IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. J. Cell. Sci. (0021-9533) 2003 imex curation Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer 3 marikki.laiho@helsinki.fi Only protein-protein interactions human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. imaging technique imaging technique microscopy immunostaining immunostaining Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Colocalisation was found after treatments with UVC. Confocal microscopy was used to visualise. 3 marikki.laiho@helsinki.fi Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. J. Cell. Sci. (0021-9533) 2003 imex curation Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer 3 marikki.laiho@helsinki.fi Only protein-protein interactions human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Human SaOS-2 cell treated with UV radiation. 3 marikki.laiho@helsinki.fi Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. J. Cell. Sci. (0021-9533) 2003 imex curation Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer 3 marikki.laiho@helsinki.fi Only protein-protein interactions human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Human fibroblasts (WS1) expressing wild type p53 were treated with UVC to induce DNA damage. 3 marikki.laiho@helsinki.fi Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. J. Cell. Sci. (0021-9533) 2003 imex curation Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer 3 marikki.laiho@helsinki.fi Only protein-protein interactions mouse-embryonic_fibroblast Mus musculus embryonic fibroblasts embryonic_fibroblast Embryonic fibroblast Fibroblasts taken from an embryonic as opposed to a foetal or adult source. Foetus is defined as the stage at which tissue diferentiation is initiated. Tissue taken from an embryonic as opposed to a foetal or adult source. Embryo is defined as the development stage prior to organ formation. imaging technique imaging technique microscopy immunostaining immunostaining Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis p53 and mdm2 negative cells were transfected. Fluorescence microscopy was used for visualisation. 3 marikki.laiho@helsinki.fi Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. J. Cell. Sci. (0021-9533) 2003 imex curation Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer 3 marikki.laiho@helsinki.fi Only protein-protein interactions human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Human SaOS-2 cell treated with proteasomal inhibitor MG132. 3 marikki.laiho@helsinki.fi Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. J. Cell. Sci. (0021-9533) 2003 imex curation Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer 3 marikki.laiho@helsinki.fi Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis 3 marikki.laiho@helsinki.fi Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. J. Cell. Sci. (0021-9533) 2003 imex curation Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer 3 marikki.laiho@helsinki.fi Only protein-protein interactions human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma imaging technique imaging technique microscopy immunostaining immunostaining Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Colocalisation was found after treatments with UV, proteasomal inhibitor MG132 and arsenic trioxide. Fluorescence microscopy was used to visualise. 3 marikki.laiho@helsinki.fi Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. Cellular stress and DNA damage invoke temporally distinct Mdm2, p53 and PML complexes and damage-specific nuclear relocalization. J. Cell. Sci. (0021-9533) 2003 imex curation Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis Cancer - Interactions investigated in the context of cancer 3 marikki.laiho@helsinki.fi Only protein-protein interactions in vitro In vitro coip coimmunoprecipitation co-immunoprecipitation CoIp immunoprecipitation Co-IP co-immunoprecipitation CoIp immunoprecipitation Co-IP coip western blot western blot Immuno blot Immuno blot Kurki S., Latonen L., Laiho M. Apoptosis - Interactions involving proteins with a function related to apoptosis 3 marikki.laiho@helsinki.fi Annotation to indicate that this entry has been checked and accepted by a senior curator. J. Cell. Sci. (0021-9533) 2003 Only protein-protein interactions imex curation p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 mdm2_human E3 ubiquitin-protein ligase Mdm2 MDM2 p53-binding protein Mdm2 Oncoprotein Mdm2 Double minute 2 protein RING-type E3 ubiquitin transferase Mdm2 protein protein human Homo sapiens Human MCNTNMSVPTDGAVTTSQIPASEQETLVRPKPLLLKLLKSVGAQKDTYTMKEVLFYLGQYIMTKRLYDEKQQHIVYCSNDLLGDLFGVPSFSVKEHRKIYTMIYRNLVVVNQQESSDSGTSVSENRCHLEGGSDQKDLVQELQEEKPSSSHLVSRPSTSSRRRAISETEENSDELSGERQRKRHKSDSISLSFDESLALCVIREICCERSSSSESTGTPSNPDLDAGVSEHSGDWLDQDSVSDQFSVEFEVESLDSEDYSLSEEGQELSDEDDEVYQVTVYQAGESDTDSFEEDPEISLADYWKCTSCNEMNPPLPSHCNRCWALRENWLPEDKGKDKGEISEKAKLENSTQAEEGFDVPDCKKTIVNDSRESCVEENDDKITQASQSQESEDYSQPSTSSSIIYSSQEDVKEFEREETQDKEESVESSLPLNAIEPCVICQGRPKNGCIVHGKTGHLMACFTCAKKLKKRNKPCPVCRQPIQMIVLTYFP DIP protein Q00987 original sequence version: 93 DIP protein Q00987-1 original sequence version: 192 DIP protein Q00987 original sequence version: 88 DIP protein Q00987 original sequence version: 180 DIP protein Q00987 original sequence version: 188 DIP protein Q00987 original sequence version: 189 DIP protein Q00987 original sequence version: 115 DIP protein Q00987-1 original sequence version: null DIP protein Q00987 original sequence version: 149 DIP protein Q00987 original sequence version: 146 DIP protein Q00987 original sequence version: 140 DIP protein Q00987 original sequence version: 183 DIP protein Q00987 original sequence version: 145 DIP protein Q00987 original sequence version: 182 DIP protein Q00987 original sequence version: 151 DIP protein Q00987 original sequence version: 156 DIP protein Q00987-1 original sequence version: 160 DIP protein Q00987 original sequence version: 160 DIP protein Q00987 original sequence version: 168 DIP protein Q00987 original sequence version: 186 DIP protein Q00987 original sequence version: 206 F37CE163876BC983 pml_human Protein PML PML MYL RNF71 TRIM19 Tripartite motif-containing protein 19 RING finger protein 71 Promyelocytic leukemia protein PP8675 E3 SUMO-protein ligase PML RING-type E3 SUMO transferase PML protein protein human Homo sapiens Human MEPAPARSPRPQQDPARPQEPTMPPPETPSEGRQPSPSPSPTERAPASEEEFQFLRCQQCQAEAKCPKLLPCLHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSELKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLPEEAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSSSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLVFFDLKIDNETQKISQLAAVNRESKFRVVIQPEAFFSIYSKAVSLEVGLQHFLSFLSSMRRPILACYKLWGPGLPNFFRALEDINRLWEFQEAISGFLAALPLIRERVPGASSFKLKNLAQTYLARNMSERSAMAAVLAMRDLCRLLEVSPGPQLAQHVYPFSSLQCFASLQPLVQAAVLPRAEARLLALHNVSFMELLSAHRRDRQGGLKKYSRYLSLQTTTLPPAQPAFNLQALGTYFEGLLEGPALARAEGVSTPLAGRGLAERASQQS DIP protein P29590 original sequence version: 188 DIP protein P29590 original sequence version: 195 DIP protein P29590-1 original sequence version: 187 D50968A977E34287 p29590-5 Protein PML PML MYL RNF71 TRIM19 RING finger protein 71 Tripartite motif-containing protein 19 PML-IV PML-X TRIM19zeta Promyelocytic leukemia protein PP8675 E3 SUMO-protein ligase PML RING-type E3 SUMO transferase PML protein protein human Homo sapiens Human MEPAPARSPRPQQDPARPQEPTMPPPETPSEGRQPSPSPSPTERAPASEEEFQFLRCQQCQAEAKCPKLLPCLHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSELKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDLPEEAERVKAQVQALGLAEAQPMAVVQSVPGAHPVPVYAFSIKGPSYGEDVSNTTTAQKRKCSQTQCPRKVIKMESEEGKEARLARSSPEQPRPSTSKAVSPPHLDGPPSPRSPVIGSEVFLPNSNHVASGAGEAEERVVVISSSEDSDAENSSSRELDDSSSESSDLQLEGPSTLRVLDENLADPQAEDRPLVFFDLKIDNESGFSWGYPHPFLI DIP protein P29590-5 original sequence version: 188 DIP protein P29590-5 original sequence version: 195 85FBAEC9F162C8E0 mdm2-pml-1 8 11 unspecified role unspecified role prey prey in vitro In vitro 10 unspecified role unspecified role bait bait in vitro In vitro direct interaction direct interaction mdm2-pmlx-1 8 12 unspecified role unspecified role prey prey in vitro In vitro 10 unspecified role unspecified role bait bait in vitro In vitro direct interaction direct interaction mdm2-pmlx-2 8 10 unspecified role unspecified role bait bait del58-89 Mdm2 amino acid deletion 58-89 sufficient to bind sufficient binding region sufficient to bind less-than less-than greater-than greater-than in vitro In vitro 12 unspecified role unspecified role prey prey in vitro In vitro direct interaction direct interaction mdm2-pmlx-3 8 12 unspecified role unspecified role prey prey in vitro In vitro 10 unspecified role unspecified role bait bait del89-222 Mdm2 amino acid deletion 89-222 sufficient to bind sufficient binding region sufficient to bind less-than less-than greater-than greater-than in vitro In vitro direct interaction direct interaction mdm2-pmlx-4 8 10 unspecified role unspecified role bait bait del222-437 Mdm2 amino acid deletion 222-437 sufficient to bind sufficient binding region sufficient to bind less-than less-than greater-than greater-than in vitro In vitro 12 unspecified role unspecified role prey prey in vitro In vitro direct interaction direct interaction pml-mdm2-1 5 10 unspecified role unspecified role bait bait human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma 11 unspecified role unspecified role prey prey human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma physical association physical association pml-mdm2-2 2 11 unspecified role unspecified role prey prey human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma 10 unspecified role unspecified role bait bait human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma physical association physical association p53-mdm2-1 3 10 unspecified role unspecified role prey prey human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. 9 unspecified role unspecified role bait bait human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. physical association physical association p53-pml-1 3 9 unspecified role unspecified role bait bait human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. 11 unspecified role unspecified role prey prey human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. physical association physical association p53-pmlx-mdm2-1 6 12 unspecified role unspecified role prey prey in vitro In vitro 9 unspecified role unspecified role bait bait in vitro In vitro 10 unspecified role unspecified role prey prey in vitro In vitro association association mdm2-pml-2 7 10 unspecified role unspecified role neutral component neutral component human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma 11 unspecified role unspecified role neutral component neutral component human-saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma saos-2 Homo sapiens SAOS-2 cell line established from primary osteogenic sarcoma colocalization colocalization p53-pmlx-mdm2-2 6 12 unspecified role unspecified role prey prey in vitro In vitro 9 unspecified role unspecified role prey prey in vitro In vitro 10 unspecified role unspecified role bait bait in vitro In vitro association association mdm2-pml-3 1 10 unspecified role unspecified role neutral component neutral component human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. 11 unspecified role unspecified role neutral component neutral component human-ws1 Homo sapiens ws1 Human fibroblast derived from the midscapular skin of a 12 week Negro female embryo. colocalization colocalization pml-mdm2-3 4 11 unspecified role unspecified role neutral component neutral component in vitro In vitro 10 unspecified role unspecified role neutral component neutral component in vitro In vitro direct interaction direct interaction pml-mdm2-4 4 12 unspecified role unspecified role neutral component neutral component in vitro In vitro 10 unspecified role unspecified role neutral component neutral component in vitro In vitro direct interaction direct interaction