UniProt UniProt http://www.uniprot.org Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase. Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase. Science (0036-8075) 2004 imex curation Cohen H.Y., Miller C., Bitterman K.J., Wall N.R., Hekking B., Kessler B., Howitz K.T., Gorospe M., de Cabo R., Sinclair D.A. Apoptosis - Interactions involving proteins with a function related to apoptosis Exp details for CoIP fig 3 according ref 21 (pmid 15023334). Origin of recombinant SIRT1 and Ku70 used in in vitro deacetylation assays (fig 3 d and e) according sequence feature numbering (mutation construct and ptm, respect.) Origin of p53 (pos. control deacetylation assay fig 3e) according sequence feature numbering (ptm). david_sinclair@hms.harvard.edu 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cohen H.Y., Miller C., Bitterman K.J., Wall N.R., Hekking B., Kessler B., Howitz K.T., Gorospe M., de Cabo R., Sinclair D.A. Apoptosis - Interactions involving proteins with a function related to apoptosis Exp details for CoIP fig 3 according ref 21 (pmid 15023334). Origin of recombinant SIRT1 and Ku70 used in in vitro deacetylation assays (fig 3 d and e) according sequence feature numbering (mutation construct and ptm, respect.) Origin of p53 (pos. control deacetylation assay fig 3e) according sequence feature numbering (ptm). david_sinclair@hms.harvard.edu Accepted 2011-NOV-28 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Science (0036-8075) 2004 Only protein-protein interactions imex curation Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase. Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase. Science (0036-8075) 2004 imex curation Cohen H.Y., Miller C., Bitterman K.J., Wall N.R., Hekking B., Kessler B., Howitz K.T., Gorospe M., de Cabo R., Sinclair D.A. Apoptosis - Interactions involving proteins with a function related to apoptosis Exp details for CoIP fig 3 according ref 21 (pmid 15023334). Origin of recombinant SIRT1 and Ku70 used in in vitro deacetylation assays (fig 3 d and e) according sequence feature numbering (mutation construct and ptm, respect.) Origin of p53 (pos. control deacetylation assay fig 3e) according sequence feature numbering (ptm). david_sinclair@hms.harvard.edu 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro deacetylase assay deacetylase assay western blot western blot Immuno blot Immuno blot Cohen H.Y., Miller C., Bitterman K.J., Wall N.R., Hekking B., Kessler B., Howitz K.T., Gorospe M., de Cabo R., Sinclair D.A. Apoptosis - Interactions involving proteins with a function related to apoptosis Exp details for CoIP fig 3 according ref 21 (pmid 15023334). Origin of recombinant SIRT1 and Ku70 used in in vitro deacetylation assays (fig 3 d and e) according sequence feature numbering (mutation construct and ptm, respect.) Origin of p53 (pos. control deacetylation assay fig 3e) according sequence feature numbering (ptm). david_sinclair@hms.harvard.edu Accepted 2011-NOV-28 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Science (0036-8075) 2004 Only protein-protein interactions imex curation Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase. Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase. Science (0036-8075) 2004 imex curation Cohen H.Y., Miller C., Bitterman K.J., Wall N.R., Hekking B., Kessler B., Howitz K.T., Gorospe M., de Cabo R., Sinclair D.A. Apoptosis - Interactions involving proteins with a function related to apoptosis Exp details for CoIP fig 3 according ref 21 (pmid 15023334). Origin of recombinant SIRT1 and Ku70 used in in vitro deacetylation assays (fig 3 d and e) according sequence feature numbering (mutation construct and ptm, respect.) Origin of p53 (pos. control deacetylation assay fig 3e) according sequence feature numbering (ptm). david_sinclair@hms.harvard.edu 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Cohen H.Y., Miller C., Bitterman K.J., Wall N.R., Hekking B., Kessler B., Howitz K.T., Gorospe M., de Cabo R., Sinclair D.A. Apoptosis - Interactions involving proteins with a function related to apoptosis Exp details for CoIP fig 3 according ref 21 (pmid 15023334). Origin of recombinant SIRT1 and Ku70 used in in vitro deacetylation assays (fig 3 d and e) according sequence feature numbering (mutation construct and ptm, respect.) Origin of p53 (pos. control deacetylation assay fig 3e) according sequence feature numbering (ptm). david_sinclair@hms.harvard.edu Accepted 2011-NOV-28 by ORCHARD Science (0036-8075) 2004 Only protein-protein interactions imex curation Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase. Calorie restriction promotes mammalian cell survival by inducing the SIRT1 deacetylase. Science (0036-8075) 2004 imex curation Cohen H.Y., Miller C., Bitterman K.J., Wall N.R., Hekking B., Kessler B., Howitz K.T., Gorospe M., de Cabo R., Sinclair D.A. Apoptosis - Interactions involving proteins with a function related to apoptosis Exp details for CoIP fig 3 according ref 21 (pmid 15023334). Origin of recombinant SIRT1 and Ku70 used in in vitro deacetylation assays (fig 3 d and e) according sequence feature numbering (mutation construct and ptm, respect.) Origin of p53 (pos. control deacetylation assay fig 3e) according sequence feature numbering (ptm). david_sinclair@hms.harvard.edu 14-Mar-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro deacetylase assay deacetylase assay predetermined predetermined participant predetermined Cohen H.Y., Miller C., Bitterman K.J., Wall N.R., Hekking B., Kessler B., Howitz K.T., Gorospe M., de Cabo R., Sinclair D.A. Apoptosis - Interactions involving proteins with a function related to apoptosis Exp details for CoIP fig 3 according ref 21 (pmid 15023334). Origin of recombinant SIRT1 and Ku70 used in in vitro deacetylation assays (fig 3 d and e) according sequence feature numbering (mutation construct and ptm, respect.) Origin of p53 (pos. control deacetylation assay fig 3e) according sequence feature numbering (ptm). david_sinclair@hms.harvard.edu Accepted 2011-NOV-28 by ORCHARD 14-Mar-2012: Contacted by JYOTI. Science (0036-8075) 2004 Only protein-protein interactions imex curation xrcc6_human X-ray repair cross-complementing protein 6 XRCC6 G22P1 ATP-dependent DNA helicase II 70 kDa subunit Lupus Ku autoantigen protein p70 70 kDa subunit of Ku antigen Thyroid-lupus autoantigen CTC box-binding factor 75 kDa subunit DNA repair protein XRCC6 X-ray repair complementing defective repair in Chinese hamster cells 6 ATP-dependent DNA helicase 2 subunit 1 5'-deoxyribose-5-phosphate lyase Ku70 protein protein human Homo sapiens Human MSGWESYYKTEGDEEAEEEQEENLEASGDYKYSGRDSLIFLVDASKAMFESQSEDELTPFDMSIQCIQSVYISKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQELDNPGAKRILELDQFKGQQGQKRFQDMMGHGSDYSLSEVLWVCANLFSDVQFKMSHKRIMLFTNEDNPHGNDSAKASRARTKAGDLRDTGIFLDLMHLKKPGGFDISLFYRDIISIAEDEDLRVHFEESSKLEDLLRKVRAKETRKRALSRLKLKLNKDIVISVGIYNLVQKALKPPPIKLYRETNEPVKTKTRTFNTSTGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD DIP protein P12956 original sequence version: 184 DIP protein P12956 original sequence version: 138 DIP protein P12956 original sequence version: 185 DIP protein P12956 original sequence version: 164 DIP protein P12956 original sequence version: 166 BBD3CD434526DFCB sir1_human NAD-dependent protein deacetylase sirtuin-1 NAD-dependent protein deacylase sirtuin-1 SIRT1 SIR2L1 SIR2-like protein 1 Regulatory protein SIR2 homolog 1 protein protein human Homo sapiens Human MADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGLERSPGEPGGAAPEREVPAAARGCPGAAAAALWREAEAEAAAAGGEQEAQATAAAGEGDNGPGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSCESDEEDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS DIP protein Q96EB6 original sequence version: 140 DIP protein Q96EB6 original sequence version: 71 DIP protein Q96EB6 original sequence version: 125 DIP protein Q96EB6 original sequence version: 115 DIP protein Q96EB6 original sequence version: 63 DIP protein Q96EB6-1 original sequence version: 102 DIP protein Q96EB6 original sequence version: 102 DIP protein Q96EB6 original sequence version: 98 DIP protein Q96EB6 original sequence version: 144 DIP protein Q96EB6 original sequence version: 120 DIP protein Q96EB6-1 original sequence version: 120 DIP protein Q96EB6 original sequence version: 113 DIP protein Q96EB6-1 original sequence version: 117 2D3BEA6D73DA229F bax_human Apoptosis regulator BAX BAX BCL2L4 Bcl-2-like protein 4 protein protein human Homo sapiens Human MDGSGEQPRGGGPTSSEQIMKTGALLLQGFIQDRAGRMGGEAPELALDPVPQDASTKKLSECLKRIGDELDSNMELQRMIAAVDTDSPREVFFRVAADMFSDGNFNWGRVVALFYFASKLVLKALCTKVPELIRTIMGWTLDFLRERLLGWIQDQGGWDGLLSYFGTPTWQTVTIFVAGVLTASLTIWKKMG DIP protein Q07812 original sequence version: 73 DIP protein Q07812 original sequence version: 156 DIP protein Q07812 original sequence version: 92 DIP protein Q07812 original sequence version: 94 DIP protein Q07812-1 original sequence version: 93 DIP protein Q07812 original sequence version: 95 DIP protein Q07812 original sequence version: 120 DIP protein Q07812 original sequence version: 126 DIP protein Q07812 original sequence version: 143 DIP protein Q07812 original sequence version: 172 DIP protein Q07812 original sequence version: 175 6C0CDB0A7DEE4994 p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 sirt1-xrcc6-1 3 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 3A xrcc6-sirt1-1 3 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 3A sirt1-xrcc6-2 2 5 enzyme target enzyme target substrate substrate neutral component neutral component lys-539 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 enzyme enzyme neutral component neutral component deacetylation deacetylation reaction deacetylation 3c sirt1-xrcc6-3 2 6 enzyme enzyme neutral component neutral component 5 enzyme target enzyme target substrate substrate neutral component neutral component lys452 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro deacetylation deacetylation reaction deacetylation 3c bax-xrcc6 1 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 4C SIRT1 (UniProt Ac Q96EB6). Interaction is decreased by siRNA reduced SIRT1 or catalytically inactive SIRT1 construct. xrcc6-bax 1 7 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 4C SIRT1 (UniProt Ac Q96EB6). Interaction is decreased by siRNA reduced SIRT1 or catalytically inactive SIRT1 construct. sirt1-tp53-9 4 6 enzyme enzyme neutral component neutral component 8 enzyme target enzyme target substrate substrate neutral component neutral component lys-320 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain deacetylation deacetylation reaction deacetylation 3e sirt1-xrcc6-4 4 6 enzyme enzyme neutral component neutral component 5 enzyme target enzyme target substrate substrate neutral component neutral component lys539 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain lys-542 naclys N-acetylated L-lysine NAcLys certain certain sequence position certain certain certain sequence position certain deacetylation deacetylation reaction deacetylation 3e