UniProt UniProt http://www.uniprot.org Naked cuticle targets dishevelled to antagonize Wnt signal transduction. Naked cuticle targets dishevelled to antagonize Wnt signal transduction. Genes Dev. (0890-9369) 2001 imex curation Rousset R., Mack JA., Wharton KA., Axelrod JD., Cadigan KM., Fish MP., Nusse R., Scott MP. Apoptosis - Interactions involving proteins with a function related to apoptosis scott@cmgm.stanford.edu 13-Nov-2007: Contacted by JYOTI. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Rousset R., Mack JA., Wharton KA., Axelrod JD., Cadigan KM., Fish MP., Nusse R., Scott MP. Apoptosis - Interactions involving proteins with a function related to apoptosis scott@cmgm.stanford.edu Accepted 2007-NOV-8 by JYOTI. 13-Nov-2007: Contacted by JYOTI. Genes Dev. (0890-9369) 2001 Only protein-protein interactions imex curation Naked cuticle targets dishevelled to antagonize Wnt signal transduction. Naked cuticle targets dishevelled to antagonize Wnt signal transduction. Genes Dev. (0890-9369) 2001 imex curation Rousset R., Mack JA., Wharton KA., Axelrod JD., Cadigan KM., Fish MP., Nusse R., Scott MP. Apoptosis - Interactions involving proteins with a function related to apoptosis scott@cmgm.stanford.edu 13-Nov-2007: Contacted by JYOTI. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Rousset R., Mack JA., Wharton KA., Axelrod JD., Cadigan KM., Fish MP., Nusse R., Scott MP. Apoptosis - Interactions involving proteins with a function related to apoptosis scott@cmgm.stanford.edu Accepted 2007-NOV-8 by JYOTI. 13-Nov-2007: Contacted by JYOTI. Genes Dev. (0890-9369) 2001 Only protein-protein interactions imex curation Naked cuticle targets dishevelled to antagonize Wnt signal transduction. Naked cuticle targets dishevelled to antagonize Wnt signal transduction. Genes Dev. (0890-9369) 2001 imex curation Rousset R., Mack JA., Wharton KA., Axelrod JD., Cadigan KM., Fish MP., Nusse R., Scott MP. Apoptosis - Interactions involving proteins with a function related to apoptosis scott@cmgm.stanford.edu 13-Nov-2007: Contacted by JYOTI. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Rousset R., Mack JA., Wharton KA., Axelrod JD., Cadigan KM., Fish MP., Nusse R., Scott MP. Apoptosis - Interactions involving proteins with a function related to apoptosis scott@cmgm.stanford.edu Accepted 2007-NOV-8 by JYOTI. Genes Dev. (0890-9369) 2001 Only protein-protein interactions imex curation lt_sv40 Large T antigen protein protein sv40 sv40 SV40 MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDKGGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEIPTYGTDEWEQWWNAFNEENLFCSEEMPSSDDEATADSQHSTPPKKKRKVEDPKDFPSELLSFLSHAVFSNRTLACFAIYTTKEKAALLYKKIMEKYSVTFISRHNSYNHNILFFLTPHRHRVSAINNYAQKLCTFSFLICKGVNKEYLMYSALTRDPFSVIEESLPGGLKEHDFNPEEAEETKQVSWKLVTEYAMETKCDDVLLLLGMYLEFQYSFEMCLKCIKKEQPSHYKYHEKHYANAAIFADSKNQKTICQQAVDTVLAKKRVDSLQLTREQMLTNRFNDLLDRMDIMFGSTGSADIEEWMAGVAWLHCLLPKMDSVVYDFLKCMVYNIPKKRYWLFKGPIDSGKTTLAAALLELCGGKALNVNLPLDRLNFELGVAIDQFLVVFEDVKGTGGESRDLPSGQGINNLDNLRDYLDGSVKVNLEKKHLNKRTQIFPPGIVTMNEYSVPKTLQARFVKQIDFRPKDYLKHCLERSEFLLEKRIIQSGIALLLMLIWYRPVAEFAQSIQSRIVEWKERLDKEFSLSVYQKMKFNVAMGIGVLDWLRNSDDDDEDSQENADKNEDGGEKNMEDSGHETGIDSQSQGSFQAPQSSQSVHDHNQPYHICRGFTCFKKPPTPPPEPET 3E37D4BAFC5D59BD nkd_drome Protein naked cuticle nkd CG11614 dNkd protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAGNIVKWWKHKILGGYKQFSVQECTTDSEELMYHQVRASSSCSAPPDLLLVSERDNNIQLRSPVVNIITTPPGNASGAGSKQQSHHQTNHHSSGRSHPGHTAHPQDVSSGGSHSKHLRISSTSNGKHGKYSNMQQQLPQDEDVVDAAATMQQQQHTGHAHSRHLHHHKEERIRLEEFTCDVSVEGGKSSQPLQFSFTFYDLDGHHGKITKDDIVGIVYTIYESIGKSVVVPHCGSKTINVRLTVSPEGKSKSQPVVPVPVAAGFSSSHASKLKKLPTGLAAMSKPLAGGGVGSGGASALTTSAGNRRQHRYRPRKLIKSDDEDDDSNSEKEKDAAHAPAADQPSGSGTKATGKSHHHQSQSARYHQKNNSRAEQCCTEQNTPDNGHNTYENMLNLKCCKPEVDQVDCPSHRQHHQSHPNHQMRQQDIYMKQATQRVKMLRRARKQKYQDHCLETRQRSLSVGNDSACPNRHLQLQQPPVGHPQPQSLNHKSASGSPPLGVGGGGDMMLDGVQLRQPRPHSLTPHQHQQQNQQQQQQQRKSAECWKSALNRNDLISIIRESMEKNRLCFQLNGKPQANVSPIRQPAAQQQPQQQQRQRCNTGSKIPTLITNHSPVAQQSPLSCSPPTAEPTTPSIPAAPPAIEVNGQQHHPTHPTHPSHHNHHEHPQPHIPIYHQQLAINPAVLAAQQTHNTAHNKLNLCGYDSFLHATICGGGAAAHSPPATPSNVATVQPIPKKSQKNLLQGYQRLEQSQQQQQQQRSSKDYKNYGNLIYAKLSEQLQQKDREQRRQRHKQQQHQMLQDQPKDASRSEQRPPTSNSSSAGSKIYGDAVECAHLLASEEEDLPPSPQLTSTPSKVVSTDTLIDLNDDVGEAVAEAVTEGGKQSLEAEESGQQVEVELDTSASSSMIHRYVHEHIHHHYHHFKEQQDV 4510732C7A77AA5F p53_mouse Cellular tumor antigen p53 Tp53 P53 Trp53 Tumor suppressor p53 protein protein mouse Mus musculus Mouse MTAMEESQSDISLELPLSQETFSGLWKLLPPEDILPSPHCMDDLLLPQDVEEFFEGPSEALRVSGAPAAQDPVTETPGPVAPAPATPWPLSSFVPSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD DIP protein P02340 original sequence version: 117 DIP protein P02340 original sequence version: 165 DIP protein P02340 original sequence version: 187 DIP protein P02340 original sequence version: 201 DIP protein P02340 original sequence version: 111 DIP protein P02340 original sequence version: 141 DIP protein P02340 original sequence version: 180 DIP protein P02340 original sequence version: 163 DIP protein P02340 original sequence version: 152 ED5A607378D921D7 dsh_drome Segment polarity protein dishevelled dsh CG18361 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDADRGGGQETKVIYHIDDETTPYLVKIPIPSAQVTLRDFKLVLNKQNNNYKYFFKSMDADFGVVKEEIADDSTILPCFNGRVVSWLVSADGTNQSDNCSELPTSECELGMGLTNRKLQQQQQQHQQQQQQQQQQHQQQQQQQQQQVQPVQLAQQQQQQVLHHQKMMGNPLLQPPPLTYQSASVLSSDLDSTSLFGTESELTLDRDMTDYSSVQRLQVRKKPQRRKKRAPSMSRTSSYSSITDSTMSLNIITVSINMEAVNFLGISIVGQSNRGGDGGIYVGSIMKGGAVALDGRIEPGDMILQVNDVNFENMTNDEAVRVLREVVQKPGPIKLVVAKCWDPNPKGYFTIPRTEPVRPIDPGAWVAHTQALTSHDSIIADIAEPIKERLDQNNLEEIVKAMTKPDSGLEIRDRMWLKITIPNAFIGADAVNWVLENVEDVQDRREARRIVSAMLRSNYIKHTVNKLTFSEQCYYVVNEERNPNLLGRGHLHPHQLPHGHGGHALSHADTESITSDIGPLPNPPIYMPYSATYNPSHGYQPIQYGIAERHISSGSSSSDVLTSKDISASQSDITSVIHQANQLTIAAHGSNKSSGSSNRGGGGGGGGGGNNTNDQDVSVFNYVL 0BA24ED350666CF5 nkd-dsh-1 3 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role bait bait physical association physical association Fig 4A, 5A p53-sv40t-5 3 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined [EBI-1538146]Certain and ragged-n-terminus positions must always be strictly superior to 0 and inferior or equal to the protein sequence length. Actual positions : 390-390, sequence length 387 [EBI-1538146]72-390 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 3B positive control yeast two hybrid interaction dsh-nkd-1 2 7 unspecified role unspecified role bait bait c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role prey prey c-terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig 4B nkd-dsh-2 1 5 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecobb Escherichia coli (strain B / BL21) 7 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig 4C, 5B dsh-nkd-2 1 7 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecobb Escherichia coli (strain B / BL21) 5 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro physical association physical association Fig 4D, 5C