IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Emilin1 links TGF-beta maturation to blood pressure homeostasis. Emilin1 links TGF-beta maturation to blood pressure homeostasis. Cell (0092-8674) 2006 imex curation Zacchigna L., Vecchione C., Notte A., Cordenonsi M., Dupont S., Maretto S., Cifelli G., Ferrari A., Maffei A., Fabbro C., Braghetta P., Marino G., Selvetella G., Aretini A., Colonnese C., Bettarini U., Russo G., Soligo S., Adorno M., Bonaldo P., Volpin D., Piccolo S., Lembo G., Bressan GM. Apoptosis - Interactions involving proteins with a function related to apoptosis piccolo@civ.bio.unipd.it,lembo@neuromed.it,bressan@civ.bio.unipd.it 317..320 317..330 2006-07-04: Only interaction involving solely Proteins were exported. Only protein-protein interactions 5 in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Zacchigna L., Vecchione C., Notte A., Cordenonsi M., Dupont S., Maretto S., Cifelli G., Ferrari A., Maffei A., Fabbro C., Braghetta P., Marino G., Selvetella G., Aretini A., Colonnese C., Bettarini U., Russo G., Soligo S., Adorno M., Bonaldo P., Volpin D., Piccolo S., Lembo G., Bressan GM. Apoptosis - Interactions involving proteins with a function related to apoptosis Radio-receptor binding assays was carried out using monolayers of HEK293T cells transfected with HA-tagged type II TGF-beta; Receptor expression plasmid in the presence of a GPI-anchored EMI-domain. 125I-labeled TGF-beta1 (20 pM) was added to transfected cells and washed; the bound ligand was crosslinked with DSS and then immunoprecipitated with anti-HA antibody. Detection of TGF-beta1 was by autoradiography and that of EMi domain was by western blotting. 3A piccolo@civ.bio.unipd.it,lembo@neuromed.it,bressan@civ.bio.unipd.it Accepted 2006-MAR-21 by ORCHARD. Cell (0092-8674) 2006 317..320 317..330 2006-07-04: Only interaction involving solely Proteins were exported. Only protein-protein interactions 5 imex curation Emilin1 links TGF-beta maturation to blood pressure homeostasis. Emilin1 links TGF-beta maturation to blood pressure homeostasis. Cell (0092-8674) 2006 imex curation Zacchigna L., Vecchione C., Notte A., Cordenonsi M., Dupont S., Maretto S., Cifelli G., Ferrari A., Maffei A., Fabbro C., Braghetta P., Marino G., Selvetella G., Aretini A., Colonnese C., Bettarini U., Russo G., Soligo S., Adorno M., Bonaldo P., Volpin D., Piccolo S., Lembo G., Bressan GM. Apoptosis - Interactions involving proteins with a function related to apoptosis piccolo@civ.bio.unipd.it,lembo@neuromed.it,bressan@civ.bio.unipd.it 317..320 317..330 2006-07-04: Only interaction involving solely Proteins were exported. Only protein-protein interactions 5 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zacchigna L., Vecchione C., Notte A., Cordenonsi M., Dupont S., Maretto S., Cifelli G., Ferrari A., Maffei A., Fabbro C., Braghetta P., Marino G., Selvetella G., Aretini A., Colonnese C., Bettarini U., Russo G., Soligo S., Adorno M., Bonaldo P., Volpin D., Piccolo S., Lembo G., Bressan GM. Apoptosis - Interactions involving proteins with a function related to apoptosis 3F piccolo@civ.bio.unipd.it,lembo@neuromed.it,bressan@civ.bio.unipd.it Accepted 2006-MAR-21 by ORCHARD. Cell (0092-8674) 2006 317..320 317..330 2006-07-04: Only interaction involving solely Proteins were exported. Only protein-protein interactions 5 imex curation Emilin1 links TGF-beta maturation to blood pressure homeostasis. Emilin1 links TGF-beta maturation to blood pressure homeostasis. Cell (0092-8674) 2006 imex curation Zacchigna L., Vecchione C., Notte A., Cordenonsi M., Dupont S., Maretto S., Cifelli G., Ferrari A., Maffei A., Fabbro C., Braghetta P., Marino G., Selvetella G., Aretini A., Colonnese C., Bettarini U., Russo G., Soligo S., Adorno M., Bonaldo P., Volpin D., Piccolo S., Lembo G., Bressan GM. Apoptosis - Interactions involving proteins with a function related to apoptosis piccolo@civ.bio.unipd.it,lembo@neuromed.it,bressan@civ.bio.unipd.it 317..320 317..330 2006-07-04: Only interaction involving solely Proteins were exported. Only protein-protein interactions 5 human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zacchigna L., Vecchione C., Notte A., Cordenonsi M., Dupont S., Maretto S., Cifelli G., Ferrari A., Maffei A., Fabbro C., Braghetta P., Marino G., Selvetella G., Aretini A., Colonnese C., Bettarini U., Russo G., Soligo S., Adorno M., Bonaldo P., Volpin D., Piccolo S., Lembo G., Bressan GM. Apoptosis - Interactions involving proteins with a function related to apoptosis 3E, 3G piccolo@civ.bio.unipd.it,lembo@neuromed.it,bressan@civ.bio.unipd.it Accepted 2006-MAR-21 by ORCHARD. Cell (0092-8674) 2006 317..320 317..330 2006-07-04: Only interaction involving solely Proteins were exported. Only protein-protein interactions 5 imex curation emil1_mouse EMILIN-1 Emilin1 Elastin microfibril interface-located protein 1 protein protein mouse Mus musculus Mouse MAPRALWSCYLCCLLTIATEAASYPPRGYSLYTGGTGALSPGGPQAQNSPRPASRHRNWCAYVVTRTVSCVLEDGVETIVKPDYQPCGWGQPHCSRSIMYRSFLRPRYRVAYKTVTDMEWRCCQGYGGDDCGEGPASVLGPAPSTPLPRPRPVRPNLSGSSAGSHLSGLGGEGPVESEKVQQLERQVKSLTKELQGLRGVLQGMNGRLAEDVQRAVDTVFNGRQQPADAAARPGVHETLSEIQQQLQLLDNRVSTHDQELGHLNNHHNGGPGGGGRASGPVPVPSGPSEELLRQLERQLQESCSVCLTGLDGFRQQQQEDRERLRTLEKLMSSMEERQQQLVGPAMARRPPQECCPPELGRRVSELERRLDVVTGSLTVLSGRRGSELGGAAGQGGHPPGYTSLASRLSRLEDRFNSTLGPSEEQEKNWPGGPGRLGHWLPAAPGRLEKLEGLLANVSRELGGRMDLLEEQVAGAVRTCGQICSGAPGEQDSRVNEILSALERRVLDSEGRLQLVGSGLHEAEAAGEAQQAVLEGLQGLLSRLRERMDAQEETAAEILLRLNLTAAQLSQLEGLLQARGDEGCGACGGVQEELGRLRDGVERCSCPLLPPRGPGAGPGVGGPSRGPLDGFSVFGGSSGSALQALQGELSEVILTFSSLNDSLHELQTTVEGQGADLADLGATKDSIISEINRLQQEATEHVTESEERFRGLEEGQAQAGQCPSLEGRLGRLEGVCERLDTVAGGLQGLREGLSRHVAGLWAAVRESNSTSLTQAALLEKLLGGQAGLGRRLGALNNSLLLLEDRLQQLSLKDFTGPSGKAGPPGPPGLQGPSGPAGPPGPPGKDGQQGAIGPPGPQGEQGAEGAPAAPVPRVAFSAALSLPRSEPGTVPFDRVLLNDGGYYDPETGVFTAPLAGRYLLSAVLTGHRHEKVEAVLSRSNLGVARIDSGGYEPEGLENKPVAESQPSPGALGVFSLILPLQVGDTVCIDLVMGQLAHSEEPLTIFSGALLYEDTELEQV 2EF903204DB9C88F tgfb1_pig Transforming growth factor beta-1 proprotein TGFB1 protein protein pig Sus scrofa (Pig) Pig MPPSGLRLLPLLLPLLWLLVLTPGRPAAGLSTCKTIDMELVKRKRIEAIRGQILSKLRLASPPSQGDVPPGPLPEAVLALYNSTRDRVAGESVEPEPEPEADYYAKEVTRVLMVESGNQIYDKFKGTPHSLYMLFNTSELREAVPEPVLLSRAELRLLRLKLKVEQHVELYQKYSNDSWRYLSNRLLAPSDSPEWLSFDVTGVVRQWLTRREAIEGFRLSAHCSCDSKDNTLHVEINGFNSGRRGDLATIHGMNRPFLLLMATPLERAQHLHSSRHRRALDTNYCFSSTEKNCCVRQLYIDFRKDLGWKWIHEPKGYHANFCLGPCPYIWSLDTQYSKVLALYNQHNPGASAAPCCVPQALEPLPIVYYVGRKPKVEQLSNMIVRSCKCS DIP protein P07200 original sequence version: 103 A9FC32859BA6AF06 emil1_human EMILIN-1 EMILIN1 EMI Elastin microfibril interface-located protein 1 protein protein human Homo sapiens Human MAPRTLWSCYLCCLLTAAAGAASYPPRGFSLYTGSSGALSPGGPQAQIAPRPASRHRNWCAYVVTRTVSCVLEDGVETYVKYQPCAWGQPQCPQSIMYRRFLRPRYRVAYKTVTDMEWRCCQGYGGDDCAESPAPALGPASSTPRPLARPARPNLSGSSAGSPLSGLGGEGPGESEKVQQLEEQVQSLTKELQGLRGVLQGLSGRLAEDVQRAVETAFNGRQQPADAAARPGVHETLNEIQHQLQLLDTRVSTHDQELGHLNNHHGGSSSSGGSRAPAPASAPPGPSEELLRQLEQRLQESCSVCLAGLDGFRRQQQEDRERLRAMEKLLASVEERQRHLAGLAVGRRPPQECCSPELGRRLAELERRLDVVAGSVTVLSGRRGTELGGAAGQGGHPPGYTSLASRLSRLEDRFNSTLGPSEEQEESWPGAPGGLSHWLPAARGRLEQLGGLLANVSGELGGRLDLLEEQVAGAMQACGQLCSGAPGEQDSQVSEILSALERRVLDSEGQLRLVGSGLHTVEAAGEARQATLEGLQEVVGRLQDRVDAQDETAAEFTLRLNLTAARLGQLEGLLQAHGDEGCGACGGVQEELGRLRDGVERCSCPLLPPRGPGAGPGVGGPSRGPLDGFSVFGGSSGSALQALQGELSEVILSFSSLNDSLNELQTTVEGQGADLADLGATKDRIISEINRLQQEATEHATESEERFRGLEEGQAQAGQCPSLEGRLGRLEGVCERLDTVAGGLQGLREGLSRHVAGLWAGLRETNTTSQMQAALLEKLVGGQAGLGRRLGALNSSLQLLEDRLHQLSLKDLTGPAGEAGPPGPPGLQGPPGPAGPPGSPGKDGQEGPIGPPGPQGEQGVEGAPAAPVPQVAFSAALSLPRSEPGTVPFDRVLLNDGGYYDPETGVFTAPLAGRYLLSAVLTGHRHEKVEAVLSRSNQGVARVDSGGYEPEGLENKPVAESQPSPGTLGVFSLILPLQAGDTVCVDLVMGQLAHSEEPLTIFSGALLYGDPELEHA 6CF330238DD0EE26 tgfr2_human TGF-beta receptor type-2 TGFBR2 Transforming growth factor-beta receptor type II TGF-beta type II receptor protein protein human Homo sapiens Human MGRGLLRGLWPLHIVLWTRIASTIPPHVQKSVNNDMIVTDNNGAVKFPQLCKFCDVRFSTCDNQKSCMSNCSITSICEKPQEVCVAVWRKNDENITLETVCHDPKLPYHDFILEDAASPKCIMKEKKKPGETFFMCSCSSDECNDNIIFSEEYNTSNPDLLLVIFQVTGISLLPPLGVAISVIIIFYCYRVNRQQKLSSTWETGKTRKLMEFSEHCAIILEDDRSDISSTCANNINHNTELLPIELDTLVGKGRFAEVYKAKLKQNTSEQFETVAVKIFPYEEYASWKTEKDIFSDINLKHENILQFLTAEERKTELGKQYWLITAFHAKGNLQEYLTRHVISWEDLRKLGSSLARGIAHLHSDHTPCGRPKMPIVHRDLKSSNILVKNDLTCCLCDFGLSLRLDPTLSVDDLANSGQVGTARYMAPEVLESRMNLENVESFKQTDVYSMALVLWEMTSRCNAVGEVKDYEPPFGSKVREHPCVESMKDNVLRDRGRPEIPSFWLNHQGIQMVCETLTECWDHDPEARLTAQCVAERFSELEHLDRLSGRSCSEEKIPEDGSLNTTK DIP protein P37173 original sequence version: 163 C541DA751FFBDBEB tgfb1-emi1 1 7 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey n terminus gpires glycosylphosphatidylinositolated residue LIPID GPI-anchor amidated carboxyl end GPIRes GPIanchor glycosylphosphatidylinositol n-term range n-terminal range n-term range n-term range n-terminal range n-term range emi domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey 125i radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro association association The binding of 125I-TGF-beta was competed by cold TGF-beta1. GPI-anchored version of EMI-domain used for the interaction. Presumably the active protein was used PRO_0000033769, however it si not clear and the pro form of tgfb1 could have been used. tgfb1-tgfbr2 1 7 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey 125i radiolabelled radiolabel radiolabeled radiolabelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction tgfb1-emi1-1 3 4 unspecified role unspecified role bait bait n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range n terminus gpires glycosylphosphatidylinositolated residue LIPID GPI-anchor amidated carboxyl end GPIRes GPIanchor glycosylphosphatidylinositol n-term range n-terminal range n-term range n-term range n-terminal range n-term range emi domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey physical association physical association P07200; Q99K41 transfected Monomer and dimers of proTGf-beta1 were detected. Anti-LAP antibodies used to detect bound TGF- beta 1 proteins. Precursor is cleaved to give LAP and tgf-beta1. tgfb1-emi1-2 2 4 unspecified role unspecified role prey prey emi domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain n terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range n terminus gpires glycosylphosphatidylinositolated residue LIPID GPI-anchor amidated carboxyl end GPIRes GPIanchor glycosylphosphatidylinositol n-term range n-terminal range n-term range n-term range n-terminal range n-term range 5 unspecified role unspecified role bait bait physical association physical association P07200; Q99K41 transfected Anti-LAP antibodies used for CoIP. Precursor is cleaved to give LAP and TGF-beta1 proteins. tgfb1-emi1-3 3 6 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association P07200 transfected anti-LAP antibodies used for CoIP. P07200 overexpressed; Q9Y6C2 endogenous