IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ p53 has a direct apoptogenic role at the mitochondria. p53 has a direct apoptogenic role at the mitochondria. Mol. Cell (1097-2765) 2003 imex curation Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis umoll@notes.cc.sunysb.edu 19-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis umoll@notes.cc.sunysb.edu Accepted 2012-FEB-15 by ORCHARD 19-Jun-2012: Contacted by JYOTI. Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation p53 has a direct apoptogenic role at the mitochondria. p53 has a direct apoptogenic role at the mitochondria. Mol. Cell (1097-2765) 2003 imex curation Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis umoll@notes.cc.sunysb.edu 19-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-h1299 Homo sapiens lung lymph node carcinoma h1299 Human lung lymph node carcinoma p53 null. These cells have also been reported to secrete the peptide hormone neuromedin B (NMB), but not gastrin releasing peptide (GRP). Also known as NCI H1299 or CRL-5803. http://www.lgcpromochem.com/atcc/ ATCC number: CRL-5803. ATCC number of the cell line is CRL-5803. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis umoll@notes.cc.sunysb.edu Accepted 2012-FEB-15 by ORCHARD Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation p53 has a direct apoptogenic role at the mitochondria. p53 has a direct apoptogenic role at the mitochondria. Mol. Cell (1097-2765) 2003 imex curation Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis umoll@notes.cc.sunysb.edu 19-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-rko Homo sapiens colorectal carcinoma cell line rko Human colorectal carcinoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis The co-inmunoprecipitation was performed using purified mitochondria from this cell line. Cells were pre-treated with camptothecin (induces intrinsic apoptosis). umoll@notes.cc.sunysb.edu Accepted 2012-FEB-15 by ORCHARD 19-Jun-2012: Contacted by JYOTI. Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation p53 has a direct apoptogenic role at the mitochondria. p53 has a direct apoptogenic role at the mitochondria. Mol. Cell (1097-2765) 2003 imex curation Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis umoll@notes.cc.sunysb.edu 19-Jun-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis umoll@notes.cc.sunysb.edu Accepted 2012-FEB-15 by ORCHARD 19-Jun-2012: Contacted by JYOTI. Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation p53 has a direct apoptogenic role at the mitochondria. p53 has a direct apoptogenic role at the mitochondria. Mol. Cell (1097-2765) 2003 imex curation Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis umoll@notes.cc.sunysb.edu 19-Jun-2012: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis umoll@notes.cc.sunysb.edu Accepted 2012-FEB-15 by ORCHARD 19-Jun-2012: Contacted by JYOTI. Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation p53 has a direct apoptogenic role at the mitochondria. p53 has a direct apoptogenic role at the mitochondria. Mol. Cell (1097-2765) 2003 imex curation Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis umoll@notes.cc.sunysb.edu 19-Jun-2012: Contacted by JYOTI. Only protein-protein interactions human-ml-1 Human myeloblastic leukemia ML-1 cell line ml-1 Myeloblastic leukemia ML-1 cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mihara M., Erster S., Zaika A., Petrenko O., Chittenden T., Pancoska P., Moll U.M. Apoptosis - Interactions involving proteins with a function related to apoptosis The co-inmunoprecipitation was performed using purified mitochondria from this cell line. Cells were pre-treated with camptothecin (induces intrinsic apoptosis). umoll@notes.cc.sunysb.edu Accepted 2012-FEB-15 by ORCHARD 19-Jun-2012: Contacted by JYOTI. Mol. Cell (1097-2765) 2003 Only protein-protein interactions imex curation p53_human Cellular tumor antigen p53 TP53 P53 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 protein protein human Homo sapiens Human MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD DIP protein P04637 original sequence version: 121 DIP protein P04637 original sequence version: 115 DIP protein P04637-1 original sequence version: 224 DIP protein P04637 original sequence version: 117 DIP protein P04637-1 original sequence version: 226 DIP protein P04637 original sequence version: 226 DIP protein P04637 original sequence version: 212 DIP protein P04637 original sequence version: 220 DIP protein P04637-1 original sequence version: 220 DIP protein P04637-1 original sequence version: 145 DIP protein P04637 original sequence version: 146 DIP protein P04637 original sequence version: 145 DIP protein P04637 original sequence version: 166 DIP protein P04637-1 original sequence version: 166 DIP protein P04637 original sequence version: 169 DIP protein P04637 original sequence version: 177 DIP protein P04637 original sequence version: 175 DIP protein P04637-1 original sequence version: 175 DIP protein P04637-1 original sequence version: 172 DIP protein P04637-1 original sequence version: 169 DIP protein P04637 original sequence version: 170 DIP protein P04637 original sequence version: 172 DIP protein P04637-1 original sequence version: 194 DIP protein P04637 original sequence version: 196 DIP protein P04637 original sequence version: 185 DIP protein P04637-1 original sequence version: 191 DIP protein P04637 original sequence version: 191 DIP protein P04637 original sequence version: 180 DIP protein P04637-1 original sequence version: 197 DIP protein P04637 original sequence version: 183 DIP protein P04637-1 original sequence version: 183 DIP protein P04637-1 original sequence version: 185 DIP protein P04637 original sequence version: 199 DIP protein P04637 original sequence version: 201 DIP protein P04637 original sequence version: 189 DIP protein P04637 original sequence version: 235 DIP protein P04637 original sequence version: 230 DIP protein P04637 original sequence version: 234 DIP protein P04637-1 original sequence version: 234 DIP protein P04637-1 original sequence version: 235 DIP protein P04637 original sequence version: 239 DIP protein P04637-1 original sequence version: 212 DIP protein P04637-1 original sequence version: 213 DIP protein P04637 original sequence version: 213 DIP protein P04637 original sequence version: 206 DIP protein P04637-1 original sequence version: 188 DIP protein P04637-1 original sequence version: 228 DIP protein P04637 original sequence version: 208 DIP protein P04637 original sequence version: 240 DIP protein P04637-1 original sequence version: 240 DIP protein P04637 original sequence version: 204 DIP protein P04637-1 original sequence version: 204 DIP protein P04637-1 original sequence version: 209 DIP protein P04637-1 original sequence version: 203 DIP protein P04637 original sequence version: 221 DIP protein P04637-1 original sequence version: 152 DIP protein P04637 original sequence version: 217 DIP protein P04637-1 original sequence version: 140 DIP protein P04637 original sequence version: 215 DIP protein P04637 original sequence version: 214 DIP protein P04637-1 original sequence version: 182 DIP protein P04637 original sequence version: 182 DIP protein P04637 original sequence version: 188 DIP protein P04637 original sequence version: 192 DIP protein P04637-1 original sequence version: 192 DIP protein P04637 original sequence version: 218 DIP protein P04637-1 original sequence version: 218 AD5C149FD8106131 bcl2_human Apoptosis regulator Bcl-2 BCL2 protein protein human Homo sapiens Human MAHAGRTGYDNREIVMKYIHYKLSQRGYEWDAGDVGAAPPGAAPAPGIFSSQPGHTPHPAASRDPVARTSPLQTPAAPGAAAGPALSPVPPVVHLTLRQAGDDFSRRYRRDFAEMSSQLHLTPFTARGRFATVVEELFRDGVNWGRIVAFFEFGGVMCVESVNREMSPLVDNIALWMTEYLNRHLHTWIQDNGGWDAFVELYGPSMRPLFDFSWLSLKTLLSLALVGACITLGAYLGHK DIP protein P10415 original sequence version: 148 DIP protein P10415 original sequence version: 125 DIP protein P10415 original sequence version: 151 DIP protein P10415 original sequence version: 204 DIP protein P10415 original sequence version: 149 3C49F2B714DC9CCB q07817-1 Bcl-2-like protein 1 BCLX Apoptosis regulator Bcl-X BCL2L1 BCL2L Bcl-xL protein protein human Homo sapiens Human MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK DIP protein Q07817 original sequence version: 82 DIP protein Q07817 original sequence version: 86 DIP protein Q07817-1 original sequence version: 100 DIP protein Q07817-1 original sequence version: 108 DIP protein Q07817 original sequence version: 131 DIP protein Q07817-1 original sequence version: 110 DIP protein Q07817-1 original sequence version: 135 DIP protein Q07817-1 original sequence version: 131 DIP protein Q07817-1 original sequence version: 172 DIP protein Q07817-1 original sequence version: 174 DIP protein Q07817 original sequence version: 174 DIP protein Q07817-1 original sequence version: 189 DIP protein Q07817-1 original sequence version: 192 DIP protein Q07817 original sequence version: 190 E09D3CDD851AE9BE tp53-bcl2l1 2 7 unspecified role unspecified role bait bait mitochondria-targeting tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Mitochondrial targeting pre-sequence taken from human ornithine transcarbamylase (N-terminal aas 1 to 36). 9 unspecified role unspecified role prey prey physical association physical association 3a bcl2l1-tp53 2 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey mitochondria-targeting tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Mitochondrial targeting pre-sequence taken from human ornithine transcarbamylase (N-terminal aas 1 to 36). physical association physical association 3a bcl2l1-tp53-1 1 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey mitochondria-targeting tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Mitochondrial targeting pre-sequence taken from human ornithine transcarbamylase (N-terminal aas 1 to 36). physical association physical association 3b tp53-bcl2l1-1 1 7 unspecified role unspecified role bait bait mitochondria-targeting tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Mitochondrial targeting pre-sequence taken from human ornithine transcarbamylase (N-terminal aas 1 to 36). 9 unspecified role unspecified role prey prey physical association physical association 3b and 7d bcl2l1-tp53-2 1 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait physical association physical association 3b bcl2-tp53 1 7 unspecified role unspecified role prey prey mitochondria-targeting tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Mitochondrial targeting pre-sequence taken from human ornithine transcarbamylase (N-terminal aas 1 to 36). 8 unspecified role unspecified role bait bait physical association physical association 3d bcl2l1-tp53-3 5 9 unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction 3e left tp53-bcl2l1-2 4 7 unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 9 unspecified role unspecified role prey prey purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction 3e right bcl2l1-tp53-4 3 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 4a left tp53-bcl2l1-3 3 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 4a left, 7c bcl2l1-tp53-5 6 9 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 4a right bcl2-tp53-1 6 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association 4b right tp53-bcl2-4 6 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 4b left tp53-bcl2l1-8 1 7 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association 7d tp53-bcl2l1-12 2 9 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait P04637:p.Arg175His mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R H Wed May 02 18:14:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg175his' Tue Jun 30 17:08:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg175His' P04637:p.Arg280Lys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R K Wed May 02 20:04:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg280lys' Tue Jun 30 19:17:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg280Lys' P04637:p.Arg248Thr mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R T Wed May 02 15:01:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg248thr' Tue Jun 30 19:15:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg248Thr' P04637:p.Leu194Phe mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L F Wed May 02 18:26:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu194phe' Tue Jun 30 16:42:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu194Phe' physical association physical association 7e