGATANVFRGRHKKTGDLFAIKVFNNISFLRPVDVQMREFEVLKKLNHKNIVKLFAIEEETTTRHKVLIMEFCPCGSLYTVLEEPSNAYGLPESEFLIVLRDVVGGMNHLRENGIVHRDIKPGNIMRVIGEDGQSVYKLTDFGAARELEDDEQFVSLYGTEEYLHPDMYERAVLRKDHQKKYGATVDLWSIGVTFYHAATGSLPFRPFEGPRRNKEVMYKIITGKPSGAISGVQKAENGPIDWSGDMPVSCSLSRGLQVLLTPVLANILEADQEKCWGFDQFFAETSDILHRMVIHVFSLQQMTAHKIYIHSYNTATIFHELVYKQTKIISSNQELIYEGRRLVLEPGRLAQHFPKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDASMAKAITGVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTEVVITLDFCIRNIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQGTIETSLQDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIYYQFKKDKAERRLAYNEEQIHKFDKQKLYYHATKAMTHFTDECVKKYEAFLNKSEEWIRKMLHLRKQLLSLTNQCFDIEEEVSKYQEYTNELQETLPQKMFTASSGIKHTMTPIYPSSNTLVEMTLGMKKLKEEMEGVVKELAENNHILERFGSLTMDGGLRNVDCL DIP protein Q9UHD2 original sequence version: 116 DIP protein Q9UHD2 original sequence version: 120 DIP protein Q9UHD2 original sequence version: 79 DIP protein Q9UHD2 original sequence version: null B58E4FE1B502276D q86pe7_drome IKKepsilon ik2 CG2615 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSFLRGSVSYVWCTTSVLGKGATGSVFQGVNKITGESVAVKTFNPYSHMRPADVQMREFEALKKVNHENIVKLLAIEEDQEGRGKVIVMELCTGGSLFNILDDPENSYGLPEHEFLLVLEHLCAGMKHLRDNKLVHRDLKPGNIMKFISEDGQTIYKLTDFGAARELEDNQPFASLYGTEEYLHPDLYERAVLRKSIQRSFTANVDLWSIGVTLYHVATGNLPFRPFGGRKNRETMHQITTKKASGVISGTQLSENGPIEWSTTLPPHAHLSQGLKTLVTPLLAGLLEENREKTWSFDRFFHEVTLILRKRVIHVFFTNRTSSVEVFLEPDEQIDNFRERIFLQTEVPLEKQILLFNNEHLEKKVTPRTIAKAFPATTTEQPIFLYSNDDNNVQLPQQLDLPKFPVFPPNVSVENDASLAKSACSVGHECKRRVDIFTSMDILIKKGVEHFIEMLVTTITLLLKKTESFDNLLSTVIDYADVVHSMARVTKGDQEIKTLLTALENVKSDFDGAADVISQMHKHFVIDDELNDQWTSSMHGKKCPCKTRASAQAKYLVERLRDSWQHLLRDRATRTLTYNDEQFHALEKIKVDHNGKRIKALLLDNVNPTVAQIAECLADWYKLAQTVYLKTQILEKDVRDCERKLNGIRDELYHVKSELKLDVDTKTINNNNQLAKIEERNRLRVMQQQQQEVMAVMRTNSDIISLLSKLGITNGSLESS 91058A2B4F0C7A58 ik2-th 2 7 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association When the S2 cells were treated with DmIKK epsilon dsRNA endogenous DIAP1 was not immunoprecipitated by anti DmIKK antibodies. Deletion of the ubl motif results in a decreased interaction. 3C physical binding occurs between endogenous DmIKK3 and endogenous DIAP1 in S2 cells The interaction was not detected by GST pull down as stated by authors. ik2-th-1 1 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey ubl domain required to bind necessary binding region required to bind deletion analysis deletion analysis range range range range region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 3E physical binding occurs between endogenous DmIKK3 and endogenous DIAP1 in S2 cells The interaction was not detected by GST pull down as stated by authors. ik2-th-2 3 5 enzyme target enzyme target substrate substrate unspecified role unspecified role region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 7 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q86PE7:p.Gly19Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G R Wed May 02 16:46:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly19arg' Tue Jun 30 19:06:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly19Arg' Q86PE7:p.Asp160Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain D R Wed May 02 16:23:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp160arg' Tue Jun 30 17:14:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp160Arg' Q86PE7:p.Gly250Asp mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G D Wed May 02 14:31:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly250asp' Tue Jun 30 16:56:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly250Asp' ubl region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation 3F Wildtype DmIKK3 was autophosphorylated and induced the phosphorylation of DIAP1 in S2 cells, whereas none of the four mutant DmIKK3s generated from the alleles DmIKK3G19R, DmIKK3D160N,DmIKK3G250D, or DmIKK3DUbl did so DIAP1 (th) was found to be phosphorylated. Radioactive p32 was used to indicate phsphorylation. ik2 3 7 putative self putative self putative self putative self region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation true 3F Wildtype DmIKK3 was autophosphorylated and induced the phosphorylation of DIAP1 in S2 cells, whereas none of the four mutant DmIKK3s generated from the alleles DmIKK3G19R, DmIKK3D160N,DmIKK3G250D, or DmIKK3DUbl did so IKK epsilon was found to be auto-phosphorylated. Radioactive p32 was used to indicate phsphorylation. tbk1 3 6 putative self putative self putative self putative self region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation true 4D The self phosphorylation was studied using radioactive P32 tbk1-birc4 3 4 enzyme target enzyme target substrate substrate unspecified role unspecified role in vitro In vitro 6 enzyme enzyme unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. phosphorylation phosphorylation reaction phosphorylation 4D We also observed that NAK phosphorylated XIAP in vitro The BIRC4 or XIAP was phosphorylated as indicated by radioactive P32.