UniProt UniProt http://www.uniprot.org RAPL, a Rap1-binding molecule that mediates Rap1-induced adhesion through spatial regulation of LFA-1. RAPL, a Rap1-binding molecule that mediates Rap1-induced adhesion through spatial regulation of LFA-1. Nat. Immunol. (1529-2908) 2003 imex curation Katagiri K., Maeda A., Shimonaka M., Kinashi T. Apoptosis - Interactions involving proteins with a function related to apoptosis human leukocyte cDNA Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Katagiri K., Maeda A., Shimonaka M., Kinashi T. Apoptosis - Interactions involving proteins with a function related to apoptosis Accepted 2006-OCT-11 by DAVET. human leukocyte cDNA Nat. Immunol. (1529-2908) 2003 Only protein-protein interactions imex curation RAPL, a Rap1-binding molecule that mediates Rap1-induced adhesion through spatial regulation of LFA-1. RAPL, a Rap1-binding molecule that mediates Rap1-induced adhesion through spatial regulation of LFA-1. Nat. Immunol. (1529-2908) 2003 imex curation Katagiri K., Maeda A., Shimonaka M., Kinashi T. Apoptosis - Interactions involving proteins with a function related to apoptosis human leukocyte cDNA Only protein-protein interactions mouse-baf Mus musculus pro-B cells baf mouse pro-B cell line cannot establish identity to CABRI: ECACC 87033001 since furher info is missing. anti bait coip anti bait coimmunoprecipitation anti bait coip antibody detection identification by antibody antibody detection Katagiri K., Maeda A., Shimonaka M., Kinashi T. Apoptosis - Interactions involving proteins with a function related to apoptosis Accepted 2006-OCT-11 by DAVET. human leukocyte cDNA Nat. Immunol. (1529-2908) 2003 Only protein-protein interactions imex curation RAPL, a Rap1-binding molecule that mediates Rap1-induced adhesion through spatial regulation of LFA-1. RAPL, a Rap1-binding molecule that mediates Rap1-induced adhesion through spatial regulation of LFA-1. Nat. Immunol. (1529-2908) 2003 imex curation Katagiri K., Maeda A., Shimonaka M., Kinashi T. Apoptosis - Interactions involving proteins with a function related to apoptosis human leukocyte cDNA Only protein-protein interactions mouse-baf Mus musculus pro-B cells baf mouse pro-B cell line cannot establish identity to CABRI: ECACC 87033001 since furher info is missing. anti tag coip anti tag coimmunoprecipitation anti tag coip antibody detection identification by antibody antibody detection Katagiri K., Maeda A., Shimonaka M., Kinashi T. Apoptosis - Interactions involving proteins with a function related to apoptosis Accepted 2006-OCT-11 by DAVET. human leukocyte cDNA Nat. Immunol. (1529-2908) 2003 Only protein-protein interactions imex curation RAPL, a Rap1-binding molecule that mediates Rap1-induced adhesion through spatial regulation of LFA-1. RAPL, a Rap1-binding molecule that mediates Rap1-induced adhesion through spatial regulation of LFA-1. Nat. Immunol. (1529-2908) 2003 imex curation Katagiri K., Maeda A., Shimonaka M., Kinashi T. Apoptosis - Interactions involving proteins with a function related to apoptosis human leukocyte cDNA Only protein-protein interactions mouse-do11_10 Mus musculus T-cell hybridoma DO-11.10 do11_10 Mouse T-cell hybridoma DO-11.10 Mice carrying the MHC class II restricted rearranged T cell receptor transgene, Tg(DO11.10)10Dlo, react to ovalbumin (OVA) peptide antigen. confocal microscopy confocal microscopy antibody detection identification by antibody antibody detection Katagiri K., Maeda A., Shimonaka M., Kinashi T. Apoptosis - Interactions involving proteins with a function related to apoptosis tkinashi@mfour.med.kyoto-u.ac.jp Accepted 2006-OCT-11 by DAVET. human leukocyte cDNA Nat. Immunol. (1529-2908) 2003 Only protein-protein interactions imex curation RAPL, a Rap1-binding molecule that mediates Rap1-induced adhesion through spatial regulation of LFA-1. RAPL, a Rap1-binding molecule that mediates Rap1-induced adhesion through spatial regulation of LFA-1. Nat. Immunol. (1529-2908) 2003 imex curation Katagiri K., Maeda A., Shimonaka M., Kinashi T. Apoptosis - Interactions involving proteins with a function related to apoptosis human leukocyte cDNA Only protein-protein interactions human-jurkat Homo sapiens jurkat Human T cell leukemia anti tag coip anti tag coimmunoprecipitation anti tag coip antibody detection identification by antibody antibody detection Katagiri K., Maeda A., Shimonaka M., Kinashi T. Apoptosis - Interactions involving proteins with a function related to apoptosis Accepted 2006-OCT-11 by DAVET. human leukocyte cDNA Nat. Immunol. (1529-2908) 2003 Only protein-protein interactions imex curation RAPL, a Rap1-binding molecule that mediates Rap1-induced adhesion through spatial regulation of LFA-1. RAPL, a Rap1-binding molecule that mediates Rap1-induced adhesion through spatial regulation of LFA-1. Nat. Immunol. (1529-2908) 2003 imex curation Katagiri K., Maeda A., Shimonaka M., Kinashi T. Apoptosis - Interactions involving proteins with a function related to apoptosis human leukocyte cDNA Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Katagiri K., Maeda A., Shimonaka M., Kinashi T. Apoptosis - Interactions involving proteins with a function related to apoptosis Accepted 2006-OCT-11 by DAVET. human leukocyte cDNA Nat. Immunol. (1529-2908) 2003 Only protein-protein interactions imex curation q8www0-2 Ras association domain-containing protein 5 B RASSF5 NORE1 RAPL New ras effector 1 Regulator for cell adhesion and polarization enriched in lymphoid tissues protein protein human Homo sapiens Human MTVDSSMSSGYCSLDEELEDCFFTAKTTFFRNAQSKHLSKNVCKPVEETQRPPTLQEIKQKIDSYNTREKNCLGMKLSEDGTYTGFIKVHLKLRRPVTVPAGIRPQSIYDAIKEVNLAATTDKRTSFYLPLDAIKQLHISSTTTVSEVIQGLLKKFMVVDNPQKFALFKRIHKDGQVLFQKLSIADRPLYLRLLAGPDTEVLSFVLKENETGEVEWDAFSIPELQNFLTILEKEEQDKIQQVQKKYDKFRQKLEEALRESQGKPG Initiator Met-1 is removed. Contains a N-acetylthreonine at position 2. 66AD5D84EFA6D6CB ital_mouse Integrin alpha-L Leukocyte adhesion glycoprotein LFA-1 alpha chain Leukocyte function-associated molecule 1 alpha chain Lymphocyte antigen 15 CD11 antigen-like family member A Itgal Lfa-1 Ly-15 protein protein mouse Mus musculus Mouse MSFRIAGPRLLLLGLQLFAKAWSYNLDTRPTQSFLAQAGRHFGYQVLQIEDGVVVGAPGEGDNTGGLYHCRTSSEFCQPVSLHGSNHTSKYLGMTLATDAAKGSLLACDPGLSRTCDQNTYLSGLCYLFPQSLEGPMLQNRPAYQECMKGKVDLVFLFDGSQSLDRKDFEKILEFMKDVMRKLSNTSYQFAAVQFSTDCRTEFTFLDYVKQNKNPDVLLGSVQPMFLLTNTFRAINYVVAHVFKEESGARPDATKVLVIITDGEASDKGNISAAHDITRYIIGIGKHFVSVQKQKTLHIFASEPVEEFVKILDTFEKLKDLFTDLQRRIYAIEGTNRQDLTSFNMELSSSGISADLSKGHAVVGAVGAKDWAGGFLDLREDLQGATFVGQEPLTSDVRGGYLGYTVAWMTSRSSRPLLAAGAPRYQHVGQVLLFQAPEAGGRWNQTQKIEGTQIGSYFGGELCSVDLDQDGEAELLLIGAPLFFGEQRGGRVFTYQRRQSLFEMVSELQGDPGYPLGRFGAAITALTDINGDRLTDVAVGAPLEEQGAVYIFNGKPGGLSPQPSQRIQGAQVFPGIRWFGRSIHGVKDLGGDRLADVVVGAEGRVVVLSSRPVVDVVTELSFSPEEIPVHEVECSYSAREEQKHGVKLKACFRIKPLTPQFQGRLLANLSYTLQLDGHRMRSRGLFPDGSHELSGNTSITPDKSCLDFHFHFPICIQDLISPINVSLNFSLLEEEGTPRDQKGRAMQPILRPSIHTVTKEIPFEKNCGEDKKCEANLTLSSPARSGPLRLMSSASLAVEWTLSNSGEDAYWVRLDLDFPRGLSFRKVEMLQPHSRMPVSCEELTEGSSLLTKTLKCNVSSPIFKAGQEVSLQVMFNTLLNSSWEDFVELNGTVHCENENSSLQEDNSAATHIPVLYPVNILTKEQENSTLYISFTPKGPKTQQVQHVYQVRIQPSAYDHNMPTLEALVGVPWPHSEDPITYTWSVQTDPLVTCHSEDLKRPSSEAEQPCLPGVQFRCPIVFRREILIQVTGTVELSKEIKASSTLSLCSSLSVSFNSSKHFHLYGSKASEAQVLVKVDLIHEKEMLHVYVLSGIGGLVLLFLIFLALYKVGFFKRNLKEKMEADGGVPNGSPPEDTDPLAVPGEETKDMGCLEPSGRVTRTKA DIP protein P24063 original sequence version: 83 F19C786C4D33CC0C rap1a_human Ras-related protein Rap-1A GTP-binding protein smg p21A Ras-related protein Krev-1 C21KG G-22K RAP1A KREV1 protein protein human Homo sapiens Human MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL DIP protein P62834 original sequence version: 37 DIP protein P62834 original sequence version: 93 42C39290C98E0A92 rasf5_human Ras association domain-containing protein 5 RAPL RASSF5 NORE1 New ras effector 1 Regulator for cell adhesion and polarization enriched in lymphoid tissues protein protein human Homo sapiens Human MAMASPAIGQRPYPLLLDPEPPRYLQSLSGPELPPPPPDRSSRLCVPAPLSTAPGAREGRSARRAARGNLEPPPRASRPARPLRPGLQQRLRRRPGAPRPRDVRSIFEQPQDPRVPAERGEGHCFAELVLPGGPGWCDLCGREVLRQALRCTNCKFTCHPECRSLIQLDCSQQEGLSRDRPSPESTLTVTFSQNVCKPVEETQRPPTLQEIKQKIDSYNTREKNCLGMKLSEDGTYTGFIKVHLKLRRPVTVPAGIRPQSIYDAIKEVNLAATTDKRTSFYLPLDAIKQLHISSTTTVSEVIQGLLKKFMVVDNPQKFALFKRIHKDGQVLFQKLSIADRPLYLRLLAGPDTEVLSFVLKENETGEVEWDAFSIPELQNFLTILEKEEQDKIQQVQKKYDKFRQKLEEALRESQGKPG 4AFBC69B1325CC9E rasf5_mouse Ras association domain-containing protein 5 Rassf5 Nore1 Rapl New ras effector 1 Regulator for cell adhesion and polarization enriched in lymphoid tissues protein protein mouse Mus musculus Mouse MASPAIGQRPYPLLLDPEPPRYLQSLGGTEPPPPARPRRCIPTALIPAAGASEDRGGRRSGRRDPEPTPRDCRHARPVRPGLQPRLRLRPGSHRPRDVRSIFEQPQDPRVLAERGEGHRFVELALRGGPGWCDLCGREVLRQALRCANCKFTCHSECRSLIQLDCRQKGGPALDRRSPESTLTPTLNQNVCKAVEETQHPPTIQEIKQKIDSYNSREKHCLGMKLSEDGTYTGFIKVHLKLRRPVTVPAGIRPQSIYDAIKEVNPAATTDKRTSFYLPLDAIKQLHISSTTTVSEVIQGLLKKFMVVDNPQKFALFKRIHKDGQVLFQKLSIADYPLYLRLLAGPDTDVLSFVLKENETGEVEWDAFSIPELQNFLTILEKEEQDKIHQLQKKYNKFRQKLEEALRESQGKPG DIP protein Q5EBH1 original sequence version: 22 E6FF7DDE6BECB180 ital_human Integrin alpha-L ITGAL CD11A Leukocyte adhesion glycoprotein LFA-1 alpha chain Leukocyte function-associated molecule 1 alpha chain CD11 antigen-like family member A protein protein human Homo sapiens Human MKDSCITVMAMALLSGFFFFAPASSYNLDVRGARSFSPPRAGRHFGYRVLQVGNGVIVGAPGEGNSTGSLYQCQSGTGHCLPVTLRGSNYTSKYLGMTLATDPTDGSILACDPGLSRTCDQNTYLSGLCYLFRQNLQGPMLQGRPGFQECIKGNVDLVFLFDGSMSLQPDEFQKILDFMKDVMKKLSNTSYQFAAVQFSTSYKTEFDFSDYVKRKDPDALLKHVKHMLLLTNTFGAINYVATEVFREELGARPDATKVLIIITDGEATDSGNIDAAKDIIRYIIGIGKHFQTKESQETLHKFASKPASEFVKILDTFEKLKDLFTELQKKIYVIEGTSKQDLTSFNMELSSSGISADLSRGHAVVGAVGAKDWAGGFLDLKADLQDDTFIGNEPLTPEVRAGYLGYTVTWLPSRQKTSLLASGAPRYQHMGRVLLFQEPQGGGHWSQVQTIHGTQIGSYFGGELCGVDVDQDGETELLLIGAPLFYGEQRGGRVFIYQRRQLGFEEVSELQGDPGYPLGRFGEAITALTDINGDGLVDVAVGAPLEEQGAVYIFNGRHGGLSPQPSQRIEGTQVLSGIQWFGRSIHGVKDLEGDGLADVAVGAESQMIVLSSRPVVDMVTLMSFSPAEIPVHEVECSYSTSNKMKEGVNITICFQIKSLIPQFQGRLVANLTYTLQLDGHRTRRRGLFPGGRHELRRNIAVTTSMSCTDFSFHFPVCVQDLISPINVSLNFSLWEEEGTPRDQRAQGKDIPPILRPSLHSETWEIPFEKNCGEDKKCEANLRVSFSPARSRALRLTAFASLSVELSLSNLEEDAYWVQLDLHFPPGLSFRKVEMLKPHSQIPVSCEELPEESRLLSRALSCNVSSPIFKAGHSVALQMMFNTLVNSSWGDSVELHANVTCNNEDSDLLEDNSATTIIPILYPINILIQDQEDSTLYVSFTPKGPKIHQVKHMYQVRIQPSIHDHNIPTLEAVVGVPQPPSEGPITHQWSVQMEPPVPCHYEDLERLPDAAEPCLPGALFRCPVVFRQEILVQVIGTLELVGEIEASSMFSLCSSLSISFNSSKHFHLYGSNASLAQVVMKVDVVYEKQMLYLYVLSGIGGLLLLLLIFIVLYKVGFFKRNLKEKMEAGRGVPNGIPAEDSEQLASGQEAGDPGCLKPLHEKDSESGGGKD DIP protein P20701 original sequence version: 166 22A7AF92EF286876 q5ebh1-2 Ras association domain-containing protein 5 Rassf5 Nore1 Rapl New ras effector 1 Regulator for cell adhesion and polarization enriched in lymphoid tissues protein protein mouse Mus musculus Mouse MTVDSSMSSGYCSLDEELEDCFFTAKTTFFRNLQSKQPSKNVCKAVEETQHPPTIQEIKQKIDSYNSREKHCLGMKLSEDGTYTGFIKVHLKLRRPVTVPAGIRPQSIYDAIKEVNPAATTDKRTSFYLPLDAIKQLHISSTTTVSEVIQGLLKKFMVVDNPQKFALFKRIHKDGQVLFQKLSIADYPLYLRLLAGPDTDVLSFVLKENETGEVEWDAFSIPELQNFLTILEKEEQDKIHQLQKKYNKFRQKLEEALRESQGKPG CF0431ED3B2D23D6 rap1a-rassf5-2 1 7 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 9 unspecified role unspecified role bait bait region binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P62834:p.Gly12Val constitutive active mutation mutation certain certain sequence position certain certain certain sequence position certain G V Wed May 02 18:18:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly12val' Tue Jun 30 17:50:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly12Val' yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association rap1a-rassf5-3 6 7 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) physical association physical association 1B GTP-activation of RAP1A is necessary for the interaction. RASSF5 aa 1-222 binds to RAP1A but fails to stimulate adhesion to ICAM-1. rap1a-itgal-1 5 9 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human-jurkat Homo sapiens jurkat Human T cell leukemia 10 unspecified role unspecified role prey prey physical association physical association Interaction is enhanced by TCR cross-linking. 1d rap1a-itgal-2 3 11 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mouse-baf Mus musculus pro-B cells baf mouse pro-B cell line cannot establish identity to CABRI: ECACC 87033001 since furher info is missing. physical association physical association 1d Interaction is enhanced by SDF-1. itgal-rassf5-1 2 11 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait P20701:p.[Lys1122Ala;Lys1124Ala] mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain K A Wed May 02 19:19:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys1122ala,lys1124ala' Tue Jun 30 18:17:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys1122Ala;Lys1124Ala]' mouse-baf Mus musculus pro-B cells baf mouse pro-B cell line cannot establish identity to CABRI: ECACC 87033001 since furher info is missing. physical association physical association Interaction was enhanced by chemical cross-linker DSP. Co-expression of activated RAP1A was necessary for the interaction. 4e rap1a-rassf5-4 3 9 unspecified role unspecified role bait bait region t7 tag t7 tag MASMTGGQQMG epitope tag MASMTGGQQMG epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined mouse-baf Mus musculus pro-B cells baf mouse pro-B cell line cannot establish identity to CABRI: ECACC 87033001 since furher info is missing. 11 unspecified role unspecified role prey prey physical association physical association 4e Wild-type or mutant ITGAL(lys1122ala-lys1224ala) was co-expressed and did not alter the interaction. itgal-rassf5-2 4 8 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component colocalization colocalization 5b